FastQCFastQC Report
Thu 26 May 2016
SRR936203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987449
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.065.763241
GTACATG156150.056.694031
TACATGG159050.054.9872
ACATGGG161550.051.6686443
CATGGGA106150.045.678734
CATGGGG58900.045.3531464
GTATCAA36600.045.0272142
ATGGGAG39400.041.5253075
ATGGGAT41500.037.9904255
TCAACGC45150.036.1051525
ATCAACG46150.035.0649764
AACGCAG46600.034.854047
CAACGCA46900.034.504246
ATGGGGA35100.034.2389645
GAGTACA89000.033.824931
AGTACAT91700.031.6611632
ATGGGGG30550.031.3538635
TATCAAC53750.031.1031383
ATGGGAC21700.029.6101365
TACCTGG18250.028.6877292
Produced by FastQC (version 0.11.5)