##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936209_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 878889 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.733701297888583 31.0 30.0 33.0 16.0 34.0 2 28.78711304840543 31.0 30.0 34.0 16.0 34.0 3 28.825410262274303 31.0 30.0 34.0 16.0 34.0 4 31.87407283513618 35.0 33.0 37.0 17.0 37.0 5 32.47427945963597 35.0 33.0 37.0 23.0 37.0 6 32.10469240142953 36.0 33.0 37.0 17.0 37.0 7 32.18827747303698 36.0 33.0 37.0 17.0 37.0 8 31.97406839771575 36.0 33.0 37.0 13.0 37.0 9 33.00111845750715 38.0 33.0 39.0 10.0 39.0 10-11 33.04701162490372 38.0 33.0 39.0 11.5 39.0 12-13 33.05669999283186 38.0 33.0 39.0 11.5 39.0 14-15 34.09098475461634 39.0 33.0 41.0 12.5 41.0 16-17 34.181739673610664 39.0 33.0 41.0 14.5 41.0 18-19 34.28028909225169 39.0 33.0 41.0 17.0 41.0 20-21 34.48402528646962 39.0 33.0 41.0 18.5 41.0 22-23 34.46914342994394 39.0 33.0 41.0 20.5 41.0 24-25 34.53068874453999 39.0 33.0 41.0 23.0 41.0 26-27 34.700545802712284 39.0 33.0 41.0 24.5 41.0 28-29 34.61874821507608 39.0 33.0 41.0 24.5 41.0 30-31 34.542307390353045 39.0 33.0 41.0 24.0 41.0 32-33 34.37578693100039 39.0 33.0 41.0 24.0 41.0 34-35 34.30717815332767 38.0 33.0 40.5 24.0 41.0 36-37 34.17267311344209 38.0 32.5 40.0 23.0 41.0 38-39 33.90434628263637 38.0 32.0 40.0 22.0 41.0 40-41 33.99158938159427 38.0 32.5 40.0 22.5 41.0 42-43 33.88117213891629 38.0 32.0 40.0 21.5 41.0 44-45 33.76344908173842 38.0 32.0 40.0 20.5 41.0 46-47 33.7465834707227 38.0 32.0 40.0 21.0 41.0 48-49 33.652899854247806 38.0 32.0 40.0 20.5 41.0 50-51 33.53026889629976 38.0 31.5 40.0 19.5 41.0 52-53 33.37714432653043 38.0 31.0 40.0 18.5 41.0 54-55 33.19929365369233 38.0 31.0 40.0 17.5 41.0 56-57 32.998838306088714 37.5 31.0 40.0 14.0 41.0 58-59 32.76564219144852 37.0 31.0 40.0 11.0 41.0 60-61 32.4995636536582 37.0 31.0 40.0 8.0 41.0 62-63 32.2269467475415 36.0 31.0 40.0 6.0 41.0 64-65 31.922896406713477 36.0 30.0 39.5 2.0 41.0 66-67 31.607498785398384 35.5 30.0 39.0 2.0 41.0 68-69 31.27902670303076 35.0 30.0 39.0 2.0 40.5 70-71 30.909118785193577 35.0 30.0 38.5 2.0 40.0 72-73 30.57118987721999 35.0 30.0 37.5 2.0 40.0 74-75 30.211115965724908 35.0 29.0 37.0 2.0 39.5 76-77 29.876946918211516 34.5 29.0 37.0 2.0 39.0 78-79 29.540863522014725 34.0 29.0 36.0 2.0 39.0 80-81 29.22778758182205 34.0 29.0 36.0 2.0 37.5 82-83 28.929517265547755 34.0 29.0 35.0 2.0 37.0 84-85 28.942904052730206 34.0 29.0 35.0 2.0 37.0 86-87 28.81473371495149 34.0 29.0 35.0 2.0 36.0 88-89 28.625309339404637 34.0 29.0 35.0 2.0 36.0 90-91 28.4403496914855 34.0 29.0 35.0 2.0 36.0 92-93 28.308406408545334 34.0 29.0 35.0 2.0 36.0 94-95 28.170304213615143 34.0 28.5 35.0 2.0 35.0 96-97 28.06065157261042 34.0 28.0 35.0 2.0 35.0 98-99 27.934747163748778 34.0 28.0 35.0 2.0 35.0 100-101 27.81624300679608 34.0 28.0 35.0 2.0 35.0 102-103 26.865043253471143 32.5 25.5 34.5 2.0 35.0 104-105 27.50587047966239 34.0 27.0 35.0 2.0 35.0 106-107 27.609947900133008 34.0 27.0 35.0 2.0 35.0 108-109 27.561178942960943 34.0 27.0 35.0 2.0 35.0 110-111 27.443191916157787 34.0 27.0 35.0 2.0 35.0 112-113 27.38860709372856 34.0 27.0 35.0 2.0 35.0 114-115 27.26642442902346 34.0 27.0 35.0 2.0 35.0 116-117 27.13501932553485 34.0 26.5 35.0 2.0 35.0 118-119 26.922427633068565 34.0 25.0 35.0 2.0 35.0 120-121 26.70338632068441 34.0 25.0 35.0 2.0 35.0 122-123 26.400584715476015 34.0 25.0 35.0 2.0 35.0 124-125 24.4910062590384 31.5 13.