##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936209_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 878889 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.478616753651487 31.0 31.0 34.0 30.0 34.0 2 31.37587795500911 31.0 31.0 34.0 30.0 34.0 3 31.718666407248243 31.0 31.0 34.0 30.0 34.0 4 35.565346704760216 37.0 35.0 37.0 33.0 37.0 5 35.205967989131736 37.0 35.0 37.0 33.0 37.0 6 35.41464621812311 37.0 35.0 37.0 33.0 37.0 7 35.249292003882175 37.0 35.0 37.0 32.0 37.0 8 35.332865697488536 37.0 35.0 37.0 32.0 37.0 9 36.98959368020307 39.0 37.0 39.0 33.0 39.0 10-11 36.87400058482926 39.0 37.0 39.0 32.0 39.0 12-13 36.809957798993956 39.0 37.0 39.0 32.0 39.0 14-15 37.85255931067518 40.0 37.0 41.0 32.0 41.0 16-17 37.79403997546903 40.0 37.0 41.0 32.0 41.0 18-19 38.00249121333866 40.0 37.0 41.0 33.0 41.0 20-21 38.079470786413296 40.0 37.0 41.0 33.0 41.0 22-23 37.94661384998561 40.0 37.0 41.0 32.5 41.0 24-25 37.87924299883148 40.0 37.0 41.0 33.0 41.0 26-27 37.66125927164864 40.0 36.5 41.0 32.0 41.0 28-29 37.47637244293648 40.0 36.0 41.0 32.0 41.0 30-31 37.159575896387366 39.0 36.0 41.0 31.5 41.0 32-33 36.76009655371725 39.0 35.0 41.0 31.0 41.0 34-35 36.42790898509368 39.0 35.0 41.0 30.0 41.0 36-37 36.10693159204405 39.0 35.0 41.0 30.0 41.0 38-39 35.98640670209777 39.0 35.0 41.0 30.0 41.0 40-41 35.88254660144796 39.0 35.0 41.0 29.5 41.0 42-43 35.654683355918664 39.0 35.0 41.0 28.5 41.0 44-45 35.27208612236585 39.0 35.0 41.0 26.0 41.0 46-47 34.64983291405399 39.0 35.0 41.0 21.5 41.0 48-49 34.12117969390901 39.0 34.0 41.0 15.5 41.0 50-51 33.86169698335057 39.0 34.0 41.0 11.0 41.0 52-53 33.70642538477556 38.5 34.0 41.0 8.5 41.0 54-55 33.52350126125142 38.0 33.0 40.5 7.5 41.0 56-57 33.329071703025065 38.0 33.0 40.0 7.0 41.0 58-59 33.116188164830824 38.0 33.0 40.0 5.5 41.0 60-61 32.89500778824174 37.5 33.0 40.0 2.0 41.0 62-63 32.68075661431648 37.0 33.0 40.0 2.0 41.0 64-65 32.44865847678148 37.0 32.0 40.0 2.0 41.0 66-67 32.177449598299674 36.0 32.0 39.5 2.0 41.0 68-69 31.867638575519777 36.0 32.0 39.0 2.0 41.0 70-71 31.550769778663742 35.0 31.5 39.0 2.0 41.0 72-73 31.282077714023046 35.0 31.0 38.5 2.0 40.0 74-75 31.08371250521966 35.0 31.0 37.5 2.0 40.0 76-77 30.781614060478628 35.0 31.0 37.0 2.0 39.0 78-79 30.45070594807763 35.0 31.0 36.5 2.0 39.0 80-81 30.17744163369891 35.0 31.0 36.0 2.0 38.5 82-83 29.90955001143489 35.0 31.0 36.0 2.0 37.0 84-85 29.662702002186855 35.0 31.0 35.0 2.0 37.0 86-87 29.458851459057968 35.0 31.0 35.0 2.0 36.5 88-89 29.28691564008652 34.5 30.0 35.0 2.0 36.0 90-91 29.13771932519351 34.0 30.0 35.0 2.0 36.0 92-93 29.007673323935105 34.0 30.0 35.0 2.0 36.0 94-95 28.8872536804989 34.0 30.0 35.0 2.0 35.0 96-97 28.813405333324233 34.0 30.0 35.0 2.0 35.0 98-99 28.730151930448557 34.0 30.0 35.0 2.0 35.0 100-101 28.643296821327837 34.0 30.0 35.0 2.0 35.0 102-103 27.657743469311825 33.5 28.0 34.5 2.0 35.0 104-105 28.366479726108757 34.0 29.0 35.0 2.0 35.0 106-107 28.508490833313424 34.0 30.0 35.0 2.0 35.0 108-109 28.464068841457795 34.0 30.0 35.0 2.0 35.0 110-111 28.424174725135938 34.0 30.0 35.0 2.0 35.0 112-113 28.336586303844967 34.0 29.0 35.0 2.0 35.0 114-115 28.29970053101131 34.0 30.0 35.0 2.0 35.0 116-117 28.202601807509254 34.0 29.0 35.0 2.0 35.0 118-119 28.068837475494632 34.0 29.0 35.0 2.0 35.0 120-121 27.862254505403982 34.0 29.0 35.0 2.0 35.0 122-123 27.636014331730173 34.0 29.0 35.0 2.0 35.0 124-125 25.644583673251116 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24204886899860156 1101 2 0.579352653389968 1101 3 0.42582004000892937 1101 4 0.20863230474979133 1101 5 0.423027971759268 1101 6 0.30809708162077953 1101 7 0.46396247048964767 