##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936210_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 316684 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.405801998206414 31.0 31.0 34.0 30.0 34.0 2 31.22079738793245 31.0 31.0 34.0 28.0 34.0 3 31.551268772656655 31.0 31.0 34.0 30.0 34.0 4 35.42373470083743 37.0 35.0 37.0 33.0 37.0 5 35.14312374480555 37.0 35.0 37.0 33.0 37.0 6 35.421404302080305 37.0 35.0 37.0 33.0 37.0 7 35.234956612901186 37.0 35.0 37.0 32.0 37.0 8 35.36326748430612 37.0 35.0 37.0 33.0 37.0 9 36.94015485468164 39.0 37.0 39.0 33.0 39.0 10-11 36.8414176276667 39.0 37.0 39.0 32.0 39.0 12-13 36.739576360030824 39.0 37.0 39.0 32.0 39.0 14-15 37.480370021851435 39.0 36.0 41.0 32.0 41.0 16-17 37.51578703060464 39.0 36.5 41.0 32.0 41.0 18-19 37.98946583976456 40.0 37.0 41.0 33.0 41.0 20-21 38.10205441386366 40.0 37.0 41.0 33.0 41.0 22-23 37.83839884553687 40.0 36.5 41.0 33.0 41.0 24-25 37.6631263341375 40.0 36.0 41.0 32.0 41.0 26-27 37.28438285483321 40.0 35.5 41.0 32.0 41.0 28-29 36.946599765065486 39.5 35.0 41.0 31.5 41.0 30-31 36.1703054148615 38.0 35.0 40.0 31.0 41.0 32-33 35.17799130994935 36.5 34.5 40.0 30.0 41.0 34-35 34.43244527667959 36.0 34.0 40.0 26.5 41.0 36-37 33.76987943817812 35.0 33.0 40.0 22.0 41.0 38-39 33.45355780525698 35.0 33.0 40.0 19.5 41.0 40-41 33.062693410465954 35.0 33.0 40.0 14.0 41.0 42-43 32.515400209672734 35.0 33.0 40.0 4.5 41.0 44-45 31.590020335728994 35.0 32.0 40.0 2.0 41.0 46-47 30.146131474908742 35.0 27.5 40.0 2.0 41.0 48-49 29.002897209836934 35.0 22.5 40.0 2.0 41.0 50-51 28.670231208397013 35.0 20.5 40.0 2.0 41.0 52-53 28.581665003599802 35.0 21.0 40.0 2.0 41.0 54-55 28.442387364060075 35.0 20.0 40.0 2.0 41.0 56-57 28.292905546222734 35.0 20.0 40.0 2.0 41.0 58-59 28.11000713645148 35.0 20.0 40.0 2.0 41.0 60-61 27.905329918783394 35.0 18.5 39.0 2.0 41.0 62-63 27.68484672417931 35.0 17.5 39.0 2.0 41.0 64-65 27.441569514089757 35.0 14.0 39.0 2.0 41.0 66-67 27.195916118275633 35.0 11.5 38.5 2.0 41.0 68-69 26.943890439681198 35.0 7.5 38.0 2.0 40.0 70-71 26.65275321771861 35.0 6.0 37.0 2.0 40.0 72-73 26.34672575816903 34.0 2.0 37.0 2.0 39.5 74-75 26.0965994492933 34.0 2.0 36.0 2.0 39.0 76-77 25.762004079776688 34.0 2.0 36.0 2.0 39.0 78-79 25.42953227823319 34.0 2.0 35.5 2.0 38.5 80-81 25.15625671015902 34.0 2.0 35.0 2.0 37.0 82-83 24.887120915486733 34.0 2.0 35.0 2.0 37.0 84-85 24.579936150863322 34.0 2.0 35.0 2.0 36.5 86-87 24.301218249106363 33.0 2.0 35.0 2.0 36.0 88-89 24.04056725316088 33.0 2.0 35.0 2.0 36.0 90-91 23.798259463692514 33.0 2.0 35.0 2.0 35.5 92-93 23.589993495092898 33.0 2.0 35.0 2.0 35.0 94-95 23.41105171085372 33.0 2.0 35.0 2.0 35.0 96-97 23.227431761629887 33.0 2.0 35.0 2.0 35.0 98-99 23.016519622083845 33.0 2.0 35.0 2.0 35.0 100-101 22.838545679604906 33.0 2.0 35.0 2.0 35.0 102-103 21.903457389700776 30.5 2.0 34.0 2.0 35.0 104-105 22.378689798032106 32.0 2.0 35.0 2.0 35.0 106-107 22.382018036907454 32.5 2.0 35.0 2.0 35.0 108-109 22.25416345631608 33.0 2.0 35.0 2.0 35.0 110-111 22.12071970797388 32.5 2.0 35.0 2.0 35.0 112-113 21.932036351694435 32.0 2.0 35.0 2.0 35.0 114-115 21.767724292986067 32.0 2.0 35.0 2.0 35.0 116-117 21.57717472306779 32.0 2.0 35.0 2.0 35.0 118-119 21.415707771785122 32.0 2.0 35.0 2.0 35.0 120-121 21.161108549847796 32.0 2.0 35.0 2.0 35.0 122-123 20.865327897841382 31.5 2.0 35.0 2.0 35.0 124-125 19.353769056851625 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1862415641873767 1101 2 0.6413948109629963 1101 3 0.44702723851039394 1101 4 0.2250205805803347 1101 5 0.29263801749769414 1101 6 0.3068991853039975 1101 7 0.2670703454776131 1101 8 0.3345480918938648 1101 9 0.12669364110440995 1101 10-11 0.35725843081467445 1101 12-13 0.42902682838218453 