##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936205_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029771 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.537693331818435 31.0 31.0 34.0 30.0 34.0 2 31.39360595705259 31.0 31.0 34.0 30.0 34.0 3 31.71635441277721 31.0 31.0 34.0 30.0 34.0 4 35.5832947325182 37.0 35.0 37.0 33.0 37.0 5 35.19116580288239 37.0 35.0 37.0 33.0 37.0 6 35.386447083866216 37.0 35.0 37.0 33.0 37.0 7 35.22490922739133 37.0 35.0 37.0 32.0 37.0 8 35.30612728460988 37.0 35.0 37.0 32.0 37.0 9 36.981354106883956 39.0 37.0 39.0 33.0 39.0 10-11 36.85872198770406 39.0 37.0 39.0 32.0 39.0 12-13 36.79874069089147 39.0 37.0 39.0 32.0 39.0 14-15 37.94144814720943 40.0 37.0 41.0 33.0 41.0 16-17 37.84692713234301 40.0 37.0 41.0 32.0 41.0 18-19 37.97261429968411 40.0 37.0 41.0 32.5 41.0 20-21 38.032853906353935 40.0 37.0 41.0 33.0 41.0 22-23 37.946073447397524 40.0 37.0 41.0 32.5 41.0 24-25 37.923611657349056 40.0 37.0 41.0 32.0 41.0 26-27 37.75827344137677 40.0 37.0 41.0 32.0 41.0 28-29 37.6231424268114 40.0 37.0 41.0 32.0 41.0 30-31 37.45429420715868 40.0 36.5 41.0 32.0 41.0 32-33 37.2778195346344 39.0 36.0 41.0 31.0 41.0 34-35 37.10342882058244 39.0 36.0 41.0 31.0 41.0 36-37 36.930132039065 39.0 35.5 41.0 31.0 41.0 38-39 36.92955763951403 39.0 35.5 41.0 30.5 41.0 40-41 36.96042955181298 40.0 36.0 41.0 31.0 41.0 42-43 36.86753608326511 40.0 36.0 41.0 31.0 41.0 44-45 36.695583775421916 40.0 36.0 41.0 30.0 41.0 46-47 36.41500586052627 40.0 35.5 41.0 30.0 41.0 48-49 36.15609635540329 39.0 35.0 41.0 29.5 41.0 50-51 35.97171458508736 39.0 35.0 41.0 28.5 41.0 52-53 35.82948976034477 39.0 35.0 41.0 28.0 41.0 54-55 35.65460476164118 39.0 35.0 41.0 28.0 41.0 56-57 35.472874066175876 39.0 35.0 41.0 27.5 41.0 58-59 35.26166545766 38.5 34.5 40.5 27.0 41.0 60-61 35.03700580031871 38.0 34.0 40.0 26.5 41.0 62-63 34.818250853830605 38.0 34.0 40.0 26.0 41.0 64-65 34.575048724425145 37.0 34.0 40.0 26.0 41.0 66-67 34.296113893283064 37.0 34.0 40.0 26.0 41.0 68-69 33.98967974433151 36.0 33.5 39.0 26.0 41.0 70-71 33.66836267480828 36.0 33.0 39.0 26.0 41.0 72-73 33.40007487101501 35.5 33.0 39.0 26.0 40.0 74-75 33.205231551480864 35.0 33.0 37.5 26.0 40.0 76-77 32.8991329140168 35.0 33.0 37.0 26.0 39.0 78-79 32.56843560364392 35.0 33.0 37.0 25.5 39.0 80-81 32.290134894068686 35.0 33.0 36.0 25.0 38.5 82-83 32.03422120063587 35.0 32.5 36.0 25.0 37.0 84-85 31.79502287401762 35.0 32.0 35.5 25.0 37.0 86-87 31.60777056258139 35.0 32.0 35.0 25.0 36.5 88-89 31.46177451103206 35.0 32.0 35.0 24.0 36.0 90-91 31.33743278845491 35.0 32.0 35.0 24.0 36.0 92-93 31.201962378043273 35.0 32.0 35.0 24.0 36.0 94-95 31.099420162346775 35.0 32.0 35.0 24.0 35.5 96-97 31.04563587438372 35.0 32.0 35.0 23.0 35.0 98-99 30.982188758471544 35.0 32.0 35.0 23.0 35.0 100-101 30.91359680938772 35.0 32.0 35.0 23.0 35.0 102-103 29.893671020061742 33.5 30.0 34.5 19.5 35.0 104-105 30.664380235994216 34.0 31.5 35.0 21.0 35.0 106-107 30.812563181522883 34.5 32.0 35.0 23.0 35.0 108-109 30.78549745525947 35.0 32.0 35.0 20.0 35.0 110-111 30.75634485725467 35.0 32.0 35.0 20.0 35.0 112-113 30.686295302547848 35.0 32.0 35.0 20.0 35.0 114-115 30.658522622990937 35.0 32.0 35.0 19.0 35.0 116-117 30.562602753427704 35.0 32.0 35.0 19.0 35.0 118-119 30.435664822567347 34.5 32.0 35.0 18.0 35.0 120-121 30.24110748894657 34.0 32.0 35.0 11.0 35.0 122-123 30.00724044472023 34.0 32.0 35.0 2.0 35.0 124-125 27.86077827012025 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12163425413654139 