1101 8 0.42306988708894977 1101 9 0.3391979663447131 1101 10-11 0.37871690402477043 1101 12-13 0.46847057797533154 1101 14-15 0.4880313285775557 1101 16-17 0.6065535000288662 1101 18-19 0.43800781795430765 1101 20-21 0.5957445961534873 1101 22-23 0.43980316415314036 1101 24-25 0.38908863901176005 1101 26-27 0.38958980126580656 1101 28-29 0.48056886930782383 1101 30-31 0.18042271094614648 1101 32-33 0.24010585343567215 1101 34-35 0.2768928745751822 1101 36-37 0.39366551648647174 1101 38-39 0.47845761479035787 1101 40-41 0.34504944418837624 1101 42-43 0.19362365405481796 1101 44-45 0.10302896405875828 1101 46-47 0.009379469560194309 1101 48-49 0.007895803754863095 1101 50-51 -0.07412890144123452 1101 52-53 -0.15362199748170724 1101 54-55 -0.12809017317334082 1101 56-57 -0.1315183295657576 1101 58-59 0.03273787306774523 1101 60-61 -0.03393396038671881 1101 62-63 -0.04931012044661287 1101 64-65 0.06543589765414026 1101 66-67 -0.05434928991388688 1101 68-69 0.010864537317939238 1101 70-71 0.04889219737930972 1101 72-73 0.3016920477665046 1101 74-75 0.11158668386697812 1101 76-77 0.07051935205625526 1101 78-79 0.1797401405579464 1101 80-81 0.1292757347988136 1101 82-83 0.16830016871423936 1101 84-85 0.07407253287044213 1101 86-87 0.07567511583786413 1101 88-89 0.08458639157926129 1101 90-91 0.21448761873977773 1101 92-93 0.11712559921760146 1101 94-95 0.08947957693759179 1101 96-97 0.10957702984932638 1101 98-99 0.15305459578910785 1101 100-101 0.24216713765704512 1101 102-103 0.32257225657467004 1101 104-105 0.14980594412114812 1101 106-107 0.05728488870055415 1101 108-109 0.11381503984263475 1101 110-111 0.041673512055233175 1101 112-113 0.012724946059261555 1101 114-115 0.0020093359178225967 1101 116-117 -0.08017012107869093 1101 118-119 0.04288640172252656 1101 120-121 -0.009759470422448402 1101 122-123 0.08898711773849044 1101 124-125 -0.03587776942948295 1102 1 0.32697876803528914 1102 2 0.6419168655916181 1102 3 0.48027738583081003 1102 4 0.1556310203707909 1102 5 0.4510721362569612 1102 6 0.35126981628989284 1102 7 0.42820231409665865 1102 8 0.5120197466386784 1102 9 0.3372862445911977 1102 10-11 0.5358049862941243 1102 12-13 0.45549343711420676 1102 14-15 0.5138986893163775 1102 16-17 0.5533891713207026 1102 18-19 0.48822142222196874 1102 20-21 0.46120765014652676 1102 22-23 0.5163905383674106 1102 24-25 0.39716095295091236 1102 26-27 0.4588890399783594 1102 28-29 0.5129034751427568 1102 30-31 0.18337370532759678 1102 32-33 0.1682227400232179 1102 34-35 0.15748638832624096 1102 36-37 0.3130149912083837 1102 38-39 0.2824049072017232 1102 40-41 0.11088107554407145 1102 42-43 0.15166673338991643 1102 44-45 0.17856194895232846 1102 46-47 -0.06071042617241318 1102 48-49 -0.007597726570573116 1102 50-51 -0.10877312197866473 1102 52-53 -0.1121728357293108 1102 54-55 0.002231645019271866 1102 56-57 -0.09445084918911562 1102 58-59 -0.07869023868995839 1102 60-61 -0.04046356184051092 1102 62-63 0.04552796087796196 1102 64-65 0.1261682906212016 1102 66-67 -0.046152803141261245 1102 68-69 -0.17803149600789325 1102 70-71 -0.22446295797980298 1102 72-73 -0.21378042716543177 1102 74-75 -0.2144666121929113 1102 76-77 -0.11852163955560258 1102 78-79 0.01725323944217294 1102 80-81 -0.08251129043580363 1102 82-83 0.008648187374713956 1102 84-85 -0.07409157178674164 1102 86-87 -0.10972923139905078 1102 88-89 -0.06316389361334274 1102 90-91 0.07122375200854236 1102 92-93 0.07844738123812789 1102 94-95 -0.09470193783060665 1102 96-97 -0.03986686481540147 1102 98-99 -0.006845191335465017 1102 100-101 0.07742865914137909 1102 102-103 0.07293681181727507 1102 104-105 -0.041046621154620766 