1101 14-15 0.4078029879081626 1101 16-17 0.4784269086478332 1101 18-19 0.07582100928835445 1101 20-21 -0.03883637339008317 1101 22-23 0.26308503745608647 1101 24-25 0.3383773070201528 1101 26-27 -0.05174989160775567 1101 28-29 -0.10721771453436446 1101 30-31 -0.3895847780471513 1101 32-33 -0.4436450438688553 1101 34-35 -0.1664639835335393 1101 36-37 0.11353000015120784 1101 38-39 0.10872839653266198 1101 40-41 0.09356003635486587 1101 42-43 0.3226931479361923 1101 44-45 -0.07907810379862212 1101 46-47 -0.6198803448670134 1101 48-49 -0.34219759209100076 1101 50-51 -0.3694815492428596 1101 52-53 -0.38182760124486137 1101 54-55 -0.4222474342252589 1101 56-57 -0.6059451665457551 1101 58-59 -0.45852889136958197 1101 60-61 -0.5885017481884027 1101 62-63 -0.8015541522614207 1101 64-65 -0.8135862139037542 1101 66-67 -0.7985595996232178 1101 68-69 -0.5881554446298658 1101 70-71 -0.7177266595259972 1101 72-73 -0.5471722383741806 1101 74-75 -0.3909543634155348 1101 76-77 -0.5531460739435623 1101 78-79 -0.5469269290101195 1101 80-81 -0.5234350425801715 1101 82-83 -0.4418743382336423 1101 84-85 -0.907040894108718 1101 86-87 -0.9727779397350886 1101 88-89 -0.7861937554072824 1101 90-91 -0.8211923357310198 1101 92-93 -0.5148549973018675 1101 94-95 -0.582770691515389 1101 96-97 -0.9387681267979247 1101 98-99 -0.7145260861962264 1101 100-101 -0.6157278943756879 1101 102-103 -0.6169037250214799 1101 104-105 -0.672850812739302 1101 106-107 -0.6583156038186644 1101 108-109 -0.6488135184768069 1101 110-111 -0.9000034404040989 1101 112-113 -0.499721906271656 1101 114-115 -0.8669006483215149 1101 116-117 -0.5676824057346117 1101 118-119 -0.5173012887539024 1101 120-121 -0.4870697261881958 1101 122-123 -0.546557243458885 1101 124-125 -0.23295944741775543 1102 1 0.24111168294571783 1102 2 0.609831444660248 1102 3 0.23576915301807588 1102 4 0.23968569574512344 1102 5 0.38751207205123706 1102 6 0.10721747135074366 1102 7 0.524300230141705 1102 8 0.23589225151602733 1102 9 0.2032149115106563 1102 10-11 0.34451415482986647 1102 12-13 0.193518682997329 1102 14-15 0.42714613791329015 1102 16-17 0.12650692507550332 1102 18-19 0.17548004963881425 1102 20-21 0.14686898955868344 1102 22-23 0.21847365445187705 1102 24-25 0.30185783338998107 1102 26-27 0.49483580605472355 1102 28-29 0.3356619185810743 1102 30-31 0.22205490845820464 1102 32-33 0.3451482070979779 1102 34-35 0.42642506126608026 1102 36-37 0.46822215251781785 1102 38-39 0.6165636236109293 1102 40-41 0.8428413259267202 1102 42-43 0.8705472839435942 1102 44-45 1.1309821309801187 1102 46-47 0.9164658682135141 1102 48-49 0.5540193386732248 1102 50-51 0.6944109522116761 1102 52-53 0.802996485821776 1102 54-55 0.8653886073914983 1102 56-57 0.9755878256853272 1102 58-59 0.9350570458630401 1102 60-61 0.8834514329608503 1102 62-63 1.0691036389018649 1102 64-65 0.9312566109704719 1102 66-67 0.8845211868513374 1102 68-69 0.7695869490181053 1102 70-71 0.9022958429552617 1102 72-73 0.9510887390720022 1102 74-75 1.0332002962237397 1102 76-77 1.159577239653757 1102 78-79 1.1596039242018321 1102 80-81 1.1135177963260183 1102 82-83 1.1108567613933111 1102 84-85 1.2466027110796887 1102 86-87 1.1416702788897766 1102 88-89 1.1347374024009937 1102 90-91 1.3557109634920899 1102 92-93 1.3425387362276844 1102 94-95 1.3812403115990186 1102 96-97 1.2853882440566586 1102 98-99 1.3616465240916007 1102 100-101 1.40056715336722 1102 102-103 1.2279617734555366 1102 104-105 1.3834995072579623 1102 106-107 1.2195250135878162 1102 108-109 1.09054486134454 1102 110-111 0.9201328578949557 1102 112-113 0.8713487155839879 1102 114-115 0.950463481183256 1102 116-117 1.1821635848879275 1102 118-119 0.8637221891200859 1102 120-121 0.8093191817141943 1102 122-123 