##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936206_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18312 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24874399301005 31.0 31.0 34.0 30.0 34.0 2 31.006498470948014 31.0 31.0 34.0 28.0 34.0 3 31.233180428134556 31.0 31.0 34.0 28.0 34.0 4 35.24049803407602 35.0 35.0 37.0 33.0 37.0 5 34.93419615552643 35.0 35.0 37.0 32.0 37.0 6 35.28664263870686 37.0 35.0 37.0 33.0 37.0 7 35.06394713848842 37.0 35.0 37.0 32.0 37.0 8 35.2181629532547 37.0 35.0 37.0 32.0 37.0 9 36.708879423328966 39.0 37.0 39.0 32.0 39.0 10-11 36.65601791175186 39.0 35.0 39.0 32.0 39.0 12-13 36.52714067278288 39.0 35.0 39.0 32.0 39.0 14-15 37.14536915683705 39.0 36.0 41.0 31.5 41.0 16-17 37.16729467016164 39.0 36.0 40.5 31.5 41.0 18-19 37.77552970729576 39.5 36.5 41.0 32.0 41.0 20-21 37.934141546526874 40.0 37.0 41.0 32.0 41.0 22-23 37.71199213630406 40.0 36.5 41.0 32.0 41.0 24-25 37.448476408912185 40.0 36.0 41.0 32.0 41.0 26-27 36.927615771079076 39.0 35.0 41.0 31.5 41.0 28-29 36.42767038007864 39.0 35.0 41.0 31.0 41.0 30-31 35.42406618610747 37.5 35.0 40.0 29.5 41.0 32-33 34.25404106596767 35.5 33.5 39.5 28.0 41.0 34-35 33.3825633464395 35.0 33.0 39.0 23.5 41.0 36-37 32.469200524246396 35.0 33.0 39.0 16.0 41.0 38-39 31.806301878549583 35.0 33.0 39.0 3.5 41.0 40-41 31.113777850589777 35.0 32.5 39.0 2.0 41.0 42-43 30.227391874180864 35.0 31.0 39.0 2.0 41.0 44-45 28.84163390126693 35.0 25.0 39.0 2.0 41.0 46-47 26.85949104412407 35.0 7.0 39.0 2.0 41.0 48-49 25.077107907383137 34.5 2.0 39.0 2.0 41.0 50-51 24.12338903451289 33.5 2.0 39.0 2.0 41.0 52-53 23.627621231979028 33.0 2.0 39.0 2.0 41.0 54-55 23.245877020532983 33.0 2.0 38.5 2.0 41.0 56-57 22.888133464394933 33.0 2.0 38.0 2.0 41.0 58-59 22.585435779816514 33.0 2.0 38.0 2.0 41.0 60-61 22.30444517256444 32.5 2.0 37.5 2.0 40.5 62-63 21.98885976408912 31.5 2.0 37.0 2.0 40.0 64-65 21.71264744429882 31.0 2.0 36.5 2.0 40.0 66-67 21.42944517256444 31.0 2.0 36.0 2.0 40.0 68-69 21.12936871996505 30.5 2.0 36.0 2.0 39.5 70-71 20.78434906072521 30.0 2.0 35.0 2.0 39.0 72-73 20.54207623416339 29.5 2.0 35.0 2.0 39.0 74-75 20.31304608999563 29.5 2.0 35.0 2.0 38.5 76-77 20.00158366098733 29.0 2.0 35.0 2.0 37.0 78-79 19.69833988641328 28.5 2.0 35.0 2.0 37.0 80-81 19.506307339449542 28.0 2.0 35.0 2.0 36.0 82-83 19.294915902140673 27.0 2.0 35.0 2.0 36.0 84-85 19.069653778942772 26.5 2.0 35.0 2.0 36.0 86-87 18.898290738313676 26.0 2.0 35.0 2.0 35.0 88-89 18.76709261686326 26.0 2.0 35.0 2.0 35.0 90-91 18.640454346876368 26.0 2.0 35.0 2.0 35.0 92-93 18.430837702053296 25.0 2.0 35.0 2.0 35.0 94-95 18.235474006116206 24.0 2.0 35.0 2.0 35.0 96-97 18.141628440366972 23.5 2.0 35.0 2.0 35.0 98-99 17.990525338575797 23.0 2.0 35.0 2.0 35.0 100-101 17.815148536478812 19.5 2.0 35.0 2.0 35.0 102-103 17.12936871996505 17.5 2.0 33.5 2.0 35.0 104-105 17.490880297072955 17.5 2.0 34.0 2.0 35.0 106-107 17.47498907820009 14.5 2.0 34.0 2.0 35.0 108-109 17.349552206203583 8.5 2.0 34.0 2.0 35.0 110-111 17.253467671472258 2.0 2.0 34.0 2.0 35.0 112-113 17.107443206640454 2.0 2.0 34.0 2.0 35.0 114-115 17.043195718654435 2.0 2.0 34.0 2.0 35.0 116-117 16.862576452599388 2.0 2.0 34.0 2.0 35.0 118-119 16.718354084753166 2.0 2.0 34.0 2.0 35.0 120-121 16.475972040192225 2.0 2.0 34.0 2.0 35.0 122-123 16.198394495412842 2.0 2.0 34.0 2.0 35.0 124-125 14.987658366098733 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.38023219167885003 1101 2 -0.13661214066595306 1101 3 -0.3292276609537055 1101 4 0.24415819086981116 1101 5 0.3422048652380525 1101 6 0.42543793196544044 1101 7 -0.058401564583832055 1101 8 0.5253934416676884 1101 9 -0.007894476100098302 1101 