##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936207_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1406381 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.554471370133697 31.0 31.0 34.0 30.0 34.0 2 31.407070345802452 31.0 31.0 34.0 30.0 34.0 3 31.711755207159367 31.0 31.0 34.0 30.0 34.0 4 35.59200885108658 37.0 35.0 37.0 33.0 37.0 5 35.206958142921444 37.0 35.0 37.0 33.0 37.0 6 35.40940755030109 37.0 35.0 37.0 33.0 37.0 7 35.25527079788478 37.0 35.0 37.0 32.0 37.0 8 35.33591537428335 37.0 35.0 37.0 32.0 37.0 9 37.01001720017548 39.0 37.0 39.0 33.0 39.0 10-11 36.88970271924891 39.0 37.0 39.0 32.5 39.0 12-13 36.84259741848048 39.0 37.0 39.0 32.0 39.0 14-15 37.9760964489708 40.0 37.0 41.0 33.0 41.0 16-17 37.894309578983226 40.0 37.0 41.0 32.5 41.0 18-19 38.04063692555573 40.0 37.0 41.0 33.0 41.0 20-21 38.10315519052092 40.0 37.0 41.0 33.0 41.0 22-23 37.98529594754196 40.0 37.0 41.0 33.0 41.0 24-25 37.94341398241302 40.0 37.0 41.0 33.0 41.0 26-27 37.75036316616905 40.0 37.0 41.0 32.0 41.0 28-29 37.58867049540629 40.0 36.5 41.0 32.0 41.0 30-31 37.36492209436845 39.5 36.0 41.0 32.0 41.0 32-33 37.08884327931052 39.0 35.5 41.0 31.0 41.0 34-35 36.84919520386012 39.0 35.0 41.0 31.0 41.0 36-37 36.60803260282953 39.0 35.0 41.0 30.0 41.0 38-39 36.561072710737704 39.0 35.0 41.0 30.0 41.0 40-41 36.541103370992644 39.0 35.0 41.0 30.0 41.0 42-43 36.394186568220135 39.0 35.0 41.0 30.0 41.0 44-45 36.1628886482397 39.0 35.0 41.0 30.0 41.0 46-47 35.78630648451593 39.0 35.0 41.0 28.0 41.0 48-49 35.448838543751656 39.0 35.0 41.0 26.0 41.0 50-51 35.25238608883368 39.0 35.0 41.0 26.0 41.0 52-53 35.11621708484401 39.0 35.0 41.0 25.5 41.0 54-55 34.93772775656099 39.0 34.5 41.0 25.0 41.0 56-57 34.73640642187288 38.5 34.0 40.5 24.5 41.0 58-59 34.51781096303207 38.0 34.0 40.0 24.0 41.0 60-61 34.30290013872485 38.0 34.0 40.0 23.5 41.0 62-63 34.07114963868255 37.5 34.0 40.0 23.0 41.0 64-65 33.81815951722898 37.0 33.5 40.0 23.0 41.0 66-67 33.53973212095442 36.5 33.0 40.0 22.5 41.0 68-69 33.22976526275596 36.0 33.0 39.0 22.0 41.0 70-71 32.90962904077914 35.5 33.0 39.0 22.0 41.0 72-73 32.637911774974214 35.0 33.0 39.0 21.5 40.0 74-75 32.43711341379043 35.0 33.0 37.5 21.5 40.0 76-77 32.13080310385308 35.0 32.5 37.0 20.5 39.0 78-79 31.796750667137854 35.0 32.0 37.0 20.0 39.0 80-81 31.518682348524333 35.0 32.0 36.0 20.0 39.0 82-83 31.24973886877027 35.0 32.0 36.0 19.0 37.0 84-85 30.974626719217625 35.0 32.0 35.5 18.0 37.0 86-87 30.78193675824688 35.0 32.0 35.0 18.0 36.5 88-89 30.611731813783035 35.0 32.0 35.0 16.5 36.0 90-91 30.462258804690904 35.0 32.0 35.0 14.5 36.0 92-93 30.32442133390596 35.0 31.0 35.0 11.0 36.0 94-95 30.194578140631876 35.0 31.0 35.0 10.0 35.5 96-97 30.122337048068765 34.0 31.0 35.0 7.5 35.0 98-99 30.036226669728897 34.0 31.0 35.0 7.0 35.0 100-101 29.943785147836895 34.0 31.0 35.0 6.0 35.0 102-103 28.934875044529186 33.5 29.5 34.5 2.0 35.0 104-105 29.670983538600137 34.0 31.0 35.0 2.0 35.0 106-107 29.80900339239509 34.0 31.0 35.0 2.0 35.0 108-109 29.773211526606232 34.0 31.0 35.0 2.0 35.0 110-111 29.73103376680999 34.0 31.0 35.0 2.0 35.0 112-113 29.640114591991786 34.0 31.0 35.0 2.0 35.0 114-115 29.611142357583045 34.0 31.0 35.0 2.0 35.0 116-117 29.510594924135066 34.0 31.0 35.0 2.0 35.0 118-119 29.372320160753027 34.0 31.0 35.0 2.0 35.0 120-121 29.173457619236892 34.0 31.0 35.0 2.0 35.0 122-123 28.949803787167205 34.0 31.0 35.0 2.0 35.0 124-125 26.896994839947354 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21142514094829323 1101 2 0.6910050049119825 1101 3 0.4041073092364478 