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44944574126242287 1101 2 0.44700579561720133 1101 3 0.26754516459472555 1101 4 0.4733959103818073 1101 5 0.09903227789961733 1101 6 0.3917692594871909 1101 7 0.24877760321490427 1101 8 0.2483984754921167 1101 9 0.2381793046174252 1101 10-11 0.3310718472645817 1101 12-13 0.1630902977409434 1101 14-15 0.26238401403401923 1101 16-17 -0.010996159951950801 1101 18-19 -0.00621838682884146 1101 20-21 0.009307460132525591 1101 22-23 0.11784376282077602 1101 24-25 0.14303547565491925 1101 26-27 0.13502415330995632 1101 28-29 0.3164030472492385 1101 30-31 0.30064999405330184 1101 32-33 0.18575043589359552 1101 34-35 0.3796211731113388 1101 36-37 0.22888313358775036 1101 38-39 0.3007221720176645 1101 40-41 0.23147902590963554 1101 42-43 0.40246922487010295 1101 44-45 0.38901545799247117 1101 46-47 0.06302712194055715 1101 48-49 0.33593356605484814 1101 50-51 0.21548717537535111 1101 52-53 0.08472017670427334 1101 54-55 0.0651488049516189 1101 56-57 0.1506653426932303 1101 58-59 0.2533850749960038 1101 60-61 0.2540072139837264 1101 62-63 0.12970241620976708 1101 64-65 0.07930231509649843 1101 66-67 0.1297674688018695 1101 68-69 0.18387738867821923 1101 70-71 0.19552213004948982 1101 72-73 0.08052755558423286 1101 74-75 0.07352986601506117 1101 76-77 -0.04371280064212968 1101 78-79 0.01050134113134149 1101 80-81 -0.06929193665562039 1101 82-83 -0.12616631475459883 1101 84-85 -0.16854903002635524 1101 86-87 -0.14276476587781062 1101 88-89 -0.05851497380714932 1101 90-91 -0.14343265158853313 1101 92-93 -0.12435474588813378 1101 94-95 -0.03719777206470809 1101 96-97 -0.03952919190772164 1101 98-99 -0.09669756490494308 1101 100-101 0.05601594701417412 1101 102-103 0.2070312366475271 1101 104-105 0.15223811631956963 1101 106-107 -0.04417821709329672 1101 108-109 -0.07050680531505193 1101 110-111 -0.06324730358126374 1101 112-113 0.01053844214058941 1101 114-115 0.05592526503050976 1101 116-117 7.216938569385434E-4 1101 118-119 0.04243590819133658 1101 120-121 0.012966190259202648 1101 122-123 0.13856356643726642 1101 124-125 0.17356454614019157 1102 1 0.3774346758433147 1102 2 0.455008464793238 1102 3 0.4127696123900364 1102 4 0.2885408929625122 1102 5 0.29306744688534536 1102 6 0.27437302343571446 1102 7 0.2218758287851088 1102 8 0.18514051506869578 1102 9 0.45076751456885233 1102 10-11 0.5143193663723551 1102 12-13 0.42646033764361135 1102 14-15 0.48365001545823105 1102 16-17 0.3600903037515195 1102 18-19 0.4948003702874786 1102 20-21 0.5611293725345305 1102 22-23 0.6376517592273245 1102 24-25 0.708251587673935 1102 26-27 0.6318204942065009 1102 28-29 0.4922082945772317 1102 30-31 0.6524802206363347 1102 32-33 0.7597722913920464 1102 34-35 0.6160049470692712 1102 36-37 0.7719063209748072 1102 38-39 0.5975570604807672 1102 40-41 0.554299509244295 1102 42-43 0.8347944907484361 1102 44-45 0.7627146800766553 1102 46-47 0.6892963167661179 1102 48-49 0.7618269491005023 1102 50-51 0.6268210237560083 1102 52-53 0.720709135039975 1102 54-55 0.689079231452908 1102 56-57 0.6747529827993333 1102 58-59 0.732373141986713 1102 60-61 0.6843243891921347 1102 62-63 0.6109863309149048 1102 64-65 0.5580763189199445 1102 66-67 0.49226846083483267 1102 68-69 0.48238078210580326 1102 70-71 0.31997840150642176 1102 72-73 0.39394842536634656 1102 74-75 0.30235043465105704 1102 76-77 0.3155991090982546 1102 78-79 0.34819961950199385 1102 80-81 0.3071798488471451 1102 82-83 0.3270807238582947 1102 84-85 0.4307832980469115 1102 86-87 0.5136743783662112 1102 88-89 0.40858705361128855 1102 90-91 0.48537533779954956 1102 92-93 0.4853867748619578 1102 94-95 0.3783209707447419 1102 96-97 0.4119907645562044 1102 98-99 0.5193038862975605 1102 100-101 0.4004983811472371 1102 102-103 0.4384346002196402 1102 104-105 0.40653058221387894 1102 106-107 0.41533172132237794 1102 108-109 0.45404378350712804 1102 110-111 0.36809942638115345 1102 112-113 0.2047496126207129 1102 114-115 0.2718961049301605 1102 116-117 0.48069180599580363 1102 118-119 0.5322612941933862 1102 120-121 0.48857999547090003 1102 122-123 