1102 106-107 -0.016615763314796084 1102 108-109 -0.007115802907588176 1102 110-111 -0.09865879604240035 1102 112-113 -0.145414016298556 1102 114-115 -0.09716156747193239 1102 116-117 -0.05518600710314203 1102 118-119 0.03212547599785509 1102 120-121 -0.06286765141461004 1102 122-123 -0.09924000193226945 1102 124-125 -0.08415805666824383 1103 1 0.17180978389670898 1103 2 0.6519971075130826 1103 3 0.2624100252269592 1103 4 0.21091229102368203 1103 5 0.3962175108786852 1103 6 0.23867133460357337 1103 7 0.362567422448258 1103 8 0.28012200671728493 1103 9 0.3936322261905545 1103 10-11 0.3407713003398385 1103 12-13 0.28259312971221817 1103 14-15 0.4126169056021567 1103 16-17 0.5952550296983858 1103 18-19 0.47362064262963344 1103 20-21 0.27639143914788633 1103 22-23 0.2632716798853778 1103 24-25 0.1556688575736871 1103 26-27 0.3480237707516096 1103 28-29 0.1407997715356757 1103 30-31 0.16282788199460896 1103 32-33 0.20865458113073743 1103 34-35 0.3254283710908652 1103 36-37 0.2270079235712572 1103 38-39 0.2275073455474086 1103 40-41 0.19406304491691628 1103 42-43 0.3734018915693298 1103 44-45 0.3493021268715566 1103 46-47 0.43121157546242017 1103 48-49 0.5181681152847943 1103 50-51 0.4835743835867419 1103 52-53 0.4036605954335073 1103 54-55 0.4448174197418666 1103 56-57 0.4225084363512579 1103 58-59 0.3413039893701466 1103 60-61 0.2719603485563695 1103 62-63 0.19659761764862793 1103 64-65 0.3373721042321165 1103 66-67 0.24108463337826436 1103 68-69 0.28669173349574706 1103 70-71 0.2060726693463657 1103 72-73 0.3342272209266781 1103 74-75 0.28433606355233465 1103 76-77 0.35142867393356525 1103 78-79 0.30932073526811266 1103 80-81 0.3069835072093312 1103 82-83 0.2759346582084845 1103 84-85 0.11106704242592613 1103 86-87 0.06767041728438272 1103 88-89 0.28161630526313886 1103 90-91 0.39843730726263615 1103 92-93 0.25489319239677855 1103 94-95 0.3010588386311852 1103 96-97 0.2487510545035292 1103 98-99 0.20526001232379443 1103 100-101 0.35320428394571834 1103 102-103 0.3065174907104513 1103 104-105 0.240742256284598 1103 106-107 0.15975558873223505 1103 108-109 0.2346757594076223 1103 110-111 0.21827016235615204 1103 112-113 0.18806116979487442 1103 114-115 0.1681126051079822 1103 116-117 0.12784563095383206 1103 118-119 0.1839106467864049 1103 120-121 0.09498131724517123 1103 122-123 0.1824649051949372 1103 124-125 0.16349729656143452 1104 1 0.1315048647592434 1104 2 0.46833397426268064 1104 3 0.26048997200973645 1104 4 0.1298057010727547 1104 5 0.40156623055523255 1104 6 0.1922855270149313 1104 7 0.3001883706824131 1104 8 0.34026459604619674 1104 9 0.5048171301231008 1104 10-11 0.2669988169270212 1104 12-13 0.315026154627553 1104 14-15 0.3507086880335706 1104 16-17 0.6231740937318335 1104 18-19 0.4451767496515444 1104 20-21 0.3425004480634968 1104 22-23 0.30054803364168237 1104 24-25 0.23833913540905627 1104 26-27 0.11327296730976855 1104 28-29 0.1895062733671793 1104 30-31 0.17584169601077804 1104 32-33 0.1511091391513304 1104 34-35 0.09786588836917787 1104 36-37 -0.003956228236056347 1104 38-39 0.0411433950325133 1104 40-41 0.04196470104380978 1104 42-43 -0.016507678027736006 1104 44-45 -0.05886252687092508 1104 46-47 -0.09322968689158273 1104 48-49 -0.24792177949024108 1104 50-51 -0.17934018808372088 1104 52-53 -0.15271046079014639 1104 54-55 -0.09512735443049536 1104 56-57 -0.24172630689292163 1104 58-59 -0.26135352410694423 1104 60-61 -0.13194627940686132 1104 62-63 -0.07809580017568862 1104 64-65 -0.06616034013169525 1104 66-67 -0.044437970982784236 1104 68-69 -0.04378743749050429 1104 70-71 -0.1125546545875622 1104 72-73 -0.09265417205141091 1104 74-75 -0.05825520916865656 