1101 4 0.2626499119258341 1101 5 0.4758213164066163 1101 6 0.32351425060628003 1101 7 0.4139100762496426 1101 8 0.3999737583930383 1101 9 0.27984484403439325 1101 10-11 0.3801855778058538 1101 12-13 0.41544936783481745 1101 14-15 0.5070788844379379 1101 16-17 0.5523603106326931 1101 18-19 0.5321706086669238 1101 20-21 0.4014357020588335 1101 22-23 0.34374664759361906 1101 24-25 0.37985325746470977 1101 26-27 0.37645785058919756 1101 28-29 0.4207251067354889 1101 30-31 0.3528864627988355 1101 32-33 0.2799013485720181 1101 34-35 0.2998570570879693 1101 36-37 0.3277934629120196 1101 38-39 0.3694507217211651 1101 40-41 0.2660203215702168 1101 42-43 0.33938906142755343 1101 44-45 0.33504891742212806 1101 46-47 0.2389007119335247 1101 48-49 0.1508340728644697 1101 50-51 0.13708160699370353 1101 52-53 0.15621553880587413 1101 54-55 0.13641324942218347 1101 56-57 -0.13703522383269728 1101 58-59 0.0772148642894308 1101 60-61 0.24546131319471698 1101 62-63 0.19485352934774625 1101 64-65 0.08274609377546938 1101 66-67 0.14771279478279808 1101 68-69 0.07387582675638527 1101 70-71 0.034047366113057365 1101 72-73 0.08480303871586159 1101 74-75 0.09124179631486129 1101 76-77 0.19151352019646595 1101 78-79 0.31240630973730177 1101 80-81 0.18726996246524052 1101 82-83 0.24446179109349586 1101 84-85 0.139451607836925 1101 86-87 0.07672945937491704 1101 88-89 0.0806417781978297 1101 90-91 0.25117035470632487 1101 92-93 0.3290345685895275 1101 94-95 0.098890748026637 1101 96-97 0.09949985536793804 1101 98-99 0.3260556956862004 1101 100-101 0.3151451520575641 1101 102-103 0.34154763390291265 1101 104-105 0.4040426893618765 1101 106-107 0.3012175257277221 1101 108-109 0.24688611850426412 1101 110-111 0.2308927572178341 1101 112-113 0.3198073013880425 1101 114-115 0.3164783422422275 1101 116-117 0.3253726718451979 1101 118-119 0.35147837020871364 1101 120-121 0.34143731214269835 1101 122-123 0.5005461930876685 1101 124-125 0.5109859214564594 1102 1 0.24512871862669172 1102 2 0.6350989189699199 1102 3 0.4070627826955153 1102 4 0.1882648254004593 1102 5 0.4615543516000784 1102 6 0.3109705018215294 1102 7 0.356094436869256 1102 8 0.3973245264272549 1102 9 0.4389445912636347 1102 10-11 0.36016119190916385 1102 12-13 0.2533325747827746 1102 14-15 0.4675486167601903 1102 16-17 0.5340364562027204 1102 18-19 0.4762657379688946 1102 20-21 0.40795589131283805 1102 22-23 0.42724646935782573 1102 24-25 0.36647747160692035 1102 26-27 0.4861672452356487 1102 28-29 0.45781799998845685 1102 30-31 0.36375884597337915 1102 32-33 0.3777329481572167 1102 34-35 0.5203695659997578 1102 36-37 0.6494086032322031 1102 38-39 0.5667690832481611 1102 40-41 0.3230257982699456 1102 42-43 0.28436767313278466 1102 44-45 0.53067606435323 1102 46-47 0.5833510975709686 1102 48-49 0.5405462220640231 1102 50-51 0.33939259604031236 1102 52-53 0.5352250339124964 1102 54-55 0.5178183955755387 1102 56-57 0.5100506210049431 1102 58-59 0.5397282319736547 1102 60-61 0.3771581179131047 1102 62-63 0.38458188180091213 1102 64-65 0.35249542944207235 1102 66-67 0.3299410662197033 1102 68-69 0.2698699773818305 1102 70-71 0.3657607791663935 1102 72-73 0.5129326971512711 1102 74-75 0.43452919342420415 1102 76-77 0.48460283253038483 1102 78-79 0.325293567498651 1102 80-81 0.2802389656410753 1102 82-83 0.2856468160465049 1102 84-85 0.3052011703490791 1102 86-87 0.3081682272795163 1102 88-89 0.30337615826134723 1102 90-91 0.2730852552183478 1102 92-93 0.3892859620693372 1102 94-95 0.25330370414822667 1102 96-97 0.31564396710557574 1102 98-99 0.33082917420068725 