##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936204_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1715306 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59075406953628 31.0 31.0 34.0 30.0 34.0 2 31.445988645757666 31.0 31.0 34.0 30.0 34.0 3 31.749853961916997 31.0 31.0 34.0 30.0 34.0 4 35.61734640932872 37.0 35.0 37.0 33.0 37.0 5 35.21493832587305 37.0 35.0 37.0 33.0 37.0 6 35.427060244644395 37.0 35.0 37.0 33.0 37.0 7 35.278784659996525 37.0 35.0 37.0 32.0 37.0 8 35.35601985884734 37.0 35.0 37.0 33.0 37.0 9 37.04076532117301 39.0 37.0 39.0 33.0 39.0 10-11 36.92041682358716 39.0 37.0 39.0 33.0 39.0 12-13 36.871077522028145 39.0 37.0 39.0 32.0 39.0 14-15 38.01339877549545 40.0 37.0 41.0 33.0 41.0 16-17 37.93052143465947 40.0 37.0 41.0 32.5 41.0 18-19 38.070278422625464 40.0 37.0 41.0 33.0 41.0 20-21 38.116635748956746 40.0 37.0 41.0 33.0 41.0 22-23 37.998271153951535 40.0 37.0 41.0 33.0 41.0 24-25 37.95040185249746 40.0 37.0 41.0 33.0 41.0 26-27 37.76299389146892 40.0 37.0 41.0 32.0 41.0 28-29 37.595647365542945 40.0 36.5 41.0 32.0 41.0 30-31 37.37463111538116 39.5 36.0 41.0 32.0 41.0 32-33 37.08985248113164 39.0 35.5 41.0 31.0 41.0 34-35 36.826102164861545 39.0 35.0 41.0 30.5 41.0 36-37 36.57693000549173 39.0 35.0 41.0 30.0 41.0 38-39 36.52451020401024 39.0 35.0 41.0 30.0 41.0 40-41 36.503046978206804 39.0 35.0 41.0 30.0 41.0 42-43 36.351170578310814 39.0 35.0 41.0 30.0 41.0 44-45 36.10955129871871 39.0 35.0 41.0 30.0 41.0 46-47 35.706726963002524 39.0 35.0 41.0 27.5 41.0 48-49 35.33952834071589 39.0 35.0 41.0 26.0 41.0 50-51 35.119898723609666 39.0 35.0 41.0 24.5 41.0 52-53 34.97338871315089 39.0 35.0 41.0 24.0 41.0 54-55 34.77958480877464 39.0 34.5 41.0 24.0 41.0 56-57 34.57036791103162 38.0 34.0 40.5 23.5 41.0 58-59 34.34255491440011 38.0 34.0 40.0 23.0 41.0 60-61 34.1066045941657 38.0 34.0 40.0 23.0 41.0 62-63 33.88417751701446 37.0 34.0 40.0 22.5 41.0 64-65 33.61588311356691 37.0 33.0 40.0 22.0 41.0 66-67 33.329451130002454 36.0 33.0 39.5 22.0 41.0 68-69 33.023949954118976 36.0 33.0 39.0 21.5 41.0 70-71 32.68924757448525 35.0 33.0 39.0 20.0 41.0 72-73 32.41849908995829 35.0 32.5 38.5 20.0 40.0 74-75 32.232053639408946 35.0 33.0 37.0 20.0 39.5 76-77 31.940903547238804 35.0 32.0 37.0 20.0 39.0 78-79 31.616406343824366 35.0 32.0 36.5 19.0 39.0 80-81 31.352998532040345 35.0 32.0 36.0 18.5 38.5 82-83 31.10375320788244 35.0 32.0 36.0 18.0 37.0 84-85 30.8589572356186 35.0 32.0 35.0 17.0 37.0 86-87 30.670191499359298 35.0 32.0 35.0 16.0 36.5 88-89 30.510778251810464 35.0 32.0 35.0 14.0 36.0 90-91 30.38052394150082 35.0 32.0 35.0 11.5 36.0 92-93 30.25277384909748 35.0 31.0 35.0 10.0 36.0 94-95 30.1334266305837 35.0 31.0 35.0 7.0 35.0 96-97 30.077254145907496 34.0 31.0 35.0 7.0 35.0 98-99 29.991130154036654 34.0 31.0 35.0 7.0 35.0 100-101 29.91029618038997 34.0 31.0 35.0 3.5 35.0 102-103 28.905016364427105 33.5 29.5 34.5 2.0 35.0 104-105 29.645924692154054 34.0 31.0 35.0 2.0 35.0 106-107 29.79331093111083 34.0 31.0 35.0 2.0 35.0 108-109 29.759502969149526 34.0 31.0 35.0 2.0 35.0 110-111 29.71599236521064 34.0 31.0 35.0 2.0 35.0 112-113 29.637902799850288 34.0 31.0 35.0 2.0 35.0 114-115 29.61550767035153 34.0 31.0 35.0 2.0 35.0 116-117 29.521204671353097 34.0 31.0 35.0 2.0 35.0 118-119 29.396247666597098 34.0 31.0 35.0 2.0 35.0 120-121 29.208811139236964 34.0 31.0 35.0 2.0 35.0 122-123 28.99077832176883 34.0 31.0 35.0 2.0 35.0 124-125 26.921836978358378 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12901212198577028 1101 2 0.5854455998224104 1101 3 