0.8986025840506109 1102 124-125 1.1237647731236748 1103 1 0.2610381950994771 1103 2 0.7924786697458757 1103 3 0.5116395350883707 1103 4 0.26743483949532987 1103 5 0.2343057075382191 1103 6 0.16891733501582706 1103 7 0.259045682873392 1103 8 0.3516730183168093 1103 9 0.250379311470752 1103 10-11 0.3396634581916871 1103 12-13 0.46204651527212803 1103 14-15 0.2850065941364832 1103 16-17 0.6638410786351727 1103 18-19 0.37588198825484653 1103 20-21 0.1394911090678832 1103 22-23 0.23529937852330107 1103 24-25 0.28518798003315027 1103 26-27 0.31387406296290976 1103 28-29 0.193042293003316 1103 30-31 -0.03388896145833087 1103 32-33 0.06864602906452433 1103 34-35 -0.1986663828864792 1103 36-37 -0.345506108713181 1103 38-39 -0.4370164149618745 1103 40-41 -0.23986575351330686 1103 42-43 -0.15376854753314717 1103 44-45 -0.32347036793917994 1103 46-47 -0.06188680694360471 1103 48-49 0.11868905902037952 1103 50-51 0.08060039124336171 1103 52-53 -0.04010613114360595 1103 54-55 0.04949686064191994 1103 56-57 -0.08339988754581285 1103 58-59 -0.35193003557213487 1103 60-61 -0.3393730275186506 1103 62-63 -0.35906282270821777 1103 64-65 -0.22900965777557758 1103 66-67 -0.25679535955241306 1103 68-69 -0.4726278283569485 1103 70-71 -0.37053402104234223 1103 72-73 -0.3821690835595959 1103 74-75 -0.2932927373944558 1103 76-77 -0.2146683460876062 1103 78-79 -0.15949875926839496 1103 80-81 -0.14318672267773636 1103 82-83 -0.26816262476702946 1103 84-85 -0.15119390049880366 1103 86-87 -0.28438257583040283 1103 88-89 -0.003727749063774155 1103 90-91 3.3196904524501747E-4 1103 92-93 -0.16276710289711005 1103 94-95 0.08505062317418677 1103 96-97 0.1097863752707049 1103 98-99 0.14977665743380442 1103 100-101 0.10904735155163792 1103 102-103 0.12709471662715188 1103 104-105 0.12712335589295165 1103 106-107 0.09262131259357176 1103 108-109 -0.06637122617582847 1103 110-111 0.11006444104263124 1103 112-113 0.29014838638503804 1103 114-115 0.2585741618602775 1103 116-117 0.23228329359673694 1103 118-119 0.2878942038841572 1103 120-121 0.11208037946633098 1103 122-123 0.0877298743109769 1103 124-125 0.1864996183336629 1104 1 0.3442963648073807 1104 2 0.6920872747225921 1104 3 0.39031115915324577 1104 4 0.20150579667858892 1104 5 0.36023777050424854 1104 6 0.16426467259097421 1104 7 0.41004090369119695 1104 8 0.4023788761820555 1104 9 0.48420824858226297 1104 10-11 0.39963586871976275 1104 12-13 0.43937702979945215 1104 14-15 0.6640985903327277 1104 16-17 0.5016182487552854 1104 18-19 0.3952721629745639 1104 20-21 0.4254461904690672 1104 22-23 0.5939890418582863 1104 24-25 0.5684718618228786 1104 26-27 0.6856095468602064 1104 28-29 0.870147520718497 1104 30-31 0.8779410449492815 1104 32-33 0.8609066924362452 1104 34-35 0.9244006195337207 1104 36-37 1.1973922551005316 1104 38-39 0.8585215185213713 1104 40-41 0.659553071811871 1104 42-43 0.666165254059365 1104 44-45 1.3243802244590235 1104 46-47 1.2794635262931422 1104 48-49 1.0254235838162167 1104 50-51 0.5517640874163483 1104 52-53 0.5414889158115521 1104 54-55 0.7100116736579487 1104 56-57 0.8972262628657823 1104 58-59 0.9542273958317047 1104 60-61 0.9861990663018823 1104 62-63 0.9152708058379062 1104 64-65 0.6732681061711858 1104 66-67 0.7983958281417038 1104 68-69 0.8095439038619219 1104 70-71 0.8771263835760372 1104 72-73 0.8337185342364322 1104 74-75 0.8745887911275894 1104 76-77 1.0879363286137433 1104 78-79 0.761069273675723 1104 80-81 0.9557721420726288 1104 82-83 0.7824024288406228 1104 84-85 0.7598729060198224 1104 86-87 0.9451324453310477 1104 88-89 1.1611479146548476 1104 90-91 0.9034708836688878 1104 92-93 1.070288431118339 1104 94-95 1.2117458860292096 1104 96-97 0.8967985089245545 1104 98-99 0.8404929633830882 1104 100-101 