1102 100-101 0.30573713792832535 1102 102-103 0.256323785954244 1102 104-105 0.31739741098997953 1102 106-107 0.26284579068332903 1102 108-109 0.3127507241170697 1102 110-111 0.30722061385144883 1102 112-113 0.320837828317849 1102 114-115 0.34116804993553274 1102 116-117 0.3484351677943849 1102 118-119 0.23760798003436534 1102 120-121 0.2670781508231066 1102 122-123 0.2972387039438473 1102 124-125 0.34641149991261244 1103 1 0.19937391516229042 1103 2 0.6780376397118708 1103 3 0.35643941129790946 1103 4 0.2326879472548029 1103 5 0.4122431915970921 1103 6 0.16797657490622697 1103 7 0.32457154963100976 1103 8 0.2390215341796491 1103 9 0.23422987859534317 1103 10-11 0.32550613516971083 1103 12-13 0.32671946743374036 1103 14-15 0.3365495594431209 1103 16-17 0.4638687405781994 1103 18-19 0.36155686230893025 1103 20-21 0.23679850058130114 1103 22-23 0.29118566306375726 1103 24-25 0.21383422760941784 1103 26-27 0.29634045308344525 1103 28-29 0.14309951090962159 1103 30-31 0.023890010324706168 1103 32-33 0.23888506164910694 1103 34-35 0.24750484018906604 1103 36-37 0.18403881516550058 1103 38-39 0.16455580650824686 1103 40-41 0.05488584473885538 1103 42-43 0.10377817991004434 1103 44-45 0.2587944830147819 1103 46-47 0.24384286653739196 1103 48-49 0.2208431550183363 1103 50-51 0.10377996976785653 1103 52-53 0.0776463742421285 1103 54-55 0.19355858826576622 1103 56-57 0.1743250877644229 1103 58-59 0.12199506397490723 1103 60-61 0.1176217686434029 1103 62-63 0.0993565100009306 1103 64-65 0.20303617411290986 1103 66-67 0.21584235871750934 1103 68-69 0.004111170354377691 1103 70-71 0.1523725925573629 1103 72-73 0.22423909181902957 1103 74-75 0.18462096709592402 1103 76-77 0.17544542232864302 1103 78-79 0.18938152581941736 1103 80-81 0.31674795195676353 1103 82-83 0.2611187051117092 1103 84-85 0.26181504028154734 1103 86-87 0.16557029615215768 1103 88-89 0.2114397277034925 1103 90-91 0.2092537764230329 1103 92-93 0.18328611377476633 1103 94-95 0.13015560557701278 1103 96-97 0.24365710463344215 1103 98-99 0.24555855443128038 1103 100-101 0.18758203413585406 1103 102-103 0.15741519293691297 1103 104-105 0.10550832956229428 1103 106-107 0.10083595335697737 1103 108-109 0.16502452077662966 1103 110-111 0.25290905633156413 1103 112-113 0.24325388735154263 1103 114-115 0.34520751119467263 1103 116-117 0.34179430321946924 1103 118-119 0.37225455911751126 1103 120-121 0.36620324857981146 1103 122-123 0.13867965808175242 1103 124-125 0.19259852187826 1104 1 0.17532337221447492 1104 2 0.5002382309760023 1104 3 0.3434561925871158 1104 4 0.13059372861629726 1104 5 0.4035052671433874 1104 6 0.27510756280836546 1104 7 0.35033042677026316 1104 8 0.3112890575776248 1104 9 0.3080151360811385 1104 10-11 0.3407407166510481 1104 12-13 0.3229394676033408 1104 14-15 0.28630735193856793 1104 16-17 0.3322918760387452 1104 18-19 0.3537178273269461 1104 20-21 0.18299485745414756 1104 22-23 0.2573377902134055 1104 24-25 0.26778203225561725 1104 26-27 0.15364134563303367 1104 28-29 0.08074349576013873 1104 30-31 0.15710787310797514 1104 32-33 0.10197886476323248 1104 34-35 0.004409147226454024 1104 36-37 0.07419106911267193 1104 38-39 0.02888497692534031 1104 40-41 -0.10249774887264351 1104 42-43 -0.03775350190569782 1104 44-45 -0.06120814985105483 1104 46-47 -0.09225336067085976 1104 48-49 -0.07632825060493076 1104 50-51 0.03705418476396005 1104 52-53 -0.013341350222809467 1104 54-55 -0.12159585637116521 1104 56-57 -0.2139527635747669 1104 58-59 -0.19171796737840907 1104 60-61 -0.23903827143914924 1104 62-63 -0.25731948072589006 