0.49511506010147954 1102 124-125 0.48069876131659584 1103 1 0.49856443253050386 1103 2 0.6220615579443027 1103 3 0.5251572171762895 1103 4 0.4841194348248372 1103 5 0.4816723766220292 1103 6 0.6439288123327174 1103 7 0.5102385761850243 1103 8 0.45196066533479495 1103 9 0.618978256681622 1103 10-11 0.8453811533040678 1103 12-13 0.6344452892940993 1103 14-15 0.669158641845236 1103 16-17 0.5938840335334206 1103 18-19 0.599550209412314 1103 20-21 0.520700792410004 1103 22-23 0.6180970029749346 1103 24-25 0.5925087031388188 1103 26-27 0.5007920236509946 1103 28-29 0.5968347598455921 1103 30-31 0.678677327492224 1103 32-33 0.62229604221816 1103 34-35 0.6777524752441622 1103 36-37 0.6209921043933662 1103 38-39 0.9933001732846947 1103 40-41 0.6883721074216282 1103 42-43 0.8479128818229071 1103 44-45 0.7996670523330849 1103 46-47 0.7032446280117242 1103 48-49 0.6845462719465161 1103 50-51 0.7870996964924473 1103 52-53 0.6670760195403034 1103 54-55 0.7500471129444435 1103 56-57 0.7298932159378708 1103 58-59 0.6865783426023881 1103 60-61 0.6727228987055085 1103 62-63 0.799140701501706 1103 64-65 0.7527363505732154 1103 66-67 0.8244580043841729 1103 68-69 0.7867019650165119 1103 70-71 0.8019011422974032 1103 72-73 0.7513953691730855 1103 74-75 0.7665731032656176 1103 76-77 0.6738708098530637 1103 78-79 0.5461793318398165 1103 80-81 0.7058192580439879 1103 82-83 0.6912417598943321 1103 84-85 0.6110792431232603 1103 86-87 0.611235108739443 1103 88-89 0.6407891519451461 1103 90-91 0.6005432023339665 1103 92-93 0.5991094804343255 1103 94-95 0.7198657309972702 1103 96-97 0.8246738687511304 1103 98-99 0.6735426750380391 1103 100-101 0.6621186222860587 1103 102-103 0.7163029608582754 1103 104-105 0.7185449674968503 1103 106-107 0.5613313647882343 1103 108-109 0.652870807925364 1103 110-111 0.5730239454948602 1103 112-113 0.7493835020603434 1103 114-115 0.7670731110868942 1103 116-117 0.9007536994529666 1103 118-119 0.7604121646584439 1103 120-121 0.6779104939230187 1103 122-123 0.8353608621293809 1103 124-125 0.648792379790283 1104 1 0.41200113340824274 1104 2 0.4641906356276273 1104 3 0.30352605745550676 1104 4 0.3700004563190724 1104 5 0.3177302849087127 1104 6 0.36447035805115036 1104 7 0.3095670975064664 1104 8 0.3549385836428982 1104 9 0.3653487297619975 1104 10-11 0.4534478605747907 1104 12-13 0.4613867244312928 1104 14-15 0.5274116309944574 1104 16-17 0.5100912048997941 1104 18-19 0.4456986560914231 1104 20-21 0.4469936426415373 1104 22-23 0.6470722042673458 1104 24-25 0.72481257261839 1104 26-27 0.5675511293252029 1104 28-29 0.5745867641306148 1104 30-31 0.6240076457588657 1104 32-33 0.8324257807971946 1104 34-35 0.6204926654784586 1104 36-37 0.6468107727533123 1104 38-39 0.8707817718747748 1104 40-41 0.7034506805780296 1104 42-43 0.809709438253897 1104 44-45 0.8289127673099301 1104 46-47 0.691408979383624 1104 48-49 0.6040849192269064 1104 50-51 0.5949275310007067 1104 52-53 0.7022692559422623 1104 54-55 0.6407347468514146 1104 56-57 0.4975403192276673 1104 58-59 0.5770591766268467 1104 60-61 0.6729842332828824 1104 62-63 0.5590584567024806 1104 64-65 0.5777393742790764 1104 66-67 0.36133479700978555 1104 68-69 0.36526702220774965 1104 70-71 0.5517683152188226 1104 72-73 0.5017371559240686 1104 74-75 0.5334756189888843 1104 76-77 0.4965380207867831 1104 78-79 0.368162969271566 1104 80-81 0.3317533671738495 1104 82-83 0.4154873452837826 1104 84-85 0.46364658537220294 1104 86-87 0.5040344772168766 1104 88-89 0.47421211655914774 1104 90-91 0.4410626965975659 1104 92-93 0.34146544404618595 1104 94-95 0.42314461602733644 1104 96-97 0.34845477534453906 1104 98-99 0.4109969954817494 1104 100-101 0.3912804294254606 1104 102-103 0.4416683973086144 1104 104-105 0.42619310748579053 1104 106-107 0.5804692630534909 