0.9031935675902609 1104 102-103 1.0663733906999866 1104 104-105 1.0233220490661843 1104 106-107 0.9790534959574444 1104 108-109 1.0777735614138066 1104 110-111 1.0053346423723113 1104 112-113 1.0066461559639706 1104 114-115 1.0601564752130095 1104 116-117 0.993383362570416 1104 118-119 0.9673645349986337 1104 120-121 0.8394404832941689 1104 122-123 1.0761308685114734 1104 124-125 1.2100241325589742 1105 1 0.210608864310327 1105 2 0.6113379566799466 1105 3 0.2468655049911206 1105 4 0.29709514303340256 1105 5 0.4110087998423353 1105 6 0.16394655837206074 1105 7 0.35320582205513773 1105 8 0.27440550234217653 1105 9 0.31449431279362017 1105 10-11 -0.09463409891211683 1105 12-13 0.22787282671533404 1105 14-15 0.21080372231036648 1105 16-17 0.1331485372053578 1105 18-19 0.25349064382010766 1105 20-21 0.30182104035891655 1105 22-23 0.21998016647157925 1105 24-25 0.34734072718898545 1105 26-27 0.3093201901666873 1105 28-29 0.2937225678391613 1105 30-31 0.052985382118542645 1105 32-33 -0.021128602336347058 1105 34-35 0.13588692869301866 1105 36-37 -0.06874408865369475 1105 38-39 -0.3834242270985868 1105 40-41 -0.33045615350047797 1105 42-43 -0.7038711700187008 1105 44-45 -0.5078761596957087 1105 46-47 -0.6026652145312532 1105 48-49 -0.8223130335162381 1105 50-51 -0.6836344699356722 1105 52-53 -0.6425341951864532 1105 54-55 -0.5050554086779648 1105 56-57 -0.35226077795622146 1105 58-59 -0.5697743219850793 1105 60-61 -0.45818661537712657 1105 62-63 -0.3981094758522339 1105 64-65 -0.4945152739514498 1105 66-67 -0.2737962174122508 1105 68-69 -0.46022093020174637 1105 70-71 -0.49299752191222623 1105 72-73 -0.45437763169194767 1105 74-75 -0.4681037276212656 1105 76-77 -0.381353719978204 1105 78-79 -0.36637125884747945 1105 80-81 -0.24972169048794868 1105 82-83 -0.12132427923391376 1105 84-85 0.008837046594493358 1105 86-87 -0.08334673011689375 1105 88-89 0.1312829541407723 1105 90-91 0.22423539166548423 1105 92-93 0.30871106176387286 1105 94-95 0.1411662819223558 1105 96-97 0.2068147327497165 1105 98-99 0.30230533961677253 1105 100-101 0.3889281330860044 1105 102-103 0.3986755037736849 1105 104-105 0.4318860814820269 1105 106-107 0.28666199278823257 1105 108-109 0.2670376041683582 1105 110-111 0.3829600787852563 1105 112-113 0.3752729364094911 1105 114-115 0.47523588447490184 1105 116-117 0.4625044129835665 1105 118-119 0.5060551768736019 1105 120-121 0.3042610271104209 1105 122-123 0.27995261320890563 1105 124-125 0.3781952143859044 1106 1 0.19911974956034229 1106 2 0.6938478381028723 1106 3 0.4742872378476335 1106 4 0.10154492030926576 1106 5 0.49049151519461276 1106 6 0.3824906953944591 1106 7 0.46266218694628236 1106 8 0.4395742707718142 1106 9 0.23814855710003968 1106 10-11 0.42814022821003306 1106 12-13 0.3894105643400323 1106 14-15 0.3544490262817561 1106 16-17 0.32015446605910824 1106 18-19 0.24542865749724996 1106 20-21 0.32317981443507904 1106 22-23 0.1239186193230708 1106 24-25 0.3889642606603445 1106 26-27 0.5119285721228977 1106 28-29 0.5009462016932318 1106 30-31 0.15682266802104294 1106 32-33 0.3115969450631155 1106 34-35 0.4078848582996457 1106 36-37 0.5054489284741095 1106 38-39 0.6665083282687476 1106 40-41 0.5327645629811002 1106 42-43 0.3301491574798945 1106 44-45 -0.016526325954568932 1106 46-47 -0.10545710862749758 1106 48-49 0.046326780775750365 1106 50-51 -0.201715121168494 1106 52-53 -0.18069138823495123 1106 54-55 -0.0015773046563829496 1106 56-57 -0.32790228620642736 1106 58-59 -0.4011548979308728 1106 60-61 -0.16184195761942632 1106 62-63 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-0.6236665529859344 2216 74-75 -0.27066330396725036 2216 76-77 -0.06822198046402406 2216 78-79 -0.23498862712715152 2216 80-81 -0.19754777744447338 2216 82-83 -0.19734791790001793 2216 84-85 -0.2062402779382957 2216 86-87 0.0483927840624645 2216 88-89 -0.01113041552038041 2216 90-91 0.29009939258646966 2216 92-93 0.05530532861985549 2216 94-95 -0.01141248165260933 2216 96-97 0.09046145748973089 2216 98-99 -0.0811495128334343 2216 100-101 -0.26852802409577947 2216 102-103 -0.25248148613383137 2216 104-105 0.009665351599835503 2216 106-107 -0.05531582677506819 2216 108-109 -0.26390564000831773 2216 110-111 -0.28465187869584696 2216 112-113 -0.1903435492576513 2216 114-115 -0.35623117105293645 2216 116-117 -0.18380012671481083 2216 118-119 -0.26340259203541194 2216 120-121 -0.3500772053620409 2216 122-123 -0.5175242661801711 2216 124-125 -0.5973756056762625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34.0 3 32.0 4 100.0 5 266.0 6 761.0 7 1351.0 8 2158.0 9 3889.0 10 6732.0 11 8330.0 12 8442.0 13 11084.0 14 15984.0 15 11550.0 16 4003.0 17 2966.0 18 2623.0 19 2682.0 20 2695.0 21 2952.0 22 3151.0 23 3396.0 24 3709.0 25 4249.0 26 4763.0 27 5160.0 28 5745.0 29 6573.0 30 7668.0 31 9027.0 32 10706.0 33 12979.0 34 17133.0 35 24933.0 36 34024.0 37 51776.0 38 22947.0 39 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.69430269556342 15.455174527541763 14.507572286663105 14.342950490231704 2 19.316283578743278 25.538001964121626 37.16231791613643 17.98339654099867 3 17.682926829268293 32.97987899609706 36.12370691288477 13.213487261749885 4 9.238231170504353 19.53177299768855 35.18491619406095 36.04507963774615 5 7.134212813018406 43.8356900229086 34.40208547278616 14.628011691286833 6 21.6951282666633 39.14248904270503 19.03979992674085 20.12258276389082 7 19.023695545085953 39.109017190637985 24.07573480188453 17.791552462391532 8 29.044725972894113 30.38644200527971 21.63386846193682 18.934963559889354 9 26.713064127016207 9.619683975192936 22.779805736949136 40.88744616084172 10-11 23.665546727968575 26.64248904270503 28.594434830935572 21.097529398390826 12-13 28.750899950739537 28.141143853178562 30.226345505298656 12.88161069078324 14-15 23.121313359689786 26.017891652246405 36.5976809690417 14.263114019022115 16-17 14.463313587045763 26.78900733854568 38.55752106200503 20.19015801240353 18-19 13.814717510199442 19.414779401548547 50.33124502658802 16.439258061663995 20-21 15.096594712710463 18.268210582157607 53.16293213424107 13.472262570890855 22-23 14.600516603301713 18.541511412006923 52.452760480478965 14.405211504212401 24-25 13.897607709893775 19.397411931136403 53.04925414608884 13.655726212880978 26-27 14.522520872541714 18.317628929784895 53.284188654936784 13.875661542736609 28-29 14.18764446577661 17.55077616804133 54.10914349951371 14.15243586666835 30-31 14.235010294173373 18.592350734486114 53.570751916737194 13.601887054603326 32-33 13.676882949564867 19.153004256609112 52.4200780588852 14.750034734940826 34-35 13.949552235035556 18.76839373002741 52.378396129896046 14.903657905040987 36-37 15.095331623953214 18.759709994821335 50.598072526556436 15.546885854669007 38-39 15.437628677167146 18.74771065162749 49.763802402394816 16.05085826881055 40-41 14.942024226042363 19.41509517373786 49.48165995124477 16.161220648975004 42-43 15.072243634176111 19.97195832274139 47.772060321184576 17.18373772189793 44-45 15.524360684988828 20.37581222021333 45.83740594825476 18.26242114654308 46-47 16.44593999245208 20.535870782125638 43.17126358191467 19.846925643507603 48-49 17.013425044292223 21.603236411524502 40.20186516846836 21.181473375714912 50-51 17.340851528797995 22.446424172756384 39.091721563599386 21.121002734846236 52-53 16.940540421050304 22.86486631581773 38.0145297566321 22.180063506499863 54-55 16.993388714233607 23.15150060554767 37.63269546873495 22.222415211483774 56-57 18.028689398185794 