0.30892778064109905 1101 4 0.18133500388844226 1101 5 0.44896880215325297 1101 6 0.2877485137524829 1101 7 0.35265407119767644 1101 8 0.362779797014511 1101 9 0.31559098344023084 1101 10-11 0.3357532453900234 1101 12-13 0.37371457990184354 1101 14-15 0.40732019738509706 1101 16-17 0.4368700055911674 1101 18-19 0.2692757422473093 1101 20-21 0.34174871949179675 1101 22-23 0.21678266890526032 1101 24-25 0.1842179559160826 1101 26-27 0.332387617727413 1101 28-29 0.33050135772675304 1101 30-31 0.1995892974863409 1101 32-33 0.14655733696882578 1101 34-35 0.1936256364834108 1101 36-37 0.23322461371984815 1101 38-39 0.26935980785019353 1101 40-41 0.14274507149805515 1101 42-43 0.12034950760320129 1101 44-45 0.18845945297005073 1101 46-47 0.13178671319293045 1101 48-49 0.1927242725642273 1101 50-51 0.19753468756987047 1101 52-53 0.07160258755774151 1101 54-55 -0.004676795553521629 1101 56-57 0.00809901678053393 1101 58-59 0.14761767299135187 1101 60-61 0.2566547336697482 1101 62-63 0.19943691665894647 1101 64-65 0.17561905728140204 1101 66-67 0.1117553188442173 1101 68-69 0.1923561889366141 1101 70-71 0.18283790877151773 1101 72-73 0.10583746485591661 1101 74-75 0.22120889508045138 1101 76-77 0.1325417159983573 1101 78-79 0.19703329321520258 1101 80-81 0.16777008400455884 1101 82-83 0.1408256121917404 1101 84-85 0.11954244408413928 1101 86-87 0.1755241406604071 1101 88-89 0.14458444094712064 1101 90-91 0.22849348154721483 1101 92-93 0.10695788263921457 1101 94-95 0.06921744888322934 1101 96-97 0.06295452681889913 1101 98-99 0.19270381509467782 1101 100-101 0.22015738074196278 1101 102-103 0.1561124477659881 1101 104-105 0.042321205392472194 1101 106-107 0.1459179364016343 1101 108-109 -0.02778518400457486 1101 110-111 -0.012064489971358228 1101 112-113 0.03697332982262935 1101 114-115 0.05710133511552229 1101 116-117 0.01874077416946207 1101 118-119 0.02889171688891423 1101 120-121 0.08795269317145227 1101 122-123 0.13122488478955674 1101 124-125 0.12609617517075833 1102 1 0.1846861146597618 1102 2 0.5524639148407289 1102 3 0.34188382459714717 1102 4 0.2037415972950356 1102 5 0.3840054321898876 1102 6 0.26108917309313995 1102 7 0.3333353898789966 1102 8 0.3113292475639611 1102 9 0.28596460981385974 1102 10-11 0.255727604364381 1102 12-13 0.3401101842974441 1102 14-15 0.26270114976605186 1102 16-17 0.30987733159849284 1102 18-19 0.3384332514048225 1102 20-21 0.25860219934527606 1102 22-23 0.2562680168906084 1102 24-25 0.2707490914472217 1102 26-27 0.38085465069444524 1102 28-29 0.34197205369745376 1102 30-31 0.15151054290758736 1102 32-33 0.17502253843403537 1102 34-35 0.2281054899632622 1102 36-37 0.25127223276746946 1102 38-39 0.2613451558355493 1102 40-41 0.23215532790830906 1102 42-43 0.240007016760714 1102 44-45 0.32136421487481215 1102 46-47 0.27163653004274835 1102 48-49 0.18708874142869547 1102 50-51 0.21093944947463683 1102 52-53 0.20074361319876033 1102 54-55 0.1451345597578353 1102 56-57 0.14792685560837526 1102 58-59 0.2645663909400682 1102 60-61 0.2836602281752505 1102 62-63 0.1835523012743394 1102 64-65 0.23539195105429656 1102 66-67 0.11651722360612382 1102 68-69 0.1909550900355086 1102 70-71 0.19870970364330987 1102 72-73 0.2107953403137941 1102 74-75 0.19538105625260727 1102 76-77 0.12045197240861683 1102 78-79 0.14689043607234709 1102 80-81 0.18066935140382867 1102 82-83 0.10800876237489021 1102 84-85 0.022472847014540775 1102 86-87 -0.0015637714275058556 1102 88-89 -0.009589551726872259 1102 90-91 0.05761802367175406 1102 92-93 0.07354579472712786 1102 94-95 0.058924408590190325 1102 