1104 108-109 0.5604004834385314 1104 110-111 0.6264225908746397 1104 112-113 0.4857687265057109 1104 114-115 0.44226878805143954 1104 116-117 0.6258567214309885 1104 118-119 0.6201651278901856 1104 120-121 0.5252522245194662 1104 122-123 0.5962003076681164 1104 124-125 0.5046679802118774 1105 1 0.5091868996024402 1105 2 0.5410426876302203 1105 3 0.4882587875916897 1105 4 0.6176427377630382 1105 5 0.3647302540283164 1105 6 0.33074175559512753 1105 7 0.39708318104824514 1105 8 0.3091161871179722 1105 9 0.3901693661041108 1105 10-11 0.5047355732635452 1105 12-13 0.6054021028705918 1105 14-15 0.6010608708219323 1105 16-17 0.5745308800379902 1105 18-19 0.5452220685815163 1105 20-21 0.45152019463898085 1105 22-23 0.3437381503913457 1105 24-25 0.3471138829767071 1105 26-27 0.3518685180785539 1105 28-29 0.5693334933815564 1105 30-31 0.554832975921812 1105 32-33 0.6086514753488643 1105 34-35 0.6995783997629133 1105 36-37 0.6279624058118074 1105 38-39 0.7732583799716224 1105 40-41 0.6185424468339846 1105 42-43 0.820117162471341 1105 44-45 0.8250913177839436 1105 46-47 0.5659599268972357 1105 48-49 0.6984219685017337 1105 50-51 0.5435729107781597 1105 52-53 0.6292736512525678 1105 54-55 0.7236445040285417 1105 56-57 0.901336035086814 1105 58-59 0.8586987200086611 1105 60-61 0.588091439738939 1105 62-63 0.7796371554024191 1105 64-65 0.9120891874526507 1105 66-67 0.9954607272818414 1105 68-69 0.8656200881168132 1105 70-71 0.8803934168668057 1105 72-73 0.7671042724152315 1105 74-75 0.9613426068117157 1105 76-77 0.9738486467324954 1105 78-79 0.971122340958356 1105 80-81 0.9806514515596909 1105 82-83 0.8684359088304987 1105 84-85 0.9404137162742217 1105 86-87 0.9229229886786499 1105 88-89 0.9297994103038079 1105 90-91 0.9665750539043856 1105 92-93 1.0134980949226744 1105 94-95 0.987654801412674 1105 96-97 0.9270690688524503 1105 98-99 0.9038095823328725 1105 100-101 0.8777800331269887 1105 102-103 1.0069691209393277 1105 104-105 0.9254741088342335 1105 106-107 0.9685185481722272 1105 108-109 0.9065213904988205 1105 110-111 0.9547615341473445 1105 112-113 0.9811891631545713 1105 114-115 0.8982724525255996 1105 116-117 0.9000793073557034 1105 118-119 0.9415513490556116 1105 120-121 0.9517326823728673 1105 122-123 0.9530042891810524 1105 124-125 1.1141276761428678 1106 1 -0.29861451883727597 1106 2 -0.47172326981658586 1106 3 -0.19046461666362902 1106 4 -0.2554069076270338 1106 5 -0.1033548523546628 1106 6 -0.12032207419210295 1106 7 -0.22915795370352754 1106 8 -0.23840154337847963 1106 9 -0.2545114849597212 1106 10-11 -0.3005835756726256 1106 12-13 -0.11977520209394044 1106 14-15 -0.4744710440716844 1106 16-17 -0.32334146038754596 1106 18-19 -0.2788914065957897 1106 20-21 -0.36798335146030325 1106 22-23 -0.43924057301290986 1106 24-25 -4.018843563831801 1106 26-27 -1.2828832549710896 1106 28-29 -1.0718012583914884 1106 30-31 -0.8157343999647111 1106 32-33 -5.743121574296531 1106 34-35 -1.3131875576839036 1106 36-37 -0.6025340480888985 1106 38-39 -0.4171671519432749 1106 40-41 -0.9332306028114061 1106 42-43 -0.5766913481669604 1106 44-45 -0.5309370510103832 1106 46-47 -0.5255294348048949 1106 48-49 -0.4125709392996839 1106 50-51 -0.30039872042751625 1106 52-53 -0.3494497530027587 1106 54-55 -0.3267101059005384 1106 56-57 -0.09830935498411009 1106 58-59 0.017563968235620564 1106 60-61 0.1469566879658899 1106 62-63 -0.09518553963304299 1106 64-65 -0.21521577708635675 1106 66-67 -0.2985109039238303 1106 68-69 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32-33 -0.7547453462210711 2216 34-35 -0.9697744622305464 2216 36-37 -0.9815884550967198 2216 38-39 -0.9356380096372021 2216 40-41 -1.0144915274090778 2216 42-43 -0.8261584287126382 2216 44-45 -0.890417090389164 2216 46-47 -1.0174487484890165 