1101 2 0.7091468108333352 1101 3 0.4445150917768643 1101 4 0.20827483765097554 1101 5 0.5643442217218606 1101 6 0.3210245914207732 1101 7 0.4500255496668828 1101 8 0.4220551262662795 1101 9 0.47089209596783377 1101 10-11 0.43871070198331097 1101 12-13 0.39993139966559 1101 14-15 0.5291164375684971 1101 16-17 0.6340492775742064 1101 18-19 0.5124039241874598 1101 20-21 0.3605300174055657 1101 22-23 0.409463583597109 1101 24-25 0.33352099033874083 1101 26-27 0.37363788637965456 1101 28-29 0.34675273136801366 1101 30-31 0.2683488133086911 1101 32-33 0.18396739338794532 1101 34-35 0.24143079766947295 1101 36-37 0.2783944472758648 1101 38-39 0.16811335754824341 1101 40-41 0.2079236882363844 1101 42-43 0.111352622053694 1101 44-45 0.16186666141339145 1101 46-47 0.020897574611296932 1101 48-49 -0.03433756552882272 1101 50-51 -0.255776130667833 1101 52-53 -0.12062863769848775 1101 54-55 -0.1530587944850268 1101 56-57 -0.36537242466076947 1101 58-59 -0.3281305729755033 1101 60-61 -0.18442138268942898 1101 62-63 -0.07964974172136152 1101 64-65 -0.28986638864849823 1101 66-67 -0.21068521732281908 1101 68-69 -0.13563893553712347 1101 70-71 -0.17838063412668248 1101 72-73 -0.3201998016994452 1101 74-75 -0.11329444911948627 1101 76-77 -0.18583172939869286 1101 78-79 -0.1379840990673742 1101 80-81 -0.22018324857896232 1101 82-83 -0.16103261525874757 1101 84-85 -0.2188534715456001 1101 86-87 -0.21976074894468667 1101 88-89 -0.38024435851068006 1101 90-91 -0.3352856011322345 1101 92-93 -0.21248350699468332 1101 94-95 -0.3089884882960128 1101 96-97 -0.27459783093134504 1101 98-99 -0.21660466459969285 1101 100-101 -0.1510597531693456 1101 102-103 -0.10737158218669052 1101 104-105 -0.25740203993338895 1101 106-107 -0.2740138239138119 1101 108-109 -0.15595934982763993 1101 110-111 -0.2760152123358246 1101 112-113 -0.18446503126402547 1101 114-115 -0.31969004818428104 1101 116-117 -0.15646368621595386 1101 118-119 -0.2185116378751566 1101 120-121 -0.2200674995487546 1101 122-123 -0.26099857228734535 1101 124-125 -0.06489415345453864 1102 1 0.2582112648013748 1102 2 0.6941310793280344 1102 3 0.4911086680465431 1102 4 0.24949797673610874 1102 5 0.50035714832498 1102 6 0.392815344942413 1102 7 0.35871065963223714 1102 8 0.4527383507787164 1102 9 0.44944797869732156 1102 10-11 0.463435740099726 1102 12-13 0.46954728712810834 1102 14-15 0.5714766677023846 1102 16-17 0.6795212809261244 1102 18-19 0.5706372625641833 1102 20-21 0.4518187205941473 1102 22-23 0.49361743810687386 1102 24-25 0.45789706448437073 1102 26-27 0.45450926823042437 1102 28-29 0.5120848387689918 1102 30-31 0.42636780750103753 1102 32-33 0.4554743590890027 1102 34-35 0.3268226110794643 1102 36-37 0.4865439877452076 1102 38-39 0.3243324425216443 1102 40-41 0.2557495980838027 1102 42-43 0.2997146057363764 1102 44-45 0.2351135975524059 1102 46-47 0.3907836715297819 1102 48-49 0.42727329405924763 1102 50-51 0.42245581107709285 1102 52-53 0.4389937673790456 1102 54-55 0.47363452457162225 1102 56-57 0.4016569783179378 1102 58-59 0.42314656920549254 1102 60-61 0.5127428728476318 1102 62-63 0.4064904335559305 1102 64-65 0.39768186304007 1102 66-67 0.48133865732172154 1102 68-69 0.4936278715686271 1102 70-71 0.564140984569562 1102 72-73 0.6442485481413058 1102 74-75 0.5366950761420881 1102 76-77 0.5350690593103096 1102 78-79 0.4962227110644122 1102 80-81 0.4298113200751956 1102 82-83 0.3452545555144013 1102 84-85 0.4878014789321661 1102 86-87 