##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936205_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029771 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.08882751602055 31.0 30.0 34.0 26.0 34.0 2 30.033926960460143 31.0 30.0 34.0 26.0 34.0 3 30.17681406837054 31.0 30.0 34.0 26.0 34.0 4 33.74093754825102 37.0 35.0 37.0 28.0 37.0 5 33.93093221696863 35.0 35.0 37.0 30.0 37.0 6 34.00376200145469 37.0 35.0 37.0 30.0 37.0 7 33.84935679874457 37.0 35.0 37.0 30.0 37.0 8 33.82768887451676 37.0 35.0 37.0 29.0 37.0 9 35.26745460884022 39.0 35.0 39.0 29.0 39.0 10-11 35.10682375013474 39.0 35.0 39.0 27.5 39.0 12-13 35.12368575149232 39.0 35.0 39.0 27.5 39.0 14-15 36.240523378498715 40.0 36.0 41.0 27.5 41.0 16-17 36.25959412335364 40.0 36.0 41.0 28.0 41.0 18-19 36.32236196202845 40.0 36.0 41.0 28.0 41.0 20-21 36.46195270598997 40.0 36.0 41.0 29.0 41.0 22-23 36.34658870758644 40.0 36.0 41.0 28.5 41.0 24-25 36.31520454547662 39.0 36.0 41.0 28.5 41.0 26-27 36.430964262928356 39.5 36.0 41.0 29.0 41.0 28-29 36.33238117989339 39.0 36.0 41.0 29.0 41.0 30-31 36.25959994989177 39.0 36.0 41.0 29.0 41.0 32-33 36.10911309407626 39.0 35.0 41.0 28.5 41.0 34-35 36.07371104837871 39.0 35.0 41.0 29.0 41.0 36-37 35.9622163568405 39.0 35.0 41.0 28.0 41.0 38-39 35.72145651800254 39.0 35.0 41.0 27.0 41.0 40-41 35.82717322589197 39.0 35.0 40.5 27.5 41.0 42-43 35.7355460582984 39.0 35.0 41.0 27.0 41.0 44-45 35.60109820532915 39.0 35.0 41.0 27.0 41.0 46-47 35.591462567891305 39.0 35.0 40.5 27.0 41.0 48-49 35.50764975902409 39.0 34.5 40.0 27.0 41.0 50-51 35.37099996018532 39.0 34.0 40.0 27.0 41.0 52-53 35.232723586117686 38.5 34.0 40.0 27.0 41.0 54-55 35.058263924697826 38.0 34.0 40.0 27.0 41.0 56-57 34.875458718491785 38.0 34.0 40.0 26.0 41.0 58-59 34.63799864241662 38.0 33.0 40.0 26.0 41.0 60-61 34.39674014902342 38.0 33.0 40.0 26.0 41.0 62-63 34.1259168300525 37.0 33.0 40.0 26.0 41.0 64-65 33.84585990477495 37.0 33.0 40.0 25.0 41.0 66-67 33.53958307235298 36.0 32.0 39.0 24.5 41.0 68-69 33.231853975301306 36.0 32.0 39.0 23.5 41.0 70-71 32.88286424845912 35.5 32.0 39.0 23.0 40.0 72-73 32.548709373249004 35.0 32.0 38.5 22.5 40.0 74-75 32.1934386382992 35.0 31.0 37.0 22.0 39.5 76-77 31.854703618571506 35.0 31.0 37.0 21.5 39.0 78-79 31.516095326048216 35.0 31.0 36.5 20.5 39.0 80-81 31.19861260416151 35.0 31.0 36.0 20.0 38.0 82-83 30.903600897675307 34.0 31.0 35.5 20.0 37.0 84-85 30.940912591246015 35.0 31.0 35.0 20.0 37.0 86-87 30.82411526446171 35.0 31.0 35.0 20.0 36.5 88-89 30.64488658157979 34.5 31.0 35.0 19.5 36.0 90-91 30.47420203132541 34.0 31.0 35.0 18.5 36.0 92-93 30.343923066390488 34.0 31.0 35.0 18.0 36.0 94-95 30.221317652177035 34.0 31.0 35.0 17.5 35.0 96-97 30.12256122963261 34.0 31.0 35.0 15.5 35.0 98-99 30.01476444762962 34.0 31.0 35.0 12.0 35.0 100-101 29.91672371818589 34.0 31.0 35.0 9.0 35.0 102-103 28.91922912958318 33.5 29.0 34.5 7.0 35.0 104-105 29.594839046739516 34.0 30.0 35.0 7.0 35.0 106-107 29.70315827499512 34.0 30.5 35.0 3.5 35.0 108-109 29.646337389574963 34.0 30.5 35.0 2.0 35.0 110-111 29.52716186414261 34.0 30.0 35.0 2.0 35.0 112-113 29.47577665325592 34.0 30.0 35.0 2.0 35.0 114-115 29.34355987884685 34.0 30.0 35.0 2.0 35.0 116-117 29.197224431451264 34.0 30.0 35.0 2.0 35.0 118-119 28.97567614547312 34.0 30.0 35.0 2.0 35.0 120-121 28.742919056761163 34.0 30.0 35.0 2.0 35.0 122-123 28.417886598088312 34.0 29.0 35.0 2.0 35.0 124-125 26.33421751049505 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24765259695497122 1101 2 0.33247466477682863 1101 3 0.33209735490649095 1101 4 0.2563296106071675 1101 5 0.261275723845273 1101 6 0.1944725117099182 1101 