FastQCFastQC Report
Thu 26 May 2016
SRR936210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316684
Sequences flagged as poor quality0
Sequence length125
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86732.7386921979007464No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31020.9795253312450266No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16730.5282868727185459No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13050.41208270705182454No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13040.4117669348625128No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12880.4067145798335249No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10290.3249295828017835No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8750.27630066564777506No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6890.2175670384357909No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.20619923962056813No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG6510.20556769524194465No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA6350.20051534021295675No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6240.19704184613052758No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5790.18283209761149916No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4440.14020285205441385No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4320.13641358578267293No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT4080.12883505323919112No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4050.12788773667125589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.1199934319384623No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT3580.11304644377360398No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.10894140531255131No Hit
GAACAAAAAAACACAAAGAACATGGGACTCCCGACCCGGGGAGGTAGTGA3310.10452059466218692No Hit
GAACAAAAAAACACAAAAAACATGGGACTCCCGACCCGGGGAGGTAGTGA3290.10388905028356342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.10199441714769297No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT3200.10104710057975774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA2400.074.3680951
TACCTGG1950.067.121972
GTACAAA7100.062.846271
GAACAAA13750.060.14351
CATATAG1001.8189894E-1259.4944763
TACAAGA4050.051.4149742
GTACAGG856.186929E-848.995451
ACCTGGG2700.048.4769743
GAACATA2350.045.5702363
TACATGA4100.044.9836242
GTCTAAG802.2900658E-644.6208531
GAGAACA10300.043.321221
GTTCGAC355.4268276E-542.5296316-17
GATAGTC853.4750956E-641.9960987
ATGGGAG10500.041.929445
GGAAACA3700.041.806931
CGTCACT1002.2203494E-741.6461331
TACATAA3300.041.4658432
GACATAT1455.820766E-1141.030671
TACAAGG1603.6379788E-1240.902452
Produced by FastQC (version 0.11.5)