0.4440552370579596 1102 88-89 0.42479670634346434 1102 90-91 0.38760752162006185 1102 92-93 0.3787190705668735 1102 94-95 0.3564497243256568 1102 96-97 0.2853004089246447 1102 98-99 0.3065646808843354 1102 100-101 0.39732539132385014 1102 102-103 0.4867693534790156 1102 104-105 0.5144352679812414 1102 106-107 0.49773134509803185 1102 108-109 0.5740145021216279 1102 110-111 0.65050197593402 1102 112-113 0.47367636218654496 1102 114-115 0.53749251874736 1102 116-117 0.456592881251062 1102 118-119 0.4799730166330569 1102 120-121 0.4010090319937838 1102 122-123 0.5439456982247144 1102 124-125 0.6438113240577508 1103 1 0.1738722053918096 1103 2 0.6612839741098604 1103 3 0.38347995342245866 1103 4 0.17734209072042972 1103 5 0.3966925816265672 1103 6 0.29038160992910633 1103 7 0.2927974481458975 1103 8 0.2722303599111342 1103 9 0.29266917128394 1103 10-11 0.30858025026991953 1103 12-13 0.3945295076003532 1103 14-15 0.5498421110154439 1103 16-17 0.5998930333007948 1103 18-19 0.47019437681711196 1103 20-21 0.32956233827783876 1103 22-23 0.40706127921740887 1103 24-25 0.3348661662595731 1103 26-27 0.4451727269514052 1103 28-29 0.3744064330355883 1103 30-31 0.28459858424510287 1103 32-33 0.3895954857463835 1103 34-35 0.30286450666709186 1103 36-37 0.17317408810416168 1103 38-39 0.2453020601320759 1103 40-41 0.49427607966024567 1103 42-43 0.4816052791913137 1103 44-45 0.5295956104696486 1103 46-47 0.5270255982506598 1103 48-49 0.48488430546256467 1103 50-51 0.47124191954289785 1103 52-53 0.4479048972108046 1103 54-55 0.5002351399111618 1103 56-57 0.4979418963947566 1103 58-59 0.5339279069585317 1103 60-61 0.36946124512805056 1103 62-63 0.4280158712528035 1103 64-65 0.5814370976993146 1103 66-67 0.32763540298137883 1103 68-69 0.2989611194381325 1103 70-71 0.3833365410611478 1103 72-73 0.5847691601843792 1103 74-75 0.4702507680327912 1103 76-77 0.5296707215816951 1103 78-79 0.520089247093523 1103 80-81 0.5344518531252689 1103 82-83 0.5213352150509465 1103 84-85 0.4545362114303906 1103 86-87 0.4575608105202278 1103 88-89 0.37416167960549984 1103 90-91 0.36728277434879786 1103 92-93 0.2349589980447142 1103 94-95 0.4276731389717163 1103 96-97 0.27369106406590404 1103 98-99 0.3576411435366431 1103 100-101 0.4700043617289502 1103 102-103 0.4352995283135819 1103 104-105 0.49407427489160227 1103 106-107 0.38953643263740645 1103 108-109 0.4724880571725407 1103 110-111 0.3720474823682274 1103 112-113 0.32339471310100976 1103 114-115 0.3856717577530482 1103 116-117 0.42954052855406744 1103 118-119 0.2978435023117605 1103 120-121 0.496918138164272 1103 122-123 0.5450635955704541 1103 124-125 0.6078306724571334 1104 1 0.1847500101219488 1104 2 0.35858789567848603 1104 3 0.3095778410311141 1104 4 0.1876513690709416 1104 5 0.4912195676787192 1104 6 0.1300129521567186 1104 7 0.1742975997529328 1104 8 0.27481399645854054 1104 9 0.475545156729666 1104 10-11 0.2107968714544768 1104 12-13 0.2727859003636439 1104 14-15 0.1809316602371993 1104 16-17 0.4283086134503762 1104 18-19 0.3350240715816142 1104 20-21 0.2843171138994336 1104 22-23 0.2825066248814565 1104 24-25 0.3064809075169066 1104 26-27 0.07872904896474608 1104 28-29 0.30310690807096563 1104 30-31 0.12690200045695121 1104 32-33 0.20141325409083777 1104 34-35 0.2521203688057625 1104 36-37 0.14812102564205532 1104 38-39 0.15177947068392683 1104 40-41 0.09850591592464042 1104 42-43 0.3344643351848475 1104 44-45 0.2364097149774338 1104 46-47 0.09465812806671181 1104 48-49 0.1993008710578792 