FastQCFastQC Report
Thu 26 May 2016
SRR936203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987449
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65840.3312789409942092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49710.2501196257111503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49480.24896236331095795No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21420.1077763504874842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.083.8008961
GTATCAA73000.052.681952
GTACATG130350.049.4102331
TACATGG135650.047.1781462
ACATGGG134950.046.2758943
CATGGGA86900.042.748124
ATCAACG106750.037.810644
TCAACGC106950.037.6286055
ATGGGAT33550.037.6179855
CAACGCA108450.037.3023766
AACGCAG110800.036.672297
TATCAAC113950.035.3693053
ATGGGAG35350.035.3656855
CATGGGG53150.034.8344764
GTACCGT1209.303003E-734.703556
GAGTACA87150.031.7214341
ACGCAGA130150.030.900088
AGTACAT85350.030.8298152
CGCAGAG133000.030.1932059
TATAACG1653.393743E-728.8641152
Produced by FastQC (version 0.11.5)