2216 48-49 -1.2377281530501136 2216 50-51 -1.2080323772293937 2216 52-53 -1.353113385896087 2216 54-55 -1.2781149840473276 2216 56-57 -1.355350823250042 2216 58-59 -1.3203868283810252 2216 60-61 -1.6536083774310057 2216 62-63 -1.6273229434463516 2216 64-65 -1.4794107221368833 2216 66-67 -1.3744566978281547 2216 68-69 -1.2649798355365682 2216 70-71 -1.3534572652299133 2216 72-73 -1.5375975739673429 2216 74-75 -1.3321639127872658 2216 76-77 -1.1857107529420254 2216 78-79 -0.9517761180455189 2216 80-81 -1.1645246479320015 2216 82-83 -1.1285026924191754 2216 84-85 -1.3753403069914043 2216 86-87 -1.1511196649689097 2216 88-89 -1.1854051182558543 2216 90-91 -1.0506807876161162 2216 92-93 -1.0609946019209957 2216 94-95 -1.0866239240007047 2216 96-97 -1.1586546166798684 2216 98-99 -1.1859303369004444 2216 100-101 -1.2177893518809455 2216 102-103 -1.189782632019103 2216 104-105 -1.1619236771139718 2216 106-107 -1.1350255419971411 2216 108-109 -1.1490755194724471 2216 110-111 -1.0865605681972994 2216 112-113 -1.2633801816709358 2216 114-115 -1.1501795194270663 2216 116-117 -1.0646259545332732 2216 118-119 -1.0816705784954728 2216 120-121 -1.328623635309409 2216 122-123 -1.2436947243403367 2216 124-125 -1.3977422131545438 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35006.0 3 5507.0 4 3998.0 5 3529.0 6 4303.0 7 4258.0 8 3910.0 9 4347.0 10 5581.0 11 6798.0 12 7286.0 13 7174.0 14 6931.0 15 6300.0 16 5571.0 17 4968.0 18 4311.0 19 3774.0 20 3502.0 21 3607.0 22 4232.0 23 6127.0 24 10285.0 25 15744.0 26 17043.0 27 13538.0 28 14836.0 29 18008.0 30 20828.0 31 25390.0 32 32450.0 33 42596.0 34 58149.0 35 83262.0 36 134001.0 37 187622.0 38 63942.0 39 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.04446077823429 18.150683762531475 9.159311720391813 20.645543738842424 2 18.85985472551679 21.829971509452854 37.97309782361348 21.33707594141688 3 16.92252027562101 33.11388936929392 28.89776626828447 21.065824086800593 4 10.128515107209703 17.724730766823875 35.779083725402074 36.36767040056435 5 10.22653832150009 46.67019388312398 32.082536865101034 11.020730930274896 6 27.66409180744195 38.85420471211754 20.02280166984684 13.458901810593673 7 28.423057580232978 37.70307141605547 19.441492448326162 14.43237855538539 8 28.605774427535202 34.94126013369364 18.82702318304651 17.625942255724645 9 29.322458750962888 16.492332759489752 17.342516347715076 36.84269214183229 10-11 23.489360540099867 31.037821668508986 26.894966232380163 18.577851559010984 12-13 24.20128654351941 29.780689103521414 26.5510177029162 19.467006650042983 14-15 22.434796064893305 30.217680216826835 24.533428608489654 22.814095109790205 16-17 21.7264485209282 32.12188034071333 25.131476841642943 21.020194296715523 18-19 21.08828172780156 30.489411028460083 27.45014151815557 20.972165725582784 20-21 23.527587868540394 29.14796573498146 26.81356605266736 20.510880343810786 22-23 23.100156139849375 29.653132648261675 26.106332262410614 21.14037894947834 24-25 21.973681964806882 29.432563590540074 27.127545186120734 21.466209258532313 26-27 21.81691780268594 29.47536219681342 27.133442488277602 21.574277512223038 28-29 21.669696116330865 29.35329072191928 27.185513257042405 21.79149990470745 30-31 22.078060980091642 29.048831634005666 27.42272240597945 21.450384979923243 32-33 21.48944498111782 29.38087614678377 27.329323643629156 21.80035522846925 34-35 21.81767911168659 29.203223651947535 27.705918562168396 21.27317867419747 36-37 22.104389440220647 29.0100377525083 27.03616640231567 21.84940640495539 38-39 21.907281889770946 29.226890163492357 27.186677574501157 21.679150372235544 40-41 21.79413658840744 29.240084471721772 27.549682889697497 21.416096050173287 42-43 21.369811110365205 29.283810061294407 27.495838420340597 