1104 50-51 0.13390363371310343 1104 52-53 0.26303275247662583 1104 54-55 0.24940130122104165 1104 56-57 0.3276935135364525 1104 58-59 0.40943642222030263 1104 60-61 0.3137077581662595 1104 62-63 0.17390353044019236 1104 64-65 0.1943116934138871 1104 66-67 0.18087031808143905 1104 68-69 0.11633781238335672 1104 70-71 0.10970163606822325 1104 72-73 0.06262505769802829 1104 74-75 0.11797287233842724 1104 76-77 0.25487981295020745 1104 78-79 0.19348347591906645 1104 80-81 0.12530868554896557 1104 82-83 0.03258572040773089 1104 84-85 0.04498244146880381 1104 86-87 0.1297800848278854 1104 88-89 0.06664576086686935 1104 90-91 0.09771599145007315 1104 92-93 0.1163454443758205 1104 94-95 0.0712576346256455 1104 96-97 0.06933741538993843 1104 98-99 0.16013192077537397 1104 100-101 -0.02729513796783678 1104 102-103 0.03445684587978448 1104 104-105 0.13441817018167868 1104 106-107 0.2254407685985953 1104 108-109 0.09168908810037735 1104 110-111 0.2994308712908058 1104 112-113 0.3715710068143707 1104 114-115 0.21909037004332532 1104 116-117 0.25926056089690164 1104 118-119 0.36136684979508615 1104 120-121 0.3413345774203904 1104 122-123 0.46524880883101716 1104 124-125 0.4293228646948535 1105 1 0.27179678560409926 1105 2 0.745360589064834 1105 3 0.3932916406199709 1105 4 0.24425962417919322 1105 5 0.4721966818786214 1105 6 0.3550556162244476 1105 7 0.5019935058689313 1105 8 0.4005810438781481 1105 9 0.346063416731063 1105 10-11 0.21109294892210073 1105 12-13 0.34245523416738877 1105 14-15 0.44196347374279554 1105 16-17 0.4578018086093465 1105 18-19 0.4841495817113781 1105 20-21 0.40048305342569535 1105 22-23 0.4712358816172113 1105 24-25 0.3971267260356868 1105 26-27 0.4440849842200336 1105 28-29 0.4829717737765975 1105 30-31 0.2562680909131956 1105 32-33 0.339724617060952 1105 34-35 0.20898729827015927 1105 36-37 0.18152324852874102 1105 38-39 0.13019436195322953 1105 40-41 0.036853913449157005 1105 42-43 0.05612388415894998 1105 44-45 0.16699528594031676 1105 46-47 0.15332227337816562 1105 48-49 0.22368663840539682 1105 50-51 0.32841573354647124 1105 52-53 0.2636117321475595 1105 54-55 0.2699420872646314 1105 56-57 0.26538164990629554 1105 58-59 0.2111915138195002 1105 60-61 0.0697013139980811 1105 62-63 0.1838012647705014 1105 64-65 0.1718292027911943 1105 66-67 0.24634424514830044 1105 68-69 0.21579715077281492 1105 70-71 0.18218992746700735 1105 72-73 0.14601057361758762 1105 74-75 0.28316012761246867 1105 76-77 0.21300150335125778 1105 78-79 0.24576914639180103 1105 80-81 0.22661603774903227 1105 82-83 0.12355049011691221 1105 84-85 0.18378275313699888 1105 86-87 0.3171134955048984 1105 88-89 0.3352592070292637 1105 90-91 0.2599168534783409 1105 92-93 0.17285726386884548 1105 94-95 0.3186976990673287 1105 96-97 0.21588002474371848 1105 98-99 0.14846376030211772 1105 100-101 0.19217512125183234 1105 102-103 0.2673404391257037 1105 104-105 0.2704659495457733 1105 106-107 0.17497987223412537 1105 108-109 0.1856678825124547 1105 110-111 0.2715426795116578 1105 112-113 0.26043783526840514 1105 114-115 0.2717586721506713 1105 116-117 0.3330233761288248 1105 118-119 0.40481393079697625 1105 120-121 0.37941548305878214 1105 122-123 0.35970506018285064 1105 124-125 0.4112143788364868 1106 1 0.09405634391870166 1106 2 0.5247265665691181 1106 3 0.22851094317454113 1106 4 0.15927023529527418 1106 5 0.30049235122841367 1106 6 0.23753778267972336 1106 7 0.4225471601482056 1106 8 0.33028064550885006 1106 9 0.26664587947027485 