##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936207_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1406381 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.057938069413623 31.0 30.0 31.0 23.0 34.0 2 29.344146429737034 31.0 30.0 34.0 23.0 34.0 3 29.404355576476075 31.0 30.0 34.0 23.0 34.0 4 32.758719009998 35.0 35.0 37.0 23.0 37.0 5 33.12876667133586 35.0 35.0 37.0 27.0 37.0 6 33.00946614039866 36.0 35.0 37.0 26.0 37.0 7 32.952212096153175 37.0 35.0 37.0 25.0 37.0 8 32.7901614142967 37.0 35.0 37.0 25.0 37.0 9 34.088674406153096 39.0 35.0 39.0 19.0 39.0 10-11 34.027022904888504 38.0 34.5 39.0 22.0 39.0 12-13 34.04085450528697 38.0 35.0 39.0 22.0 39.0 14-15 35.123339976862596 39.0 35.0 41.0 22.5 41.0 16-17 35.16211325380533 39.0 35.0 41.0 24.0 41.0 18-19 35.21172285461763 39.0 35.0 41.0 24.0 41.0 20-21 35.323313881515745 39.0 35.0 41.0 24.5 41.0 22-23 35.233412211911286 39.0 34.5 41.0 24.5 41.0 24-25 35.225547700089805 39.0 34.0 41.0 25.5 41.0 26-27 35.316666678517414 39.0 34.0 41.0 26.0 41.0 28-29 35.21247656218336 39.0 34.0 41.0 26.0 41.0 30-31 35.13391819144314 39.0 34.0 41.0 25.5 41.0 32-33 34.955792562612835 39.0 34.0 41.0 25.0 41.0 34-35 34.909670992426655 39.0 34.0 41.0 25.0 41.0 36-37 34.78554566650146 39.0 34.0 41.0 24.5 41.0 38-39 34.53561908188464 38.5 33.0 40.0 24.0 41.0 40-41 34.63580032722285 38.5 33.5 40.5 24.0 41.0 42-43 34.54106924083872 39.0 33.0 41.0 23.5 41.0 44-45 34.436335175176566 39.0 33.0 40.5 23.5 41.0 46-47 34.41289238122529 38.0 33.0 40.0 24.0 41.0 48-49 34.31642670087267 38.0 33.0 40.0 23.0 41.0 50-51 34.1886053636959 38.0 33.0 40.0 23.0 41.0 52-53 34.03711085402889 38.0 33.0 40.0 22.5 41.0 54-55 33.86432446115242 38.0 33.0 40.0 22.0 41.0 56-57 33.66950456526361 38.0 32.5 40.0 21.5 41.0 58-59 33.430489675272916 38.0 32.0 40.0 20.0 41.0 60-61 33.18744351637288 37.0 32.0 40.0 18.5 41.0 62-63 32.92563679401243 37.0 31.5 40.0 18.0 41.0 64-65 32.62268012722015 36.0 31.0 40.0 14.5 41.0 66-67 32.30871577474383 36.0 31.0 39.0 12.5 41.0 68-69 32.01023620199647 35.5 31.0 39.0 9.0 41.0 70-71 31.66577940117223 35.0 31.0 39.0 7.5 40.0 72-73 31.338160853993337 35.0 30.5 38.0 6.5 40.0 74-75 30.996391447267847 35.0 30.0 37.0 2.0 39.5 76-77 30.674422862652442 35.0 30.0 37.0 2.0 39.0 78-79 30.340931795864705 35.0 30.0 36.0 2.0 39.0 80-81 30.041081684124002 34.0 30.0 36.0 2.0 38.5 82-83 29.743127929060474 34.0 29.5 35.5 2.0 37.0 84-85 29.751590074097987 34.0 30.0 35.0 2.0 37.0 86-87 29.628411148899197 34.0 30.0 35.0 2.0 36.5 88-89 29.447182520241668 34.0 30.0 35.0 2.0 36.0 90-91 29.273995098056645 34.0 30.0 35.0 2.0 36.0 92-93 29.147490260462845 34.0 30.0 35.0 2.0 36.0 94-95 29.010101458992978 34.0 29.0 35.0 2.0 35.0 96-97 28.895922228755936 34.0 29.0 35.0 2.0 35.0 98-99 28.777318166272153 34.0 29.0 35.0 2.0 35.0 100-101 28.66159276895806 34.0 29.0 35.0 2.0 35.0 102-103 27.70525234627032 32.5 27.5 34.5 2.0 35.0 104-105 28.338675294959188 34.0 29.0 35.0 2.0 35.0 106-107 28.453074593584525 34.0 29.0 35.0 2.0 35.0 108-109 28.384973204273948 34.0 29.0 35.0 2.0 35.0 110-111 28.25586060960721 34.0 29.0 35.0 2.0 35.0 112-113 28.205222482385643 34.0 29.0 35.0 2.0 35.0 114-115 28.080110226176263 34.0 29.0 35.0 2.0 35.0 116-117 27.937563149672812 34.0 28.5 35.0 2.0 35.0 118-119 27.716167951643257 34.0 28.0 35.0 2.0 35.0 120-121 27.487846465502592 34.0 27.0 35.0 2.0 35.0 122-123 27.171259068488553 34.0 27.0 35.0 2.0 35.0 124-125 25.17812207360594 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33107793728405 1101 2 0.39584510748683854 1101 3 0.42717200441947867 1101 4 0.37640806630179924 1101 5 0.1719400956177708 1101 6 0.3045876371054632 