1104 76-77 -0.0795816973775132 1104 78-79 -0.08593539421560692 1104 80-81 -0.04545262780345638 1104 82-83 -0.08440252819002225 1104 84-85 0.028312532784621425 1104 86-87 0.11880992243332145 1104 88-89 0.07364419678985357 1104 90-91 -0.07568409020781885 1104 92-93 0.097089223566865 1104 94-95 0.04593311726135951 1104 96-97 -0.0030053946290991007 1104 98-99 -0.04329292844612809 1104 100-101 -0.10490173150109783 1104 102-103 -0.10970155950034055 1104 104-105 -0.09583670131440769 1104 106-107 0.016725662683438003 1104 108-109 -0.0022888286772477784 1104 110-111 0.05258727998694823 1104 112-113 0.039470864659758575 1104 114-115 0.1054144443850582 1104 116-117 0.10316085228910055 1104 118-119 -0.02189465247408151 1104 120-121 -0.005025594082496809 1104 122-123 -0.06109784102111249 1104 124-125 0.04572179376234686 1105 1 0.2577995255380472 1105 2 0.5448257549294908 1105 3 0.24162101813476156 1105 4 0.17831983914931726 1105 5 0.3331064419830412 1105 6 0.339741496710964 1105 7 0.35857806074612597 1105 8 0.16735288311039653 1105 9 0.16614045820676893 1105 10-11 0.06074502121116154 1105 12-13 0.11635864389661066 1105 14-15 0.009729367608237283 1105 16-17 0.22487920649676596 1105 18-19 0.08365578700652776 1105 20-21 0.19799098322083353 1105 22-23 0.28505327816299086 1105 24-25 0.2967972770265703 1105 26-27 0.2838995306300234 1105 28-29 0.34968085056303266 1105 30-31 0.3277753237372565 1105 32-33 0.3287913592462317 1105 34-35 0.17786156511314744 1105 36-37 0.19136487645878475 1105 38-39 0.20759853095773906 1105 40-41 0.3703659197902738 1105 42-43 0.3911846296645649 1105 44-45 0.4567331045817937 1105 46-47 0.4917897162932192 1105 48-49 0.35120224649047316 1105 50-51 0.33733419868298853 1105 52-53 0.17952970637576016 1105 54-55 0.23591259451390556 1105 56-57 0.19942079704021154 1105 58-59 0.2424200607987146 1105 60-61 0.1898681499748136 1105 62-63 0.1943069008299858 1105 64-65 0.3111420510406262 1105 66-67 0.27409026914321544 1105 68-69 0.3051852745291832 1105 70-71 0.2851190219299511 1105 72-73 0.26851990076457 1105 74-75 0.33089444754422814 1105 76-77 0.4230487980470379 1105 78-79 0.4786833872437981 1105 80-81 0.3302342037645474 1105 82-83 0.1762069479754551 1105 84-85 0.17639125721822424 1105 86-87 0.13440040715267187 1105 88-89 0.29053062897134296 1105 90-91 0.2349747945979992 1105 92-93 0.16605390668249242 1105 94-95 0.18430510256228771 1105 96-97 0.31892740962004495 1105 98-99 0.2832868613612831 1105 100-101 0.21428426114936272 1105 102-103 0.3408877414703291 1105 104-105 0.3671464482805433 1105 106-107 0.3695326910625276 1105 108-109 0.3552336368139031 1105 110-111 0.3723893366216018 1105 112-113 0.4285911849924453 1105 114-115 0.4934719642771803 1105 116-117 0.4414870316630548 1105 118-119 0.39875043908630303 1105 120-121 0.42755477215904847 1105 122-123 0.42750986466808527 1105 124-125 0.5944433451936533 1106 1 0.08971441915506873 1106 2 0.5923434854259462 1106 3 0.2699898124610023 1106 4 0.07761061929116408 1106 5 0.37069509446531157 1106 6 0.12626631940599964 1106 7 0.28410997563974405 1106 8 0.14678550722387484 1106 9 -0.0884694708712459 1106 10-11 0.12918473752321802 1106 12-13 0.09437282829377835 1106 14-15 0.13242440306922987 1106 16-17 0.14863807174499044 1106 18-19 0.20174089338951262 1106 20-21 0.12813282719246644 1106 22-23 0.018032001567235056 1106 24-25 0.2719745819911097 1106 26-27 0.24028250935342754 1106 28-29 0.08726950304529879 1106 30-31 0.038059011680523724 1106 32-33 0.02418143016821972 1106 34-35 -0.008663257581886796 1106 36-37 -0.013245052619218711 1106 38-39 -0.048784447765662264 1106 40-41 -0.07289649155724476 1106 42-43 -0.01555295898791087 1106 