22.580624787829507 36.76932429125186 22.621361522732837 58-59 18.1382107787797 21.256455883676463 37.80357061090699 22.801762726636845 60-61 18.920591713547232 21.754109516584116 37.7014496002981 21.62384916957055 62-63 20.16647904091399 21.56601452902252 37.939032419519606 20.328474010543886 64-65 19.937128069585427 21.78160293423394 39.049910713016956 19.23135828316368 66-67 20.01803113291408 21.89898460406506 38.53704671516021 19.54593754786066 68-69 20.10430136558055 22.237537439390422 38.20894061327182 19.449220581757206 70-71 19.38633568224485 22.422131451677842 38.243889961679535 19.947642904397775 72-73 19.00720619442199 22.26451975545675 37.994360094987876 20.73391395513339 74-75 18.108888583640027 22.720777816197028 38.4269368434847 20.74339675667824 76-77 17.311845952722287 22.89489140525827 39.874227142685726 19.919035499333717 78-79 17.154008412171123 22.517241161536422 39.978338027813216 20.350412398479243 80-81 16.165376733200077 23.75024867138432 39.27886877413691 20.805505821278693 82-83 16.453651674305732 23.19034619738635 38.14470914967152 22.2112929786364 84-85 16.48846476907434 22.926468823614858 38.107944246910144 22.477122160400654 86-87 16.847762589087512 22.906818490414704 37.975988606903435 22.269430313594352 88-89 17.24551162926555 22.28104439977959 38.185926149308685 22.287517821646176 90-91 16.985862745159956 22.992696120070356 37.98049140933621 22.04094972543348 92-93 16.232162762131217 23.888873100229567 37.21860661923753 22.660357518401682 94-95 16.20408015043601 24.020500221833117 37.34532863668157 22.43009099104931 96-97 17.48839911771718 23.605486321300624 35.98965512341381 22.916459437568385 98-99 17.311138513422836 23.56286541166886 35.53250042234997 23.593495652558335 100-101 17.408555310478544 23.901061164170198 35.77391092915415 22.916472596197114 102-103 17.76546597658828 23.387089134423185 35.515458871601844 23.33198601738669 104-105 17.36344761709283 23.814861888484522 35.658269718093045 23.163420776329605 106-107 17.368603037417486 24.312128671054857 35.04697908163829 23.27228920988937 108-109 17.700415878539467 24.72914212814869 33.54469352246281 24.025748470849027 110-111 17.11977845178342 24.78475940261557 33.80378646617016 24.291675679430845 112-113 17.68075756403434 24.473505944325925 32.92567579303734 24.920060698602384 114-115 17.402001424400197 25.145558855995038 32.83400368575394 24.618436033850823 116-117 17.311467125678053 24.793247479253687 33.05908455653025 24.836200838538005 118-119 17.03772247162801 25.555557310080957 32.688387340257044 24.71833287803399 120-121 17.127965123531354 25.842187453124087 31.90230546594855 25.12754195739601 122-123 17.05183510285545 25.713072147237714 31.39712244756172 25.837970302345113 124-125 16.369584748479063 26.07073439155444 31.406998280509118 26.152682579457377 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 617.0 1 459.5 2 1398.5 3 2390.0 4 1570.5 5 1043.5 6 1553.0 7 4252.0 8 6637.0 9 6943.5 10 5231.0 11 2665.0 12 1967.5 13 1755.5 14 1704.5 15 1719.0 16 1725.5 17 1753.5 18 1768.0 19 1777.0 20 1867.5 21 1935.5 22 2024.0 23 2286.5 24 2534.5 25 2737.0 26 3043.0 27 3292.5 28 3516.0 29 4056.0 30 4632.0 31 5064.5 32 5879.5 33 6909.0 34 7909.0 35 8602.0 36 9570.0 37 10765.0 38 10816.5 39 10724.5 40 10469.0 41 9673.0 42 9186.5 43 9035.5 44 9425.5 45 9735.5 46 9572.5 47 9296.5 48 9485.5 49 8899.5 50 9186.0 51 8453.0 52 5600.5 53 4453.0 54 4029.5 55 3841.5 56 3755.0 57 3443.0 58 3041.0 59 2928.0 60 3027.5 61 3141.5 62 2704.5 63 1699.5 64 1026.0 65 915.5 66 764.5 67 521.5 68 404.0 69 282.5 70 265.0 71 265.5 72 240.0 73 329.5 74 317.0 75 177.0 76 92.0 77 74.5 78 67.5 79 33.5 80 16.5 81 8.0 82 7.0 83 1.5 84 2.5 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06347021005166033 