##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936206_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18312 Sequences flagged as poor quality 0 Sequence length 125 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.609927916120576 27.0 10.0 31.0 2.0 34.0 2 22.315148536478812 27.0 12.0 31.0 2.0 34.0 3 22.82557885539537 27.0 12.0 31.0 2.0 34.0 4 24.844637396242902 31.0 12.0 37.0 2.0 37.0 5 27.090814766273482 32.0 23.0 37.0 2.0 37.0 6 25.41464613368283 31.0 13.0 37.0 2.0 37.0 7 26.01119484491044 32.0 13.0 37.0 2.0 37.0 8 24.76119484491044 31.0 8.0 37.0 2.0 37.0 9 24.484436435124508 31.0 8.0 39.0 2.0 39.0 10-11 25.59998907820009 31.0 9.5 39.0 2.0 39.0 12-13 25.721821756225427 31.5 10.0 39.0 2.0 39.0 14-15 26.461227610310182 32.0 8.0 40.0 2.0 41.0 16-17 26.779761904761905 32.0 11.5 40.0 2.0 41.0 18-19 27.050595238095237 32.0 14.0 40.0 2.0 41.0 20-21 27.278396679772825 32.0 17.0 40.0 2.0 41.0 22-23 27.385075360419396 31.5 19.0 40.0 2.0 41.0 24-25 27.455002184359984 31.0 23.0 40.0 2.0 41.0 26-27 26.88059742245522 31.0 18.5 40.0 2.0 41.0 28-29 26.244102228047183 30.0 5.0 39.5 2.0 41.0 30-31 25.587729357798167 30.0 2.0 39.0 2.0 41.0 32-33 24.930591961555265 30.0 2.0 39.0 2.0 41.0 34-35 24.305373525557012 30.0 2.0 39.0 2.0 41.0 36-37 23.653860856269112 30.0 2.0 39.0 2.0 41.0 38-39 23.044451725644386 30.0 2.0 39.0 2.0 41.0 40-41 22.86680865006553 30.0 2.0 38.5 2.0 41.0 42-43 22.477337265181305 29.0 2.0 39.0 2.0 41.0 44-45 22.316704892966364 29.0 2.0 39.0 2.0 41.0 46-47 22.141710353866316 29.0 2.0 38.0 2.0 41.0 48-49 21.837155963302752 29.0 2.0 38.5 2.0 41.0 50-51 21.562909567496725 28.5 2.0 38.0 2.0 41.0 52-53 21.34936107470511 28.5 2.0 38.0 2.0 41.0 54-55 21.131853429445172 28.0 2.0 38.0 2.0 40.5 56-57 20.874044342507645 28.0 2.0 38.0 2.0 40.0 58-59 20.633327872433377 28.0 2.0 37.0 2.0 40.0 60-61 20.351135867190912 27.5 2.0 37.0 2.0 40.0 62-63 20.009938837920487 27.0 2.0 36.0 2.0 40.0 64-65 19.679144823066842 26.0 2.0 36.0 2.0 40.0 66-67 19.36574377457405 25.5 2.0 35.0 2.0 39.5 68-69 19.057202927042376 24.5 2.0 35.0 2.0 39.0 70-71 18.669752075141982 23.0 2.0 35.0 2.0 39.0 72-73 18.32880078636959 21.0 2.0 35.0 2.0 38.5 74-75 17.916448230668415 18.0 2.0 35.0 2.0 37.0 76-77 17.60910878112713 11.5 2.0 35.0 2.0 37.0 78-79 17.333879423328966 7.0 2.0 34.0 2.0 36.5 80-81 17.16330821319353 3.5 2.0 34.0 2.0 36.0 82-83 16.923410878112712 2.0 2.0 34.0 2.0 35.5 84-85 16.92630515508956 2.0 2.0 34.0 2.0 35.0 86-87 16.825633464394933 2.0 2.0 34.0 2.0 35.0 88-89 16.671827217125383 2.0 2.0 34.0 2.0 35.0 90-91 16.471985583224118 2.0 2.0 34.0 2.0 35.0 92-93 16.382699868938403 2.0 2.0 34.0 2.0 35.0 94-95 16.25109217999126 2.0 2.0 34.0 2.0 35.0 96-97 16.16494648318043 2.0 2.0 34.0 2.0 35.0 98-99 16.022389689820884 2.0 2.0 34.0 2.0 35.0 100-101 15.906290956749672 2.0 2.0 34.0 2.0 35.0 102-103 15.333169506334645 2.0 2.0 32.5 2.0 34.5 104-105 15.640126692878987 2.0 2.0 33.5 2.0 35.0 106-107 15.704865661861074 2.0 2.0 34.0 2.0 35.0 108-109 15.661970292704236 2.0 2.0 34.0 2.0 35.0 110-111 15.554417868064657 2.0 2.0 34.0 2.0 35.0 112-113 15.500764525993883 2.0 2.0 34.0 2.0 35.0 114-115 15.321865443425075 2.0 2.0 34.0 2.0 35.0 116-117 15.160741590214066 2.0 2.0 34.0 2.0 35.0 118-119 14.99565858453473 2.0 2.0 34.0 2.0 35.0 120-121 14.791885102664919 2.0 2.0 34.0 2.0 35.0 122-123 14.541257099169943 2.0 2.0 33.5 2.0 35.0 124-125 13.472285932721713 2.0 2.0 31.