FastQCFastQC Report
Thu 26 May 2016
SRR936205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029771
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.23519792264493755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.1613951062906219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.14731430580196958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32100.078.6648941
GTATCAA48250.052.4325182
GTACATG109650.047.6876641
TACATGG113000.045.7773322
ACATGGG111900.045.0038223
CATGGGG30500.043.71774
CCGAGTA853.4699606E-642.0185854
CATGGGA76450.040.1773534
ATGGGCG1655.456968E-1239.6842235
ATGGGGG19450.039.480195
ATCAACG66850.039.3577274
TCAACGC67300.039.0945635
ATGGGAG26450.038.9340485
CAACGCA68200.038.7306336
AACGCAG69100.038.1400877
CGTATAG957.4240525E-637.613851
TATCAAC71600.036.9961173
CGAGTAC1051.4790476E-534.015055
TATAACG1251.2734363E-633.3347432
TAACGCA1451.09335815E-732.8421134
Produced by FastQC (version 0.11.5)