FastQCFastQC Report
Thu 26 May 2016
SRR936206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18312
Sequences flagged as poor quality0
Sequence length125
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4562.490170380078637No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.436871996505024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA800.436871996505024No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.40956749672346004No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.36041939711664484No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT590.3221930974224552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.28942769768457843No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.25666229794670165No Hit
GTACATGGGCATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCC470.25666229794670165No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.2512013979903888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.2402795980777632No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.22935779816513763No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.218435998252512No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG370.2020532983835736No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.19659239842726078No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC350.191131498470948No Hit
TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.18567059851463522No Hit
AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.18567059851463522No Hit
GTACTTAGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAC320.17474879860200962No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1529051987767584No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT280.1529051987767584No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT270.1474442988204456No Hit
GTACATGGGTACACCATCTAAGGCCAGGCACAGTGACTCCCGCCTGTAAT270.1474442988204456No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT270.1474442988204456No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA260.1419833988641328No Hit
GTACATGGGCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCA260.1419833988641328No Hit
AAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.13652249890782No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.13652249890782No Hit
AAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.13652249890782No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC240.1310615989515072No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG230.1256006989951944No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT220.1201397990388816No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG210.11467889908256881No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC210.11467889908256881No Hit
GTACATGGGCCATGATTACGAATTCTTAGTGGTGGTGGTGATGGTGATCA200.109217999126256No Hit
ACGCAGAGAACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA200.109217999126256No Hit
GAGAACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA190.1037570991699432No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG190.1037570991699432No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCATC152.6589108E-4119.07
GCATCTA152.6589108E-4119.09
GTACAAG205.544909E-6118.999991
GGCATCT208.34783E-489.249998
CAGGGCC208.34783E-489.249994
ACATAAA208.34783E-489.249993
TGGGCAT208.34783E-489.249996
CATGAAA304.1529485E-579.3333364
CACAAAG401.904822E-674.3759
CACAACG250.002024553371.47
AACATGA358.914442E-568.02
ACATGAA551.6345803E-764.909093
CAATGAA150.00420390159.516-17
TAGTGTG150.00420390159.546-47
CCCATTC150.00420390159.568-69
TATAGTG150.00420390159.544-45
CACAGAA300.004170332659.57
ATGAAAA401.7260696E-459.4999965
GGAAACA902.1827873E-1159.4999961
ACAACAC1150.056.9130445
Produced by FastQC (version 0.11.5)