1101 7 0.1866767388306556 1101 8 0.36100266397539116 1101 9 0.38039984408788996 1101 10-11 0.41622860430764774 1101 12-13 0.37924085866753643 1101 14-15 0.5095819268567325 1101 16-17 0.4172070359505611 1101 18-19 0.49131796094750513 1101 20-21 0.46963650337131924 1101 22-23 0.43095072830114134 1101 24-25 0.4927585539003587 1101 26-27 0.39202707496122713 1101 28-29 0.4801569678653479 1101 30-31 0.4490221427483547 1101 32-33 0.6124091111572554 1101 34-35 0.575674814456363 1101 36-37 0.5244754498520265 1101 38-39 0.6225636531714116 1101 40-41 0.662287648357605 1101 42-43 0.6260467073262106 1101 44-45 0.6469833608538522 1101 46-47 0.49107902339532217 1101 48-49 0.5681115096841438 1101 50-51 0.5940321960907085 1101 52-53 0.577493756035544 1101 54-55 0.6485111671951316 1101 56-57 0.566223379068731 1101 58-59 0.5492146398187572 1101 60-61 0.4741894582603621 1101 62-63 0.5018765747533109 1101 64-65 0.5800550804046267 1101 66-67 0.6423790886750282 1101 68-69 0.7313675840865308 1101 70-71 0.5952588462145307 1101 72-73 0.5872379018602238 1101 74-75 0.5543400885961987 1101 76-77 0.4983712694073752 1101 78-79 0.49330580719856343 1101 80-81 0.6020999107995841 1101 82-83 0.6604174997418077 1101 84-85 0.353301677895022 1101 86-87 0.5064901266156987 1101 88-89 0.4459333269762418 1101 90-91 0.29854308331998425 1101 92-93 0.4398091833707696 1101 94-95 0.5996052526646807 1101 96-97 0.5874242469612518 1101 98-99 0.5827102323647502 1101 100-101 0.6205438390041067 1101 102-103 0.6564029649997174 1101 104-105 0.6115338809106987 1101 106-107 0.6117424929210422 1101 108-109 0.605828981341439 1101 110-111 0.634605549261579 1101 112-113 0.5605575651028261 1101 114-115 0.4641996242743822 1101 116-117 0.6276005085354335 1101 118-119 0.6489756650950618 1101 120-121 0.7218252856115477 1101 122-123 0.5531673933179242 1101 124-125 0.263109324576849 1102 1 0.34659417294416883 1102 2 0.41073022641872114 1102 3 0.3078885122942623 1102 4 0.500716187372511 1102 5 0.3463340939326329 1102 6 0.32474529476311886 1102 7 0.4130685983160447 1102 8 0.3643591679613891 1102 9 0.511328614585004 1102 10-11 0.5011044873849713 1102 12-13 0.5644331102716578 1102 14-15 0.603814783814407 1102 16-17 0.5882015916573451 1102 18-19 0.6643322522171928 1102 20-21 0.6026931661492085 1102 22-23 0.683351240323951 1102 24-25 0.8161378234576802 1102 26-27 0.584120292279259 1102 28-29 0.6918613881129332 1102 30-31 0.5990792592173406 1102 32-33 0.7631520303354264 1102 34-35 0.7018904635586978 1102 36-37 0.7067264058439946 1102 38-39 0.6879814540658273 1102 40-41 0.4904886011089147 1102 42-43 0.6257971772205622 1102 44-45 0.581461454054967 1102 46-47 0.49626122898220615 1102 48-49 0.4235756518839011 1102 50-51 0.48318573812194643 1102 52-53 0.5697279416600267 1102 54-55 0.4806410038663316 1102 56-57 0.5555579924591001 1102 58-59 0.5497829689392475 1102 60-61 0.49220909660373735 1102 62-63 0.4652090169076999 1102 64-65 0.40123394812125923 1102 66-67 0.4255884671182173 1102 68-69 0.39895621588379626 1102 70-71 0.4071244888355743 1102 72-73 0.34890059872975243 1102 74-75 0.304752056319316 1102 76-77 0.4185046221846136 1102 78-79 0.38775052169723523 1102 80-81 0.43125239961933914 1102 82-83 0.4277237898448618 1102 84-85 0.4682751045590976 1102 86-87 0.43033240414502316 1102 88-89 0.35110297504157373 1102 90-91 0.38009284351183226 1102 92-93 0.5779933657545939 1102 94-95 0.4963208531572576 1102 96-97 0.4719728243155856 1102 98-99 0.44006205361045403 1102 100-101 0.5054513184259726 1102 102-103 0.4802444323663657 1102 104-105 0.40374052100402835 1102 106-107 0.35202013567170454 1102 108-109 0.3433301965854625 1102 110-111 