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.388553528599367 1101 2 2.895786451992766 1101 3 -0.3149684844430958 1101 4 2.9383085620526153 1101 5 2.7191933513782587 1101 6 0.9418682557125706 1101 7 1.20322773926722 1101 8 0.4692670672213879 1101 9 1.3130350121814658 1101 10-11 2.211653930809373 1101 12-13 2.2907766493293806 1101 14-15 1.6781719155803572 1101 16-17 1.3292615542594426 1101 18-19 0.23584615746649717 1101 20-21 0.16514503341002396 1101 22-23 1.7146792962754667 1101 24-25 2.2983360711007847 1101 26-27 3.0066811548268433 1101 28-29 2.779486712264518 1101 30-31 3.09370435135547 1101 32-33 3.6935168788146626 1101 34-35 4.909089085623599 1101 36-37 4.173927683707181 1101 38-39 5.654510202339953 1101 40-41 3.5966413897487293 1101 42-43 4.561302851749211 1101 44-45 4.81982171892961 1101 46-47 4.558827898837304 1101 48-49 3.0637344546043295 1101 50-51 2.713850188281679 1101 52-53 2.511876098348168 1101 54-55 3.0661171055603447 1101 56-57 1.8093257599910757 1101 58-59 1.4187640612519807 1101 60-61 1.697779162248274 1101 62-63 1.2509589107746812 1101 64-65 1.1490715757625125 1101 66-67 0.9885228106351143 1101 68-69 0.536716957981124 1101 70-71 0.4419697428363101 1101 72-73 1.7585088583986277 1101 74-75 2.2552961791218458 1101 76-77 2.2506180772006097 1101 78-79 2.8642697212374024 1101 80-81 2.267998660136257 1101 82-83 2.7003167951145386 1101 84-85 1.4673996363719226 1101 86-87 1.4848563677667812 1101 88-89 2.119124340448014 1101 90-91 1.9618208240614798 1101 92-93 1.93668271745479 1101 94-95 1.6678336662296473 1101 96-97 2.1059441197782185 1101 98-99 1.5008839411643073 1101 100-101 0.6686325415727694 1101 102-103 0.6630677748823217 1101 104-105 0.7209577903255884 1101 106-107 1.292106170636096 1101 108-109 1.0360881527022219 1101 110-111 1.2473321325749502 1101 112-113 1.1990795143683979 1101 114-115 1.1043178601128432 1101 116-117 0.9195818079925626 1101 118-119 0.8557812588897225 1101 120-121 0.1803579434999758 1101 122-123 2.2564492590008705 1101 124-125 1.7672136777781837 1102 1 3.4000477814729315 1102 2 3.6505757240234153 1102 3 4.140970212874912 1102 4 4.297184679549421 1102 5 3.6106365186834957 1102 6 3.8269257269769383 1102 7 3.9209799742608347 1102 8 5.038871154066854 1102 9 4.445857489831532 1102 10-11 4.583940009992006 1102 12-13 3.755655321104605 1102 14-15 4.128363486461584 1102 16-17 4.820321579802226 1102 18-19 3.6790134627027697 1102 20-21 4.860547332260605 1102 22-23 4.711486448255027 1102 24-25 4.3819886892361595 1102 26-27 5.013705420471798 1102 28-29 4.288426686721735 1102 30-31 5.091150072939122 1102 32-33 6.091345742160769 1102 34-35 5.92505332572577 1102 36-37 6.032165231599901 1102 38-39 5.190270100168817 1102 40-41 4.660498350157418 1102 42-43 4.909323285976541 1102 44-45 4.897727210628204 1102 46-47 4.302122917994392 1102 48-49 4.2240154252301245 1102 50-51 4.452036650606072 1102 52-53 3.5923358684631097 1102 54-55 3.8317620608604734 1102 56-57 4.177141851945098 1102 58-59 4.393859846692592 1102 60-61 4.969809813589272 1102 62-63 4.985313955474549 1102 64-65 5.633107335660341 1102 66-67 5.279071980494628 1102 68-69 5.449871491825313 1102 70-71 5.530092348200295 1102 72-73 5.286605920978445 1102 74-75 5.567556715520315 1102 76-77 5.445381806447099 1102 78-79 5.514972147801895 1102 80-81 4.7986500011324225 1102 82-83 4.92253901733676 1102 84-85 5.386939866256977 1102 86-87 3.8973723320068814 1102 88-89 4.352202245939711 1102 90-91 4.390301028403751 1102 92-93 4.098240827288766 1102 94-95 4.581626769677925 1102 96-97 4.929060339446163 1102 98-99 4.876362868367369 1102 100-101 4.434277496872898 1102 102-103 3.869964326606457 1102 104-105 3.844201723914349 1102 106-107 3.735273475872365 1102 108-109 3.7742746150266147 1102 110-111 3.9248544825110976 1102 112-113 4.3523362193492385 1102 114-115 3.963832547213734 1102 116-117 3.348700581938923 1102 118-119 3.44581957306597 1102 120-121 4.355326015019774 1102 122-123 5.01570851826013 1102 124-125 5.8380949038318235 1103 1 1.2165105178466789 1103 2 -0.9500916842079512 1103 3 0.6025942395712391 1103 4 0.8033025883369618 1103 5 -2.19359040011517 1103 6 0.8235886858200949 1103 7 0.9332157918359201 1103 8 -0.14244141545483657 1103 9 0.7550899703654572 1103 10-11 0.27915691766719775 1103 12-13 0.870227067489477 1103 14-15 -0.5279212743838002 1103 16-17 -1.3487551721443793 1103 18-19 -0.8220988843495114 1103 20-21 -2.2595861493856724 