2 9.473165679352288E-4 3 0.0 4 0.0 5 0.06599638756615427 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.003947152366396787 44-45 0.011525684909878618 46-47 0.01278877366712559 48-49 0.011683571004534488 50-51 0.009473165679352288 52-53 0.007420646448825959 54-55 0.008999507395384673 56-57 0.005210241123643758 58-59 0.004894468934332015 60-61 0.0033156079877733006 62-63 0.002368291419838072 64-65 0.0026840636091498148 66-67 0.002999835798461558 68-69 0.001736747041214586 70-71 0.003947152366396787 72-73 0.0037892662717409153 74-75 7.894304732793574E-4 76-77 6.315443786234858E-4 78-79 0.0 80-81 3.157721893117429E-4 82-83 1.5788609465587145E-4 84-85 6.315443786234858E-4 86-87 3.157721893117429E-4 88-89 0.0014209748519028432 90-91 9.473165679352288E-4 92-93 3.157721893117429E-4 94-95 0.0014209748519028432 96-97 0.001736747041214586 98-99 0.0011052026625911003 100-101 0.0011052026625911003 102-103 0.0015788609465587147 104-105 4.736582839676144E-4 106-107 1.5788609465587145E-4 108-109 0.0015788609465587147 110-111 0.0011052026625911003 112-113 0.01184145709919036 114-115 0.018788445264048704 116-117 0.019419989642672192 118-119 0.013420318045749075 120-121 0.007420646448825959 122-123 0.008210076922105316 124-125 0.00647332988089073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 316684.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.46975533970772 #Duplication Level Percentage of deduplicated Percentage of total 1 62.5742022587489 18.440464311427164 2 12.155240768917558 7.164239431104824 3 7.53059168934702 6.657740839448787 4 5.135760666909543 6.053984413484736 5 3.242397616955618 4.777633224286671 6 2.1944581359964 3.8802086622626972 7 1.485116687739751 3.06362178070253 8 1.081156376572445 2.5489131121243886 9 0.7447013693933094 1.975155044144952 >10 3.4620577331076015 17.601457604425864 >50 0.24323339690975718 4.952886789354688 >100 0.12965304416775603 7.0622450139571304 >500 0.01071512761716992 2.1737757512220384 >1k 0.007500589332018945 4.478281188819138 >5k 0.0021430255234339843 5.756211238963762 >10k+ 0.0010715127617169922 3.413181594270629 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10809 3.413181594270629 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9475 2.9919414937287643 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8754 2.764269745234998 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3967 1.2526682749996843 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2871 0.906581955514014 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1904 0.6012302484495585 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1641 0.5181821626605702 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.4597643076378977 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1222 0.38587361533894987 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1121 0.3539806242184638 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 949 0.29966780765684403 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 926 0.2924050473026739 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 912 0.28798423665230954 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 647 0.2043046064846977 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 611 0.19293680766947494 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 589 0.1859898195046166 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 587 0.18535827512599312 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 572 0.18062169228631697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 556 0.1755693372573291 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 535 0.16893812128178248 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 458 