0.4613206636565472 1102 112-113 0.3780327741247973 1102 114-115 0.49453697601461144 1102 116-117 0.5610347071225057 1102 118-119 0.6128934497862311 1102 120-121 0.5367371399118923 1102 122-123 0.6214418278243734 1102 124-125 0.4845352919110901 1103 1 0.16514150017034623 1103 2 0.2682725416869367 1103 3 0.3231868284451309 1103 4 0.3008122404353344 1103 5 0.09609923445891155 1103 6 0.15342707193948257 1103 7 0.14715413097246 1103 8 0.14783232177934025 1103 9 0.20374707494526945 1103 10-11 0.3137486886680563 1103 12-13 0.22417740549852994 1103 14-15 0.2512687083608327 1103 16-17 0.13130540360102572 1103 18-19 0.22701356859845134 1103 20-21 0.1689199884802406 1103 22-23 0.15084064528171837 1103 24-25 0.23141591897856273 1103 26-27 0.1426308248545567 1103 28-29 0.2668065064431744 1103 30-31 0.2506480921077312 1103 32-33 0.4417667163944259 1103 34-35 0.2963998019607814 1103 36-37 0.32888699942596133 1103 38-39 0.4560697912445306 1103 40-41 0.3757792184120916 1103 42-43 0.41513837003086707 1103 44-45 0.5027188324553578 1103 46-47 0.40339570408141867 1103 48-49 0.35773072971608855 1103 50-51 0.2872062564839837 1103 52-53 0.35340867636860196 1103 54-55 0.42089837172036226 1103 56-57 0.31206905559776743 1103 58-59 0.447185784170145 1103 60-61 0.5289112639632165 1103 62-63 0.44150737605529145 1103 64-65 0.45782848980861246 1103 66-67 0.40048993971551283 1103 68-69 0.33582728736220346 1103 70-71 0.2899738250634769 1103 72-73 0.30472622915910463 1103 74-75 0.29591105739991264 1103 76-77 0.26606074508061184 1103 78-79 0.2720343666104803 1103 80-81 0.34109343747917364 1103 82-83 0.28970622689927694 1103 84-85 0.27942902310220674 1103 86-87 0.31581853486155964 1103 88-89 0.3934083053375552 1103 90-91 0.46074425607596226 1103 92-93 0.29507927077888496 1103 94-95 0.37391891002975797 1103 96-97 0.38820141113489726 1103 98-99 0.28317805472025626 1103 100-101 0.2523545400976239 1103 102-103 0.28989752180279993 1103 104-105 0.32453538038039653 1103 106-107 0.3271650873693446 1103 108-109 0.32069049373340164 1103 110-111 0.20859706445742532 1103 112-113 0.3028676017971641 1103 114-115 0.32099813455733184 1103 116-117 0.22594591077122672 1103 118-119 0.22201812568826895 1103 120-121 0.15528357475319154 1103 122-123 0.2403542418714828 1103 124-125 0.3085136937633557 1104 1 -0.1399254743639453 1104 2 -0.05139492574244997 1104 3 -0.11154899052353784 1104 4 -0.12577885652075338 1104 5 -0.1472260266369858 1104 6 -0.1766535414051873 1104 7 -0.3996105332220594 1104 8 -0.3233630356544097 1104 9 -0.2159969555765926 1104 10-11 -0.05717163120091584 1104 12-13 -0.1405503291182768 1104 14-15 -0.1461886377992414 1104 16-17 -0.012617663413074354 1104 18-19 -0.08276742703672113 1104 20-21 -0.06204968147063994 1104 22-23 -0.17617300241256117 1104 24-25 -0.16673820785692328 1104 26-27 -0.17612261008640218 1104 28-29 -0.103255204378506 1104 30-31 -0.15189791338656278 1104 32-33 0.1344023443187936 1104 34-35 0.04152647632413675 1104 36-37 0.029350759360283973 1104 38-39 0.07185289317237675 1104 40-41 -0.04621001672018821 1104 42-43 0.10279588264297956 1104 44-45 0.09113173931288543 1104 46-47 -0.013245825177506276 1104 48-49 -0.07336884712746183 1104 50-51 -0.04588670669748751 1104 52-53 -0.10399644051731372 1104 54-55 -0.09542408408120906 1104 56-57 -0.058897198088544656 1104 58-59 0.008670268231412592 1104 60-61 0.08699826079610773 1104 62-63 0.06569919325363571 1104 64-65 0.08008028465990691 1104 66-67 0.036627880887117215 1104 68-69 -0.07715528524654403 1104 70-71 0.04123564216894593 1104 72-73 -0.04815492158094514 1104 74-75 -0.13357094239898615 1104 76-77 -0.17941833756754733 1104 78-79 -0.2199405412089277 1104 80-81 -0.12758989538977517 1104 82-83 -0.1763272799528366 1104 84-85 -0.14560022311072984 1104 86-87 -0.1715813647503346 1104 88-89 -0.13750868710588193 1104 90-91 -0.12445005305721679 1104 92-93 -0.15984360280842225 1104 94-95 -0.07819947686341777 1104 96-97 -0.13691499828220088 1104 98-99 -0.07188843523750066 1104 100-101 0.032862469183076115 1104 102-103 -0.04479131343269671 1104 104-105 -0.045902785836624105 1104 106-107 0.005238988521387711 1104 108-109 0.007668488709150267 1104 110-111 0.04872840085085883 1104 112-113 -0.1895646251267955 1104 114-115 -0.21489798273280414 1104 116-117 -0.19274335816027133 1104 118-119 -0.1808649633579158 1104 120-121 -0.05849079591312645 1104 122-123 -0.09065615269899752 1104 124-125 0.038212157938410485 1105 1 -0.13865491141018538 1105 2 0.12336936943187382 1105 3 -0.0677740734826564 1105 4 0.032224935101353935 1105 5 0.022597477367028773 1105 6 -0.025482179715353936 1105 7 0.04545962291314254 1105 8 0.021715107444158832 1105 9 0.13407542379543003 1105 10-11 0.12780326152144994 1105 12-13 0.22124371371965879 1105 14-15 0.15848759017651304 1105 16-17 0.02563273861728277 1105 18-19 -0.1386903762845364 1105 20-21 -0.09579907201765536 1105 22-23 -0.29406276721834246 1105 24-25 -0.15954378172421002 1105 26-27 -0.3159061911944079 1105 28-29 -0.14102469982097432 1105 30-31 -0.06440529934086925 1105 32-33 -0.07180922835402725 1105 34-35 0.01605640776737971 1105 36-37 0.0028667239490260954 1105 38-39 0.04546015153395189 1105 40-41 -0.10665106398990076 1105 42-43 0.03879487493456679 1105 44-45 0.0886962121872088 1105 46-47 -0.08001147455935609 1105 48-49 -0.08321734913472767 1105 50-51 -0.04819408478404341 1105 52-53 -9.515001394007072E-4 1105 54-55 0.05720767995288867 1105 56-57 0.09931769175692295 1105 58-59 0.09478840932727906 1105 60-61 0.11067384544380587 1105 62-63 0.16331627677224247 1105 64-65 0.08548777674943864 1105 66-67 0.09419918440324437 1105 68-69 0.11687189665475373 1105 70-71 0.13741854919314633 1105 72-73 0.1270124966947357 1105 74-75 0.11040257585042923 1105 76-77 0.13279504071530468 1105 78-79 0.017117001881928218 1105 80-81 0.005413210783988376 1105 82-83 0.087641561353351 1105 84-85 0.08944567808696036 1105 86-87 0.16002235391623287 1105 88-89 0.1318754941767324 1105 90-91 0.23813473889033787 1105 92-93 0.2600614596150592 1105 94-95 0.2746220470889291 1105 96-97 0.20457795193120276 1105 98-99 0.23969701275365907 1105 100-101 0.14718939268028564 1105 102-103 0.2835526513038644 1105 104-105 0.23738049983321474 1105 106-107 0.2499977921616896 1105 108-109 0.2583688381089928 1105 110-111 0.3552309016445854 1105 112-113 0.2377813268139377 1105 114-115 0.13408632189077352 1105 116-117 0.25172419947004343 1105 118-119 0.1990038557357714 1105 120-121 0.1620173418488804 1105 122-123 0.2822283450819363 1105 124-125 0.4252148055571645 1106 1 -0.020290097417319686 1106 2 -0.06744675580502602 1106 3 0.13553274611355448 1106 4 0.18749057738430963 1106 5 0.1054617124727173 1106 6 -0.009359643508489057 1106 7 0.07069170617895537 1106 8 -0.02586753956148158 1106 9 -0.118177304489258 1106 10-11 -0.00146647172076797 1106 12-13 -0.0478266850666742 1106 14-15 -3.8875811394945003E-4 1106 16-17 0.11804597012282869 1106 18-19 -0.034076481113601176 1106 20-21 -0.10906005032524035 1106 22-23 -0.09194836126462036 1106 24-25 -3.9799725104973724 1106 26-27 -1.0691352778677938 1106 28-29 -0.6516915473164957 1106 30-31 -0.4402087548158775 1106 32-33 -5.421088672340531 1106 34-35 -1.1018193463711299 1106 36-37 -0.3984021896922769 1106 38-39 -0.045703423429003465 1106 40-41 -0.4953283009250171 1106 42-43 -0.2774627545165842 1106 44-45 -0.2785671896966946 1106 46-47 -0.2726763986934344 1106 48-49 -0.08879897222633559 1106 50-51 -0.09920866381682458 1106 52-53 -0.01741543887364827 1106 54-55 -0.18068588700192123 1106 56-57 0.003772833284855892 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-1.2543024345930398 2216 76-77 -0.988428157216287 2216 78-79 -0.8766308963875389 2216 80-81 -1.1473262961659394 2216 82-83 -1.1675627237749566 2216 84-85 -1.2156914132534773 2216 86-87 -1.0962229935349797 2216 88-89 -0.9704222746938846 2216 90-91 -1.0271121741576579 2216 92-93 -0.970881618439364 2216 94-95 -0.9455079224901226 2216 96-97 -1.1391819151675726 2216 98-99 -1.0011850181123698 2216 100-101 -0.9442093508184648 2216 102-103 -0.9955348886726405 2216 104-105 -0.9945390745609615 2216 106-107 -1.026889987848353 2216 108-109 -1.1603316057025879 2216 110-111 -1.1197464438045692 2216 112-113 -1.0684621113729094 2216 114-115 -1.0723724936344574 2216 116-117 -0.9761858171056588 2216 118-119 -1.0935871104464354 2216 120-121 -0.9804307169219726 2216 122-123 -0.8926993800234122 2216 124-125 -1.063460793181985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45455.0 3 8282.0 4 6307.0 5 5595.0 6 6057.0 7 5711.0 8 5410.0 9 5844.0 10 7035.0 11 8279.0 12 8916.0 13 9066.0 14 8603.0 15 8018.0 16 6979.0 17 6401.0 18 5851.0 19 5335.0 20 5196.0 21 5445.0 22 6528.0 23 9212.0 24 14221.0 25 20117.0 26 23270.0 27 20692.0 28 23910.0 29 28727.0 30 34445.0 31 42299.0 32 54401.0 33 69761.0 34 96245.0 35 138751.0 36 226316.0 37 313326.0 38 110130.0 39 245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.58478357479335 19.025793262821082 9.45366634076971 21.935756821615858 2 18.679899714017143 22.27011514685241 36.7202132001496 22.329771938980844 3 16.48962834395516 31.216647551410322 29.362171417275974 22.931552687358547 4 10.417285871325834 17.223441275222665 37.162660856927104 35.196611996524396 5 10.566965593544772 44.34954250819845 33.81215673893814 11.27133515931864 6 28.93180950376466 39.51694043312957 18.78795678801225 12.76329327509352 7 27.376488849670533 36.415000024886744 21.430689341574876 14.777821783867843 8 26.66074120424358 35.371597599476665 19.744659973264316 18.223001223015444 9 27.458689130507434 15.59271560615466 18.561964348820954 38.38663091451695 10-11 22.91721107104295 29.371562697429916 28.553525127217306 19.157701104309837 12-13 23.794713392010216 28.490392833627006 27.857333828232882 19.8575599461299 14-15 22.02029524886958 29.483111824389425 24.646546814103854 23.850046112637138 16-17 21.68959552882744 31.63518133029472 25.58814797959956 21.08707516127828 18-19 21.182842568054262 30.211449427983684 27.354354313783073 21.25135369017898 20-21 22.588414148539183 29.309923090925636 27.117630495130605 20.98403226540457 22-23 22.484054108391142 29.497506480659435 26.68683069510743 21.331608715841988 24-25 21.83552269498402 29.71797125316245 26.97558068924717 21.47092536260636 26-27 21.554012399447366 29.75117200304618 27.000297933733563 21.694517663772896 28-29 21.84681217484731 28.974546674976544 27.298764859742374 21.87987629043377 30-31 21.55137044987674 29.341002287460633 27.229456406347435 21.87817085631519 32-33 21.40359547126343 29.646372442186436 27.202035051667345 21.74799703488279 34-35 21.821298816914105 29.363049195283295 27.25833167717189 21.557320310630708 36-37 22.0355355323326 28.967509903176055 26.900588537700333 22.096366026791014 38-39 21.575261863402265 29.359489690783956 27.271693012401492 21.793555433412283 40-41 21.560987784417854 29.179772312840353 27.839823316868838 21.41941658587296 42-43 21.57171218139336 29.103969259605307 27.21479879234164 