1103 22-23 -2.597746032278895 1103 24-25 -1.6580558046459295 1103 26-27 0.5198233292593386 1103 28-29 0.5704066644747954 1103 30-31 0.12118344335068798 1103 32-33 0.5991321715267297 1103 34-35 1.7615383090405636 1103 36-37 1.8764366442806555 1103 38-39 2.463351301503632 1103 40-41 2.7161157027714324 1103 42-43 3.438244309335829 1103 44-45 3.1107416711398876 1103 46-47 1.3043476120989546 1103 48-49 0.34748594803324195 1103 50-51 0.304650666178933 1103 52-53 -0.031134654340007728 1103 54-55 0.5631302477347759 1103 56-57 -0.4057280034497843 1103 58-59 -0.6439599530849378 1103 60-61 -0.22874413524276704 1103 62-63 -1.6654090701094297 1103 64-65 -1.0098292605576802 1103 66-67 -0.854965839305148 1103 68-69 0.0032641503347683454 1103 70-71 0.026199133517312845 1103 72-73 0.08527110451571218 1103 74-75 0.14597718270607274 1103 76-77 -0.40111430033821804 1103 78-79 0.16235813222903417 1103 80-81 -0.6423956050967199 1103 82-83 -1.1419771116954962 1103 84-85 -0.5218476754560335 1103 86-87 -0.7433395701065777 1103 88-89 0.24875397007764377 1103 90-91 0.6667192708954097 1103 92-93 -0.8094343673719706 1103 94-95 -0.057972785383256564 1103 96-97 -0.5798384369720821 1103 98-99 -0.3127361305202818 1103 100-101 -0.2053220581882833 1103 102-103 -0.5788677089886463 1103 104-105 -0.8549083984438255 1103 106-107 -0.6690646776315283 1103 108-109 -0.5666597564972999 1103 110-111 -1.3398841159316177 1103 112-113 -0.8045047150222846 1103 114-115 -0.7552998220854867 1103 116-117 0.20632015924704206 1103 118-119 0.0403690725097956 1103 120-121 -0.2826050194629879 1103 122-123 -0.5810656752882579 1103 124-125 -1.0517827379924256 1104 1 -3.244779804733966 1104 2 -2.826435770229459 1104 3 -2.2705240399986515 1104 4 -5.057987734243682 1104 5 -3.721547389362481 1104 6 -3.7247984109540972 1104 7 -1.9560315199920382 1104 8 -2.5714736735193533 1104 9 -4.527705728559273 1104 10-11 -4.540197921042477 1104 12-13 -5.00181594326321 1104 14-15 -5.894050306641866 1104 16-17 -6.117034742036854 1104 18-19 -7.021561249940909 1104 20-21 -5.6181882999233075 1104 22-23 -4.614950333354162 1104 24-25 -4.255367632602919 1104 26-27 -4.024800326654638 1104 28-29 -3.274216991439186 1104 30-31 -2.7599993523482347 1104 32-33 -3.721297936000152 1104 34-35 -4.4760960995615875 1104 36-37 -4.864961205181711 1104 38-39 -5.043637945808197 1104 40-41 -5.444099350992012 1104 42-43 -5.642400851954491 1104 44-45 -7.000548651440759 1104 46-47 -6.763394323384919 1104 48-49 -7.712191471321599 1104 50-51 -7.9157794413479525 1104 52-53 -7.488123901651836 1104 54-55 -8.032031042587803 1104 56-57 -8.090674240008926 1104 58-59 -8.159013716525797 1104 60-61 -9.807776393307282 1104 62-63 -9.600892941077174 1104 64-65 -9.799076572385635 1104 66-67 -8.881847559735256 1104 68-69 -8.472542301278136 1104 70-71 -8.163585812719244 1104 72-73 -8.750750400860635 1104 74-75 -8.583592709767043 1104 76-77 -8.24197451539198 1104 78-79 -8.383878426910748 1104 80-81 -8.0375568954193 1104 82-83 -8.22746098266324 1104 84-85 -8.18260036362808 1104 86-87 -7.983662150751737 1104 88-89 -7.7012460299223555 1104 90-91 -7.208549546308891 1104 92-93 -7.75405802328595 1104 94-95 -8.115499667103686 1104 96-97 -8.158870695036597 1104 98-99 -8.021338281057917 1104 100-101 -7.996182273242045 1104 102-103 -7.536932225117678 1104 104-105 -7.70867183930404 1104 106-107 -7.631967903437982 1104 108-109 -7.59354147692741 1104 110-111 -7.711927126684307 1104 112-113 -7.345364930076046 1104 114-115 -7.266052510257529 1104 116-117 -7.2915293031185495 1104 118-119 -7.621996518888054 1104 120-121 -7.749271686129655 1104 122-123 -7.450958148406537 1104 124-125 -6.605008544444038 1105 1 -1.0114464714006317 1105 2 -1.626435770229456 1105 3 -2.17052403999865 1105 4 -1.5246544009103502 1105 5 -3.821547389362479 1105 6 -1.85813174428743 1105 7 -1.5560315199920396 1105 8 -0.371473673519354 1105 9 -0.09437239522593899 1105 10-11 -3.656864587709144 1105 12-13 -3.0684826099298803 1105 14-15 -3.1440503066418657 1105 16-17 -4.583701408703519 1105 18-19 -2.621561249940907 1105 20-21 -1.8181882999233103 1105 22-23 -1.6816170000208324 1105 24-25 -0.4720342992695876 1105 26-27 -0.10813365998797053 1105 28-29 0.6924496752274791 1105 30-31 0.5733339809850975 1105 32-33 0.36203539733318024 1105 34-35 -1.3927627662282518 1105 36-37 -2.5649612051817066 1105 38-39 -2.0436379458081966 1105 40-41 -2.9107660176586734 1105 42-43 -2.7257341852878234 1105 44-45 -3.250548651440763 1105 46-47 -2.180060990051583 1105 48-49 -2.0788581379882665 1105 50-51 -2.5824461080146186 1105 52-53 -2.1381239016518343 1105 54-55 -1.9820310425878027 1105 56-57 -1.7740075733422564 1105 58-59 -1.5423470498591314 1105 60-61 -1.4077763933072802 1105 62-63 -1.6842262744105057 1105 64-65 -2.732409905718967 1105 66-67 -2.2151808930685917 1105 68-69 -2.2392089679448013 1105 70-71 -2.296919146052577 1105 72-73 -2.0840837341939675 1105 74-75 -1.916926043100375 1105 76-77 -1.7919745153919813 1105 78-79 -1.4005450935774135 1105 80-81 -1.3708902287526321 1105 82-83 -1.0441276493299085 1105 84-85 -0.9826003636280767 1105 86-87 -1.0003288174184028 1105 88-89 -1.0012460299223562 1105 90-91 -1.8085495463088925 1105 92-93 -1.8040580232859487 1105 94-95 -1.898833000437019 1105 96-97 -1.8255373617032653 1105 98-99 -2.6880049477245826 1105 100-101 -3.52951560657538 1105 102-103 -2.986932225117677 1105 104-105 -3.442005172637373 1105 106-107 -3.0153012367713146 1105 108-109 -2.9268748102607436 1105 110-111 -2.7952604600176407 1105 112-113 -3.112031596742714 1105 114-115 -3.216052510257528 1105 116-117 -3.5081959697852163 1105 118-119 -2.9719965188880533 1105 120-121 -1.815938352796321 1105 122-123 -1.8509581484065372 1105 124-125 -1.2883418777773699 1106 1 2.700381485588615 1106 2 0.6305534770823691 1106 3 4.021949078280919 1106 4 4.835560652853093 1106 5 1.51608701923967 1106 6 2.081653201949127 1106 7 3.0299899853843044 1106 8 2.7607843909967755 1106 9 2.851864163913845 1106 10-11 3.0533504660542974 1106 12-13 2.1766786803927047 1106 14-15 1.9559496933581357 1106 16-17 1.006083537533037 1106 18-19 2.2746753091988765 1106 20-21 1.1758977215820714 1106 22-23 1.7248346128823933 1106 24-25 -1.883862256258837 1106 26-27 3.1488555873238546 1106 28-29 4.054277632216731 1106 30-31 3.830860862705528 1106 32-33 0.8571966876557617 1106 34-35 3.7615383090405636 1106 36-37 4.102243095893559 1106 38-39 4.640770656342344 1106 40-41 5.3612769930940125 1106 42-43 5.825341083529381 1106 44-45 5.53009650984956 1106 46-47 5.207573418550567 1106 48-49 5.105550464162274 1106 50-51 3.8691667952111892 1106 52-53 3.372091152111608 1106 54-55 3.708291538057356 1106 56-57 4.61040102880828 1106 58-59 4.614104563044094 1106 60-61 3.045449413144329 1106 62-63 2.5442683492454066 1106 64-65 2.0546868684745796 1106 66-67 1.6127760961787203 1106 68-69 2.1322964083992844 1106 70-71 1.4132959077108644 1106 72-73 2.32720658838668 1106 74-75 1.6621062149641403 1106 76-77 0.7440469899843656 1106 78-79 0.9526807128741979 1106 80-81 1.599539878774248 1106 82-83 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-0.5080180242643966 2215 120-121 -0.7180888904307299 2215 122-123 -0.9520334172237419 2215 124-125 -1.8098472541214576 2216 1 3.944109084154924 2216 2 2.9328234890298006 2216 3 4.722068552593942 2216 4 3.6049752287192796 2216 5 3.830304462489373 2216 6 4.867794181638494 2216 7 3.092116628156109 2216 8 4.17297077092509 2216 9 1.2759979751444313 2216 10-11 4.470913190068632 2216 12-13 3.7167025752553045 2216 14-15 4.0855793229877655 2216 16-17 3.718150443148332 2216 18-19 6.198809120429463 2216 20-21 5.035515403780398 2216 22-23 5.6220867036828714 2216 24-25 5.946484219248937 2216 26-27 6.395570043715733 2216 28-29 5.501708934486743 2216 30-31 6.241852499503615 2216 32-33 6.286109471407258 2216 34-35 6.649829826364339 2216 36-37 5.562816572596066 