1104 64-65 -0.3199043857102879 1104 66-67 -0.2596440248837837 1104 68-69 -0.19039522585396895 1104 70-71 -0.10927975689177316 1104 72-73 -0.16897437343218513 1104 74-75 -0.19349450668465096 1104 76-77 -0.21452622590594217 1104 78-79 -0.19103397029206093 1104 80-81 -0.19212989397131963 1104 82-83 -0.33170909779001434 1104 84-85 -0.41350515375904706 1104 86-87 -0.22630573786349828 1104 88-89 -0.34627656808040896 1104 90-91 -0.308661522829226 1104 92-93 -0.3798970129472501 1104 94-95 -0.2886650037860541 1104 96-97 -0.20098547777182318 1104 98-99 -0.22177028606995108 1104 100-101 -0.20375904757626984 1104 102-103 -0.23438452027454204 1104 104-105 -0.12021731370296251 1104 106-107 -0.07129168894800841 1104 108-109 -0.15779602559148742 1104 110-111 -0.2454339947803561 1104 112-113 -0.22561721892456887 1104 114-115 -0.13386423399560243 1104 116-117 -0.17473096805253263 1104 118-119 -0.1722983160567857 1104 120-121 -0.057426480029061366 1104 122-123 -0.19766128027647412 1104 124-125 -0.07509165119228811 1105 1 0.16432937674201042 1105 2 0.5197822508663243 1105 3 0.18416812904427005 1105 4 0.0951765130942519 1105 5 0.3246804951592708 1105 6 0.22147132885630327 1105 7 0.2792889704567685 1105 8 0.27087561897532453 1105 9 0.19480807624130136 1105 10-11 0.08789421853713009 1105 12-13 0.19257146329912445 1105 14-15 0.1709486796659263 1105 16-17 0.3067073674098424 1105 18-19 0.1987296110781287 1105 20-21 0.11848294237504575 1105 22-23 0.12439677175240149 1105 24-25 0.08766776304886292 1105 26-27 0.1087361341990416 1105 28-29 0.10601379089234797 1105 30-31 0.11442063345417353 1105 32-33 0.19701816578870535 1105 34-35 0.196090307648781 1105 36-37 0.13571383334903686 1105 38-39 0.20112950436532628 1105 40-41 0.03296949444482067 1105 42-43 0.054809641794875574 1105 44-45 0.11450603765820944 1105 46-47 0.24244073664532095 1105 48-49 0.08078902781942077 1105 50-51 0.18241095764929582 1105 52-53 0.12894799163154147 1105 54-55 0.15767229142024064 1105 56-57 0.12854670296760418 1105 58-59 0.15064818442714056 1105 60-61 0.19269743080366908 1105 62-63 0.2318645416916496 1105 64-65 0.2340935464211924 1105 66-67 0.2644568357611945 1105 68-69 0.3778486077930765 1105 70-71 0.2756819434333835 1105 72-73 0.307649453706091 1105 74-75 0.4293519437459139 1105 76-77 0.307293681183026 1105 78-79 0.28542246837263363 1105 80-81 0.38031047301573295 1105 82-83 0.254798159419213 1105 84-85 0.16338546179741087 1105 86-87 0.18877496026862417 1105 88-89 0.2507396057190938 1105 90-91 0.20777026353659167 1105 92-93 0.12924209968920408 1105 94-95 0.15128485318372142 1105 96-97 0.17137609245722984 1105 98-99 -0.021443734502977208 1105 100-101 -0.01424599008324634 1105 102-103 -0.0566157758450494 1105 104-105 -0.07693492606980001 1105 106-107 -0.08972548119397672 1105 108-109 0.03044527686368781 1105 110-111 0.07336435664682739 1105 112-113 0.10693917360646665 1105 114-115 -0.04731137957013232 1105 116-117 0.037767556743411035 1105 118-119 0.018291988087575817 1105 120-121 0.01965884259458761 1105 122-123 0.12630704048633135 1105 124-125 0.12816901666884917 1106 1 0.15034977987293274 1106 2 0.5560748366484773 1106 3 0.28897312743559667 1106 4 0.10471817066076028 1106 5 0.2916252155438457 1106 6 0.18428692804562985 1106 7 0.23402304636261562 1106 8 0.11262688771282825 1106 9 0.034718765574979216 1106 10-11 0.21752649014675995 1106 12-13 0.22736604641816172 1106 14-15 0.2775886799477334 1106 16-17 0.19586691247263133 1106 18-19 0.21008542824841214 1106 20-21 0.1578337667902332 1106 22-23 0.07696677248041084 1106 24-25 0.07430961858774054 