FastQCFastQC Report
Thu 26 May 2016
SRR936207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406381
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25220.17932551705405578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.060.9005241
GTACATG127250.056.430841
TACATGG131000.053.2713552
ACATGGG129300.051.349083
CATGGGA89900.049.1695374
ATGGGAT35200.045.464945
GTCGGGA4050.044.0690082
ATGGGAG31150.043.163575
CATGGGG33250.040.9742364
GTATCAA34550.040.121292
CATGGGT12150.035.2552074
CGGGAGT5400.035.2552034
TCAACGC39600.034.8545765
CAACGCA39750.034.573386
GGGATAC3300.034.2548527
ATCAACG40950.033.996094
AACGCAG41050.033.4796757
ATGGGAC16850.033.189065
GAGTACA72200.031.3121871
ATGGGTA7750.030.7061465
Produced by FastQC (version 0.11.5)