10-11 0.23403385503392826 1101 12-13 0.10678503428760422 1101 14-15 -0.30348558711060747 1101 16-17 0.8704855307227177 1101 18-19 -0.21609833491484665 1101 20-21 0.26935722774088333 1101 22-23 -0.11462450720146933 1101 24-25 -0.027150202119422318 1101 26-27 0.05617134961672576 1101 28-29 -0.26664980385969983 1101 30-31 -0.10574263111008975 1101 32-33 0.41854448359922003 1101 34-35 1.2933641338440793 1101 36-37 -0.4557777049141727 1101 38-39 -0.608898291315267 1101 40-41 -2.120722024773592 1101 42-43 -2.65766659819786 1101 44-45 -2.4429283075925383 1101 46-47 -2.8975003744014245 1101 48-49 -1.6061579827496715 1101 50-51 -1.5716569104088762 1101 52-53 -0.4340903541562682 1101 54-55 -0.042897921943566786 1101 56-57 -2.1911493330939322 1101 58-59 -1.1640911466006791 1101 60-61 -2.307162844297771 1101 62-63 -1.9523231769861127 1101 64-65 -1.8499178674780232 1101 66-67 -1.520870598115593 1101 68-69 -1.5157178284649362 1101 70-71 -1.9710844825862992 1101 72-73 -0.8698807013967276 1101 74-75 -0.9609272636142094 1101 76-77 -0.321016907579871 1101 78-79 -0.6280342725721866 1101 80-81 -0.3182186796029214 1101 82-83 0.08756238300102837 1101 84-85 -0.02801027990170013 1101 86-87 -0.6819759921254196 1101 88-89 -1.3206562517212 1101 90-91 -1.1910410639321718 1101 92-93 -1.0510841035426637 1101 94-95 -0.09830198002698864 1101 96-97 -1.2743358977663277 1101 98-99 -0.96534224037336 1101 100-101 -0.6001098381143528 1101 102-103 -0.06980067866856388 1101 104-105 -0.4852239694574223 1101 106-107 -0.44078725534496144 1101 108-109 -0.3421188281960319 1101 110-111 0.4814274783918151 1101 112-113 0.9500132698445327 1101 114-115 -0.25799695590372096 1101 116-117 -0.6961646174187202 1101 118-119 -0.37470154418232227 1101 120-121 0.246634192363814 1101 122-123 0.7924572972388866 1101 124-125 0.3792608814513372 1102 1 1.0386694686021194 1102 2 1.7407824953493716 1102 3 1.7103636544996768 1102 4 1.3118465689030145 1102 5 0.6001869852891346 1102 6 1.1380816101263633 1102 7 1.2596060982514174 1102 8 0.7527242207226053 1102 9 1.1926163795831712 1102 10-11 0.9817988614196267 1102 12-13 1.2012933356924549 1102 14-15 1.257817094881723 1102 16-17 1.5033080083727839 1102 18-19 1.0080395961196373 1102 20-21 0.7406215955569735 1102 22-23 0.34961559496967 1102 24-25 0.12355222444507064 1102 26-27 0.6564267774583712 1102 28-29 1.3527627121045427 1102 30-31 1.3470032181874814 1102 32-33 1.2486849689121158 1102 34-35 2.748025691953913 1102 36-37 2.4177855134766375 1102 38-39 2.908343087995082 1102 40-41 3.7343226750986958 1102 42-43 3.526880017383238 1102 44-45 4.709689827784217 1102 46-47 4.438387237348255 1102 48-49 5.609678543431681 1102 50-51 6.454524443358682 1102 52-53 6.01546264712087 1102 54-55 6.043308974608156 1102 56-57 5.132605456178098 1102 58-59 5.420200041138784 1102 60-61 4.812249671666471 1102 62-63 5.6460165420432595 1102 64-65 5.655829258958761 1102 66-67 6.032769248627702 1102 68-69 5.603694687499303 1102 70-71 5.574253959303871 1102 72-73 5.546466680467898 1102 74-75 4.447757283001373 1102 76-77 4.74922574886968 1102 78-79 4.165069175703678 1102 80-81 4.351640835084179 1102 82-83 4.382581540089149 1102 84-85 4.438145531081695 1102 86-87 4.443822219879685 1102 88-89 4.50756852477944 1102 90-91 3.8217303281495667 1102 92-93 4.328864810889009 1102 94-95 5.02111053593725 1102 96-97 5.0168518416972745 1102 98-99 4.092767593598545 1102 100-101 3.337948910289221 1102 102-103 3.554720394128374 1102 104-105 3.659092761066205 1102 106-107 4.357424749763599 1102 108-109 4.074228553668593 1102 110-111 3.6449012970380466 1102 112-113 3.4761946236120913 1102 114-115 3.1519647299200315 1102 116-117 2.861945216553181 1102 118-119 