7 0.23034892239734717 1101 8 0.2960361312392408 1101 9 0.2461873916616497 1101 10-11 0.35741252535437695 1101 12-13 0.27560098071001704 1101 14-15 0.45612865727106566 1101 16-17 0.37712918380917415 1101 18-19 0.30307813227051383 1101 20-21 0.17339178009248712 1101 22-23 0.5235278191320418 1101 24-25 0.6560734017681256 1101 26-27 0.6472031206862852 1101 28-29 0.44833512692154187 1101 30-31 0.44886203595112306 1101 32-33 0.6239742936553725 1101 34-35 0.5330617284875245 1101 36-37 0.5114336212647572 1101 38-39 0.7151744263122026 1101 40-41 0.6557650950864655 1101 42-43 0.6984310885045844 1101 44-45 0.6968784662022998 1101 46-47 0.448969165708192 1101 48-49 0.4969035267257027 1101 50-51 0.573191267062505 1101 52-53 0.550019802319845 1101 54-55 0.48142317685685754 1101 56-57 0.7031710794958315 1101 58-59 0.5991443817158171 1101 60-61 0.6891987506780666 1101 62-63 0.6795886372001334 1101 64-65 0.6547241322645263 1101 66-67 0.5872119379553595 1101 68-69 0.63775912770312 1101 70-71 0.6374373653034624 1101 72-73 0.6690109572714604 1101 74-75 0.5052150720909196 1101 76-77 0.4783745039706844 1101 78-79 0.4680137252420593 1101 80-81 0.4218820596955588 1101 82-83 0.36988341321798757 1101 84-85 0.14772463733672225 1101 86-87 0.3013477117815029 1101 88-89 0.21110435133168437 1101 90-91 0.20575213190008768 1101 92-93 0.09485381390316405 1101 94-95 0.14632071955770343 1101 96-97 0.06221071030898173 1101 98-99 0.1558919553646696 1101 100-101 0.1375293134775184 1101 102-103 0.26591234106435024 1101 104-105 0.09822430736917198 1101 106-107 -0.024151043615390222 1101 108-109 -0.055568885569975635 1101 110-111 0.11205719949760251 1101 112-113 0.028526360612964652 1101 114-115 0.15319060528206663 1101 116-117 0.0073263939463004135 1101 118-119 0.01048232333178234 1101 120-121 -0.13015150645623308 1101 122-123 -0.03863956659338186 1101 124-125 -0.15519392858978875 1102 1 0.43299339450931384 1102 2 0.3391001308057007 1102 3 0.3703832997472922 1102 4 0.4020417570362511 1102 5 0.29975991280955583 1102 6 0.3836377526482977 1102 7 0.44512373491892987 1102 8 0.45412553895097574 1102 9 0.5671620854695263 1102 10-11 0.7132973885814522 1102 12-13 0.590420213881572 1102 14-15 0.8174586386880947 1102 16-17 0.7546936519446277 1102 18-19 0.7849527612901923 1102 20-21 0.7259445126413766 1102 22-23 0.7969627186327415 1102 24-25 0.8684328172001301 1102 26-27 0.6315969242965735 1102 28-29 0.705017816058465 1102 30-31 0.7211586805432404 1102 32-33 0.8616965423877332 1102 34-35 0.671242344014189 1102 36-37 0.5528705226043584 1102 38-39 0.737637696835904 1102 40-41 0.6704213878825627 1102 42-43 0.7567067137882759 1102 44-45 0.7122734220338174 1102 46-47 0.5102865386437969 1102 48-49 0.5756774391956796 1102 50-51 0.5750959236656641 1102 52-53 0.5890682693224818 1102 54-55 0.6728717167947025 1102 56-57 0.7747418635105987 1102 58-59 0.7375533077447685 1102 60-61 0.7947036136723611 1102 62-63 0.6367642209728217 1102 64-65 0.4427243495183575 1102 66-67 0.4934374241578041 1102 68-69 0.5431982326173213 1102 70-71 0.5508834378584808 1102 72-73 0.4951603658366892 1102 74-75 0.48361790725345344 1102 76-77 0.3611181483261561 1102 78-79 0.3811678629577173 1102 80-81 0.4806575627739669 1102 82-83 0.5129291567886298 1102 84-85 0.4758206014588069 1102 86-87 0.5540769630704787 1102 88-89 0.4786305284789094 1102 90-91 0.5835961203701139 1102 92-93 0.616071366461135 1102 94-95 0.5206337512961632 1102 96-97 0.6243557815565985 1102 98-99 0.5440070067878757 1102 100-101 0.5746030983108739 1102 102-103 0.6671004479687532 1102 104-105 0.6668877695417876 1102 106-107 0.6812048453213464 1102 108-109 0.7591256206236956 1102 110-111 