21.85054040799979 44-45 21.708382046315023 29.37547824058666 27.41586521366865 21.50027449942967 46-47 21.81227030163733 29.312298747255 26.91421939535995 21.961211555747724 48-49 21.457073451020328 29.55682484056503 26.95312811118633 22.032973597228306 50-51 21.874112856841336 29.263563796651287 27.248202103940894 21.614121242566487 52-53 21.78786110001741 29.542245388148743 26.970782032070474 21.69911147976338 54-55 21.41963143833715 29.56549046736969 27.27847842016394 21.736399674129228 56-57 21.70285392446615 29.864571569985575 26.775688306959726 21.65688619858854 58-59 21.871782122453716 29.079088247642737 27.123813403210228 21.925316226693322 60-61 21.93330587408036 29.573501669173652 27.008981906476208 21.484210550269776 62-63 21.544405322483104 29.576828572420098 27.202942851405005 21.675823253691792 64-65 21.335396571194508 29.62534596687252 27.234910439737053 21.804347022195927 66-67 21.41066317587806 30.01941665827535 26.709596084582216 21.860324081264377 68-69 21.896273354822636 29.6658728596509 26.871713883582306 21.566139901944165 70-71 21.505402036565794 29.534641473281663 27.175165252653517 21.784791237499025 72-73 21.710292017727006 29.774031869926098 26.908241692599145 21.607434419747747 74-75 21.50062693000002 30.063000207080325 26.941321172484255 21.495051690435393 76-77 21.193045734083526 30.198603912912382 26.99420288206079 21.614147470943298 78-79 21.80854150429237 29.829670548479037 26.700876678973927 21.66091126825466 80-81 21.425353061382392 29.968118659572824 26.624503347426966 21.982024931617826 82-83 21.501012929428317 29.8596007561841 26.707828599028428 21.93155771535915 84-85 21.28129099645347 29.77828776623161 26.945841729369075 21.994579507945843 86-87 21.26919759786821 30.018000059165807 26.894216087183093 21.818586255782886 88-89 21.61676442310427 29.64995420333718 27.027711247774167 21.705570125784373 90-91 21.44488673439259 29.823680103949833 26.843769751577423 21.88766341008015 92-93 21.31158079659933 29.87930175244571 26.778785872115385 22.030331578839576 94-95 20.998424139676978 29.797243097788677 27.10365975070686 22.100673011827485 96-97 21.375517133091513 29.908668265664183 26.84910636671051 21.866708234533792 98-99 21.077175320463954 30.02962855081797 26.62439326325946 22.268802865458618 100-101 20.798627802920187 30.28977138582234 26.922939073198066 21.988661738059413 102-103 21.26342977263612 30.003612562615384 26.870183842458133 21.862773822290364 104-105 20.72274776363607 30.418474835074562 26.85895262390173 21.999824777387637 106-107 20.515074247652986 30.778385746453168 26.630905365105477 22.075634640788362 108-109 20.551656340082637 31.09979115477019 26.228707998413892 22.119844506733283 110-111 20.83216233406419 31.263746203971195 26.17112253222571 21.732968929738906 112-113 20.740438590053643 31.00162763138956 26.362622164800946 21.895311613755847 114-115 20.43472176077844 31.502905958010384 26.0284064791258 22.033965802085373 116-117 20.71780033554088 31.372787737112994 26.048337684517612 21.86107424282851 118-119 20.63212845556577 31.16563048999172 26.044236248281887 22.158004806160626 120-121 20.558812854072393 31.376033843034495 25.9757009196922 22.08945238320091 122-123 21.001807410896692 31.07529285802466 25.91390306884375 22.008996662234896 124-125 20.695813049595195 31.080499109383418 25.82645862288091 22.397229218140474 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 925.0 1 1166.0 2 1501.5 3 1370.0 4 1003.5 5 789.0 6 644.5 7 577.5 8 559.0 9 553.0 10 524.0 11 485.5 12 474.0 13 479.0 14 512.0 15 579.0 16 671.0 17 766.5 18 906.5 19 1042.5 20 1284.5 21 1832.0 22 2414.0 23 3128.0 24 3830.0 25 4855.0 26 6657.0 27 8273.0 28 9397.0 29 10801.0 30 13234.0 31 15580.0 32 17821.5 33 20823.5 34 23739.0 35 25321.5 36 27163.5 37 29602.5 38 30233.5 39 29691.5 40 29021.0 41 29140.0 42 29275.5 43 29617.5 44 30812.0 45 31451.0 46 30847.0 47 29836.0 48 28744.0 49 27647.5 50 27481.0 51 26375.0 52 24417.0 53 23163.5 54 22288.0 55 21144.5 56 19352.0 57 17721.5 58 16802.0 59 15521.5 60 13899.0 61 12299.0 62 10022.0 63 7121.0 64 4911.0 65 3851.0 66 3046.0 67 2441.5 68 1976.5 69 1489.0 70 1214.0 71 1041.0 72 877.0 73 845.5 74 743.0 75 599.5 76 380.0 77 208.0 78 156.5 79 115.0 80 79.0 81 53.5 82 35.5 83 18.5 84 12.5 85 13.0 86 9.0 87 5.5 88 3.5 89 3.0 90 3.5 91 3.5 92 2.