96-97 -0.005734117869742761 1102 98-99 0.03364520703606999 1102 100-101 0.10078924887383423 1102 102-103 0.17318753934108244 1102 104-105 0.061211315282580614 1102 106-107 0.029218302701998766 1102 108-109 0.002189174969782215 1102 110-111 0.08350144409457627 1102 112-113 0.07043853128782729 1102 114-115 -0.09170452569033927 1102 116-117 -0.10129036136167358 1102 118-119 -0.10142513292793609 1102 120-121 -0.0815766108578515 1102 122-123 -0.012016873452200372 1102 124-125 0.11935258542716554 1103 1 0.10954655675807956 1103 2 0.590467214081901 1103 3 0.2939621265876866 1103 4 0.2458517919502654 1103 5 0.44601357031811517 1103 6 0.14126601242009684 1103 7 0.3011748268085057 1103 8 0.3005952763773294 1103 9 0.21155000517292422 1103 10-11 0.2595505450880182 1103 12-13 0.3575471138036619 1103 14-15 0.3941754706722449 1103 16-17 0.4298447790855704 1103 18-19 0.46853552788122954 1103 20-21 0.3763679532218802 1103 22-23 0.32454559304809294 1103 24-25 0.33060304503789695 1103 26-27 0.26107196071314576 1103 28-29 0.24288440385691246 1103 30-31 0.1744455478564504 1103 32-33 0.31976838688888876 1103 34-35 0.34742826572483665 1103 36-37 0.30391360525179323 1103 38-39 0.2530407458498445 1103 40-41 0.1549867366843003 1103 42-43 0.19250190293689684 1103 44-45 0.08727007652662877 1103 46-47 0.09316913754302192 1103 48-49 0.13308742173222754 1103 50-51 -0.04067739865030262 1103 52-53 0.06374284337582736 1103 54-55 -0.052583025197627364 1103 56-57 -0.07190246364820041 1103 58-59 0.0483108097237519 1103 60-61 -0.046952330936157693 1103 62-63 -0.11169635153552093 1103 64-65 0.08307390420507232 1103 66-67 -0.01608147869234955 1103 68-69 -0.006868510536357064 1103 70-71 0.009530334898123272 1103 72-73 -0.046187613606804234 1103 74-75 -0.10061410486033395 1103 76-77 -0.036573431748422536 1103 78-79 -0.17070727645377204 1103 80-81 -0.034304144692072214 1103 82-83 -0.04643707508249406 1103 84-85 -0.02943961311962795 1103 86-87 -0.030338653204697863 1103 88-89 0.04519896691384062 1103 90-91 0.003013822637992547 1103 92-93 0.026971946712169625 1103 94-95 0.014314268922312579 1103 96-97 0.05959217708241482 1103 98-99 0.09796259403706031 1103 100-101 0.08263902342568485 1103 102-103 0.1074574172691598 1103 104-105 0.07729159681782605 1103 106-107 0.004396351750717287 1103 108-109 0.070338520620286 1103 110-111 0.1166388025021412 1103 112-113 -0.013899530957850459 1103 114-115 0.040869322324617485 1103 116-117 0.06841226703379633 1103 118-119 0.08289731290951963 1103 120-121 0.0781609894940658 1103 122-123 0.14323441875059473 1103 124-125 0.337049275188523 1104 1 0.14249853966737192 1104 2 0.3938842841166803 1104 3 0.17221574314869414 1104 4 0.11001828563114202 1104 5 0.2511470357286285 1104 6 0.23305328419828442 1104 7 0.2849296519538882 1104 8 0.22001328560188682 1104 9 0.22582212875223462 1104 10-11 0.21916849693051432 1104 12-13 0.2087430752876145 1104 14-15 0.23373050201089995 1104 16-17 0.3725301728026693 1104 18-19 0.2670308265762955 1104 20-21 0.17467361896238742 1104 22-23 0.18451178916446764 1104 24-25 0.21896800654241133 1104 26-27 0.07174574831439173 1104 28-29 0.24787667233317023 1104 30-31 0.08612262406950322 1104 32-33 0.15226697332720818 1104 34-35 0.1927466189233087 1104 36-37 0.3457951289511314 1104 38-39 0.3738010379251264 1104 40-41 0.27796001638768786 1104 42-43 0.3444631853883777 1104 44-45 0.5517558049820863 1104 46-47 0.5323845377076353 1104 48-49 0.4782180238298821 1104 50-51 0.4895852704957804 1104 52-53 0.3986536044235365 1104 54-55 0.4912169036393479 1104 56-57 0.5911729022782595 1104 