0.8507551507163598 1102 112-113 0.7548590771005941 1102 114-115 0.8835727780531784 1102 116-117 0.8042119050553467 1102 118-119 0.9301655206874955 1102 120-121 0.8479828650400627 1102 122-123 0.9019775894763384 1102 124-125 0.8378399824059706 1103 1 0.24443425354284187 1103 2 0.2499338914581557 1103 3 0.21638359276612107 1103 4 0.4244485404220839 1103 5 0.3009518279317689 1103 6 0.4399278901229664 1103 7 0.2879870477409483 1103 8 0.38370334765851766 1103 9 0.6414721177015963 1103 10-11 0.5788460052884119 1103 12-13 0.48895366991653333 1103 14-15 0.5391498914656694 1103 16-17 0.4487900093543473 1103 18-19 0.45782547513289273 1103 20-21 0.26027388106371063 1103 22-23 0.39435141726288236 1103 24-25 0.5484318404707125 1103 26-27 0.3213968168563355 1103 28-29 0.45769466512935253 1103 30-31 0.3985109960024289 1103 32-33 0.600758699006299 1103 34-35 0.5739856564699295 1103 36-37 0.5443912903136834 1103 38-39 0.49247365207727967 1103 40-41 0.546712502086649 1103 42-43 0.7271980392101014 1103 44-45 0.622770668877763 1103 46-47 0.6648661787189951 1103 48-49 0.4744123456738407 1103 50-51 0.38513349722559553 1103 52-53 0.3556078085503742 1103 54-55 0.362913120965338 1103 56-57 0.4874555993785492 1103 58-59 0.38449232930607025 1103 60-61 0.3717366607018846 1103 62-63 0.44830656829780935 1103 64-65 0.35617353211792135 1103 66-67 0.28099228888078187 1103 68-69 0.30329250226790094 1103 70-71 0.3098323396483309 1103 72-73 0.24804934832881287 1103 74-75 0.2656340293934676 1103 76-77 0.17692224082243513 1103 78-79 0.20924517109143181 1103 80-81 0.2687713761698127 1103 82-83 0.2597421955159511 1103 84-85 0.2824655664186366 1103 86-87 0.30983165442398075 1103 88-89 0.3869438693818985 1103 90-91 0.32466820300142274 1103 92-93 0.3384417514146172 1103 94-95 0.47564773073600364 1103 96-97 0.5550015065340119 1103 98-99 0.40647091358347254 1103 100-101 0.5081748146686493 1103 102-103 0.44349732365551375 1103 104-105 0.46120534090408327 1103 106-107 0.434269333881403 1103 108-109 0.3468717869607154 1103 110-111 0.3424006803478896 1103 112-113 0.4468714907210867 1103 114-115 0.38593066392234476 1103 116-117 0.4803959605579955 1103 118-119 0.5348712962106568 1103 120-121 0.438085391106533 1103 122-123 0.506728767656206 1103 124-125 0.4556739933453393 1104 1 0.39921240191559093 1104 2 0.4156656480783276 1104 3 0.5003435685089954 1104 4 0.5010858963954021 1104 5 0.22394808829158563 1104 6 0.28432853697965044 1104 7 0.24481062768838768 1104 8 0.2628220054309054 1104 9 0.5095929485081498 1104 10-11 0.5055313701560067 1104 12-13 0.5484246877051007 1104 14-15 0.5119332702811192 1104 16-17 0.48338483955446776 1104 18-19 0.4760246362406306 1104 20-21 0.5185017211688248 1104 22-23 0.49957617469162585 1104 24-25 0.6049307286857868 1104 26-27 0.512396412570908 1104 28-29 0.663753892944186 1104 30-31 0.518643778496866 1104 32-33 0.6927462672294666 1104 34-35 0.7027979416932908 1104 36-37 0.6518806778212678 1104 38-39 0.6853651772440443 1104 40-41 0.7113577416257684 1104 42-43 0.8315352637906557 1104 44-45 0.7971629773475399 1104 46-47 0.7478962979832033 1104 48-49 0.7391592882653129 1104 50-51 0.5765815970841004 1104 52-53 0.7209239092174471 1104 54-55 0.7185484807793614 1104 56-57 0.7280212199566733 1104 58-59 0.7228729134985059 1104 60-61 0.9025981676758121 1104 62-63 0.7601559214411253 1104 64-65 0.8489311377374875 1104 66-67 0.8525350167966934 1104 68-69 0.7374855738264259 1104 70-71 0.8189129693228878 1104 72-73 0.8567136903542902 1104 74-75 0.7019148561571669 1104 76-77 0.6986273146045221 1104 78-79 0.6579009220516028 1104 80-81 0.7686450369743412 1104 82-83 0.8363384912507428 1104 84-85 