3.1016713804664633 1102 120-121 3.265152710882333 1102 122-123 3.602163555221008 1102 124-125 3.243884125384927 1103 1 0.08093865658877064 1103 2 0.2469004041369125 1103 3 0.06742705828165896 1103 4 -0.04855387603580397 1103 5 0.7281068963013766 1103 6 -0.1444545411528395 1103 7 0.7623869658821221 1103 8 -0.2428263910682702 1103 9 0.20596454421053068 1103 10-11 0.1330780605297477 1103 12-13 0.18683282401280366 1103 14-15 1.311209753391175 1103 16-17 0.15125016632606503 1103 18-19 0.23551456831096118 1103 20-21 0.054303464300019755 1103 22-23 -1.6170139934619243 1103 24-25 -0.3742230814503671 1103 26-27 -1.6272217208731163 1103 28-29 -0.6561360643137064 1103 30-31 -0.08125039694044034 1103 32-33 -0.592805576137934 1103 34-35 -0.8559787574342934 1103 36-37 -1.3697561995378251 1103 38-39 -2.431478936476555 1103 40-41 -1.585477102431895 1103 42-43 -1.0220632529170963 1103 44-45 -1.608997602694096 1103 46-47 -1.7368074233858941 1103 48-49 -1.9281412563458495 1103 50-51 -1.4097692162631184 1103 52-53 -1.383313771121621 1103 54-55 -2.317091470330663 1103 56-57 -1.1983177918752972 1103 58-59 -0.3773527953462015 1103 60-61 -1.3579394273324183 1103 62-63 -1.300590799447285 1103 64-65 -0.6940038889833993 1103 66-67 -1.0656734654991027 1103 68-69 -0.7761718308544232 1103 70-71 -0.6281932042111471 1103 72-73 -1.4134888256500098 1103 74-75 -1.405969079623766 1103 76-77 -0.8992725826097363 1103 78-79 -0.740937498378635 1103 80-81 -1.0488041037367353 1103 82-83 -1.0701437101889368 1103 84-85 -0.9139123109650207 1103 86-87 -0.4304825632126352 1103 88-89 -1.1259131214941966 1103 90-91 -0.8445655561663443 1103 92-93 -0.34021194105759633 1103 94-95 -0.3103688856422835 1103 96-97 -0.7617910948989461 1103 98-99 0.22402454576761954 1103 100-101 -0.28051605077862973 1103 102-103 -0.4335999618226829 1103 104-105 0.5267234618448491 1103 106-107 0.455867463890403 1103 108-109 0.7271762733571343 1103 110-111 0.9969591390847654 1103 112-113 0.9667396736677105 1103 114-115 1.21870555305685 1103 116-117 1.2190086203351633 1103 118-119 1.1617381212895985 1103 120-121 0.8135398076565252 1103 122-123 1.2267464250764029 1103 124-125 1.6904914668754714 1104 1 -0.17282478427144454 1104 2 0.5189434148896019 1104 3 0.0781797464536993 1104 4 -0.0410269943153736 1104 5 0.3829456059787901 1104 6 0.2254379319654447 1104 7 -0.41395712013938635 1104 8 -0.6449769287026825 1104 9 -0.23382040202602639 1104 10-11 0.37662644762652064 1104 12-13 0.41048873799130803 1104 14-15 -0.014596698221723159 1104 16-17 -0.18692187668469273 1104 18-19 0.20056833175182476 1104 20-21 -0.0806427722591252 1104 22-23 -0.4164763590533198 1104 24-25 -0.7752983502675761 1104 26-27 -1.1567916133462361 1104 28-29 -2.1851683223782246 1104 30-31 -2.374261149628616 1104 32-33 -2.8277518126970733 1104 34-35 -3.764043273563324 1104 36-37 -3.3057777049141706 1104 38-39 -3.4588982913152684 1104 40-41 -4.272573876625444 1104 42-43 -4.848407338938603 1104 44-45 -3.6466320112962407 1104 46-47 -7.04379667069772 1104 48-49 -8.326528353120043 1104 50-51 -7.908693947445915 1104 52-53 -7.7655718356377506 1104 54-55 -7.3428979219435675 1104 56-57 -8.29670488864949 1104 58-59 -8.02520225771179 1104 60-61 -8.309014696149625 1104 62-63 -8.82639725106019 1104 64-65 -8.04991786747802 1104 66-67 -7.743092820337814 1104 68-69 -7.667569680316788 1104 70-71 -7.897010408512223 1104 72-73 -7.566176997693024 1104 74-75 -7.018334671021616 1104 76-77 -6.635831722394686 1104 78-79 -6.794700939238854 1104 80-81 -6.455255716639959 1104 82-83 -6.567993172554527 1104 84-85 -6.285417687309106 1104 86-87 -5.74123525138468 1104 88-89 -5.878063659128607 1104 90-91 -6.139189212080321 1104 92-93 -6.391824844283406 1104 94-95 -5.616820498545508 1104 96-97 -5.568780342210772 1104 98-99 -5.756082981114098 1104 100-101 -4.848257986262501 1104 102-103 -5.380911789779674 1104 104-105 -5.875964710198165 1104 106-107 -6.483379847937552 1104 108-109 -7.088415124492327 1104 110-111 -7.081535484571148 1104 112-113 -6.9203571005258375 1104 114-115 -7.2135525114592784 1104 116-117 -6.870238691492794 1104 118-119 -6.867294136774918 1104 120-121 -6.451513955784334 1104 122-123 -6.283468628687039 1104 124-125 -5.500368748178291 1105 1 1.1271752157285526 1105 2 1.485610081556267 1105 3 0.8115130797870371 1105 4 0.6256396723512907 1105 5 0.8162789393121272 1105 6 0.8254379319654461 1105 7 0.7527095465272851 1105 8 0.888356404630656 1105 9 1.6661795979739722 1105 10-11 1.3266264476265235 1105 12-13 0.8104887379912995 1105 14-15 0.7187366351116111 1105 16-17 1.1130781233153044 1105 18-19 0.8005683317518191 1105 20-21 0.8360238944075462 1105 22-23 2.0668569742800145 1105 24-25 1.291368316399101 1105 26-27 1.0932083866537639 1105 28-29 0.8648316776217868 1105 30-31 0.2090721837047198 1105 32-33 0.3555815206362638 1105 34-35 -0.080709940229994 1105 36-37 0.527555628419158 1105 38-39 0.9244350420180645 1105 40-41 1.360759456707882 1105 42-43 0.6849259943947317 1105 44-45 -0.4799653446295764 1105 46-47 -1.3271300040310514 1105 48-49 -1.7598616864533732 1105 50-51 -1.6753606141125807 1105 52-53 -2.5155718356377506 1105 54-55 -2.792897921943567 1105 56-57 -2.1967048886494887 1105 58-59 -1.891868924378457 1105 60-61 -1.3090146961496245 1105 62-63 -2.8430639177268553 1105 64-65 -2.3499178674780232 1105 66-67 -2.1930928203378137 1105 68-69 -1.667569680316788 1105 70-71 -1.930343741845558 1105 72-73 -1.8495103310263552 1105 74-75 -1.3683346710216178 1105 76-77 -1.9858317223946855 1105 78-79 -2.111367605905521 1105 80-81 -2.421922383306626 1105 82-83 -2.901326505887859 1105 84-85 -1.685417687309105 1105 86-87 -2.157901918051344 1105 88-89 -3.094730325795272 1105 90-91 -2.722522545413657 1105 92-93 -2.291824844283404 1105 94-95 -2.566820498545507 1105 96-97 -2.2521136755441056 1105 98-99 -2.606082981114099 1105 100-101 -2.1482579862625 1105 102-103 -2.0642451231130075 1105 104-105 -2.3926313768648306 1105 106-107 -2.3167131812708845 1105 108-109 -1.888415124492326 1105 110-111 -2.1482021512378147 1105 112-113 -2.120357100525837 1105 114-115 -2.7635525114592774 1105 116-117 -4.303572024826128 1105 118-119 -5.150627470108251 1105 120-121 -5.518180622451 1105 122-123 -4.883468628687041 1105 124-125 -4.367035414844958 1106 1 0.3712612372339308 1106 2 1.0210939525240086 1106 3 0.5190399615074632 1106 4 0.435317091706132 1106 5 0.7926230253336328 1106 6 0.7910293298149043 1106 7 0.8914192239466345 1106 8 1.1120123186091462 1106 9 1.0769322861460182 1106 10-11 1.1492070927878189 1106 12-13 0.7190908885289389 1106 14-15 0.6499194308105345 1106 16-17 0.8447985534228337 1106 18-19 1.203256503794833 1106 20-21 1.3123679804290447 1106 22-23 0.5281472968606522 1106 24-25 0.9806156282270564 1106 26-27 0.19535892428817903 1106 28-29 -0.30129735463628293 1106 30-31 -0.6296374937146396 1106 32-33 0.4717105528943222 1106 34-35 0.27305350063022615 1106 36-37 0.32379218755894357 1106 38-39 1.3265855796524768 1106 40-41 2.6080712846648737 1106 42-43 1.3650335212764482 1106 44-45 1.3426153005317119 1106 46-47 1.27932160887217 1106 48-49 1.3137942275251184 1106 50-51 1.7353920740594617 1106 52-53 0.8102346159751548 1106 54-55 0.10226336837901329 1106 56-57 0.25329511135051064 1106 58-59 0.21942139820218998 1106 60-61 0.36786702428048557 1106 62-63 0.3929575876494873 1106 64-65 0.03180256262950465 1106 66-67 0.7085200828879934 1106 68-69 1.030279782048801 1106 70-71 0.8234196990146572 1106 72-73 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124-125 -2.0096280074375503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 0.0 4 5.0 5 24.0 6 63.0 7 151.0 8 212.0 9 318.0 10 522.0 11 738.0 12 829.0 13 1102.0 14 1463.0 15 1214.0 16 427.0 17 269.0 18 224.0 19 193.0 20 200.0 21 172.0 22 174.0 23 186.0 24 182.0 25 198.0 26 227.0 27 253.0 28 272.0 29 324.0 30 405.0 31 423.0 32 483.0 33 565.0 34 819.0 35 1189.0 36 1619.0 37 2040.0 38 817.