22.109519766659698 44-45 21.493153066481224 29.332258217049056 27.376143866486487 21.798444849983238 46-47 21.82918899239915 29.194965284382217 27.205496760954112 21.770348962264517 48-49 21.654344215202762 29.38783796082982 27.08059580083847 21.87722202312895 50-51 21.781173939228886 29.013999981512544 27.192950022007185 22.011876057251385 52-53 21.89484402447882 29.066557353619295 27.25062635167349 21.787972270228394 54-55 21.696141038977846 29.229686787835004 27.343153936785882 21.73101823640127 56-57 21.737113204139476 29.444090497325725 27.02366104886224 21.79513524967256 58-59 21.81520510249792 28.890299138917925 27.18530685381514 22.109188904769013 60-61 21.82606414106113 29.561409558942213 27.096124843479913 21.516401456516746 62-63 21.371593210721688 29.513721371664314 27.548796915735572 21.565888501878426 64-65 21.430417626143416 29.29700707248578 27.34996698937794 21.922608311992864 66-67 21.583693982372427 29.635876310504333 26.97929750674873 21.80113220037451 68-69 21.604149097895444 29.37638387403671 27.337565007416213 21.68190202065163 70-71 21.70215277323496 29.108656884522727 27.37934913379959 21.809841208442716 72-73 21.644457733106698 29.67874579993935 26.72004727034721 21.956749196606744 74-75 21.393859605773848 29.734235273580918 26.99520860538313 21.876696515262104 76-77 21.499117588920157 29.423965460112317 27.389655952115156 21.687260998852366 78-79 21.538087965096107 29.440668782743735 27.188852767680906 21.83239048447926 80-81 21.39792266984743 29.888383056409307 26.701567330134797 22.012126943608468 82-83 21.876847607633643 29.384138491779776 26.794368091969485 21.944645808617093 84-85 21.186121609942166 29.46926972102361 27.201806632448633 22.14280203658559 86-87 21.449080081770507 29.518620567060704 27.17543329481824 21.856866056350547 88-89 21.68550179344632 29.329788985192735 27.162330267877486 21.822378953483454 90-91 21.33170645687924 29.576976569566032 27.222343100280437 21.86897387327429 92-93 21.445659427371993 29.70252254820992 26.93188981178924 21.919928212628847 94-95 21.1701435144053 29.289744659050633 27.27701618594478 22.263095640599285 96-97 21.565859933118645 29.44016905923117 27.157023289018333 21.836947718631855 98-99 21.31294705058162 29.49258392436417 27.056561553303343 22.137907471750864 100-101 21.139856915270133 29.467789226088875 27.164624943871434 22.227728914769553 102-103 21.494889838302946 29.34868592032862 27.08508356119626 22.071340680172174 104-105 20.701543191661376 29.813256957090267 27.082149731471993 22.40305011977636 106-107 21.060333383343917 29.56201395224155 27.317617211414642 22.060035452999887 108-109 21.27980682161183 29.917514506333156 26.59612327778957 22.206555394265443 110-111 21.099435638066026 30.198091963193107 26.54939997881065 22.15307241993022 112-113 20.96835722282484 29.915139776367766 27.268719538187458 21.84778346261994 114-115 21.027069324904073 30.141097242867364 26.76410661344219 22.067726818786372 116-117 20.886582236357935 30.179569059854224 26.98452822058538 21.949320483202463 118-119 21.016021525102428 29.906538733380835 26.632016883325583 22.445422858191158 120-121 20.91971725730197 30.124462533606096 26.769506110424945 22.18631409866699 122-123 21.252070495137648 30.14672917763613 26.659124772952143 21.942075554274084 124-125 21.297017220893746 30.074880561557993 26.38912294249107 22.238979275057186 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1809.0 1 2078.5 2 2407.5 3 2199.0 4 1623.5 5 1243.5 6 1053.5 7 935.0 8 898.5 9 842.0 10 800.5 11 760.0 12 778.0 13 824.0 14 840.0 15 908.5 16 1024.5 17 1166.5 18 1428.0 19 1693.0 20 2055.5 21 2628.0 22 3461.0 23 