0.7575325261922394 1104 86-87 0.6532942327542841 1104 88-89 0.6495335702106821 1104 90-91 0.722712472255516 1104 92-93 0.6420443135734999 1104 94-95 0.7988107588338416 1104 96-97 0.7968494699461814 1104 98-99 0.7442861298761763 1104 100-101 0.8099865187317192 1104 102-103 0.909063575929622 1104 104-105 0.8432424340918736 1104 106-107 0.818643702185426 1104 108-109 0.7543599110763424 1104 110-111 0.7426527270428558 1104 112-113 0.612562187102732 1104 114-115 0.5948072557962085 1104 116-117 0.5971491695735622 1104 118-119 0.5304904846076646 1104 120-121 0.6878516635949055 1104 122-123 0.7941504373550146 1104 124-125 0.9159087315705285 1105 1 0.36785820067148123 1105 2 0.4871553642355586 1105 3 0.42523907219386103 1105 4 0.3798738306347289 1105 5 0.2889713115248469 1105 6 0.30948011656574437 1105 7 0.43027296784223523 1105 8 0.29323746909224724 1105 9 0.34771945520027003 1105 10-11 0.4371691323666411 1105 12-13 0.3116201723956209 1105 14-15 0.2982465861181893 1105 16-17 0.4052908391672574 1105 18-19 0.4224589522412998 1105 20-21 0.32051537733029534 1105 22-23 0.1756674251083865 1105 24-25 0.3947936803183012 1105 26-27 0.23422504410378764 1105 28-29 0.4637755223364479 1105 30-31 0.4603511024433189 1105 32-33 0.5848435972987502 1105 34-35 0.569114573305221 1105 36-37 0.4649511255936005 1105 38-39 0.6194910515234753 1105 40-41 0.443922200691496 1105 42-43 0.6855849923619104 1105 44-45 0.6411533916107928 1105 46-47 0.6039096899074252 1105 48-49 0.5521642256956127 1105 50-51 0.5005067059538675 1105 52-53 0.5675307113868229 1105 54-55 0.525931606120416 1105 56-57 0.6280469483200548 1105 58-59 0.6569634707499716 1105 60-61 0.6387594739007909 1105 62-63 0.6872213179635196 1105 64-65 0.5753482380594406 1105 66-67 0.7140693934401696 1105 68-69 0.6459248063965575 1105 70-71 0.6113108784084389 1105 72-73 0.5868681739882291 1105 74-75 0.5977784819240313 1105 76-77 0.3988254559407096 1105 78-79 0.41268985614710374 1105 80-81 0.45263632226895467 1105 82-83 0.45952501676152835 1105 84-85 0.5484187566868997 1105 86-87 0.5158469475357919 1105 88-89 0.39375136757731255 1105 90-91 0.46495983564723886 1105 92-93 0.472596873179679 1105 94-95 0.4707805303857384 1105 96-97 0.6113110060429285 1105 98-99 0.5938908536476113 1105 100-101 0.6489390413007641 1105 102-103 0.6337585594111346 1105 104-105 0.5727101570731605 1105 106-107 0.6766220383910877 1105 108-109 0.6392214591772216 1105 110-111 0.6875812201256579 1105 112-113 0.6006433306278396 1105 114-115 0.6569178306659289 1105 116-117 0.7668332157557884 1105 118-119 0.7392591353837403 1105 120-121 0.7011445903444624 1105 122-123 0.6700628701565918 1105 124-125 0.7954686997010292 1106 1 -0.05585884411237885 1106 2 -0.22959727229253346 1106 3 -0.019720518400990983 1106 4 -0.17977962575258744 1106 5 -0.22320247883377675 1106 6 -0.14446143476243378 1106 7 -0.2794906624373752 1106 8 -0.16978022447290186 1106 9 -0.07995986084257112 1106 10-11 0.07710751919629644 1106 12-13 -0.06595901578197783 1106 14-15 -0.11389425918112295 1106 16-17 -0.10531418281211558 1106 18-19 -0.19090658501062308 1106 20-21 -0.15611191284993708 1106 22-23 -0.33451562680076563 1106 24-25 -4.504925732668703 1106 26-27 -1.090460888282017 1106 28-29 -0.7779564496617297 1106 30-31 -0.6897891725962211 1106 32-33 -5.363372087947404 1106 34-35 -1.079469490888897 1106 36-37 -0.35155154214365325 1106 38-39 0.017013080761842048 1106 40-41 -0.5911123953141697 1106 42-43 -0.1818449384460763 1106 44-45 -0.1238041085240198 1106 46-47 -0.31584397559976196 1106 48-49 -0.1188760330191343 1106 50-51 -0.11119828212017069 1106 52-53 -0.07535149377520867 1106 54-55 -0.45766843938217505 1106 56-57 -0.2550065859239723 1106 58-59 -0.11703840844802471 1106 60-61 -0.12886520948315905 1106 62-63 -0.13038955213323078 1106 64-65 -0.052851070685257184 1106 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-0.34637578341016706 2216 6 -0.5247379018443752 2216 7 -0.49805557562810776 2216 8 -0.4251384046557902 2216 9 -1.1600280827503298 2216 10-11 -0.3182629312229963 2216 12-13 -0.556866679598734 2216 14-15 -0.8653313480561593 2216 16-17 -0.824037592876131 2216 18-19 -0.5302453572892958 2216 20-21 -0.46874130772555844 2216 22-23 -0.4856245740630172 2216 24-25 -0.6342345346295133 2216 26-27 -0.5811562117900664 2216 28-29 -0.6234792542525298 2216 30-31 -0.6175718544239857 2216 32-33 -0.6476877985518357 2216 34-35 -0.9175190541503753 2216 36-37 -0.897222529935604 2216 38-39 -0.6384909317116296 2216 40-41 -0.8238866669489937 2216 42-43 -0.6886098731741797 2216 44-45 -0.7259724064192739 2216 46-47 -0.8714885118561142 2216 48-49 -1.1161087837816552 2216 50-51 -0.8735064364430372 2216 52-53 -1.0597035297395934 2216 54-55 -0.8149230460293353 2216 56-57 -0.9600977481686499 2216 58-59 -0.7790696103396755 2216 60-61 -1.0843655060012267 2216 62-63 -1.0874399329048998 2216 64-65 -0.9080050249926543 2216 66-67 -0.7823152134881965 2216 68-69 -0.7601858398124293 2216 70-71 -0.8077611893989669 2216 72-73 -0.9414825053048332 2216 74-75 -0.8238837014907077 2216 76-77 -0.831615189019395 2216 78-79 -0.620133792389872 2216 80-81 -0.6580181128600024 2216 82-83 -0.7809324618934816 2216 84-85 -0.8601445910272396 2216 86-87 -0.7129344455104132 2216 88-89 -0.7146556458888931 2216 90-91 -0.6722794608224696 2216 92-93 -0.5698435190132578 2216 94-95 -0.6158722415651816 2216 96-97 -0.5792113096333438 2216 98-99 -0.42414834620222663 2216 100-101 -0.5722200756294775 2216 102-103 -0.4578962728165301 2216 104-105 -0.49938307661906123 2216 106-107 -0.52494781022531 2216 108-109 -0.5579887167203061 2216 110-111 -0.5430604046437288 2216 112-113 -0.47059812258892464 2216 114-115 -0.5770176194802339 2216 116-117 -0.47118464845140906 2216 118-119 -0.36584919179867725 2216 120-121 -0.2606011931926169 2216 122-123 -0.5648599049875713 2216 124-125 -0.6667725224356928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18518.0 3 3240.0 4 2269.0 5 1874.0 6 2381.0 7 2794.0 8 2978.0 9 3372.0 10 4092.0 11 4968.0 12 5159.0 13 5410.0 14 5352.0 15 4971.0 16 4492.0 17 3995.0 18 3655.0 19 3360.0 20 3287.0 21 3332.0 22 4034.0 23 5630.0 24 8479.0 25 12246.0 26 14507.0 27 14330.0 28 17016.0 29 20740.0 30 25396.0 31 31463.0 32 41296.0 33 54544.0 34 76868.0 35 109000.0 36 178881.0 37 248605.0 38 77145.0 39 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.040575198078784 17.873557804823808 9.640433224214798 21.445433772882602 2 18.578402382665658 21.39796129430718 38.29132884884115 21.73230747418601 3 17.628191122103846 30.48027182742571 29.936558710625956 21.954978339844487 4 10.754256537145709 16.06195175811274 36.78795924134442 36.395832463397134 5 10.678864942710119 44.777876074397696 33.39088702934559 11.152371953546593 6 29.96075811324309 36.32899481144764 20.25992287575733 13.450324199551938 7 28.801967054555274 34.900554787849245 21.09427120609305 15.203206951502432 8 28.978916426076395 33.44510705903031 19.946220426332765 17.629756088560526 9 30.076688442157373 13.14696465456212 18.519428483196425 38.25691842008408 10-11 24.730992229716442 27.598466239348816 28.443763161485265 19.226778369449477 12-13 25.259723738234985 26.491724188830666 28.034820169594894 20.213731903339458 14-15 23.26419725511237 27.269703539511475 25.52293920994045 23.94315999543571 16-17 22.615137129341935 29.829874448829045 25.89319920232253 