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.83606557377048 16.42622950819672 16.825136612021858 12.912568306010929 2 21.597859327217126 26.79663608562691 32.366754041065974 19.238750546089996 3 19.588248143294017 28.72433377020533 38.29183049366536 13.395587592835298 4 10.943643512450851 21.794451725644386 30.96876365224989 36.29314110965487 5 7.1249043820347495 42.62922085018031 34.53720904819145 15.708665719593487 6 22.89755351681957 40.20860637833115 13.772389689820882 23.121450415028395 7 14.455002184359984 42.15268676277851 22.63543031891656 20.756880733944953 8 29.97487986020096 31.53123634775011 18.228484054172128 20.265399737876802 9 21.242900830056794 10.572302315421583 21.45041502839668 46.734381826124945 10-11 24.557667103538662 27.33453473132372 23.888706858890345 24.21909130624727 12-13 29.554390563564876 29.87931411096549 27.845128877238967 12.721166448230669 14-15 23.113259065093928 27.118829183049364 36.75731760594146 13.010594145915247 16-17 12.764853647881171 26.27238968982088 39.47957623416339 21.483180428134556 18-19 11.869266055045872 16.737658366098735 54.32503276539974 17.06804281345566 20-21 13.188073394495412 14.941022280471822 59.03232852774137 12.838575797291393 22-23 12.538226299694188 15.45161642638707 59.46100917431193 12.549148099606816 24-25 12.527304499781563 15.156727828746178 60.023481869812144 12.292485801660114 26-27 12.524574049803409 14.605176933158583 60.05351681957186 12.816732197466143 28-29 12.21876365224989 14.872761031017912 60.44124071647008 12.467234600262122 30-31 12.625600698995196 15.164919178680647 59.570227173438184 12.639252948885977 32-33 11.88564875491481 15.585408475316731 59.08966797728266 13.439274792485802 34-35 11.478811708169506 16.183377020532987 58.83846657929227 13.499344692005241 36-37 13.188073394495412 15.809305373525556 57.131935342944516 13.870685889034512 38-39 12.964176496286589 15.134884228920924 56.35102664919178 15.549912625600697 40-41 13.420161642638707 15.443425076452598 55.930537352555696 15.205875928352993 42-43 14.886413280908695 16.62024901703801 53.251965923984265 15.241371778069027 44-45 14.78032930125877 17.027550992545667 50.85328891680092 17.338830789394642 46-47 15.562109172332814 17.268780208077334 47.672100707244475 19.497009912345376 48-49 17.175468297744526 18.6090328218011 42.76118180328764 21.454317077166728 50-51 17.39213544511196 19.43200436919716 41.048607318405246 22.127252867285634 52-53 17.5058707880509 20.345693845229643 39.899513953361364 22.24892141335809 54-55 16.950633464394933 21.076343381389254 39.13007863695937 22.842944517256445 56-57 16.2002020588155 20.63730442618027 38.707915790623375 24.454577724380854 58-59 16.683049366535606 19.722040192223677 37.96144604630843 25.633464394932286 60-61 16.48144608579308 19.75534500177484 38.73522103596101 25.02798787647107 62-63 16.551987767584098 19.307011795543904 38.769659239842724 25.371341197029267 64-65 15.65366972477064 19.48995194408038 39.684359982525116 25.172018348623855 66-67 16.762690112770663 19.348496846244164 39.1775660104306 24.71124703055457 68-69 17.73154215814766 19.039427697684577 38.01878549584971 25.210244648318042 70-71 