1106 26-27 0.1802163710143816 1106 28-29 0.08030849965221165 1106 30-31 0.08598265422835993 1106 32-33 0.04860934228000957 1106 34-35 0.16583493973251962 1106 36-37 0.3367285885138145 1106 38-39 0.25151969545680686 1106 40-41 0.2516297405129748 1106 42-43 0.17452538823054908 1106 44-45 0.20471262875250318 1106 46-47 0.06570489707104343 1106 48-49 0.19741160277533254 1106 50-51 0.1958451324238979 1106 52-53 0.21530081455781414 1106 54-55 0.15049638017198674 1106 56-57 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-0.478866429525695 2216 124-125 -0.6435256669126694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54.0 3 64.0 4 173.0 5 450.0 6 1007.0 7 1834.0 8 2955.0 9 4837.0 10 8243.0 11 10195.0 12 11051.0 13 13538.0 14 18948.0 15 14271.0 16 7061.0 17 6082.0 18 5866.0 19 5559.0 20 5588.0 21 5891.0 22 6372.0 23 7159.0 24 9028.0 25 11373.0 26 14244.0 27 17778.0 28 22365.0 29 27886.0 30 35007.0 31 43731.0 32 54852.0 33 70115.0 34 93990.0 35 133684.0 36 223348.0 37 358442.0 38 152762.0 39 578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.38011253191108 18.659692002948482 11.095094303107576 21.86510116203286 2 18.956710073678625 22.16978650121091 36.55141078714257 22.322092637967884 3 17.353773517825907 28.811416544603873 32.21085339666377 21.62395654090644 4 11.156862898460659 16.84422642228528 37.5818501529813 34.41706052627275 5 10.089853294644522 42.131361702914894 35.47091864107209 12.307866361368491 6 29.188392050233897 38.674086182904915 18.015388433148626 14.122133333712558 7 25.723825904929033 35.00701445767541 23.395011735795634 15.874147901599923 8 27.378071802733402 32.735439400845145 20.901092947074797 18.985395849346656 9 27.04729372765986 11.411914694524457 20.569106095716595 40.97168548209909 10-11 23.70236088229292 26.162042860362877 30.084415247361846 20.051181009982358 12-13 25.142226750788016 25.938383695456636 29.980993770535864 18.938395783219484 14-15 22.462903011346143 26.832273757964593 28.12865077102151 22.576172459667756 16-17 20.6393291147026 28.802376028393457 28.990589673278322 21.567705183625627 18-19 19.847004474605388 26.30215425265273 32.97175516449668 20.879086108245204 20-21 21.01507344026974 25.5882651998285 33.551434497479704 19.84522686242206 22-23 20.791058752926837 25.84655225006595 33.198187404408905 20.164201592598307 24-25 20.339189735925046 26.16342939786587 33.25915950229703 20.238221363912054 26-27 20.139990514661392 26.3501142293589 33.15136509950006 20.358530156479645 28-29 20.311395102534487 25.582870354075588 33.586358741464345 20.519375801925584 30-31 20.252335604647673 25.75187662518194 33.48527177201626 20.510515998154126 32-33 20.00361210795652 26.30563837253205 33.17923805853464 20.51151146097679 34-35 20.564158025681557 26.167306252439776 32.96419386239414 20.304341859484534 36-37 20.803644247184796 25.65293473105794 32.40259929563895 21.140821726118315 38-39 20.493450921194185 26.254158723702893 32.61132651820524 20.641063836897683 40-41 20.560040273581627 26.16332274113487 32.81155675453522 20.46508023074828 42-43 20.78985906319207 26.24611624717929 31.824285672129665 21.139739017498975 44-45 20.50184107714035 26.525342010834553 31.799683127086237 21.173133784938866 46-47 21.11566109042346 26.516186678812588 31.07471127661231 21.29344095415164 48-49 21.111674071071622 27.024780312282783 30.341775954972682 21.521769661672916 50-51 21.209582797044664 26.925199286054596 30.16469099106146 21.700526925839277 52-53 21.248855586073258 26.90918464396505 30.133454218998608 21.708505550963086 54-55 21.192490703332577 