21.661789219506485 18-19 21.888368045945533 28.812827691623426 27.66807381661991 21.630730445811132 20-21 23.831660890077117 27.552875459707437 27.396135495580886 21.21932815463456 22-23 23.506361023887436 28.21104495557708 26.755860807373992 21.52673321316149 24-25 22.804244750508015 28.088478430287182 27.132025724877824 21.975251094326982 26-27 22.24720126787809 28.225145471919454 27.07593673039722 22.451716529805235 28-29 22.405205515103606 28.225527650293174 27.099093569920583 22.270173264682633 30-31 22.75537954846607 27.734760596315734 27.456927924222864 22.05293193099534 32-33 22.422766635672165 28.157688376575923 27.162060786451114 22.257484201300798 34-35 22.649456864790114 28.2565291785869 27.30325065403462 21.790763302588363 36-37 22.7783037877574 28.000077687627762 26.939787233009472 22.281831291605368 38-39 22.69097784482687 28.10530031526779 27.21052828354582 21.99319355635952 40-41 22.647187637471752 28.00442240256682 27.376264497352288 21.972125462609142 42-43 22.324263853308654 27.78809307854109 27.223540767989245 22.66410230016101 44-45 22.228199911629464 28.035649256376516 27.35723545891984 22.37891537307418 46-47 22.309255043932907 28.04184891119415 26.993052696183156 22.655843348689782 48-49 22.381804860213865 28.036856791738344 27.08169158243665 22.49964676561114 50-51 22.20924180097033 27.754244694859754 27.478645426683602 22.55786807748632 52-53 22.42518762876186 27.88194719252408 27.394406169595907 22.298459009118147 54-55 21.83012730716623 27.867882944801835 27.75921616192613 22.542773586105803 56-57 22.372548876798664 28.074959395780073 27.125172674149013 22.427319053272253 58-59 22.714871356134406 27.772061783238154 27.081222205335898 22.431844655291542 60-61 22.71892347783781 27.878715487727483 27.376122894595394 22.026238139839315 62-63 22.332938741858516 27.74644891950237 27.558783285765475 22.361829052873638 64-65 22.028023978379448 27.910456892424552 27.62257455856262 22.438944570633378 66-67 22.350390063018956 28.478557580118657 26.852319863814184 22.318732493048202 68-69 22.278453846665535 28.02731876144978 27.351001705723178 22.343225686161503 70-71 21.876409606274056 27.835199213023948 27.59446455961533 22.693926621086664 72-73 22.041739458072634 28.524067048046696 26.95245244321785 22.48174105066282 74-75 21.84442425651979 28.406238877961403 27.253795391876913 22.495541473641893 76-77 21.866225272961838 27.98808664277457 27.345223708047 22.8004643762166 78-79 22.158124783325192 28.009097185873728 27.174249758926518 22.658528271874566 80-81 22.15207144147311 28.130614139522958 26.959571397773864 22.757743021230066 82-83 22.25204548003657 28.04226767032072 27.30127732344246 22.404409526200254 84-85 21.897262295973462 28.018883865103593 27.21579035277849 22.86806348614445 86-87 21.904378554802094 28.085522092818877 27.31263030835071 22.69746904402832 88-89 22.099190254897096 27.737836222413097 27.53143060794732 22.63154291474249 90-91 22.179116608992004 27.9666683013351 27.462818171621734 22.391396918051157 92-93 22.26339111004507 27.87304725622931 27.34297692006654 22.520584713659083 94-95 21.73913043478261 27.53009781334136 27.658233533297565 23.072538218578465 96-97 21.92962097251126 27.873635492300686 27.34725428179384 22.849489253394214 98-99 21.50453525143807 27.97648787370729 27.37305010407699 23.14592677077765 100-101 21.574530173073047 28.18679944744809 27.348066432471406 22.890603947007456 102-103 21.7882048129708 27.876813895290063 27.268613335741666 23.06636795599747 104-105 21.61285028555003 27.775992044802365 27.526906572069215 23.084251097578385 106-107 21.5765142646554 27.75831002301009 27.653820214408036 