44-45 -0.1007137039288395 1106 46-47 -0.1372882978911747 1106 48-49 -0.1817764769137895 1106 50-51 -0.061247361600699435 1106 52-53 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-0.4957600701793119 2216 118-119 -0.6189926143764168 2216 120-121 -0.4596355720034495 2216 122-123 -0.5539082736800118 2216 124-125 -0.684653755644149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56.0 3 49.0 4 148.0 5 312.0 6 774.0 7 1536.0 8 2577.0 9 4428.0 10 7889.0 11 10335.0 12 10877.0 13 13607.0 14 19563.0 15 14587.0 16 5929.0 17 4614.0 18 4370.0 19 3912.0 20 3880.0 21 3771.0 22 4119.0 23 4848.0 24 5744.0 25 7063.0 26 8738.0 27 10747.0 28 13715.0 29 16988.0 30 21315.0 31 26424.0 32 33442.0 33 41816.0 34 57035.0 35 80004.0 36 130517.0 37 213500.0 38 89282.0 39 378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.01802188493728 17.56886149824495 11.625704042144045 19.787412574673723 2 19.422310870299846 22.203233429781736 37.0361857862441 21.33826991367432 3 17.63556034948668 29.655622041008588 33.17290351796416 19.535914091540572 4 10.742994849178906 17.055623633928743 36.63739106986206 35.563990447030285 5 9.608178685002443 43.45569708169235 34.407588442449644 12.528535790855566 6 27.926766550459213 37.63847043081712 19.092648892691674 15.342114126031985 7 25.433587176537653 36.056885454249624 22.30429553675151 16.20523183246121 8 28.835268162418693 31.923598998280784 20.42829071703025 18.812842122270276 9 28.374345338262287 10.610782476512961 20.296533464407908 40.718338720816845 10-11 24.32178580002708 26.35105229443081 29.20658922799125 20.120572677550864 12-13 26.263783026070413 26.35947201523742 29.608289556474137 17.768455402218027 14-15 22.997955373204125 26.536399932187116 29.649477920419987 20.816166774188776 16-17 19.885446285025754 28.41149451182117 30.215590364653554 21.487468838499517 18-19 19.016792791808747 25.151469639510793 35.73733429363662 20.094403275043835 20-21 20.706653513697407 24.063334505267445 36.495962516313206 18.734049464721938 22-23 20.16096458142041 24.798865385731304 35.816411401212214 19.223758631636077 24-25 19.453480473643427 24.927607468064792 36.3510636724319 19.267848385859875 26-27 19.52464987046146 24.7501106510606 36.22124067999486 19.503998798483085 28-29 19.2912870681053 24.825546798287384 36.38661992583819 19.49654620776913 30-31 19.561287033971297 24.473966564606 36.60627223688088 19.358474164541825 32-33 19.221198581390826 24.865426692107878 36.22300427016381 19.69037045633749 34-35 19.67945785915352 24.99442476043537 35.96023830166825 19.36587907874286 36-37 20.13718455914228 24.688498775158184 35.08162009081921 20.092696574880332 38-39 19.978916563980206 25.12905497736347 34.952422888442115 19.939605570214212 40-41 20.08086345374672 25.144699728862236 34.83744818742754 19.936988629963512 42-43 19.94839950276919 25.481144768693802 34.152759090534126 20.417696638002887 44-45 20.279244424214838 25.54056668183887 33.50421028675466 20.675978607191624 46-47 20.652970468283446 25.917404049231667 32.0839364394436 21.345689043041293 48-49 20.803785599519347 26.3839263362479 31.25378355128106 21.558504512951693 50-51 21.137944022220882 26.623468246373232 30.76445159550439 21.474136135901496 52-53 21.05559904782278 26.887456931276652 30.448790889684897 21.608153131215666 54-55 20.808456310315044 27.095786068411954 30.541929375994936 21.55382824527806 56-57 21.18675021647928 27.109236411302668 30.071560482774395 21.632452889443663 58-59 21.32693332847852 26.358374859973814 30.48898365882368 21.825708152723973 60-61 21.478448957578337 26.63508813051349 30.685530347595257 21.200932564312915 62-63 21.273377173444928 26.514108084705967 