FastQCFastQC Report
Thu 26 May 2016
SRR936209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878889
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG16560.18841969805060707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13810.15713019505307269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.056.9767761
GTACATG119200.052.6061171
TACATGG119450.051.1014372
ACATGGG122350.047.4102823
CATGGGA95550.046.2003364
ATGGGAG28850.044.336825
ATGGGAT35750.043.601075
GTCGGGA5500.042.186532
GTATCAA35650.041.553892
CGGGAGT5450.041.4819374
CGTATAG758.1920865E-539.662551
TGGGATA10450.037.575056
CATGGGG22850.036.451364
TCAACGC41800.036.1517525
ATGGGAC13050.036.0154235
CAACGCA42600.035.8918156
ATCAACG42500.035.836284
TACCTGG7400.035.374712
AACGCAG43750.034.404437
CGTCACT2005.2750693E-1132.7216031
Produced by FastQC (version 0.11.5)