23.01135549792647 108-109 21.783427181651728 28.219219054453713 27.113917793825674 22.883435970068884 110-111 21.67258471516893 28.476989044529326 27.03655562293914 22.813870617362596 112-113 21.81929416106063 28.232440817951627 27.158994627428036 22.78927039355971 114-115 21.688293716785584 28.50915376529168 26.900441118990454 22.902111398932284 116-117 21.384514763855513 28.454598467703445 27.097439552750807 23.063447215690235 118-119 21.55550894243571 28.208026585016015 27.140052031895028 23.096412440653243 120-121 21.631832582755735 28.155472742501303 27.20175768684527 23.010936987897697 122-123 21.736835690913498 28.241387464209016 26.970270358193897 23.051506486683593 124-125 21.88865749923405 28.185169773594744 26.39651533330064 23.529657393870565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15.0 1 16.5 2 25.5 3 40.0 4 51.0 5 50.0 6 55.0 7 61.0 8 68.5 9 75.5 10 80.0 11 88.0 12 91.5 13 106.0 14 130.5 15 154.0 16 201.0 17 271.0 18 368.0 19 475.0 20 606.0 21 826.5 22 1189.5 23 1790.5 24 2528.0 25 3383.5 26 4727.0 27 6168.0 28 7767.5 29 9433.5 30 12001.5 31 15136.0 32 18096.0 33 21383.5 34 24662.5 35 27613.0 36 30843.0 37 33990.0 38 35042.0 39 35197.0 40 35205.5 41 35897.5 42 35490.0 43 35485.5 44 38120.5 45 40238.0 46 40387.0 47 39654.0 48 39261.5 49 38625.0 50 37537.0 51 35392.0 52 34085.0 53 33354.5 54 30850.5 55 27440.5 56 24605.0 57 22600.5 58 20621.0 59 18366.0 60 15938.0 61 13191.5 62 10476.0 63 7942.0 64 5836.5 65 4522.5 66 3481.0 67 2726.5 68 2070.0 69 1311.5 70 928.0 71 722.0 72 579.5 73 530.0 74 413.0 75 329.5 76 243.5 77 141.0 78 95.5 79 63.5 80 54.5 81 42.5 82 25.0 83 17.0 84 11.0 85 8.5 86 9.5 87 10.5 88 6.0 89 2.0 90 3.0 91 3.5 92 2.5 93 3.0 94 2.5 95 2.0 96 2.5 97 1.5 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.8843587554902983E-4 2 0.0 3 0.0 4 1.9421793777451492E-4 5 7.768717510980597E-4 6 3.8843587554902983E-4 7 7.768717510980597E-4 8 0.0011653076266470894 9 0.0019421793777451492 10-11 0.0014080800488652332 12-13 0.0022335062844069213 14-15 0.003738695302159412 16-17 0.00305893251994861 18-19 0.0029132690666177235 20-21 0.0038843587554902984 22-23 0.09385581842953433 24-25 0.002767605613286838 26-27 0.0018450704088578919 28-29 0.0016508524710833767 30-31 0.0014080800488652332 32-33 0.001796515924414263 34-35 8.739807199853171E-4 36-37 5.826538133235447E-4 38-39 0.0011167531422034607 40-41 0.0013595255644216044 42-43 0.0016508524710833767 44-45 0.0015537435021961193 46-47 0.0018450704088578919 48-49 1.4566345333088618E-4 50-51 0.0014566345333088618 52-53 0.0011167531422034607 54-55 0.0016994069555270055 56-57 0.0013109710799779758 58-59 9.225352044289459E-4 60-61 0.0010196441733162033 62-63 0.0013595255644216044 64-65 9.710896888725746E-4 66-67 1.4566345333088618E-4 68-69 2.4277242221814365E-4 70-71 6.312082977671735E-4 72-73 1.9421793777451492E-4 74-75 2.4277242221814365E-4 76-77 2.4277242221814365E-4 78-79 1.9421793777451492E-4 80-81 4.855448444362873E-4 82-83 4.855448444362873E-5 84-85 2.9132690666177236E-4 86-87 1.9421793777451492E-4 88-89 2.4277242221814365E-4 90-91 4.855448444362873E-5 92-93 1.9421793777451492E-4 94-95 3.398813911054011E-4 96-97 3.8843587554902983E-4 98-99 4.3699035999265857E-4 100-101 3.398813911054011E-4 102-103 0.0011653076266470894 104-105 3.8843587554902983E-4 106-107 5.34099328879916E-4 108-109 6.312082977671735E-4 110-111 0.0011167531422034607 112-113 3.398813911054011E-4 114-115 3.398813911054011E-4 116-117 9.225352044289459E-4 118-119 0.0017479614399706343 120-121 8.254262355416883E-4 122-123 7.283172666544309E-4 124-125 2.4277242221814365E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1029771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.54541758555101 #Duplication Level Percentage of deduplicated Percentage of total 1 53.68897821227213 18.010191938722183 2 14.942923404910536 10.025332111332553 3 9.975430845079249 10.038899798819081 4 6.596713596099696 8.851580490937847 5 4.242969482340088 7.116609154392373 6 2.8354902438247707 5.707062257331466 7 1.7909231390956115 4.2054085195211695 8 1.1905766669300586 3.195071316782583 9 0.8778116605396858 2.6501902842842737 >10 3.57534314303574 20.430377463894327 >50 0.18914454373245757 4.38300138657448 >100 0.09195459661170785 5.035480881083021 >500 0.001740465528265527 0.35079439632470005 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.855448444362873E-5 0.0 12-13 0.0 0.0 0.0 9.710896888725746E-5 0.0 14-15 0.0 0.0 0.0 9.710896888725746E-5 0.0 16-17 0.0 0.0 0.0 9.710896888725746E-5 0.0 18-19 0.0 0.0 0.0 9.710896888725746E-5 0.0 20-21 0.0 0.0 0.0 9.710896888725746E-5 0.0 22-23 0.0 0.0 0.0 9.710896888725746E-5 0.0 24-25 0.0 0.0 0.0 1.9421793777451492E-4 0.0 26-27 0.0 0.0 0.0 2.9132690666177236E-4 0.0 28-29 0.0 0.0 0.0 8.254262355416883E-4 0.0 30-31 0.0 0.0 0.0 0.0024277242221814365 0.0 32-33 0.0 0.0 0.0 0.00611786503989722 0.0 34-35 0.0 0.0 0.0 0.011992957657576296 0.0 36-37 0.0 0.0 0.0 0.023063380110723647 0.0 38-39 0.0 0.0 0.0 0.04937991067917041 0.0 40-41 0.0 0.0 0.0 0.07749295717203145 0.0 42-43 0.0 0.0 0.0 0.11191808664256422 0.0 44-45 0.0 0.0 0.0 0.15833617377067327 0.0 46-47 0.0 0.0 0.0 0.21509636608527527 0.0 48-49 0.0 0.0 0.0 0.27622646199980383 0.0 50-51 0.0 0.0 0.0 0.33954150971429575 0.0 52-53 0.0 0.0 0.0 0.4046045188687582 0.0 54-55 0.0 0.0 0.0 0.48510785407629464 0.0 56-57 0.0 0.0 0.0 0.5870722714079151 0.0 58-59 0.0 0.0 0.0 0.7081186011258814 0.0 60-61 0.0 0.0 0.0 0.8325151902704582 0.0 62-63 0.0 0.0 0.0 0.9688561825881676 0.0 64-65 0.0 0.0 0.0 1.1194236388478602 0.0 66-67 0.0 0.0 0.0 1.3063098494713874 0.0 68-69 0.0 0.0 0.0 1.5198039175700229 0.0 70-71 0.0 0.0 0.0 1.7463591419839943 0.0 72-73 0.0 0.0 0.0 1.9683987993447087 0.0 74-75 0.0 0.0 0.0 2.22612600277149 0.0 76-77 0.0 0.0 0.0 2.5270181428686573 0.0 78-79 0.0 0.0 0.0 2.882728295902681 0.0 80-81 0.0 0.0 0.0 3.2578602427141568 0.0 82-83 0.0 0.0 0.0 3.6259517892813062 0.0 84-85 0.0 0.0 0.0 4.033615240670013 0.0 86-87 0.0 0.0 0.0 4.472013680711537 0.0 88-89 0.0 0.0 0.0 4.97770863619193 0.0 90-91 0.0 0.0 0.0 5.524674903449407 0.0 92-93 0.0 0.0 0.0 6.067077049169184 0.0 94-95 0.0 0.0 0.0 6.649488090070511 0.0 96-97 0.0 0.0 0.0 7.269383192962319 0.0 98-99 0.0 4.855448444362873E-5 0.0 7.942833892195448 0.0 100-101 0.0 9.710896888725746E-5 0.0 8.67275345683652 0.0 102-103 0.0 9.710896888725746E-5 0.0 9.4266103823083 0.0 104-105 0.0 9.710896888725746E-5 0.0 10.211736395761775 0.0 106-107 0.0 9.710896888725746E-5 0.0 11.04245507010782 0.0 108-109 0.0 9.710896888725746E-5 0.0 11.87060035677835 0.0 110-111 0.0 9.710896888725746E-5 0.0 12.754874627465718 0.0 112-113 0.0 9.710896888725746E-5 0.0 13.654492115237272 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2230 0.0 68.56431 1 GTACATG 12860 0.0 53.248108 1 TACATGG 13105 0.0 51.662457 2 ACATGGG 13170 0.0 49.73606 3 CATGGGA 9215 0.0 47.581924 4 GTATCAA 3270 0.0 46.757923 2 CATGGGG 3490 0.0 46.19699 4 ATGGGAG 3225 0.0 43.90531 5 ATGGGGG 2045 0.0 40.438152 5 CGTATAG 105 3.2660682E-7 39.66236 1 ATGGGAT 3590 0.0 37.94992 5 CAACGCA 4155 0.0 37.944134 6 TCAACGC 4135 0.0 37.839905 5 AACGCAG 4210 0.0 37.589745 7 ATCAACG 4210 0.0 37.1658 4 ATGGGGA 1415 0.0 33.635925 5 TATCAAC 4720 0.0 33.276047 3 ATGGGAA 3480 0.0 32.482105 5 AGTACAT 7400 0.0 32.239067 2 GAGTACA 7685 0.0 32.127285 1 >>END_MODULE