27.322086904951952 30.051159670151854 21.43426272156361 56-57 21.311361169370997 27.155478457748362 29.860459526791868 21.672700846088784 58-59 21.203772539758894 26.646806328998263 30.23940844210121 21.910012689141627 60-61 21.404268239353044 27.229242409913386 30.019426298458196 21.347063052275374 62-63 21.0826457975285 27.025223432841443 30.651238559932764 21.240892209697297 64-65 21.035010294426645 26.758007556475356 30.62356387326425 21.58341827583375 66-67 21.26594258624631 27.195822049574943 30.063736569261035 21.47449879491771 68-69 21.31488324766257 26.918277274624508 30.27188053070602 21.494958947006904 70-71 21.474006061845042 26.695547832579308 30.205942741340543 21.624503364235114 72-73 21.406477651611308 27.251424439130425 29.496289312066832 21.84580859719144 74-75 21.03657864554558 27.448454066399453 29.706186223084018 21.80878106497095 76-77 21.06236358975763 27.142540726201116 30.105836038577234 21.689259645464027 78-79 20.998202829610115 27.214848075996667 29.789939045526538 21.99701004886668 80-81 21.037725753507157 27.617130157813698 29.186627876591814 22.158516212087328 82-83 21.475015438589256 27.019575455682805 29.27042755559759 22.234981550130353 84-85 20.781043185123746 27.128885564346078 29.57179643701131 22.518274813518865 86-87 20.98403983003123 27.285057481071917 29.45368095018686 22.27722173870999 88-89 21.113475366415134 27.080144699535147 29.378556382156095 22.427823551893628 90-91 20.9615878255144 27.29674170957321 29.36638861848856 22.37528184642383 92-93 21.073540129766243 27.34228068616123 29.115563061061238 22.468616123011287 94-95 20.845760443127418 26.844512547396036 29.433060704261155 22.876666305215387 96-97 21.223881626452375 27.058334574716387 29.20109801071773 22.51668578811351 98-99 20.99261813280677 27.165399261848833 28.973517644770887 22.86846496057351 100-101 20.99870766353745 26.8066490444354 29.206093002006945 22.988550290020214 102-103 21.322500215982107 26.572945245780527 29.022840063383192 23.081714474854174 104-105 20.70855565541878 26.959835548021573 29.06113478122369 23.270474015335957 106-107 21.002173320245298 26.65397918831662 29.211087950675974 23.13275954076211 108-109 21.32300553341022 26.926454422248547 28.49069872280758 23.259841321533656 110-111 21.004433415222934 27.150988541381505 28.36198284229201 23.482595201103553 112-113 20.97256559038009 26.708272635245766 29.055044113301832 23.26411766107231 114-115 20.91328468066933 26.906430920649075 28.617239514269894 23.563044884411696 116-117 20.686928167326286 27.03406193661258 28.665294090454907 23.613715805606226 118-119 20.71816387846895 26.74251986701986 28.60278404949421 23.936532205016977 120-121 20.575113631838533 26.89842678923925 28.565998907769064 23.960460671153157 122-123 20.6486818583259 27.06232960581612 28.434727205755824 23.854261330102155 124-125 20.701484922340676 26.837931383895004 28.112621573239043 24.34796212052528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 135.0 1 147.5 2 551.0 3 1066.0 4 903.0 5 855.0 6 1428.5 7 2971.0 8 4365.5 9 4953.5 10 4332.5 11 2993.0 12 2663.5 13 2695.0 14 2827.0 15 2973.5 16 3200.0 17 3443.0 18 3627.0 19 3898.5 20 4188.5 21 4561.0 22 5197.0 23 6324.5 24 7394.5 25 8424.0 26 10185.5 27 11764.5 28 13005.5 29 15221.0 30 19047.5 31 23136.0 32 27952.5 33 33162.5 34 37322.0 35 39918.5 36 44882.5 37 50816.0 38 51635.0 39 51371.0 40 49952.0 41 47487.5 42 44646.0 43 44172.0 44 46838.5 45 48154.5 46 45996.0 47 43091.5 48 42427.5 49 40863.5 50 39887.0 51 38588.0 52 36266.5 53 35284.0 54 35170.0 55 33896.5 56 31096.5 57 28792.0 58 27065.0 59 24829.5 60 21864.0 61 18295.0 62 15179.0 63 12171.5 64 9193.0 65 7138.0 66 5399.0 67 4198.0 68 3231.5 69 2151.0 70 1613.5 71 1338.5 72 1099.5 73 1053.0 74 889.0 75 683.0 76 439.5 77 216.0 78 108.5 79 68.0 80 36.5 81 15.5 82 8.5 83 2.5 84 2.5 85 2.0 86 2.0 87 2.5 88 1.