30.91484516293832 21.297669578910778 64-65 21.127434442919128 26.421951331906886 31.145451866399128 21.305162358774858 66-67 21.416099979007004 26.726588683465994 30.512951655232406 21.3443596822946 68-69 21.470056976085974 26.722097220602414 30.47694360135514 21.330902201956473 70-71 21.22991795654274 26.425662233099906 30.809619468779854 21.534800341577505 72-73 21.499031423963224 26.779121995067516 30.131902704332564 21.589943876636696 74-75 21.00616693974149 27.08833970507919 30.240305843801202 21.665187511378118 76-77 20.73956584541357 27.20887538180674 30.398956399647055 21.652602373132638 78-79 20.980944102092643 27.03040660470959 30.00604172545307 21.982607567744697 80-81 20.635670142857386 26.988787478659006 30.10353476882886 22.272007609654747 82-83 20.88728180487469 26.965256605944 29.753785045054165 22.393676544127143 84-85 20.762388601434207 26.67870847354453 29.92299880814557 22.63590411687569 86-87 20.644407769166484 26.879299839740035 29.78195655970722 22.694335831386265 88-89 21.138180392808316 26.464013999691648 29.900389872752314 22.497415734747715 90-91 20.93118118317436 26.712790130087054 29.695758090190505 22.66027059654808 92-93 20.686885190876346 26.747492975438643 29.708191272874977 22.85743056081003 94-95 20.4209799312305 26.666670459381166 29.853972906364707 23.05837670302363 96-97 20.74500040961596 26.804813442685628 29.56147197771689 22.888714169981522 98-99 20.61661888770113 26.711580221727015 29.284476093544747 23.387324797027105 100-101 20.481478068034548 26.7976633817929 29.527283112426428 23.193575437746127 102-103 20.882719385578156 26.311648414165838 29.385635044801596 23.419997155454418 104-105 20.377821988111 26.358789799966893 29.672156421898567 23.59123179002355 106-107 20.291119854542476 26.64720633714196 29.33078615786875 23.730887650446817 108-109 20.519365851632678 26.791670530487966 28.794561711816012 23.894401906063344 110-111 20.390577087403386 26.82758377433071 29.007804259744514 23.774034878521388 112-113 20.26834502738981 26.348381987132363 29.05442003472941 24.328852950748413 114-115 19.90635076979804 26.822782858173593 28.843428023869226 24.427438348159136 116-117 20.068759182400743 26.58824948589936 28.924889526686005 24.41810180501389 118-119 19.888220541111355 26.372635368873354 28.869949839778602 24.86919425023669 120-121 19.72032197280026 26.73394915069092 28.719766690334442 24.825962186174376 122-123 19.81379619536152 26.43845966532472 28.587757247770313 25.15998689154345 124-125 19.480314073632414 26.42061084033073 28.47724581933996 25.621829266696892 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 129.0 1 106.0 2 328.5 3 635.0 4 524.0 5 476.5 6 750.5 7 1696.0 8 2669.5 9 3103.0 10 2822.5 11 2177.0 12 2062.0 13 2134.5 14 2336.5 15 2523.0 16 2677.5 17 2942.0 18 3148.5 19 3245.0 20 3434.5 21 3847.5 22 4364.5 23 4923.5 24 5634.5 25 6548.0 26 7808.5 27 9300.5 28 10279.0 29 11504.5 30 13886.5 31 15843.0 32 17849.0 33 20274.5 34 22618.0 35 24213.0 36 25932.5 37 28558.5 38 28580.5 39 27387.5 40 27333.5 41 27509.0 42 27286.0 43 27795.5 44 29370.5 45 30575.5 46 30050.5 47 28943.5 48 28257.0 49 27473.5 50 27503.0 51 25696.0 52 22903.0 53 22080.0 54 21224.0 55 19873.0 56 18286.0 57 16235.5 58 15182.0 59 13922.0 60 12539.0 61 11557.5 62 9637.5 63 6711.5 64 4551.0 65 3683.5 66 2768.5 67 2121.5 68 1768.5 69 1309.5 70 949.5 71 850.5 72 839.5 73 836.5 74 702.0 75 556.0 76 358.5 77 186.5 78 115.5 79 61.0 80 33.0 81 18.0 82 6.5 83 6.0 84 5.0 85 2.5 86 2.