FastQCFastQC Report
Thu 26 May 2016
SRR936207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406381
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98550.7007347226676128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77960.5543305832487783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74880.5324304011501862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22510.16005620098678808No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.15223470738014805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.14007584004618948No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT14070.10004401367765918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45700.091.378911
GTATCAA74500.056.103912
GTACATG104800.046.043451
TACATGG109750.043.5635532
ACATGGG107000.043.4589773
CATGGGA75050.041.6505624
ATCAACG108850.040.204184
TCAACGC109100.040.1666225
CAACGCA109800.039.9891246
TATCACG758.156838E-539.693652
AGCGTAC600.001304244239.693653
AACGCAG112300.039.09897
TATAACG1252.9582225E-838.1059042
TATCAAC116600.037.3276863
ATGGGAT33400.035.6529775
CATGGGG29500.034.1096274
ATCACGC1051.4770279E-534.0231253
ATGGGAG28950.033.3179865
ACGCAGA131650.033.1713458
CGCAGAG132950.032.667999