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06491839693511217 2 2.133134619992733E-4 3 7.11044873330911E-5 4 0.0 5 0.0696112930990962 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.555224366654555E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.555224366654555E-5 30-31 0.0 32-33 0.0 34-35 1.422089746661822E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002950836224323281 44-45 0.009883523739299663 46-47 0.010487911881630937 48-49 0.008781404185636752 50-51 0.007892598093973113 52-53 0.006648269565644019 54-55 0.006932687514976382 56-57 0.006150538154312381 58-59 0.004301821483652012 60-61 0.002844179493323644 62-63 0.002133134619992733 64-65 0.002666418274990916 66-67 0.003235254173655645 68-69 0.001990925645326551 70-71 0.0030930451989894634 72-73 0.002701970518657462 74-75 9.954628226632755E-4 76-77 5.332836549981833E-4 78-79 2.488657056658189E-4 80-81 5.332836549981833E-4 82-83 1.7776121833272776E-4 84-85 3.9107468033200107E-4 86-87 3.555224366654555E-4 88-89 0.0013154330156621855 90-91 0.002133134619992733 92-93 4.266269239985466E-4 94-95 9.599105789967299E-4 96-97 0.0013865375029952765 98-99 0.002097582376326188 100-101 9.599105789967299E-4 102-103 0.0010310150663298211 104-105 9.243583353301843E-4 106-107 5.332836549981833E-4 108-109 0.0010665673099963665 110-111 0.0011376717973294577 112-113 0.012265524064958216 114-115 0.020726958057596057 116-117 0.018913793630602232 118-119 0.011092300023962212 120-121 0.006043881423312744 122-123 0.005901672448646562 124-125 0.0055817022556476515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1406381.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.271540942927118 #Duplication Level Percentage of deduplicated Percentage of total 1 44.789240497494006 10.423146440400409 2 14.821774139731398 6.89851047479155 3 10.663808745723292 7.444897855009315 4 7.792871804949685 7.254085410874757 5 5.400941376456847 6.284411418628231 6 3.8693785038781603 5.402784016600961 7 2.6591899208074183 4.331841298196226 8 1.8639674543606026 3.4701915944349118 9 1.3680083605904745 2.8652096316373044 >10 6.077782072477981 24.214396050144895 >50 0.43507834660066713 6.9956138850235075 >100 0.24621933669836593 10.416633196414423 >500 0.00834118121691038 1.2919960265054935 >1k 0.0024714611013067793 0.8989548892896717 >5k 9.267979129900421E-4 1.8073278120483889 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9855 0.7007347226676128 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7796 0.5543305832487783 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7488 0.5324304011501862 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2251 0.16005620098678808 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2141 0.15223470738014805 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1970 0.14007584004618948 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1407 0.10004401367765918 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.11044873330911E-5 0.0 0.0 0.0 0.0 6 7.11044873330911E-5 0.0 0.0 0.0 0.0 7 4.266269239985466E-4 0.0 0.0 0.0 0.0 8 4.266269239985466E-4 0.0 0.0 0.0 0.0 9 4.266269239985466E-4 0.0 0.0 0.0 0.0 10-11 4.266269239985466E-4 0.0 0.0 0.0 0.0 12-13 4.266269239985466E-4 0.0 0.0 0.0 0.0 14-15 4.266269239985466E-4 0.0 0.0 0.0 0.0 16-17 