FastQCFastQC Report
Thu 26 May 2016
SRR936208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210844
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.059.700091
GTACATG268100.051.482331
TACATGG270800.049.8705562
ACATGGG272850.047.9259533
GTATCAA76700.044.755612
CATGGGA166300.042.750654
CATGGGG105900.040.280074
TCAACGC87950.039.2336925
ATCAACG88750.038.6118974
CAACGCA90350.037.8627246
AACGCAG93850.036.2605137
ATGGGAT71500.034.198175
ATGGGAG75150.032.5371785
TATCAAC106750.032.3241543
ACGCAGA111650.030.054048
ATGGGGG60750.029.6731325
CGCAGAG112550.029.1793829
GAGTACA169150.028.5595321
AGTACAT166950.027.1541122
TACCTGG36950.025.2785572
Produced by FastQC (version 0.11.5)