FastQCFastQC Report
Thu 26 May 2016
SRR936206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18312
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8594.690913062472696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6863.746177370030581No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5152.812363477501092No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.9993446920052425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.8901266928789864No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.5843162953254697No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.5242463958060288No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.4477937964176496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC820.4477937964176496No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT790.43141109654871124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG750.40956749672346004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC680.37134119702927043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC500.27304499781564No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG490.2675840978593272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG480.2621231979030144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.25666229794670165No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG430.2348186981214504No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA420.22935779816513763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC420.22935779816513763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC410.2238968982088248No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.2238968982088248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.218435998252512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC390.21297509829619923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC370.2020532983835736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC360.19659239842726078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC330.18020969855832242No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG330.18020969855832242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC310.1692878986456968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.1692878986456968No Hit
GGTATCAACGCAGAGTACATGGGCTAAGTACCCCGAGGAAAAGAAATCAA300.163826998689384No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC290.15836609873307123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC290.15836609873307123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG270.1474442988204456No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT270.1474442988204456No Hit
GTACATGGGTACACCATCTAAGGCCAGGCACAGTGACTCCCGCCTGTAAT260.1419833988641328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC240.1310615989515072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA240.1310615989515072No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC230.1256006989951944No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC230.1256006989951944No Hit
ATCTAAGGCCAGGCACAGTGACTCCCGCCTGTAATCCCAGCACTTTGGGA220.1201397990388816No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT220.1201397990388816No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG210.11467889908256881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.109217999126256No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG200.109217999126256No Hit
GGCTGGAGTGCAGTGGTGCAATCTCGGCTCACCGCAACCTCCGCCTCCCA190.1037570991699432No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC190.1037570991699432No Hit
GTACATGGGCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCA190.1037570991699432No Hit
ACTTAGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTC190.1037570991699432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT190.1037570991699432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.053.5045051
GTATCAA5450.039.2693632
TATCAAC6950.031.649353
AACGCAG7150.030.7640537
TCAACGC7200.030.5504115
ATCAACG7200.030.5504114
CAACGCA7200.030.5504116
ACGCAGA7600.028.9424978
CGCAGAG7600.028.9424979
GTGGTAT2056.991104E-826.099761
TGGTATC2108.830284E-825.4783362
GCAGAGT7900.024.8333158
AGTACTT6550.024.5058812-13
CAGAGTA8100.024.2201489
GAGTACT6600.023.86985612-13
ACATGGG2602.6773705E-822.8651753
GTACATG2602.6773705E-822.8651751
TACATGG2602.6773705E-822.8651752
AGAGTAC8050.022.15507510-11
GTACTTT7400.021.28933114-15
Produced by FastQC (version 0.11.5)