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.2756002180025008E-4 2 1.1378001090012504E-4 3 1.1378001090012504E-4 4 4.5512004360050016E-4 5 0.0010240200981011254 6 6.826800654007503E-4 7 0.0012515801199013755 8 0.0015929201526017505 9 0.002275600218002501 10-11 0.001649810158051813 12-13 0.002560050245252813 14-15 0.0035271803379038763 16-17 0.003072060294303376 18-19 0.003015170288853314 20-21 0.0038116303651541893 22-23 0.09443740904710379 24-25 0.0027307202616030013 26-27 0.0018204801744020006 28-29 0.001877370179852063 30-31 0.0013653601308015006 32-33 0.0020480401962022507 34-35 0.0011378001090012505 36-37 7.964600763008753E-4 38-39 0.0011378001090012505 40-41 0.001706700163501876 42-43 0.0018204801744020006 44-45 0.001877370179852063 46-47 0.0021618202071023757 48-49 4.5512004360050016E-4 50-51 0.001649810158051813 52-53 0.0013653601308015006 54-55 0.0014791401417016256 56-57 0.0013084701253514382 58-59 0.0011378001090012505 60-61 0.0012515801199013755 62-63 0.001536030147151688 64-65 0.0013084701253514382 66-67 5.120100490505627E-4 68-69 2.2756002180025008E-4 70-71 8.53350081750938E-4 72-73 4.5512004360050016E-4 74-75 3.4134003270037516E-4 76-77 4.5512004360050016E-4 78-79 4.5512004360050016E-4 80-81 5.689000545006253E-4 82-83 2.8445002725031263E-4 84-85 2.2756002180025008E-4 86-87 3.4134003270037516E-4 88-89 4.5512004360050016E-4 90-91 3.9823003815043763E-4 92-93 3.4134003270037516E-4 94-95 4.5512004360050016E-4 96-97 5.689000545006253E-4 98-99 7.964600763008753E-4 100-101 7.395700708508128E-4 102-103 0.0013084701253514382 104-105 7.964600763008753E-4 106-107 6.826800654007503E-4 108-109 9.671300926510628E-4 110-111 0.0011378001090012505 112-113 5.120100490505627E-4 114-115 5.689000545006253E-4 116-117 0.0010809101035511878 118-119 0.0019342601853021256 120-121 9.102400872010003E-4 122-123 8.53350081750938E-4 124-125 3.9823003815043763E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 878889.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.85254840514464 #Duplication Level Percentage of deduplicated Percentage of total 1 61.20053603422576 23.165962526563295 2 12.387940997660174 9.37830272508015 3 8.251743814083731 9.370485964483718 4 5.518021292708723 8.35484672331503 5 3.7212991716016455 7.043032851153795 6 2.379890807151188 5.405095918598945 7 1.6228488353622161 4.300027486634662 8 1.0730149269608273 3.2493079569781966 9 0.7402473105242731 2.5218222438058358 >10 2.88015298641774 18.194444517335757 >50 0.15404078839349516 3.9484753114101325 >100 0.06846141863188014 4.432724882203923 >500 0.0012010775189611514 0.29028664984489916 >1k 6.005387594805757E-4 0.3451842425916048 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1656 0.18841969805060707 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1381 0.15713019505307269 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.7067001635018755E-4 0.0 24-25 0.0 0.0 0.0 3.9823003815043763E-4 1.7067001635018758E-4 26-27 0.0 0.0 0.0 0.0011378001090012503 5.689000545006253E-4 28-29 0.0 0.0 0.0 0.0016498101580518132 5.689000545006253E-4 30-31 0.0 0.0 0.0 0.00517699049595569 5.689000545006253E-4 32-33 0.0 0.0 0.0 0.013653601308015004 6.826800654007503E-4 34-35 0.0 0.0 0.0 0.025657392457978198 