17.338830789394642 19.556016710810148 39.103841848019 24.001310651776205 72-73 18.385123696138933 19.630276882748074 39.14586860357163 22.838730817541368 74-75 17.36020096111839 20.186216688510267 38.36555264307558 24.088029707295764 76-77 17.160878112712975 20.45653123634775 38.67955439056357 23.70303626037571 78-79 17.401157710790738 20.418304936653563 38.38739624290083 23.793141109654872 80-81 16.833224115334207 20.16983398864133 38.941677588466575 24.055264307557884 82-83 16.871450415028395 21.439493228484054 37.15050240279598 24.53855395369157 84-85 16.628440366972477 21.565093927479246 36.779161205766705 25.027304499781565 86-87 15.945827872433377 22.64089121887287 35.594145915246834 25.81913499344692 88-89 15.899410222804718 22.531673219746615 34.06236347750109 27.506553079947576 90-91 16.726736566186105 22.651813018785496 32.183813892529486 28.43763652249891 92-93 16.161533420707734 22.908475316732197 31.62953254696374 29.300458715596328 94-95 15.776539973787681 23.68665356050677 31.85069899519441 28.68610747051114 96-97 16.745849716033202 24.085299257317605 29.000109217999125 30.16874180865007 98-99 17.859873307121013 23.97608125819135 27.53112712975098 30.632918304936652 100-101 17.401157710790738 24.655963302752294 27.088794233289647 30.85408475316732 102-103 17.742463958060288 25.66622979467016 26.550895587592837 30.040410659676713 104-105 17.592289209261686 26.1085626911315 27.154325032765396 29.144823066841415 106-107 17.843490607252075 26.96592398427261 27.33453473132372 27.856050677151593 108-109 18.067880839909346 27.979684897468804 26.824673019687083 27.127761242934767 110-111 17.61959370904325 28.276539973787678 27.607579729139363 26.496286588029704 112-113 18.226748948719347 28.649445688384027 27.213150565234013 25.91065479766261 114-115 17.91468676607133 27.677644874105635 27.707684745207274 26.699983614615764 116-117 17.81637446064777 27.087771041564256 28.87923971817139 26.21661477961658 118-119 17.84222167608749 26.66502826247235 29.362933835777287 26.12981622566287 120-121 18.041068210365356 27.052045218720988 29.973786248702964 24.933100322210695 122-123 17.508123310487946 26.720913087404092 30.101302460202607 25.669661141905358 124-125 17.428462210572302 26.1085626911315 30.02675840978593 26.436216688510267 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 12.0 2 25.5 3 51.0 4 40.0 5 39.5 6 64.5 7 166.5 8 231.5 9 230.0 10 211.5 11 130.5 12 93.0 13 83.5 14 81.0 15 85.0 16 88.5 17 93.0 18 95.0 19 96.0 20 112.0 21 122.0 22 137.0 23 154.5 24 168.0 25 205.0 26 261.0 27 292.5 28 341.0 29 412.5 30 497.0 31 573.0 32 595.0 33 643.5 34 688.5 35 667.0 36 635.0 37 600.5 38 576.5 39 508.5 40 432.5 41 406.0 42 365.5 43 343.0 44 324.0 45 334.5 46 331.5 47 317.0 48 362.0 49 417.5 50 543.0 51 543.0 52 337.5 53 261.5 54 284.0 55 288.5 56 322.5 57 335.5 58 307.5 59 229.5 60 158.5 61 136.5 62 117.5 63 105.0 64 83.0 65 65.5 66 62.5 67 50.5 68 47.0 69 51.5 70 42.0 71 34.0 72 32.0 73 31.5 74 29.5 75 22.5 76 15.0 77 9.0 78 7.5 79 6.5 80 2.5 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0655307994757536 2 0.0 3 0.0 4 0.0 5 0.054608999563128 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0027304499781564003 46-47 0.0081913499344692 48-49 0.005460899956312801 50-51 0.010921799912625601 52-53 0.005460899956312801 54-55 0.0 56-57 0.0027304499781564003 58-59 0.0 60-61 0.0027304499781564003 62-63 0.0 64-65 0.0 66-67 0.0027304499781564003 68-69 0.0 70-71 0.0027304499781564003 72-73 0.005460899956312801 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0027304499781564003 110-111 0.0 112-113 0.005460899956312801 114-115 0.0163826998689384 116-117 0.0163826998689384 118-119 0.0081913499344692 120-121 0.005460899956312801 122-123 0.0027304499781564003 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 18312.