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06462704619127102 2 9.102400872010003E-4 3 0.0 4 0.0 5 0.06895068660547578 6 1.1378001090012504E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.4134003270037516E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0024462702343526883 44-45 0.01012642097011113 46-47 0.01194690114451313 48-49 0.009671300926510628 50-51 0.008647280828409503 52-53 0.006940580664907627 54-55 0.007509480719408252 56-57 0.005916560566806502 58-59 0.004039190386954439 60-61 0.002503160239802751 62-63 0.001536030147151688 64-65 0.0019911501907521885 66-67 0.0027876102670530635 68-69 0.0013084701253514382 70-71 0.002901390277953189 72-73 0.0024462702343526883 74-75 0.0010240200981011254 76-77 9.671300926510628E-4 78-79 1.1378001090012504E-4 80-81 6.257900599506877E-4 82-83 2.8445002725031263E-4 84-85 7.395700708508128E-4 86-87 5.120100490505627E-4 88-89 0.0014222501362515629 90-91 0.0018204801744020006 92-93 9.671300926510628E-4 94-95 0.0012515801199013755 96-97 0.0019342601853021256 98-99 0.0017635901689519382 100-101 0.0013653601308015006 102-103 0.0015929201526017505 104-105 0.0010809101035511878 106-107 7.964600763008753E-4 108-109 0.0010809101035511878 110-111 0.0011378001090012505 112-113 0.010809101035511878 114-115 0.02099241201107307 116-117 0.019342601853021255 118-119 0.01194690114451313 120-121 0.0064854606213071275 122-123 0.007623260730308379 124-125 0.005745890550456315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 878889.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.244736563653795 #Duplication Level Percentage of deduplicated Percentage of total 1 47.6924852419984 13.947541669681893 2 15.765746818806708 9.221302248905301 3 11.09810817716431 9.736837499883068 4 7.7309543259299796 9.043588905898476 5 5.186254597930121 7.5835324734252305 6 3.416832859937019 5.995462612253634 7 2.2606589911646138 4.6278663659805135 8 1.508314818778997 3.528821562419758 9 1.036970551393836 2.729333753980159 >10 3.948171029539236 19.299494417754747 >50 0.24131815167692441 4.869180635188962 >100 0.10712148089559548 5.594407163801975 >500 0.003923863769018858 0.7832996246271829 >1k 0.001961931884509429 0.7362511561101678 >5k 0.0011771591307056573 2.3030799100890067 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8209 0.9340201094791265 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5942 0.676080824768543 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5919 0.6734638845178401 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1450 0.16498101580518132 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1448 0.16475345578338105 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1216 0.13835649325455204 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1177 0.13391907282944718 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1125 0.12800251226264067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 909 0.10342602990821366 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.826800654007503E-4 10-11 0.0 0.0 0.0 0.0 6.826800654007503E-4 12-13 0.0 0.0 0.0 0.0 6.826800654007503E-4 14-15 0.0 0.0 0.0 0.0 6.826800654007503E-4 16-17 0.0 0.0 0.0 0.0 6.826800654007503E-4 18-19 0.0 0.0 0.0 0.0 6.826800654007503E-4 20-21 0.0 0.0 0.0 1.1378001090012504E-4 6.826800654007503E-4 22-23 0.0 0.0 0.0 2.8445002725031263E-4 6.826800654007503E-4 24-25 0.0 0.0 0.0 5.120100490505627E-4 6.826800654007503E-4 26-27 0.0 0.0 0.0 0.0012515801199013757 6.826800654007503E-4 28-29 1.1378001090012504E-4 0.0 0.0 0.0017635901689519382 6.826800654007503E-4 30-31 2.2756002180025008E-4 0.0 0.0 0.0052907705068558145 6.826800654007503E-4 32-33 2.2756002180025008E-4 0.0 0.0 0.013653601308015004 6.826800654007503E-4 34-35 2.2756002180025008E-4 0.0 0.0 0.02577117246887832 6.826800654007503E-4 36-37 2.2756002180025008E-4 0.0 0.0 