58-59 0.6521221136428395 1104 60-61 0.5259285352724348 1104 62-63 0.45284602071773605 1104 64-65 0.40756831524419823 1104 66-67 0.4083104147449319 1104 68-69 0.6033303116548154 1104 70-71 0.5231785515811254 1104 72-73 0.42996459477752325 1104 74-75 0.41144452443792545 1104 76-77 0.43957595385527526 1104 78-79 0.48398515481730797 1104 80-81 0.33199383787385983 1104 82-83 0.3551917706665897 1104 84-85 0.4059786404564001 1104 86-87 0.4761184936560383 1104 88-89 0.5211365715919527 1104 90-91 0.4699325708520412 1104 92-93 0.4207796635285028 1104 94-95 0.39314923353332887 1104 96-97 0.40958628543261 1104 98-99 0.3016966406219126 1104 100-101 0.2749879272169231 1104 102-103 0.33539796574556746 1104 104-105 0.2909425783782673 1104 106-107 0.2324563979400942 1104 108-109 0.2489922337638184 1104 110-111 0.3085639273052365 1104 112-113 0.2798078251505416 1104 114-115 0.2193005424519967 1104 116-117 0.32548282583723775 1104 118-119 0.30557735637165706 1104 120-121 0.2312193203013777 1104 122-123 0.24577069437674126 1104 124-125 0.1551835995928954 1105 1 0.15669256419216993 1105 2 0.514964613540073 1105 3 0.22974881438538475 1105 4 0.08696937328127063 1105 5 0.3536539326736019 1105 6 0.1802558935059153 1105 7 0.28815039579107093 1105 8 0.15251123187010052 1105 9 0.21726720037604252 1105 10-11 0.04747779247574613 1105 12-13 0.1055013484380467 1105 14-15 0.16949100250172933 1105 16-17 0.2338205757132954 1105 18-19 0.1401821496097 1105 20-21 0.147323472053003 1105 22-23 0.18816181459027348 1105 24-25 0.05124377058298535 1105 26-27 0.1279347323885176 1105 28-29 0.10692593954072294 1105 30-31 0.08197469425661552 1105 32-33 -0.007719157792990927 1105 34-35 0.03418331432099109 1105 36-37 0.017705426360926424 1105 38-39 0.012779961812114493 1105 40-41 -0.039087711971298233 1105 42-43 -0.08698900602762905 1105 44-45 0.16656705266907323 1105 46-47 0.138568272491419 1105 48-49 0.056967920188235155 1105 50-51 0.0776607221823582 1105 52-53 1.837937856734584E-4 1105 54-55 0.09289700198077355 1105 56-57 0.16309207113025082 1105 58-59 0.16284630064661343 1105 60-61 0.11852962514396381 1105 62-63 0.1792723915677854 1105 64-65 0.07913181991380469 1105 66-67 0.10234222629343037 1105 68-69 0.10412718505862273 1105 70-71 0.27062542975316717 1105 72-73 0.1829155166010139 1105 74-75 0.1441917624764102 1105 76-77 0.22150840581718967 1105 78-79 0.14767663407299025 1105 80-81 0.1629456063753416 1105 82-83 0.1475018291275525 1105 84-85 0.2215387397373192 1105 86-87 0.1187576302708706 1105 88-89 0.24424598519670226 1105 90-91 0.09007680041043997 1105 92-93 0.19172100702423833 1105 94-95 0.05831277793341272 1105 96-97 0.06326534466921885 1105 98-99 0.07155749339530715 1105 100-101 0.13039367755275322 1105 102-103 0.08585936563367724 1105 104-105 0.024924956043623325 1105 106-107 0.10397633774445936 1105 108-109 0.12521099588776607 1105 110-111 0.18140298123313414 1105 112-113 0.24929954386441722 1105 114-115 0.16280197758817394 1105 116-117 0.1781276758306305 1105 118-119 0.13107771561554316 1105 120-121 0.21256764368369474 1105 122-123 0.15687488189553633 1105 124-125 0.14617576074695648 1106 1 0.16242730379078196 1106 2 0.5160409931296712 1106 3 0.2018547689070722 1106 4 0.07835876167490596 1106 5 0.2710541273741711 1106 6 0.11917687823169132 1106 7 0.2450964875383974 1106 8 0.16928566402884826 1106 9 0.1305467997519827 1106 10-11 0.24292911095572123 1106 12-13 0.1657426836248561 1106 14-15 -0.05195475553392015 1106 16-17 0.09823245669937819 1106 18-19 0.10840365764641291 1106 20-21 0.12126994214860787 1106 22-23 