1106 10-11 0.2594230185277695 1106 12-13 0.18835878196615852 1106 14-15 0.2053008673454002 1106 16-17 0.1718785824164044 1106 18-19 0.18845013077108064 1106 20-21 0.14521793803304206 1106 22-23 0.0874817108539574 1106 24-25 0.13902360800004487 1106 26-27 0.16770382170264497 1106 28-29 0.21742443889154117 1106 30-31 0.26808971631604805 1106 32-33 0.23211024562830573 1106 34-35 0.18117273020130398 1106 36-37 0.2865686809474113 1106 38-39 0.14470311492363663 1106 40-41 0.11486611493995724 1106 42-43 0.2479933980863649 1106 44-45 0.2765562597844493 1106 46-47 0.19046616572612152 1106 48-49 0.18479595616935995 1106 50-51 0.2212584194109013 1106 52-53 0.20486334154325192 1106 54-55 0.2916258087858026 1106 56-57 0.14327348374205684 1106 58-59 0.10974067545638633 1106 60-61 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66-67 -0.747542042532146 2216 68-69 -0.5312879876396437 2216 70-71 -0.4513623710315642 2216 72-73 -0.5329405398666438 2216 74-75 -0.5002531863370621 2216 76-77 -0.30237448032806213 2216 78-79 -0.2793563210965857 2216 80-81 -0.24402370996241984 2216 82-83 -0.33645097563350745 2216 84-85 -0.3471120501047018 2216 86-87 -0.32924261903003327 2216 88-89 -0.3938357145194402 2216 90-91 -0.44761695412995195 2216 92-93 -0.34275484771663756 2216 94-95 -0.39867626696165814 2216 96-97 -0.45410888490996726 2216 98-99 -0.40967175281148727 2216 100-101 -0.6204780440586433 2216 102-103 -0.5713039959957662 2216 104-105 -0.4289407954488027 2216 106-107 -0.5105041133207564 2216 108-109 -0.5456482865842922 2216 110-111 -0.596412090122481 2216 112-113 -0.4912954519984858 2216 114-115 -0.549419402316925 2216 116-117 -0.7452991099035451 2216 118-119 -0.7108305992991433 2216 120-121 -0.7057922908028509 2216 122-123 -0.9300416416917372 2216 124-125 -1.2302417544690272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 36.0 4 90.0 5 199.0 6 451.0 7 997.0 8 1524.0 9 2574.0 10 4221.0 11 5309.0 12 6069.0 13 7531.0 14 10610.0 15 7747.0 16 3713.0 17 3167.0 18 3033.0 19 2791.0 20 2801.0 21 2931.0 22 3430.0 23 4063.0 24 5340.0 25 7080.0 26 9462.0 27 12285.0 28 16167.0 29 20525.0 30 26171.0 31 32819.0 32 41568.0 33 53125.0 34 73167.0 35 101303.0 36 168060.0 37 278191.0 38 110902.0 39 303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.82467606925811 18.47070873150529 10.937557697278535 21.767057501958064 2 18.519669440748903 21.73864066733348 37.5427522650689 22.198937626848714 3 17.76831936420816 28.839227362199942 31.943898206494453 21.44855506709744 4 11.431667817407948 16.366648507289487 37.190792904441864 35.01089077086071 5 10.46009614123258 43.39347261859994 34.35091064034881 11.79552059981868 6 30.23895603974087 36.853630564465305 18.9896588659032 13.917754529890628 7 27.045527597883414 35.002442290567515 21.965757435390977 15.986272676158098 8 28.389321509345283 32.936643195428886 20.402885690119454 18.27114960510638 9 28.934588369647233 11.559657438401354 19.34080489739952 40.164949294551896 10-11 24.65251983207917 26.224179939034993 29.265729953552782 19.85757027533306 12-13 25.728729979772204 25.61860840905405 29.116570577341953 19.53609103383179 14-15 23.085909391505492 26.43107059724929 27.110881933944537 23.37213807730068 16-17 21.7062062402284 28.899899977664916 27.523476116025908 21.870417666080773 18-19 20.909357517350948 27.318063919065498 30.555822605219994 21.21675595836356 20-21 22.48446499270226 26.265257032874302 30.540333724682476 20.70994424974096 22-23 22.148516514836793 26.756822633381596 