0.14462366270477828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 439 0.13862399110785514 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 435 0.1373609023506082 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 406 0.12820350886056764 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 388 0.12251960945295626 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 388 0.12251960945295626 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 383 0.12094074850639755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 378 0.11936188755983883 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 0.11715148223465663 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 345 0.10894140531255131 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 342 0.10799408874461608 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 320 0.10104710057975774 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.1577218931174296E-4 0.0 14-15 0.0 0.0 0.0 6.315443786234859E-4 0.0 16-17 0.0 0.0 0.0 6.315443786234859E-4 0.0 18-19 0.0 0.0 0.0 7.894304732793574E-4 0.0 20-21 0.0 0.0 0.0 0.0011052026625911003 0.0 22-23 0.0 0.0 0.0 0.0026840636091498148 0.0 24-25 0.0 0.0 0.0 0.004105038461052658 0.0 26-27 0.0 0.0 0.0 0.006157557691578988 0.0 28-29 0.0 0.0 0.0 0.0074206464488259585 0.0 30-31 0.0 0.0 0.0 0.01610438165489889 0.0 32-33 0.0 0.0 0.0 0.03773477662275328 0.0 34-35 0.0 0.0 0.0 0.06347021005166033 0.0 36-37 0.0 0.0 0.0 0.09773149259198444 0.0 38-39 0.0 0.0 0.0 0.14146594081166083 0.0 40-41 0.0 0.0 0.0 0.1997259097396774 0.0 42-43 0.0 0.0 0.0 0.25814376476234985 0.0 44-45 0.0 0.0 0.0 0.3287188490735244 0.0 46-47 0.0 0.0 0.0 0.4076618964014601 0.0 48-49 0.0 0.0 0.0 0.49386770408356595 0.0 50-51 0.0 0.0 0.0 0.5840206641320687 0.0 52-53 0.0 0.0 0.0 0.6763840295057534 0.0 54-55 0.0 0.0 0.0 0.7894304732793573 0.0 56-57 0.0 0.0 0.0 0.9173182099506132 0.0 58-59 0.0 0.0 0.0 1.0900455975041368 0.0 60-61 0.0 0.0 0.0 1.2409847039951498 0.0 62-63 0.0 0.0 0.0 1.3949236462846244 0.0 64-65 0.0 0.0 0.0 1.5572305515908602 0.0 66-67 0.0 0.0 0.0 1.7549039421000114 0.0 68-69 0.0 0.0 0.0 2.0103636432532115 0.0 70-71 0.0 0.0 0.0 2.2732439908552373 0.0 72-73 0.0 0.0 0.0 2.511809879880259 0.0 74-75 0.0 0.0 0.0 2.7630066564777507 0.0 76-77 0.0 0.0 0.0 3.040570410882773 0.0 78-79 0.0 0.0 0.0 3.4182339492996174 0.0 80-81 0.0 0.0 0.0 3.767951648962373 0.0 82-83 0.0 0.0 0.0 4.1243005646006745 0.0 84-85 0.0 0.0 0.0 4.505121824910637 0.0 86-87 0.0 0.0 0.0 4.9386770408356595 0.0 88-89 0.0 0.0 0.0 5.427492389890238 0.0 90-91 0.0 0.0 0.0 5.904782054034937 0.0 92-93 0.0 0.0 0.0 6.4117543039749405 0.0 94-95 0.0 0.0 0.0 6.924884111606523 0.0 96-97 0.0 0.0 0.0 7.465170327518915 0.0 98-99 0.0 0.0 0.0 8.088820401409606 0.0 100-101 0.0 0.0 0.0 8.804202296295362 0.0 102-103 0.0 0.0 0.0 9.442693663083706 0.0 104-105 0.0 0.0 0.0 10.045029114195856 0.0 106-107 0.0 0.0 0.0 10.671679023885009 0.0 108-109 0.0 0.0 0.0 11.363062232383069 0.0 110-111 0.0 0.0 0.0 12.157860832880726 0.0 112-113 0.0 0.0 0.0 12.884768412676358 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 45 3.6379788E-12 105.81976 4 ATCACGC 45 3.6379788E-12 105.81976 3 TATCACG 40 1.5825208E-10 104.166336 2 TATAACG 70 0.0 102.04049 2 CACGCAG 100 0.0 77.3807 5 TAACGCA 95 0.0 75.18772 4 CATATAG 140 0.0 63.775303 3 TACCTGG 255 0.0 60.690746 2 GTATCAC 80 5.675247E-10 59.542427 1 TACAACG 80 5.693437E-10 59.52362 2 CCACGCA 50 7.4220843E-6 59.523617 4 GATACGC 40 1.7706859E-4 59.495422 9 GGGTATG 40 1.7706859E-4 59.495422 7 GGTATGC 30 0.004235013 59.49542 8 CGCAGCG 30 0.004235013 59.49542 9 GACATAT 160 0.0 55.821026 1 GATAGTC 75 2.3068424E-8 55.529057 7 GGTATCA 5755 0.0 55.35222 1 ATAACGC 140 0.0 55.27193 3 GTGGTAC 45 3.1562842E-4 52.926605 1 >>END_MODULE