0.04093629776227914 1106 24-25 0.15371600024464271 1106 26-27 0.18281967625269147 1106 28-29 0.16460428399069826 1106 30-31 0.14540669571899656 1106 32-33 0.003878067086176884 1106 34-35 -0.01830613231276601 1106 36-37 0.026680357599317972 1106 38-39 0.11452567776183287 1106 40-41 -0.007859738005059569 1106 42-43 -0.02096043010503479 1106 44-45 -0.12972322122957536 1106 46-47 -0.08703336503392478 1106 48-49 -0.010757909095922003 1106 50-51 -0.16807265706289343 1106 52-53 -0.05540704593494894 1106 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52-53 -0.4978161814576296 2216 54-55 -0.6885551913963894 2216 56-57 -1.2523370242522844 2216 58-59 -1.2717378909406918 2216 60-61 -0.8594309263824869 2216 62-63 -0.8102187872800926 2216 64-65 -0.6455039604079431 2216 66-67 -0.6770371207777615 2216 68-69 -0.5382103682374009 2216 70-71 -0.3798080889129807 2216 72-73 -0.5003899522841699 2216 74-75 -0.5469549938832294 2216 76-77 -0.3927791558144591 2216 78-79 -0.531837900344474 2216 80-81 -0.400109954228121 2216 82-83 -0.35345604419876864 2216 84-85 -0.4885720385630705 2216 86-87 -0.6048039334356083 2216 88-89 -0.6431667639208136 2216 90-91 -0.538112829537571 2216 92-93 -0.4919238787565483 2216 94-95 -0.5814502768107452 2216 96-97 -0.6309562456427997 2216 98-99 -0.5883800275589941 2216 100-101 -0.7177876799726093 2216 102-103 -0.6941180926071802 2216 104-105 -0.5278119358799778 2216 106-107 -0.4439524225009279 2216 108-109 -0.5052657311088531 2216 110-111 -0.5670848852218882 2216 112-113 -0.5756278386973364 2216 114-115 -0.5905382007074209 2216 116-117 -0.5806572630400915 2216 118-119 -0.6794177658929215 2216 120-121 -0.5800936033741451 2216 122-123 -0.6389182185194535 2216 124-125 -0.722771787458143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76.0 3 75.0 4 211.0 5 495.0 6 1190.0 7 2312.0 8 3655.0 9 6467.0 10 11081.0 11 14108.0 12 14056.0 13 17524.0 14 24490.0 15 18435.0 16 8419.0 17 7091.0 18 6828.0 19 6377.0 20 6349.0 21 6456.0 22 7123.0 23 8434.0 24 10526.0 25 13351.0 26 16738.0 27 21105.0 28 26942.0 29 33827.0 30 41983.0 31 52701.0 32 66567.0 33 85258.0 34 114625.0 35 165370.0 36 278851.0 37 440564.0 38 175101.0 39 545.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.487968992284245 17.7250702095611 10.752067783874727 22.03489301427993 2 19.07016685740137 21.384168455958356 36.93103876471538 22.61462592192489 3 17.54167191355002 28.411115464081004 31.648733985346038 22.398478637022944 4 11.260737886265124 16.26218077834554 36.905040211507576 35.57204112388176 5 10.45988854298423 41.612486172889064 35.162693171157464 12.764932112969246 6 29.729231827575852 37.65260405583847 18.246958995630514 14.371205120955166 7 26.823901974341602 34.17075437268919 22.7339028721406 16.271440780828613 8 28.323809279510474 32.29703621394667 20.367328045258397 19.01182646128446 9 27.852115016212853 11.349986533015102 19.914172748186036 40.88372570258601 10-11 24.30417663087519 25.74601849465926 29.623985457988255 20.32581941647729 12-13 25.785282625957116 25.231999421677532 29.48013357383464 19.50258437853071 14-15 22.892475161866162 26.01865206557897 27.909568321920403 23.179304450634465 16-17 21.37729984542112 28.22118275724062 28.436333935849913 21.965183461488348 18-19 20.580211513342668 25.65303964803937 32.37018128840606 21.396567550211902 20-21 21.70327043687832 25.201625832358776 32.64108561387881 20.4540181168841 22-23 21.444978330397024 25.456274274094536 32.330441332333706 20.768306063174734 24-25 21.153426852118514 25.612689514290743 32.53643956238712 20.697444071203623 26-27 20.92682005426437 