FastQCFastQC Report
Thu 26 May 2016
SRR936204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715306
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104590.6097454331763545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78000.454729360242429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76420.44551817576572345No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.15437478793871182No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.11712195958038973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64000.073.550291
GTATCAA98550.047.787572
GTACATG143050.045.3187941
ACATGGG148600.042.990853
TACATGG149800.042.9246752
TACCTGG13700.041.7201422
ATGGGAT39250.040.6527755
CATGGGA107600.039.95034
TATCACG1850.038.619322
TATAACG2200.037.8878942
ATCACGC1900.037.603023
CATGGGG39750.036.2471054
ATGGGAG34750.035.8085375
TCAACGC137250.035.7879375
ATCAACG138450.035.477754
CAACGCA140450.035.02776
AACGCAG142800.034.451267
TATCAAC146100.033.8238453
TAACGCA2500.033.3413474
ATATAGG7750.033.0340542
Produced by FastQC (version 0.11.5)