4921.0 24 6303.0 25 7564.5 26 9339.5 27 10963.0 28 12789.0 29 15143.5 30 18984.5 31 23234.5 32 28107.5 33 33760.5 34 38313.5 35 41306.5 36 46607.0 37 52288.0 38 52718.5 39 52451.0 40 51152.0 41 49147.5 42 46285.0 43 45514.5 44 48102.5 45 49545.0 46 47799.0 47 44739.0 48 43485.0 49 41220.5 50 39753.0 51 38849.0 52 37203.5 53 36744.5 54 36716.0 55 35043.0 56 32061.5 57 29728.5 58 28244.5 59 25870.5 60 22461.5 61 19108.5 62 15810.5 63 12304.0 64 9364.5 65 7481.5 66 5770.0 67 4426.5 68 3517.5 69 2538.5 70 1908.0 71 1497.5 72 1260.0 73 1180.5 74 986.0 75 792.5 76 498.5 77 307.0 78 208.0 79 132.0 80 86.0 81 43.0 82 27.5 83 25.5 84 19.0 85 15.0 86 14.0 87 12.0 88 7.5 89 4.0 90 3.0 91 1.5 92 2.0 93 2.0 94 1.5 95 1.0 96 2.0 97 1.5 98 0.5 99 2.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.266269239985466E-4 2 2.133134619992733E-4 3 0.0 4 4.977314113316378E-4 5 0.0012087762846625487 6 8.532538479970932E-4 7 7.11044873330911E-4 8 0.001493194233994913 9 0.0022753435946589154 10-11 0.001955373401660005 12-13 0.0024886570566581886 14-15 0.002986388467989826 16-17 0.0027375227623240074 18-19 0.002844179493323644 20-21 0.0031997019299890997 22-23 0.08806290756203333 24-25 0.0024886570566581886 26-27 0.001990925645326551 28-29 0.0015998509649945499 30-31 0.0012798807719956399 32-33 0.002026477888993096 34-35 0.001173224040996003 36-37 9.954628226632755E-4 38-39 0.0012798807719956399 40-41 0.0015287464776614587 42-43 0.0016354032086610952 44-45 0.0015287464776614587 46-47 0.001813164426993823 48-49 3.555224366654555E-4 50-51 0.0015642987213280043 52-53 0.0015998509649945499 54-55 0.0012798807719956399 56-57 0.001350985259328731 58-59 7.821493606640021E-4 60-61 8.532538479970932E-4 62-63 0.001173224040996003 64-65 0.001102119553662912 66-67 3.1997019299890997E-4 68-69 2.133134619992733E-4 70-71 6.399403859978199E-4 72-73 1.7776121833272776E-4 74-75 3.9107468033200107E-4 76-77 4.977314113316378E-4 78-79 3.555224366654555E-4 80-81 6.043881423312743E-4 82-83 1.0665673099963665E-4 84-85 5.332836549981833E-4 86-87 4.266269239985466E-4 88-89 7.465971169974565E-4 90-91 3.555224366654555E-4 92-93 3.9107468033200107E-4 94-95 4.6217916766509216E-4 96-97 7.11044873330911E-4 98-99 7.465971169974565E-4 100-101 8.177016043305476E-4 102-103 0.001173224040996003 104-105 7.11044873330911E-4 106-107 8.532538479970932E-4 108-109 8.888060916636388E-4 110-111 9.954628226632755E-4 112-113 6.754926296643655E-4 114-115 7.465971169974565E-4 116-117 9.954628226632755E-4 118-119 0.0016354032086610952 120-121 9.954628226632755E-4 122-123 0.0010310150663298211 124-125 5.688358986647289E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1406381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.871914964464708 #Duplication Level Percentage of deduplicated Percentage of total 1 60.310477178675725 18.618999229263665 2 11.195995631869282 6.9128365017917375 3 7.647108791241854 7.08242676981687 4 5.579452116863762 6.889934852004827 5 3.8087074576799917 5.879104637900965 6 2.6845740827085445 4.9726765678310425 7 1.8027512957335563 3.8958069292775446 8 1.3075434162942765 3.2293095328146073 9 0.9434883121988156 2.6214561849751354 >10 4.271344964732235 22.896175797751877 >50 0.27812209133196225 5.953950596587855 >100 0.16101711802249963 8.951246301762513 >500 0.008269061836824099 1.603595009478561 >1k 0.0011484808106700139 0.49248108874275254 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2522 0.17932551705405578 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.11044873330911E-5 0.0 0.0 0.0 0.0 8 7.11044873330911E-5 0.0 0.0 0.0 0.0 9 7.11044873330911E-5 0.0 0.0 0.0 0.0 10-11 7.11044873330911E-5 