25.662330802783877 32.532562703097874 20.87828643985388 28-29 20.88984783751223 25.085341990299682 32.948173341817736 21.07663683037035 30-31 20.790517843463498 25.343495562890823 32.843498477822614 21.022488115823066 32-33 20.65319540653388 25.57829331909292 32.57031106986158 21.198200204511615 34-35 21.107670190048054 25.56947664640008 32.303797055097554 21.019056108454308 36-37 21.496397727285977 25.101031536064117 31.513852338882977 21.88871839776693 38-39 21.212570818267995 25.54579182956277 31.75395527095457 21.48768208121466 40-41 21.216657907294064 25.565037831453346 31.76536191366494 21.452942347587644 42-43 21.40883679957371 25.633417460570097 31.14418872018745 21.81355701966875 44-45 21.270792562531103 25.88727420397271 30.839357913250133 22.002575320246056 46-47 21.771786567003186 26.081930843884194 29.912396034062056 22.233886555050567 48-49 21.8783470280286 26.40756649161789 29.154745878035683 22.559340602317825 50-51 22.076126020023835 26.34264754926082 29.054995580576925 22.52623085013841 52-53 22.060726941567854 26.43077776987452 28.96076577117584 22.547729517381786 54-55 21.963271915798558 26.704456840346964 28.95860328917354 22.373667954680943 56-57 22.23588437619975 26.70673195349465 28.597589611243702 22.4597940590619 58-59 22.224457099680976 25.99855296542975 29.035255872091938 22.741734062797335 60-61 22.239874165289816 26.31078794775854 29.26735561714626 22.181982269805385 62-63 22.061308554403393 26.510013411847495 29.23741943344928 22.191258600299836 64-65 22.042830054204373 26.28097366737258 29.42867511918695 22.247521159236097 66-67 22.05575847322993 26.737773683796284 28.997904101722483 22.2085637412513 68-69 22.293318362042882 26.440622334623498 29.04756824384081 22.218491059492806 70-71 22.19413759781723 26.33536461832879 29.1011964710735 22.36930131278048 72-73 22.246607878740356 26.568281279559997 28.5766630918959 22.608447749803748 74-75 21.98365873992153 26.748538148068256 28.772831416261972 22.49497169574824 76-77 21.648392778126603 26.735844513665047 29.130193221084028 22.485569487124327 78-79 21.869965361835174 26.635270880476327 28.834617993481626 22.66014576420688 80-81 21.608692623157495 26.953842010128298 28.470696122850114 22.966769243864096 82-83 21.945556006398867 26.611667669404376 28.39665738551301 23.046118938683755 84-85 21.40190596341574 26.42324187511168 28.761607659998933 23.41324450147365 86-87 21.558923546273856 26.627369339106476 28.572219770430696 23.24148734418897 88-89 21.786879133993345 26.36599776537355 28.607153777920807 23.239969322712298 90-91 21.689129214916083 26.65836861704841 28.53350434700228 23.118997821033226 92-93 21.59047416666594 26.58757816082204 28.432335340861492 23.38961233165053 94-95 21.310383486277576 26.17487617885513 28.786962335227003 23.72777799964029 96-97 21.851593496878184 26.33226226681129 28.498920139908122 23.3172240964024 98-99 21.620150734067035 26.35307217949335 28.226914382408623 23.799862704030993 100-101 21.455967958677423 26.246134024374957 28.463549789102103 23.834348227845517 102-103 21.655572306892264 25.827850282693383 28.3072910290179 24.209286381396456 104-105 21.284060507446835 26.175411431166573 28.50049525066563 24.04003281072097 106-107 21.38666267123188 26.052867997325237 28.494057276578726 24.066412054864163 108-109 21.598399565786316 26.230663193190402 27.76347014154212 24.407467099481163 110-111 21.459388854281777 26.299046835616092 27.877820993752355 24.363743316349776 112-113 21.367883659397517 25.91184081180011 28.22094545980356 24.499330068998812 114-115 21.078837835536913 