6.826800654007503E-4 36-37 0.0 0.0 0.0 0.04545511435459995 6.826800654007503E-4 38-39 0.0 0.0 0.0 0.07543614722678291 6.826800654007503E-4 40-41 0.0 0.0 0.0 0.10922881046412004 6.826800654007503E-4 42-43 0.0 0.0 0.0 0.14945004431731423 6.826800654007503E-4 44-45 0.0 0.0 0.0 0.19934257909701908 6.826800654007503E-4 46-47 0.0 0.0 0.0 0.2612957950321372 6.826800654007503E-4 48-49 0.0 0.0 0.0 0.32728820135420966 0.0012515801199013755 50-51 0.0 0.0 0.0 0.39248414759998135 0.0012515801199013755 52-53 0.0 0.0 0.0 0.463141534368959 0.0014222501362515629 54-55 0.0 0.0 0.0 0.5510934827947557 0.0015929201526017505 56-57 0.0 0.0 0.0 0.6648166036894306 0.0015929201526017505 58-59 0.0 1.1378001090012504E-4 0.0 0.7985650065025276 0.0015929201526017505 60-61 0.0 1.1378001090012504E-4 0.0 0.9258279486943175 0.0015929201526017505 62-63 0.0 1.1378001090012504E-4 0.0 1.0543993610114588 0.0015929201526017505 64-65 0.0 1.1378001090012504E-4 0.0 1.2031667252633724 0.0015929201526017505 66-67 0.0 1.1378001090012504E-4 0.0 1.3915864233139792 0.0015929201526017505 68-69 0.0 1.1378001090012504E-4 0.0 1.61965845516328 0.0015929201526017505 70-71 0.0 1.1378001090012504E-4 0.0 1.8476167070016807 0.0015929201526017505 72-73 0.0 1.1378001090012504E-4 0.0 2.060043987352214 0.0021618202071023757 74-75 0.0 1.1378001090012504E-4 0.0 2.3004611503841783 0.0021618202071023757 76-77 0.0 1.1378001090012504E-4 0.0 2.5862765377652925 0.0021618202071023757 78-79 0.0 1.1378001090012504E-4 0.0 2.941895961833633 0.0021618202071023757 80-81 7.964600763008753E-4 1.1378001090012504E-4 0.0 3.308950276997437 0.002389380228902626 82-83 7.964600763008753E-4 1.1378001090012504E-4 0.0 3.6711689416979842 0.002616940250702876 84-85 0.0012515801199013755 1.1378001090012504E-4 0.0 4.043286467346844 0.002616940250702876 86-87 0.0017067001635018757 1.1378001090012504E-4 0.0 4.46779968801521 0.002616940250702876 88-89 0.0017067001635018757 1.1378001090012504E-4 0.0 4.9641080955615555 0.002616940250702876 90-91 0.0017067001635018757 1.1378001090012504E-4 0.0 5.465195263565707 0.002616940250702876 92-93 0.0017067001635018757 1.1378001090012504E-4 0.0 5.962925921248303 0.002616940250702876 94-95 0.0017067001635018757 1.1378001090012504E-4 0.0 6.511061123759656 0.002844500272503126 96-97 0.0017067001635018757 1.1378001090012504E-4 0.0 7.1093164210725135 0.002844500272503126 98-99 0.0017067001635018757 1.1378001090012504E-4 0.0 7.760081193415778 0.002844500272503126 100-101 0.0018204801744020008 1.1378001090012504E-4 0.0 8.479170862304567 0.002844500272503126 102-103 0.0019342601853021258 1.1378001090012504E-4 0.0 9.16310250782522 0.002844500272503126 104-105 0.0019342601853021258 1.1378001090012504E-4 0.0 9.83741974242481 0.002844500272503126 106-107 0.0020480401962022507 1.1378001090012504E-4 0.0 10.566635832283712 0.002844500272503126 108-109 0.0020480401962022507 1.1378001090012504E-4 0.0 11.36485949875354 0.002844500272503126 110-111 0.0020480401962022507 1.1378001090012504E-4 0.0 12.18390490721809 0.002958280283403251 112-113 0.0020480401962022507 1.1378001090012504E-4 0.0 12.990605184499977 0.003072060294303376 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2600 0.0 56.976776 1 GTACATG 11920 0.0 52.606117 1 TACATGG 11945 0.0 51.101437 2 ACATGGG 12235 0.0 47.410282 3 CATGGGA 9555 0.0 46.200336 4 ATGGGAG 2885 0.0 44.33682 5 ATGGGAT 3575 0.0 43.60107 5 GTCGGGA 550 0.0 42.18653 2 GTATCAA 3565 0.0 41.55389 2 CGGGAGT 545 0.0 41.481937 4 CGTATAG 75 8.1920865E-5 39.66255 1 TGGGATA 1045 0.0 37.57505 6 CATGGGG 2285 0.0 36.45136 4 TCAACGC 4180 0.0 36.151752 5 ATGGGAC 1305 0.0 36.015423 5 CAACGCA 4260 0.0 35.891815 6 ATCAACG 4250 0.0 35.83628 4 TACCTGG 740 0.0 35.37471 2 AACGCAG 4375 0.0 34.40443 7 CGTCACT 200 5.2750693E-11 32.721603 1 >>END_MODULE