30.220262563230076 20.874398288551532 24-25 21.634810069423203 26.9656069164892 30.248181391785163 21.151401622302433 26-27 21.24443201449643 26.862719963953154 30.35126256225899 21.541585459291433 28-29 21.22253394201235 26.956138792022692 30.458422309426076 21.36290495653888 30-31 21.77110250725647 26.40635636466748 30.585732167637268 21.236808960438776 32-33 21.460839351661683 26.928851171765373 30.12281371295171 21.48749576362123 34-35 21.72033560892238 27.067063519038232 30.288171145012967 20.92442972702642 36-37 22.013486493599064 26.763765924657037 29.806821128192578 21.41592645355132 38-39 21.80844090579362 26.907875634485723 29.85445307743178 21.42923038228888 40-41 21.784309327025134 26.88461803643723 29.744525724651403 21.586546911886234 42-43 21.67736754117162 26.687882285363017 29.465513047168496 22.169237126296863 44-45 21.546546291354936 27.097944629752195 29.40664883892243 21.948860239970436 46-47 21.944815523250252 27.11717657022179 28.600293981822077 22.337713924705884 48-49 22.176765570090353 27.308484380258037 28.234602294220164 22.280147755431447 50-51 22.091194395926113 27.06434380795189 28.433459149181857 22.41100264694014 52-53 22.20399137414896 27.301226430532132 28.280156609405406 22.214625585913506 54-55 21.602235216310785 27.4347649167378 28.53372684638909 22.42927302056232 56-57 22.087572399165587 27.522074219099007 27.937920264774093 22.452433116961313 58-59 22.525354984347814 27.060822342976813 28.05283140167529 22.360991271000074 60-61 22.529254673464433 27.014081087642634 28.43355183296917 22.02311240592377 62-63 22.188703101352207 27.01011545888583 28.5277013441475 22.273480095614463 64-65 21.800960523932737 27.06068120384501 28.777116398583928 22.361241873638324 66-67 22.020532297606152 27.537440148930294 28.18444593240938 22.257581621054175 68-69 22.090087937923432 27.187875238951193 28.295948426842944 22.426088396282438 70-71 21.817547945145616 27.028889205040667 28.44234662093364 22.711216228880076 72-73 21.897293118885628 27.738248072825726 27.793455861930997 22.571002946357645 74-75 21.585813032525962 27.595502650126924 28.25050836989518 22.568175947451934 76-77 21.571700686613966 27.193331284319168 28.39996756536817 22.835000463698705 78-79 22.007449239854164 27.18788039459316 27.958052748697643 22.846617616855035 80-81 21.858137880638107 27.412255679073187 27.713051732551282 23.016554707737427 82-83 22.08615617378452 27.202786643030308 28.026223344156143 22.684833839029025 84-85 21.675481746157757 27.11296071596605 27.999809666143733 23.21174787173246 86-87 21.577880443886606 27.151371547460958 28.057546970554032 23.213201038098404 88-89 21.78161190220814 27.01274133692511 28.025114626390558 23.180532134476188 90-91 21.868221988833263 26.971865624282295 28.160498448902143 22.999413937982304 92-93 21.845270898528067 26.975943598919173 28.012439701194687 23.166345801358073 94-95 21.56137276580331 26.660034056462646 28.01831105388097 23.76028212385307 96-97 21.623094025717755 26.822532144766402 27.977369385256985 23.577004444258858 98-99 21.22888752394386 26.79449673586614 27.895295228707866 24.081320511482126 100-101 21.366722909408722 26.918974035275124 27.884633592697345 23.829669462618806 102-103 21.48062224026305 26.48031634463464 27.829461730514694 24.20959968458762 104-105 21.48690307346971 26.275182335378624 28.104532638287687 24.13338195286398 106-107 21.378480225333075 26.130731832375265 28.303850055036854 24.186937887254807 108-109 21.52853914646214 26.492506513647342 