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.5869375273045 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9691712577658 33.99410222804718 2 10.971750087914664 10.222804718217562 3 6.493963193060602 9.076015727391875 4 3.7979134919704607 7.077326343381389 5 1.9224006564294924 4.477937964176496 6 0.9963661938811393 2.7850589777195283 7 0.7150392685499942 2.331804281345566 8 0.5509318954401594 2.053298383573613 9 0.3399367014418005 1.4252948885976409 >10 1.1018637908803188 10.20096111839231 >50 0.07033173133278631 2.63215377894277 >100 0.035165865666393153 2.4737876802096985 >500 0.035165865666393153 11.249453910004368 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 859 4.690913062472696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 686 3.746177370030581 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 515 2.812363477501092 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 183 0.9993446920052425 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 0.8901266928789864 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.5843162953254697 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.5242463958060288 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.4477937964176496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 82 0.4477937964176496 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 79 0.43141109654871124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 75 0.40956749672346004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 68 0.37134119702927043 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 50 0.27304499781564 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 49 0.2675840978593272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 48 0.2621231979030144 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.25666229794670165 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 43 0.2348186981214504 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 42 0.22935779816513763 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 42 0.22935779816513763 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 41 0.2238968982088248 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.2238968982088248 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 40 0.218435998252512 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 39 0.21297509829619923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 37 0.2020532983835736 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 36 0.19659239842726078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 33 0.18020969855832242 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 33 0.18020969855832242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 31 0.1692878986456968 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 31 0.1692878986456968 No Hit GGTATCAACGCAGAGTACATGGGCTAAGTACCCCGAGGAAAAGAAATCAA 30 0.163826998689384 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 29 0.15836609873307123 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 29 0.15836609873307123 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 27 0.1474442988204456 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 27 0.1474442988204456 No Hit GTACATGGGTACACCATCTAAGGCCAGGCACAGTGACTCCCGCCTGTAAT 26 0.1419833988641328 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 24 0.1310615989515072 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 24 0.1310615989515072 No Hit CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC 23 0.1256006989951944 