26.23161886902034 27.88853008097086 24.801013214471894 116-117 20.95121197455349 26.230633772019335 28.11955894272203 24.698595310705144 118-119 20.943538470418872 26.03957677029346 27.922385730952826 25.094499028334837 120-121 20.792157137110173 26.095447151627514 28.01817874404585 25.09421696721646 122-123 20.988443208942257 26.189290699179445 27.631703224497457 25.19056286738084 124-125 20.829493893578164 26.027626475591077 27.511532894803963 25.631346736026796 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 150.5 2 562.0 3 1025.0 4 824.0 5 754.0 6 1123.0 7 2434.0 8 3830.0 9 4489.0 10 4019.5 11 2970.0 12 2767.0 13 2872.5 14 3027.5 15 3235.5 16 3459.5 17 3601.5 18 3797.5 19 4027.5 20 4287.0 21 4710.5 22 5185.5 23 6257.5 24 7588.5 25 8651.0 26 10296.5 27 11913.0 28 13113.5 29 15548.0 30 19990.0 31 23983.0 32 28538.0 33 33635.5 34 39057.0 35 43610.5 36 48335.5 37 53181.0 38 54096.0 39 54892.5 40 54049.5 41 53246.5 42 53113.5 43 54389.0 44 58381.5 45 60975.5 46 60435.0 47 58593.0 48 58018.5 49 56646.0 50 56270.0 51 53278.5 52 49730.5 53 49698.0 54 48894.0 55 46008.0 56 42454.5 57 39072.5 58 36922.5 59 35137.5 60 31250.5 61 26868.5 62 22476.5 63 17338.5 64 13092.5 65 10528.5 66 7982.5 67 5945.5 68 4431.0 69 2994.5 70 2262.0 71 1936.0 72 1698.5 73 1601.5 74 1282.0 75 890.0 76 576.5 77 392.0 78 251.5 79 140.0 80 80.0 81 22.0 82 12.0 83 7.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06716002858965106 2 5.246877233566489E-4 3 1.1659727185703308E-4 4 5.829863592851654E-5 5 0.0747388512603582 6 5.829863592851654E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.914931796425827E-5 18-19 2.914931796425827E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 8.744795389277482E-5 30-31 0.0 32-33 0.0 34-35 1.7489590778554964E-4 36-37 0.0 38-39 0.0 40-41 2.914931796425827E-5 42-43 0.0027400358886402777 44-45 0.01046460514916872 46-47 0.011718025821631825 48-49 0.00961927492820523 50-51 0.008744795389277481 52-53 0.0069958363114219855 54-55 0.007782867896456959 56-57 0.006150506090458495 58-59 0.004314099058710224 60-61 0.0023319454371406615 62-63 0.001836407031748271 64-65 0.002244497483247887 66-67 0.002973230432354344 68-69 0.0013408686263558806 70-71 0.0031189770221756352 72-73 0.002594289298818986 74-75 9.327781748562647E-4 76-77 9.910768107847811E-4 78-79 8.744795389277482E-5 80-81 7.287329491064568E-4 82-83 1.1659727185703308E-4 84-85 3.20642497606841E-4 86-87 4.0809045149961586E-4 88-89 0.0017781083958197546 90-91 0.0025359906628904696 92-93 7.287329491064568E-4 94-95 0.0017198097598912381 96-97 0.0021861988473193704 98-99 0.001953004303605304 100-101 0.0012242713544988475 102-103 0.0011659727185703308 104-105 7.578822670707151E-4 106-107 8.744795389277482E-4 108-109 0.0012534206724631057 110-111 0.0011368234006060725 112-113 0.011892921729417376 114-115 0.02145389802169409 116-117 0.01958834167198156 118-119 0.012184414909059957 120-121 0.00676264176770792 122-123 0.007229030855136051 124-125 0.006033908818601462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1715306.