0.0 0.0 0.0 0.0 12-13 7.11044873330911E-5 0.0 0.0 0.0 0.0 14-15 7.11044873330911E-5 0.0 0.0 0.0 0.0 16-17 7.11044873330911E-5 0.0 0.0 3.555224366654555E-5 0.0 18-19 4.6217916766509216E-4 0.0 0.0 7.11044873330911E-5 0.0 20-21 6.399403859978199E-4 0.0 0.0 7.11044873330911E-5 0.0 22-23 6.399403859978199E-4 0.0 0.0 1.0665673099963667E-4 0.0 24-25 0.0014931942339949133 0.0 0.0 4.6217916766509216E-4 0.0 26-27 0.0014931942339949133 0.0 0.0 7.11044873330911E-4 0.0 28-29 0.0014931942339949133 0.0 0.0 0.0012087762846625487 0.0 30-31 0.0014931942339949133 0.0 0.0 0.004515134945651285 0.0 32-33 0.0014931942339949133 0.0 0.0 0.013047673425622217 0.0 34-35 0.0015998509649945499 0.0 0.0 0.022824540433922243 0.0 36-37 0.0017065076959941864 0.0 0.0 0.04056511002352847 0.0 38-39 0.0018487166706603686 0.0 0.0 0.07050009919075984 0.0 40-41 0.0018487166706603686 0.0 0.0 0.10196383483565263 0.0 42-43 0.0018487166706603686 0.0 0.0 0.14043136248285493 0.0 44-45 0.0018487166706603686 0.0 0.0 0.1879291600213598 0.0 46-47 0.002275343594658915 0.0 0.0 0.24609263065982828 0.0 48-49 0.002630866031324371 0.0 0.0 0.3062470269436234 0.0 50-51 0.002630866031324371 0.0 0.0 0.3678235129740803 0.0 52-53 0.002630866031324371 0.0 0.0 0.43953238844950265 0.0 54-55 0.002630866031324371 0.0 0.0 0.5254621613915433 0.0 56-57 0.002630866031324371 0.0 0.0 0.6298791010401875 0.0 58-59 0.002630866031324371 0.0 0.0 0.7531031775884345 0.0 60-61 0.002630866031324371 0.0 0.0 0.8740163582983559 0.0 62-63 0.002630866031324371 0.0 0.0 1.0023955101782518 0.0 64-65 0.002630866031324371 0.0 0.0 1.1448177983064334 0.0 66-67 0.002630866031324371 0.0 0.0 1.3203392252881687 0.0 68-69 0.002630866031324371 0.0 0.0 1.52739549240213 0.0 70-71 0.002630866031324371 0.0 0.0 1.7431976114580614 0.0 72-73 0.0026664182749909166 0.0 0.0 1.9544845955683416 0.0 74-75 0.0027019705186574617 0.0 0.0 2.1821611640088996 0.0 76-77 0.0027019705186574617 0.0 0.0 2.4545980072256377 0.0 78-79 0.0027019705186574617 0.0 0.0 2.7822119326128556 0.0 80-81 0.0027019705186574617 0.0 0.0 3.1081193503040785 0.0 82-83 0.0027019705186574617 0.0 0.0 3.44710999366459 0.0 84-85 0.0027019705186574617 0.0 0.0 3.8118049091960144 0.0 86-87 0.0027019705186574617 0.0 0.0 4.206043739214338 0.0 88-89 0.0027019705186574617 0.0 0.0 4.638465678930531 0.0 90-91 0.002844179493323644 0.0 0.0 5.106262101094938 0.0 92-93 0.002844179493323644 0.0 0.0 5.594607720098608 0.0 94-95 0.002844179493323644 0.0 0.0 6.085939727570267 0.0 96-97 0.002844179493323644 0.0 0.0 6.641976818515039 0.0 98-99 0.0030574929553229173 0.0 0.0 7.245831677191315 0.0 100-101 0.0033063586609887365 0.0 0.0 7.900846214503751 0.0 102-103 0.003413015391988373 0.0 0.0 8.537977973251914 0.0 104-105 0.0035196721229880097 0.0 0.0 9.190397196776692 0.0 106-107 0.0036974333413207373 0.0 0.0 9.87324914088003 0.0 108-109 0.0036974333413207373 0.0 0.0 10.586711566780266 0.0 110-111 0.0036974333413207373 0.0 0.0 11.360435045695299 0.0 112-113 0.0036974333413207373 0.0 0.0 12.13714491307832 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2325 0.0 60.900524 1 GTACATG 12725 0.0 56.43084 1 TACATGG 13100 0.0 53.271355 2 ACATGGG 12930 0.0 51.34908 3 CATGGGA 8990 0.0 49.169537 4 ATGGGAT 3520 0.0 45.46494 5 GTCGGGA 405 0.0 44.069008 2 ATGGGAG 3115 0.0 43.16357 5 CATGGGG 3325 0.0 40.974236 4 GTATCAA 3455 0.0 40.12129 2 CATGGGT 1215 0.0 35.255207 4 CGGGAGT 540 0.0 35.255203 4 TCAACGC 3960 0.0 34.854576 5 CAACGCA 3975 0.0 34.57338 6 GGGATAC 330 0.0 34.254852 7 ATCAACG 4095 0.0 33.99609 4 AACGCAG 4105 0.0 33.479675 7 ATGGGAC 1685 0.0 33.18906 5 GAGTACA 7220 0.0 31.312187 1 ATGGGTA 775 0.0 30.706146 5 >>END_MODULE