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 23 0.1256006989951944 No Hit ATCTAAGGCCAGGCACAGTGACTCCCGCCTGTAATCCCAGCACTTTGGGA 22 0.1201397990388816 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 22 0.1201397990388816 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 21 0.11467889908256881 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 20 0.109217999126256 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 20 0.109217999126256 No Hit GGCTGGAGTGCAGTGGTGCAATCTCGGCTCACCGCAACCTCCGCCTCCCA 19 0.1037570991699432 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 19 0.1037570991699432 No Hit GTACATGGGCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCA 19 0.1037570991699432 No Hit ACTTAGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTC 19 0.1037570991699432 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 19 0.1037570991699432 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.005460899956312801 0.0 14-15 0.0 0.0 0.0 0.005460899956312801 0.0 16-17 0.0 0.0 0.0 0.010921799912625601 0.0 18-19 0.0 0.0 0.0 0.0245740498034076 0.0 20-21 0.0 0.0 0.0 0.027304499781564 0.0 22-23 0.0 0.0 0.0 0.0354958497160332 0.0 24-25 0.0 0.0 0.0 0.0491480996068152 0.0 26-27 0.0 0.0 0.0 0.0628003494975972 0.0 28-29 0.0 0.0 0.0 0.0709916994320664 0.0 30-31 0.0 0.0 0.0 0.08464394932284841 0.0 32-33 0.0 0.0 0.0 0.1310615989515072 0.0 34-35 0.0 0.0 0.0 0.21570554827435562 0.0 36-37 0.0 0.0 0.0 0.31673219746614245 0.0 38-39 0.0 0.0 0.0 0.45598514635211884 0.0 40-41 0.0 0.0 0.0 0.6034294451725644 0.0 42-43 0.0 0.0 0.0 0.7344910441240717 0.0 44-45 0.0 0.0 0.0 0.8628221930974225 0.0 46-47 0.0 0.0 0.0 1.0403014416775884 0.0 48-49 0.0 0.0 0.0 1.1713630406290956 0.0 50-51 0.0 0.0 0.0 1.3406509392747925 0.0 52-53 0.0 0.0 0.0 1.4717125382262997 0.0 54-55 0.0 0.0 0.0 1.6737658366098733 0.0 56-57 0.0 0.0 0.0 1.9495412844036697 0.0 58-59 0.0 0.0 0.0 2.1133682830930534 0.0 60-61 0.0 0.0 0.0 2.301769331585845 0.0 62-63 0.0 0.0 0.0 2.4382918304936654 0.0 64-65 0.0 0.0 0.0 2.637614678899083 0.0 66-67 0.0 0.0 0.0 2.839667977282656 0.0 68-69 0.0 0.0 0.0 3.0526430755788554 0.0 70-71 0.0 0.0 0.0 3.322957623416339 0.0 72-73 0.0 0.0 0.0 3.642420270860638 0.0 74-75 0.0 0.0 0.0 3.95915246832678 0.0 76-77 0.0 0.0 0.0 4.196701616426386 0.0 78-79 0.0 0.0 0.0 4.557121013543032 0.0 80-81 0.0 0.0 0.0 4.8711227610310175 0.0 82-83 0.0 0.0 0.0 5.1550895587592835 0.0 84-85 0.0 0.0 0.0 5.417212756662298 0.0 86-87 0.0 0.0 0.0 5.763979903888162 0.0 88-89 0.0 0.0 0.0 6.0834425513324595 0.0 90-91 0.0 0.0 0.0 6.514853647881171 0.0 92-93 0.0 0.0 0.0 6.8643512450851905 0.0 94-95 0.0 0.0 0.0 7.093709043250328 0.0 96-97 0.0 0.0 0.0 7.44866754041066 0.0 98-99 0.0 0.0 0.0 7.945609436435125 0.0 100-101 0.0 0.0 0.0 8.543577981651376 0.0 102-103 0.0 0.0 0.0 9.114242027086064 0.0 104-105 0.0 0.0 0.0 9.613914373088685 0.0 106-107 0.0 0.0 0.0 10.045325469637396 0.0 108-109 0.0 0.0 0.0 10.43031891655745 0.0 110-111 0.0 0.0 0.0 10.8781127129751 0.0 112-113 0.0 0.0 0.0 11.358671909130624 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 400 0.0 53.504505 1 GTATCAA 545 0.0 39.269363 2 TATCAAC 695 0.0 31.64935 3 AACGCAG 715 0.0 30.764053 7 TCAACGC 720 0.0 30.550411 5 ATCAACG 720 0.0 30.550411 4 CAACGCA 720 0.0 30.550411 6 ACGCAGA 760 0.0 28.942497 8 CGCAGAG 760 0.0 28.942497 9 GTGGTAT 205 6.991104E-8 26.09976 1 TGGTATC 210 8.830284E-8 25.478336 2 GCAGAGT 790 0.0 24.833315 8 AGTACTT 655 0.0 24.50588 12-13 CAGAGTA 810 0.0 24.220148 9 GAGTACT 660 0.0 23.869856 12-13 ACATGGG 260 2.6773705E-8 22.865175 3 GTACATG 260 2.6773705E-8 22.865175 1 TACATGG 260 2.6773705E-8 22.865175 2 AGAGTAC 805 0.0 22.155075 10-11 GTACTTT 740 0.0 21.289331 14-15 >>END_MODULE