FastQCFastQC Report
Thu 26 May 2016
SRR936205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029771
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.068.564311
GTACATG128600.053.2481081
TACATGG131050.051.6624572
ACATGGG131700.049.736063
CATGGGA92150.047.5819244
GTATCAA32700.046.7579232
CATGGGG34900.046.196994
ATGGGAG32250.043.905315
ATGGGGG20450.040.4381525
CGTATAG1053.2660682E-739.662361
ATGGGAT35900.037.949925
CAACGCA41550.037.9441346
TCAACGC41350.037.8399055
AACGCAG42100.037.5897457
ATCAACG42100.037.16584
ATGGGGA14150.033.6359255
TATCAAC47200.033.2760473
ATGGGAA34800.032.4821055
AGTACAT74000.032.2390672
GAGTACA76850.032.1272851
Produced by FastQC (version 0.11.5)