4.266269239985466E-4 0.0 0.0 3.555224366654555E-5 0.0 18-19 4.266269239985466E-4 0.0 0.0 7.11044873330911E-5 0.0 20-21 4.266269239985466E-4 0.0 0.0 7.11044873330911E-5 0.0 22-23 6.399403859978199E-4 0.0 0.0 1.0665673099963667E-4 0.0 24-25 7.821493606640021E-4 0.0 0.0 4.6217916766509216E-4 0.0 26-27 7.821493606640021E-4 0.0 0.0 7.821493606640021E-4 0.0 28-29 8.177016043305477E-4 0.0 0.0 0.0012798807719956399 0.0 30-31 8.532538479970932E-4 0.0 0.0 0.004621791676650921 0.0 32-33 8.532538479970932E-4 0.0 0.0 0.013225434643954946 0.0 34-35 0.0013154330156621855 0.0 0.0 0.02307340613958806 0.0 36-37 0.0014931942339949133 0.0 0.0 0.04124060265319284 0.0 38-39 0.0014931942339949133 0.0 0.0 0.07170887547542237 0.0 40-41 0.0014931942339949133 0.0 0.0 0.10367034253164684 0.0 42-43 0.0015998509649945499 0.0 0.0 0.1422089746661822 0.0 44-45 0.0017065076959941864 0.0 0.0 0.19009784688501907 0.0 46-47 0.0017065076959941864 0.0 0.0 0.24847463098548686 0.0 48-49 0.0017065076959941864 0.0 0.0 0.3089489974622809 0.0 50-51 0.0017065076959941864 0.0 0.0 0.3705610357364043 0.0 52-53 0.0017065076959941864 0.0 0.0 0.44298095608515753 0.0 54-55 0.0017065076959941864 0.0 0.0 0.5290884902455308 0.0 56-57 0.0023464480819920066 0.0 0.0 0.634536444960505 0.0 58-59 0.0023464480819920066 0.0 0.0 0.7584004618947497 0.0 60-61 0.0023464480819920066 0.0 0.0 0.8802024486963348 0.0 62-63 0.0023464480819920066 0.0 0.0 1.0096481678862272 0.0 64-65 0.0023464480819920066 0.0 0.0 1.1540258294160686 0.0 66-67 0.002630866031324371 0.0 0.0 1.3300449878091356 0.0 68-69 0.002844179493323644 0.0 0.0 1.5378478520400942 0.0 70-71 0.002844179493323644 0.0 0.0 1.7547876428933553 0.0 72-73 0.002844179493323644 0.0 0.0 1.967070089826299 0.0 74-75 0.002844179493323644 0.0 0.0 2.19471110602319 0.0 76-77 0.002844179493323644 0.0 0.0 2.468356725524591 0.0 78-79 0.002986388467989826 0.0 0.0 2.7970727704654714 0.0 80-81 0.0031285974426560085 0.0 0.0 3.123833442004692 0.0 82-83 0.0031285974426560085 0.0 0.0 3.4635706824822003 0.0 84-85 0.0031285974426560085 0.0 0.0 3.8295454787856205 0.0 86-87 0.0031285974426560085 0.0 0.0 4.227339533170598 0.0 88-89 0.0031285974426560085 0.0 0.0 4.662498995649116 0.0 90-91 0.0031997019299890997 0.0 0.0 5.134170612373176 0.0 92-93 0.0031997019299890997 0.0 0.0 5.626213664718167 0.0 94-95 0.0031997019299890997 0.0 0.0 6.123234031176473 0.0 96-97 0.0031997019299890997 0.0 0.0 6.686737093291221 0.0 98-99 0.0031997019299890997 0.0 0.0 7.296849146852809 0.0 100-101 0.0031997019299890997 0.0 0.0 7.9613561332242115 0.0 102-103 0.0031997019299890997 0.0 0.0 8.606380490066348 0.0 104-105 0.0035552243666545553 0.0 0.0 9.26687007290343 0.0 106-107 0.0035552243666545553 0.0 0.0 9.957365749395079 0.0 108-109 0.0036974333413207373 0.0 0.0 10.678827430120288 0.0 110-111 0.0038396423159869197 0.0 0.0 11.461438969951955 0.0 112-113 0.0038396423159869197 0.0 0.0 12.250236600181601 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4570 0.0 91.37891 1 GTATCAA 7450 0.0 56.10391 2 GTACATG 10480 0.0 46.04345 1 TACATGG 10975 0.0 43.563553 2 ACATGGG 10700 0.0 43.458977 3 CATGGGA 7505 0.0 41.650562 4 ATCAACG 10885 0.0 40.20418 4 TCAACGC 10910 0.0 40.166622 5 CAACGCA 10980 0.0 39.989124 6 TATCACG 75 8.156838E-5 39.69365 2 AGCGTAC 60 0.0013042442 39.69365 3 AACGCAG 11230 0.0 39.0989 7 TATAACG 125 2.9582225E-8 38.105904 2 TATCAAC 11660 0.0 37.327686 3 ATGGGAT 3340 0.0 35.652977 5 CATGGGG 2950 0.0 34.109627 4 ATCACGC 105 1.4770279E-5 34.023125 3 ATGGGAG 2895 0.0 33.317986 5 ACGCAGA 13165 0.0 33.171345 8 CGCAGAG 13295 0.0 32.66799 9 >>END_MODULE