2216 38-39 5.358213906043655 2216 40-41 5.337382130489473 2216 42-43 6.283525073971433 2216 44-45 6.7272291263370185 2216 46-47 5.614383454392861 2216 48-49 4.119290010159883 2216 50-51 3.8619983364298243 2216 52-53 3.530394616866687 2216 54-55 3.288339327782566 2216 56-57 2.661177611842927 2216 58-59 2.159504801992721 2216 60-61 0.3274087918779003 2216 62-63 2.158366318182086 2216 64-65 2.2046271313180696 2216 66-67 2.599633921746225 2216 68-69 2.1107910320551966 2216 70-71 1.3678956687622374 2216 72-73 0.6288792287689944 2216 74-75 0.829370253195922 2216 76-77 1.4172847438672775 2216 78-79 -0.00610064913297137 2216 80-81 -0.32459393245633805 2216 82-83 0.6632797580775005 2216 84-85 1.0599922289645143 2216 86-87 1.3552267381371514 2216 88-89 1.0080132293369033 2216 90-91 0.3136726759133275 2216 92-93 0.4737197544918281 2216 94-95 0.5937595921555712 2216 96-97 0.2726107864448828 2216 98-99 0.5008839411643073 2216 100-101 -0.40544153250130677 2216 102-103 -1.299895188080642 2216 104-105 -2.4271903578225587 2216 106-107 -2.1153012367713124 2216 108-109 -1.630578513964446 2216 110-111 -0.6045197192768992 2216 112-113 -0.24536493007604854 2216 114-115 -0.06234880655382469 2216 116-117 -0.5433811549704011 2216 118-119 -1.8108854077769418 2216 120-121 -1.8566790935370605 2216 122-123 -2.447254444702832 2216 124-125 -2.28834187777737 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 2294.0 3 312.0 4 338.0 5 626.0 6 1430.0 7 1096.0 8 469.0 9 404.0 10 363.0 11 288.0 12 312.0 13 311.0 14 256.0 15 224.0 16 146.0 17 128.0 18 123.0 19 100.0 20 94.0 21 106.0 22 101.0 23 143.0 24 271.0 25 273.0 26 272.0 27 222.0 28 242.0 29 296.0 30 290.0 31 381.0 32 449.0 33 543.0 34 715.0 35 1011.0 36 1376.0 37 1697.0 38 608.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.494975972040194 23.115989515072084 10.129969418960243 13.25906509392748 2 16.355395369156835 37.05766710353866 28.090869375273048 18.496068152031455 3 18.52337265181302 48.935124508519 18.266710353866316 14.27479248580166 4 8.972258628221931 31.30187854958497 22.362385321100916 37.36347750109218 5 7.967888154661133 60.07318005570422 20.4358036153132 11.523128174321446 6 20.616023155480313 46.256348642892256 14.50494238435913 18.62268581726831 7 20.413958822565668 52.17082627928568 12.790126153678116 14.625088744470538 8 20.774397902899896 50.663535579706185 13.161487630386107 15.40057888700781 9 20.523182786303316 35.5960897820982 10.409043744197477 33.47168368740102 10-11 17.03893834307247 47.40046966304407 14.974605428430998 20.585986565452462 12-13 19.966685781382267 47.784058327189314 15.338193932443133 16.911061958985282 14-15 21.050763223287188 46.4815269927091 15.42011414215887 17.047595641844843 16-17 18.257782632441288 46.247951938831235 17.905516111414528 17.588749317312942 18-19 18.35608956854178 43.039322774440194 21.17422173675587 17.430365920262155 20-21 21.329874385581647 40.92572364827963 20.854724194429274 16.88967777170945 22-23 20.346533300538383 42.38200650433166 19.36815063813506 17.90330955699489 24-25 19.492080830147458 42.57509557618788 18.722009830693608 19.210813762971053 26-27 19.563080283997817 41.469142545057345 20.382304751501913 18.58547241944293 28-29 18.582234237186313 44.7366265257639 19.56527675377516 17.115862483274626 30-31 19.07323466768609 45.64196384686801 19.430943148926875 15.853858336519034 32-33 16.810027033669208 46.410529477622134 20.632970153736927 16.146473334971738 34-35 16.036808475779583 47.83190431980777 19.501938725356343 16.629348479056304 36-37 16.781803784507005 48.33847582120525 18.177101821259864 16.702618573027877 38-39 16.88602479383977 47.77183113975207 18.584457429960132 16.757686636448035 40-41 15.55391715136124 49.53168946779171 18.06067556866279 16.853717812184264 42-43 16.62889441061628 50.10239467001612 18.237173361002647 15.031537558364963 44-45 16.142861043606477 50.09693362094858 18.34912486688693 