FastQCFastQC Report
Thu 26 May 2016
SRR936208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210844
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120110.2852397286624724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91650.21765232813184243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89860.21340139886445567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90400.075.512221
GTATCAA136750.049.58672
GTACATG248350.044.7317351
TACATGG254650.043.274452
ACATGGG253150.042.7547843
CATGGGA154900.037.5886084
TCAACGC192100.036.538885
ATCAACG192900.036.3873444
CAACGCA196000.036.054834
AACGCAG200550.035.8008585
CATGGGG101600.034.3378144
TATCAAC209700.033.898053
ACGCAGA232050.031.279016
CGCAGAG234050.030.9608887
ATGGGAG67650.030.097275
GAGTACA169500.029.1330471
ATGGGAT71300.028.8070245
AGTACAT165300.028.6327512
CAGAGTA263450.027.4606069
GCAGAGT265950.026.9787658
Produced by FastQC (version 0.11.5)