FastQCFastQC Report
Thu 26 May 2016
SRR936204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715306
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22460.13093873629544817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG169350.054.030191
TACATGG176150.050.763832
ACATGGG178950.048.5399323
GGTATCA34600.047.9726261
CATGGGA129700.047.6140824
GCGTAAT505.336468E-447.5943451
ATGGGAT47300.046.4133875
TACCTGG16700.044.5319332
ATGGGAG42150.040.6511575
CATGGGG44550.037.6599244
ATGGGAC24900.037.51275
GTATCAA46500.035.8247452
CGTATAG1902.910383E-1134.4432751
TGGGATA12750.030.3306146
AGTACAT97300.030.1447812
ATGGGCG4150.030.1057345
GTCGGGA7750.029.939252
TCAACGC57100.029.3826545
CATGGGT16000.029.375494
GAGTACA104400.029.1196231
Produced by FastQC (version 0.11.5)