27.634276302947963 24.34467803694255 110-111 21.46520950431361 26.678248608419015 27.591030566226827 24.26551132104055 112-113 21.456018328549348 26.364771347170713 27.752280992433842 24.426929331846097 114-115 21.348650198802282 26.425041074149096 27.485756995475164 24.740551731573454 116-117 20.958438029043165 26.500357916064072 27.726499678021234 24.814704376871532 118-119 20.97158521158735 26.3172948286202 27.707854310778096 25.003265649014356 120-121 20.986800671838665 26.21039970904472 27.7749641525806 25.027835466536015 122-123 21.153837749699793 26.15528718480856 27.400684560675494 25.290190504816156 124-125 20.971352378658644 26.003987495617736 27.119825894248816 25.904834231474798 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 1.0 2 4.5 3 12.0 4 22.5 5 31.0 6 49.5 7 109.5 8 189.0 9 269.5 10 356.0 11 436.0 12 541.0 13 647.0 14 776.0 15 940.0 16 1085.0 17 1215.5 18 1375.5 19 1524.5 20 1760.0 21 2067.5 22 2395.5 23 2888.0 24 3550.5 25 4336.5 26 5633.5 27 7193.5 28 8463.5 29 9940.0 30 12638.0 31 15539.5 32 18176.0 33 21342.5 34 24650.5 35 27003.0 36 30137.5 37 33694.5 38 34693.5 39 34837.0 40 34500.0 41 34774.0 42 34769.0 43 35330.0 44 37852.0 45 38981.0 46 38844.0 47 38807.0 48 38660.0 49 38363.5 50 37687.5 51 35095.5 52 33295.0 53 32678.5 54 30456.0 55 27401.5 56 24767.5 57 22326.5 58 19909.0 59 17626.0 60 15043.5 61 12423.5 62 10032.0 63 7690.5 64 5607.0 65 4278.0 66 3241.5 67 2477.5 68 1831.5 69 1116.5 70 742.0 71 574.0 72 505.0 73 502.5 74 376.5 75 269.0 76 181.5 77 97.0 78 62.0 79 29.5 80 11.0 81 5.5 82 7.0 83 5.0 84 4.0 85 4.5 86 2.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06729651543886941 2 9.710896888725746E-5 3 0.0 4 0.0 5 0.06409191946558992 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.710896888725746E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 9.710896888725746E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0025733876755123226 44-45 0.009953669310943889 46-47 0.011216085906478237 48-49 0.009371015497620344 50-51 0.008885470653184057 52-53 0.007331727150987937 54-55 0.006991845759882537 56-57 0.00611786503989722 58-59 0.004127131177708442 60-61 0.0020392883466324066 62-63 0.0010196441733162033 64-65 0.001990733862188778 66-67 0.00267049664439958 68-69 0.0010196441733162033 70-71 0.0025733876755123226 72-73 0.0024277242221814365 74-75 8.739807199853171E-4 76-77 7.283172666544309E-4 78-79 1.4566345333088618E-4 80-81 3.398813911054011E-4 82-83 1.4566345333088618E-4 84-85 1.4566345333088618E-4 86-87 3.398813911054011E-4 88-89 0.0012138621110907182 90-91 0.0016022979866397481 92-93 3.398813911054011E-4 94-95 0.0010196441733162033 96-97 0.0015051890177524905 98-99 0.001796515924414263 100-101 8.254262355416883E-4 102-103 7.768717510980597E-4 104-105 6.312082977671735E-4 106-107 6.312082977671735E-4 108-109 9.710896888725746E-4 110-111 6.797627822108022E-4 112-113 0.011701630750914524 114-115 0.022383617328512843 116-117 0.019421793777451492 118-119 0.01102186796870372 120-121 0.0057779836487918184 122-123 0.0057779836487918184 124-125 0.005049666382137388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1029771.