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.197226323123136 #Duplication Level Percentage of deduplicated Percentage of total 1 42.0576229832275 9.33562575971286 2 14.756394983142204 6.551020783084127 3 11.082605270060892 7.380092922881365 4 8.34208578933595 7.406846650911976 5 6.033958066655196 6.6968566414889965 6 4.19967422003927 5.593267148735839 7 2.917711076303736 4.533556517433805 8 2.042797445481736 3.6275549783724714 9 1.4828693567918432 2.962402804829932 >10 6.355017474006646 24.42766879352356 >50 0.45572909355240016 7.0287987070644 >100 0.25887114156148544 10.352654614034195 >500 0.010930674427108626 1.5934324372959334 >1k 0.0029326199682486558 0.9774464668653692 >5k 5.332036305906646E-4 0.91382989291851 >10k+ 2.666018152953323E-4 0.6189448808466972 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10459 0.6097454331763545 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7800 0.454729360242429 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7642 0.44551817576572345 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.15437478793871182 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2009 0.11712195958038973 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.829863592851654E-5 0.0 16-17 0.0 0.0 0.0 5.829863592851654E-5 0.0 18-19 0.0 0.0 0.0 1.1659727185703308E-4 0.0 20-21 0.0 0.0 0.0 2.6234386167832446E-4 0.0 22-23 0.0 0.0 0.0 4.080904514996158E-4 0.0 24-25 0.0 0.0 0.0 5.246877233566489E-4 0.0 26-27 0.0 0.0 0.0 6.704343131779403E-4 0.0 28-29 0.0 0.0 0.0 0.0012242713544988475 0.0 30-31 0.0 0.0 0.0 0.0034396195197824763 0.0 32-33 0.0 0.0 0.0 0.009590125610240972 0.0 34-35 0.0 0.0 0.0 0.017985129183947353 0.0 36-37 0.0 0.0 0.0 0.03145211408343467 0.0 38-39 0.0 0.0 0.0 0.05343069982848541 0.0 40-41 0.0 0.0 0.0 0.07794527623642661 0.0 42-43 0.0 0.0 0.0 0.10782332714979134 0.0 44-45 0.0 0.0 0.0 0.14239441825540167 0.0 46-47 0.0 0.0 0.0 0.19017015039882096 0.0 48-49 0.0 0.0 0.0 0.2390535566248821 0.0 50-51 0.0 0.0 0.0 0.28866569580004964 0.0 52-53 0.0 0.0 0.0 0.3448947301531039 0.0 54-55 0.0 0.0 0.0 0.4103349489828637 0.0 56-57 0.0 0.0 0.0 0.49693757265467503 0.0 58-59 0.0 0.0 0.0 0.6000678596122209 0.0 60-61 0.0 0.0 0.0 0.69952533250627 0.0 62-63 0.0 0.0 0.0 0.7977002354098919 0.0 64-65 0.0 0.0 0.0 0.9086133902638946 0.0 66-67 0.0 0.0 0.0 1.0529899621408658 0.0 68-69 0.0 0.0 0.0 1.2395455971121188 0.0 70-71 0.0 0.0 0.0 1.4170066448785232 0.0 72-73 0.0 0.0 0.0 1.5853148068041505 0.0 74-75 0.0 0.0 0.0 1.7694218990664057 0.0 76-77 0.0 0.0 0.0 1.9877794399366644 0.0 78-79 0.0 0.0 0.0 2.2711108105492546 0.0 80-81 0.0 0.0 0.0 2.560505239298411 0.0 82-83 0.0 0.0 0.0 2.8506284009966736 0.0 84-85 0.0 0.0 0.0 3.1369038527236537 0.0 86-87 0.0 0.0 0.0 3.4473149397250404 0.0 88-89 0.0 0.0 0.0 3.8382947415796362 0.0 90-91 0.0 0.0 0.0 4.2592400423014904 0.0 92-93 0.0 0.0 0.0 4.675783796010741 0.0 94-95 0.0 0.0 0.0 5.110808217309332 0.0 96-97 0.0 0.0 0.0 5.570697006831434 0.0 98-99 0.0 5.829863592851654E-5 0.0 6.086173545711377 0.0 100-101 0.0 5.829863592851654E-5 0.0 6.660706602786908 0.0 102-103 0.0 5.829863592851654E-5 0.0 7.227194448104303 0.0 104-105 0.0 5.829863592851654E-5 0.0 7.796422329310339 0.0 106-107 0.0 5.829863592851654E-5 0.0 8.378388462466756 0.0 108-109 0.0 5.829863592851654E-5 0.0 9.010782915701338 0.0 110-111 0.0 5.829863592851654E-5 0.0 9.711649116833964 0.0 112-113 0.0 5.829863592851654E-5 0.0 10.401059636006636 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6400 0.0 73.55029 1 GTATCAA 9855 0.0 47.78757 2 GTACATG 14305 0.0 45.318794 1 ACATGGG 14860 0.0 42.99085 3 TACATGG 14980 0.0 42.924675 2 TACCTGG 1370 0.0 41.720142 2 ATGGGAT 3925 0.0 40.652775 5 CATGGGA 10760 0.0 39.9503 4 TATCACG 185 0.0 38.61932 2 TATAACG 220 0.0 37.887894 2 ATCACGC 190 0.0 37.60302 3 CATGGGG 3975 0.0 36.247105 4 ATGGGAG 3475 0.0 35.808537 5 TCAACGC 13725 0.0 35.787937 5 ATCAACG 13845 0.0 35.47775 4 CAACGCA 14045 0.0 35.0277 6 AACGCAG 14280 0.0 34.45126 7 TATCAAC 14610 0.0 33.823845 3 TAACGCA 250 0.0 33.341347 4 ATATAGG 775 0.0 33.034054 2 >>END_MODULE