15.41108046855801 46-47 15.730981377314182 49.65321391513298 18.79744415924854 15.818360548304296 48-49 14.76121562952243 49.490757174453215 18.7122846298774 17.035742566146958 50-51 14.917670189235684 47.87690123153382 18.1507877993501 19.054640779880398 52-53 15.275388438327736 47.69121542284482 17.339777723164303 19.693618415663146 54-55 15.433346076129103 46.82977445251488 17.841734476544154 19.89514499481186 56-57 16.481896128010483 46.32461362022828 17.544099175359072 19.64939107640216 58-59 16.543796417649627 45.46745303626037 17.491262560069902 20.497487986020097 60-61 17.541368576265633 44.86647370433073 17.60963355360166 19.982524165801976 62-63 17.719341361513884 44.34614019278556 17.768493487343328 20.166024958357227 64-65 18.983124897602536 43.21992245098575 18.442466277101197 19.35448637431052 66-67 20.173666102342853 44.06367757085904 17.063513734913442 18.69914259188466 68-69 20.54663608562691 44.32339449541284 17.480340760157272 17.64962865880297 70-71 20.19222892717691 44.136198563744095 18.149796575922235 17.52177593315676 72-73 21.31389252948886 44.09130624726955 17.96909130624727 16.62570991699432 74-75 21.19102228047182 42.21002621231979 19.476299694189603 17.122651813018784 76-77 21.72346002621232 40.57994757536042 19.855832241153344 17.84076015727392 78-79 22.370576671035387 39.924639580602886 20.688619484491046 17.016164263870685 80-81 22.010157273918743 38.34643949322849 21.633355176933158 18.01004805591962 82-83 22.354193971166445 38.01605504587156 20.931629532546964 18.69812145041503 84-85 21.947356924421143 37.691131498470945 21.72346002621232 18.638051550895586 86-87 22.70096111839231 37.303407601572744 21.3302752293578 18.66535605067715 88-89 21.870904325032768 37.96690694626474 21.087265181301877 19.074923547400612 90-91 21.977391874180867 38.19080384447357 20.84425513324596 18.987549148099607 92-93 20.784185233726518 39.09185233726518 20.478374836173003 19.645587592835298 94-95 19.8585626911315 39.971057230231544 20.232634338138926 19.937745740498034 96-97 19.732961992136307 41.906946264744434 19.621013543031893 18.739078200087373 98-99 18.5648363050542 43.84403243863146 19.039947573928952 18.551183682385386 100-101 16.673038064551363 46.75331767789853 17.55775217082628 19.015892086723827 102-103 16.014089121887288 49.44298820445609 17.349279161205764 17.19364351245085 104-105 15.089290590355525 51.5345966905139 16.798645622849655 16.577467096280923 106-107 13.999399284602573 52.93394861152827 17.106736204024795 15.95991589984436 108-109 14.706605138847173 53.77495016792726 16.79545640717582 14.722988286049752 110-111 14.248266069575665 55.05706952105292 15.75009557096827 14.944568838403146 112-113 14.444080384447359 55.346221057230224 15.446155526430754 14.763543031891654 114-115 14.74442988204456 54.49978156400175 15.478920926168632 15.276867627785059 116-117 15.230449978156399 53.896352118829185 15.852992573176058 15.020205329838356 118-119 15.642747924858016 52.67857142857143 16.082350371341196 15.59633027522936 120-121 17.12538226299694 51.504477937964175 16.371778069025776 14.998361730013107 122-123 16.912407164700742 49.98907820008737 16.950633464394933 16.147881170816948 124-125 17.941786806465707 49.754259501965926 15.877566622979467 16.426387068588905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 261.0 1 239.5 2 239.5 3 254.5 4 212.0 5 148.5 6 109.0 7 99.5 8 86.5 9 82.0 10 79.5 11 68.0 12 69.0 13 68.5 14 66.5 15 67.5 16 69.0 17 78.5 18 93.5 19 102.0 20 106.5 21 116.5 22 147.0 23 174.0 24 196.0 25 251.0 26 312.5 27 361.0 28 388.5 29 438.0 30 483.5 31 502.5 32 501.0 33 492.0 34 519.5 35 556.5 36 566.0 37 470.5 38 423.0 39 446.0 40 401.5 41 356.0 42 335.0 43 323.5 44 338.0 45 336.5 46 318.0 47 351.0 48 393.0 49 431.5 50 562.0 51 552.5 52 389.5 53 284.5 54 290.5 55 346.0 56 361.5 57 374.0 58 327.0 59 226.5 60 164.0 61 140.0 62 117.0 63 100.0 64 78.5 65 71.0 66 65.0 67 53.0 68 52.0 69 61.0 70 57.5 71 46.0 72 37.0 73 29.5 74 26.0 75 25.0 76 24.5 77 16.5 78 8.5 79 8.0 80 6.5 81 3.5 82 2.0 83 1.0 84 1.