FastQCFastQC Report
Thu 26 May 2016
SRR936209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878889
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82090.9340201094791265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59420.676080824768543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59190.6734638845178401No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14500.16498101580518132No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14480.16475345578338105No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.13835649325455204No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11770.13391907282944718No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11250.12800251226264067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9090.10342602990821366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.078.900261
GTATCAA71650.050.0979772
GTACATG99200.047.2583851
TACATGG100100.046.623092
ACATGGG101200.046.292783
CATGGGA76300.044.3141944
TACCTGG8150.043.824072
ATGGGAT29000.043.5167965
ATCACGC1002.2166387E-741.6693843
ATGGGAG20600.041.6115885
CACGCAG2300.041.4105725
TAACGCA1053.2506796E-739.685134
CATGGGG22500.039.1559944
GTATCGA650.001934227236.6324272
CCACGCA1304.1836756E-836.6324274
ATCAACG104200.035.8194434
TCAACGC104400.035.7508285
GGAACGC2001.8189894E-1235.6962978
CATGGGT7850.035.6407854
CAACGCA105350.035.6341746
Produced by FastQC (version 0.11.5)