0.045967124403650514 6.826800654007503E-4 38-39 2.2756002180025008E-4 0.0 0.0 0.07651705733033409 6.826800654007503E-4 40-41 2.2756002180025008E-4 0.0 0.0 0.11048039058402143 6.826800654007503E-4 42-43 2.2756002180025008E-4 0.0 0.0 0.15092918445901587 6.826800654007503E-4 44-45 2.2756002180025008E-4 0.0 0.0 0.2009923892550709 6.826800654007503E-4 46-47 2.2756002180025008E-4 0.0 0.0 0.26311627520653913 6.826800654007503E-4 48-49 2.2756002180025008E-4 0.0 0.0 0.32905179152316166 0.0011378001090012505 50-51 2.2756002180025008E-4 0.0 0.0 0.39413395775803317 0.0011378001090012505 52-53 2.2756002180025008E-4 0.0 0.0 0.4655878046033117 0.0011378001090012505 54-55 2.2756002180025008E-4 0.0 0.0 0.5539948730727089 0.0011378001090012505 56-57 2.2756002180025008E-4 0.0 0.0 0.6680024439946342 0.0011378001090012505 58-59 2.2756002180025008E-4 0.0 0.0 0.8020921868404315 0.0011378001090012505 60-61 2.2756002180025008E-4 0.0 0.0 0.929070679004971 0.0012515801199013755 62-63 2.2756002180025008E-4 0.0 0.0 1.0582109913766131 0.0012515801199013755 64-65 2.2756002180025008E-4 0.0 0.0 1.2077748157048274 0.0012515801199013755 66-67 2.2756002180025008E-4 0.0 0.0 1.398527003978887 0.0012515801199013755 68-69 2.2756002180025008E-4 0.0 0.0 1.6301262161660914 0.0012515801199013755 70-71 2.2756002180025008E-4 0.0 0.0 1.8609289682769952 0.0012515801199013755 72-73 2.2756002180025008E-4 0.0 0.0 2.0757456288564313 0.0015929201526017505 74-75 2.2756002180025008E-4 0.0 0.0 2.317528152019197 0.0015929201526017505 76-77 2.2756002180025008E-4 0.0 0.0 2.6043106694929623 0.0015929201526017505 78-79 2.2756002180025008E-4 0.0 0.0 2.9617505737357046 0.0015929201526017505 80-81 2.2756002180025008E-4 0.0 0.0 3.3307960390902602 0.0015929201526017505 82-83 2.2756002180025008E-4 0.0 0.0 3.694892073970661 0.0015929201526017505 84-85 2.2756002180025008E-4 0.0 0.0 4.069512759859322 0.0015929201526017505 86-87 2.2756002180025008E-4 0.0 0.0 4.49556201067484 0.0015929201526017505 88-89 2.2756002180025008E-4 0.0 0.0 4.996478508662641 0.0015929201526017505 90-91 2.2756002180025008E-4 0.0 0.0 5.502799557168197 0.0015929201526017505 92-93 2.2756002180025008E-4 0.0 0.0 6.004796965259549 0.0015929201526017505 94-95 2.2756002180025008E-4 0.0 0.0 6.558962508348609 0.0015929201526017505 96-97 2.2756002180025008E-4 0.0 0.0 7.165068626413575 0.0015929201526017505 98-99 2.2756002180025008E-4 0.0 0.0 7.826528719781451 0.0015929201526017505 100-101 2.2756002180025008E-4 0.0 0.0 8.554607009531352 0.0015929201526017505 102-103 2.2756002180025008E-4 0.0 0.0 9.249347756087516 0.0015929201526017505 104-105 2.2756002180025008E-4 0.0 0.0 9.934132751689917 0.0015929201526017505 106-107 2.2756002180025008E-4 0.0 0.0 10.67290636246443 0.0015929201526017505 108-109 6.257900599506877E-4 0.0 0.0 11.484726740236821 0.0015929201526017505 110-111 0.0010240200981011254 0.0 0.0 12.315946609867684 0.0015929201526017505 112-113 0.0010240200981011254 0.0 0.0 13.135845368413985 0.0015929201526017505 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4545 0.0 78.90026 1 GTATCAA 7165 0.0 50.097977 2 GTACATG 9920 0.0 47.258385 1 TACATGG 10010 0.0 46.62309 2 ACATGGG 10120 0.0 46.29278 3 CATGGGA 7630 0.0 44.314194 4 TACCTGG 815 0.0 43.82407 2 ATGGGAT 2900 0.0 43.516796 5 ATCACGC 100 2.2166387E-7 41.669384 3 ATGGGAG 2060 0.0 41.611588 5 CACGCAG 230 0.0 41.410572 5 TAACGCA 105 3.2506796E-7 39.68513 4 CATGGGG 2250 0.0 39.155994 4 GTATCGA 65 0.0019342272 36.632427 2 CCACGCA 130 4.1836756E-8 36.632427 4 ATCAACG 10420 0.0 35.819443 4 TCAACGC 10440 0.0 35.750828 5 GGAACGC 200 1.8189894E-12 35.696297 8 CATGGGT 785 0.0 35.640785 4 CAACGCA 10535 0.0 35.634174 6 >>END_MODULE