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.005460899956312801 6 0.005460899956312801 7 0.005460899956312801 8 0.005460899956312801 9 0.005460899956312801 10-11 0.005460899956312801 12-13 0.0081913499344692 14-15 0.0081913499344692 16-17 0.010921799912625601 18-19 0.010921799912625601 20-21 0.010921799912625601 22-23 0.09010484927916121 24-25 0.010921799912625601 26-27 0.010921799912625601 28-29 0.0081913499344692 30-31 0.005460899956312801 32-33 0.0081913499344692 34-35 0.005460899956312801 36-37 0.0027304499781564003 38-39 0.005460899956312801 40-41 0.0081913499344692 42-43 0.0027304499781564003 44-45 0.0027304499781564003 46-47 0.005460899956312801 48-49 0.0027304499781564003 50-51 0.0081913499344692 52-53 0.0081913499344692 54-55 0.005460899956312801 56-57 0.005460899956312801 58-59 0.0 60-61 0.005460899956312801 62-63 0.0081913499344692 64-65 0.005460899956312801 66-67 0.005460899956312801 68-69 0.0 70-71 0.0027304499781564003 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0027304499781564003 100-101 0.005460899956312801 102-103 0.0 104-105 0.005460899956312801 106-107 0.0027304499781564003 108-109 0.0027304499781564003 110-111 0.005460899956312801 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 18312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.05766710353866 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34142907426963 49.858016601135866 2 8.826117564237943 10.954565312363478 3 4.487856388595565 8.355176933158583 4 2.5431186202041536 6.312800349497598 5 1.3199577613516367 4.095674967234601 6 0.7567757831749384 2.8178243774574048 7 0.5279831045406547 2.293577981651376 8 0.34318901795142553 1.7038007863695939 9 0.11439633931714185 0.6389252948885976 >10 0.6775783174938402 8.224115334207077 >50 0.05279831045406547 2.2553516819571864 >100 0.008799718409010912 2.490170380078637 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 456 2.490170380078637 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.436871996505024 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 80 0.436871996505024 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.40956749672346004 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.36041939711664484 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 59 0.3221930974224552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.28942769768457843 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.25666229794670165 No Hit GTACATGGGCATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCC 47 0.25666229794670165 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.2512013979903888 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.2402795980777632 No Hit GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.22935779816513763 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.218435998252512 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 37 0.2020532983835736 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.19659239842726078 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 35 0.191131498470948 No Hit TAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.18567059851463522 No Hit AAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.18567059851463522 No Hit GTACTTAGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAC 32 0.17474879860200962 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.1529051987767584 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT 28 0.1529051987767584 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 27 0.1474442988204456 No Hit GTACATGGGTACACCATCTAAGGCCAGGCACAGTGACTCCCGCCTGTAAT 27 0.1474442988204456 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 27 0.1474442988204456 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 26 0.1419833988641328 No Hit GTACATGGGCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCA 26 0.1419833988641328 No Hit AAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.13652249890782 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.13652249890782 No Hit AAAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.13652249890782 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 24 0.1310615989515072 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 23 0.1256006989951944 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 22 0.1201397990388816 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 21 0.11467889908256881 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 21 0.11467889908256881 No Hit GTACATGGGCCATGATTACGAATTCTTAGTGGTGGTGGTGATGGTGATCA 20 0.109217999126256 No Hit ACGCAGAGAACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 20 0.109217999126256 No Hit GAGAACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 19 0.1037570991699432 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 19 0.1037570991699432 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.005460899956312801 0.0 14-15 0.0 0.0 0.0 0.005460899956312801 0.0 16-17 0.0 0.0 0.0 0.005460899956312801 0.0 18-19 0.0 0.0 0.0 0.0191131498470948 0.0 20-21 0.0 0.0 0.0 0.021843599825251202 0.0 22-23 0.0 0.0 0.0 0.030034949759720403 0.0 24-25 0.0 0.0 0.0 0.043687199650502405 0.0 26-27 0.0 0.0 0.0 0.05733944954128441 0.0 28-29 0.0 0.0 0.0 0.0655307994757536 0.0 30-31 0.0 0.0 0.0 0.07372214941022281 0.0 32-33 0.0 0.0 0.0 0.12013979903888161 0.0 34-35 0.0 0.0 0.0 0.20478374836173002 0.0 36-37 0.0 0.0 0.0 0.2976190476190476 0.0 38-39 0.0 0.0 0.0 0.43141109654871124 0.0 40-41 0.0 0.0 0.0 0.5815858453473133 0.0 42-43 0.0 0.0 0.0 0.7126474442988204 0.0 44-45 0.0 0.0 0.0 0.8300567933595457 0.0 46-47 0.0 0.0 0.0 1.0048055919615553 0.0 48-49 0.0 0.0 0.0 1.1358671909130624 0.0 50-51 0.0 0.0 0.0 1.2942332896461337 0.0 52-53 0.0 0.0 0.0 1.4143730886850152 0.0 54-55 0.0 0.0 0.0 1.602774137177807 0.0 56-57 0.0 0.0 0.0 1.8648973350808213 0.0 58-59 0.0 0.0 0.0 2.0150720838794234 0.0 60-61 0.0 0.0 0.0 2.173438182612495 0.0 62-63 0.0 0.0 0.0 2.301769331585845 0.0 64-65 0.0 0.0 0.0 2.490170380078637 0.0 66-67 0.0 0.0 0.0 2.653997378768021 0.0 68-69 0.0 0.0 0.0 2.8423984272608127 0.0 70-71 0.0 0.0 0.0 3.0471821756225426 0.0 72-73 0.0 0.0 0.0 3.3174967234600263 0.0 74-75 0.0 0.0 0.0 3.6096548711227614 0.0 76-77 0.0 0.0 0.0 3.8362822193097426 0.0 78-79 0.0 0.0 0.0 4.161205766710354 0.0 80-81 0.0 0.0 0.0 4.4397116644823065 0.0 82-83 0.0 0.0 0.0 4.685452162516382 0.0 84-85 0.0 0.0 0.0 4.920270860637833 0.0 86-87 0.0 0.0 0.0 5.250655307994758 0.0 88-89 0.0 0.0 0.0 5.561926605504587 0.0 90-91 0.0 0.0 0.0 5.982415902140673 0.0 92-93 0.0 0.0 0.0 6.337374399301005 0.0 94-95 0.0 0.0 0.0 6.56127129750983 0.0 96-97 0.0 0.0 0.0 6.9053079947575355 0.0 98-99 0.0 0.0 0.0 7.366754041065967 0.0 100-101 0.0 0.0 0.0 7.945609436435124 0.0 102-103 0.0 0.0 0.0 8.478047182175622 0.0 104-105 0.0 0.0 0.0 8.95860637833115 0.0 106-107 0.0 0.0 0.0 9.395478374836173 0.0 108-109 0.0 0.0 0.0 9.766819571865444 0.0 110-111 0.0 0.0 0.0 10.162734818698121 0.0 112-113 0.0 0.0 0.0 10.613259065093928 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCATC 15 2.6589108E-4 119.0 7 GCATCTA 15 2.6589108E-4 119.0 9 GTACAAG 20 5.544909E-6 118.99999 1 GGCATCT 20 8.34783E-4 89.24999 8 CAGGGCC 20 8.34783E-4 89.24999 4 ACATAAA 20 8.34783E-4 89.24999 3 TGGGCAT 20 8.34783E-4 89.24999 6 CATGAAA 30 4.1529485E-5 79.333336 4 CACAAAG 40 1.904822E-6 74.375 9 CACAACG 25 0.0020245533 71.4 7 AACATGA 35 8.914442E-5 68.0 2 ACATGAA 55 1.6345803E-7 64.90909 3 CAATGAA 15 0.004203901 59.5 16-17 TAGTGTG 15 0.004203901 59.5 46-47 CCCATTC 15 0.004203901 59.5 68-69 TATAGTG 15 0.004203901 59.5 44-45 CACAGAA 30 0.0041703326 59.5 7 ATGAAAA 40 1.7260696E-4 59.499996 5 GGAAACA 90 2.1827873E-11 59.499996 1 ACAACAC 115 0.0 56.913044 5 >>END_MODULE