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.26575176284279 #Duplication Level Percentage of deduplicated Percentage of total 1 44.67834943787592 13.075454838224235 2 17.57381967484535 10.286220882579734 3 11.892936044208536 10.441671420035176 4 8.01283343070705 9.380063764003218 5 5.177324128271176 7.575914136688035 6 3.392755942512123 5.9574931923281715 7 2.148002566584446 4.400403692972671 8 1.4850718072152846 3.476939428796708 9 1.0029344375776996 2.6416467256099785 >10 4.27354718561772 21.317286340398308 >50 0.24251112736142486 4.8604157174886575 >100 0.11757605700740614 5.791747326816316 >500 0.001336091551988584 0.2472206480485453 >1k 0.001002068663991438 0.547521886010242 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2422 0.23519792264493755 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1662 0.1613951062906219 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1517 0.14731430580196958 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.855448444362873E-5 0.0 12-13 0.0 0.0 0.0 9.710896888725746E-5 0.0 14-15 0.0 0.0 0.0 9.710896888725746E-5 0.0 16-17 0.0 0.0 0.0 9.710896888725746E-5 0.0 18-19 0.0 0.0 0.0 9.710896888725746E-5 0.0 20-21 0.0 0.0 0.0 9.710896888725746E-5 0.0 22-23 0.0 0.0 0.0 9.710896888725746E-5 0.0 24-25 0.0 0.0 0.0 1.9421793777451492E-4 0.0 26-27 0.0 0.0 0.0 2.9132690666177236E-4 0.0 28-29 0.0 0.0 0.0 7.28317266654431E-4 0.0 30-31 0.0 0.0 0.0 0.0023306152532941793 0.0 32-33 0.0 0.0 0.0 0.006214974008784477 0.0 34-35 0.0 0.0 0.0 0.011992957657576296 0.0 36-37 0.0 0.0 0.0 0.023597479439603563 0.0 38-39 0.0 0.0 0.0 0.0498654555236067 0.0 40-41 0.0 0.0 0.0 0.07783283856313686 0.0 42-43 0.0 0.0 0.0 0.1128891763314368 0.0 44-45 0.0 0.0 0.0 0.15984136278842576 0.0 46-47 0.0 0.0 0.0 0.21694143649413317 0.0 48-49 0.0 0.0 0.0 0.2778287599864436 0.0 50-51 0.0 0.0 0.0 0.3412409166698227 0.0 52-53 0.0 0.0 0.0 0.4063039258242852 0.0 54-55 0.0 0.0 0.0 0.48603038928072356 0.0 56-57 0.0 0.0 0.0 0.5890144507856601 0.0 58-59 0.0 0.0 0.0 0.7114688605524917 0.0 60-61 0.0 0.0 0.0 0.8363509945415049 0.0 62-63 0.0 0.0 0.0 0.9725948778903271 0.0 64-65 0.0 0.0 0.0 1.1242305328077795 0.0 66-67 0.0 0.0 0.0 1.3128161503868336 0.0 68-69 0.0 0.0 0.0 1.5267957633299054 0.0 70-71 0.0 0.0 0.0 1.7543220774327497 0.0 72-73 0.0 0.0 0.0 1.9788380135000887 0.0 74-75 0.0 0.0 0.0 2.2376819700690733 0.0 76-77 0.0 0.0 0.0 2.5383313377440224 0.0 78-79 0.0 0.0 0.0 2.895983670155792 0.0 80-81 0.0 0.0 0.0 3.271406943873929 0.0 82-83 0.0 0.0 0.0 3.6389643911121987 0.0 84-85 0.0 0.0 0.0 4.0470162783764545 0.0 86-87 0.0 0.0 0.0 4.488425096453484 0.0 88-89 0.0 0.0 0.0 4.997615974813818 0.0 90-91 0.0 0.0 0.0 5.548515155311229 0.0 92-93 0.0 0.0 0.0 6.0934906887065186 0.0 94-95 0.0 0.0 0.0 6.678086681407808 0.0 96-97 0.0 0.0 0.0 7.301963252023993 0.0 98-99 0.0 0.0 0.0 7.978618547230404 0.0 100-101 0.0 0.0 0.0 8.713781996191386 0.0 102-103 0.0 0.0 0.0 9.471571834903099 0.0 104-105 0.0 0.0 0.0 10.263009931334249 0.0 106-107 0.0 0.0 0.0 11.101691541129046 0.0 108-109 0.0 0.0 0.0 11.937120000466123 0.0 110-111 0.0 0.0 0.0 12.829648533508905 0.0 112-113 0.0 0.0 0.0 13.734315687662598 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3210 0.0 78.664894 1 GTATCAA 4825 0.0 52.432518 2 GTACATG 10965 0.0 47.687664 1 TACATGG 11300 0.0 45.777332 2 ACATGGG 11190 0.0 45.003822 3 CATGGGG 3050 0.0 43.7177 4 CCGAGTA 85 3.4699606E-6 42.018585 4 CATGGGA 7645 0.0 40.177353 4 ATGGGCG 165 5.456968E-12 39.684223 5 ATGGGGG 1945 0.0 39.48019 5 ATCAACG 6685 0.0 39.357727 4 TCAACGC 6730 0.0 39.094563 5 ATGGGAG 2645 0.0 38.934048 5 CAACGCA 6820 0.0 38.730633 6 AACGCAG 6910 0.0 38.140087 7 CGTATAG 95 7.4240525E-6 37.61385 1 TATCAAC 7160 0.0 36.996117 3 CGAGTAC 105 1.4790476E-5 34.01505 5 TATAACG 125 1.2734363E-6 33.334743 2 TAACGCA 145 1.09335815E-7 32.842113 4 >>END_MODULE