##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936203_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1987449 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.503991800544316 31.0 31.0 34.0 30.0 34.0 2 31.37714829411975 31.0 31.0 34.0 30.0 34.0 3 31.69129773896085 31.0 31.0 34.0 30.0 34.0 4 35.56718486864317 37.0 35.0 37.0 33.0 37.0 5 35.16072211161142 37.0 35.0 37.0 33.0 37.0 6 35.35041050109965 37.0 35.0 37.0 33.0 37.0 7 35.19284821899832 37.0 35.0 37.0 32.0 37.0 8 35.27044870082201 37.0 35.0 37.0 32.0 37.0 9 36.95698053132433 39.0 37.0 39.0 33.0 39.0 10-11 36.833817119332366 39.0 37.0 39.0 32.0 39.0 12-13 36.76794624667098 39.0 37.0 39.0 32.0 39.0 14-15 37.93277563348795 40.0 37.0 41.0 32.5 41.0 16-17 37.8416759373448 40.0 37.0 41.0 32.0 41.0 18-19 37.9351283982633 40.0 37.0 41.0 32.0 41.0 20-21 37.98786182689468 40.0 37.0 41.0 32.5 41.0 22-23 37.89562071781465 40.0 37.0 41.0 32.0 41.0 24-25 37.85721771980061 40.0 37.0 41.0 32.0 41.0 26-27 37.675871431166286 40.0 37.0 41.0 32.0 41.0 28-29 37.51405520342912 40.0 36.5 41.0 32.0 41.0 30-31 37.33644913655646 39.5 36.0 41.0 31.5 41.0 32-33 37.12975376978227 39.0 36.0 41.0 31.0 41.0 34-35 36.92619181674599 39.0 35.0 41.0 30.5 41.0 36-37 36.716765310707345 39.0 35.0 41.0 30.0 41.0 38-39 36.695210543767416 39.0 35.0 41.0 30.0 41.0 40-41 36.70459241973002 39.0 35.0 41.0 30.0 41.0 42-43 36.57740173458539 39.0 35.0 41.0 30.0 41.0 44-45 36.39950886790051 39.0 35.0 41.0 30.0 41.0 46-47 36.089716012838565 39.0 35.0 41.0 29.5 41.0 48-49 35.78839356380969 39.0 35.0 41.0 28.0 41.0 50-51 35.57584722928739 39.0 35.0 41.0 27.0 41.0 52-53 35.39781222059031 39.0 35.0 41.0 27.0 41.0 54-55 35.18826118305425 38.5 34.5 40.5 26.5 41.0 56-57 34.958188361059825 38.0 34.0 40.0 26.0 41.0 58-59 34.71263564499014 38.0 34.0 40.0 26.0 41.0 60-61 34.46525420274935 37.0 34.0 40.0 26.0 41.0 62-63 34.22614391614577 37.0 34.0 40.0 26.0 41.0 64-65 33.959436694979345 36.5 33.0 40.0 25.5 41.0 66-67 33.66363363286303 36.0 33.0 39.0 25.0 41.0 68-69 33.34974457206198 35.5 33.0 39.0 24.5 41.0 70-71 33.01703288990057 35.0 33.0 38.5 24.0 40.0 72-73 32.76546618303162 35.0 32.5 37.5 24.0 40.0 74-75 32.6036811510635 35.0 33.0 37.0 24.5 39.5 76-77 32.32039891338093 35.0 32.5 37.0 24.0 39.0 78-79 32.017359690739234 35.0 32.0 36.0 24.0 39.0 80-81 31.767092639861453 35.0 32.0 36.0 23.0 37.5 82-83 31.54356992305211 35.0 32.0 35.5 23.0 37.0 84-85 31.328173704079955 35.0 32.0 35.0 22.5 37.0 86-87 31.146563760881413 35.0 32.0 35.0 22.0 36.0 88-89 31.01509648801051 35.0 32.0 35.0 21.5 36.0 90-91 30.898075371996967 35.0 32.0 35.0 20.0 36.0 92-93 30.782238688892143 35.0 32.0 35.0 20.0 35.5 94-95 30.683949877455973 34.0 32.0 35.0 20.0 35.0 96-97 30.63073568177095 34.0 32.0 35.0 20.0 35.0 98-99 30.545500035472607 34.0 31.5 35.0 19.0 35.0 100-101 30.463074775755253 34.0 31.0 35.0 19.0 35.0 102-103 29.430239216201272 33.5 29.5 34.5 17.5 35.0 104-105 30.206929083463272 34.0 31.0 35.0 18.0 35.0 106-107 30.366908534508305 34.0 31.0 35.0 18.0 35.0 108-109 30.33463550511233 34.0 31.0 35.0 18.0 35.0 110-111 30.297233790653245 34.0 31.0 35.0 18.0 35.0 112-113 30.220438109355257 34.0 31.0 35.0 17.0 35.0 114-115 30.20126227138407 34.0 31.5 35.0 15.0 35.0 116-117 30.1017772028364 34.0 31.0 35.0 8.5 35.0 118-119 29.96112403387458 34.0 31.0 35.0 2.0 35.0 120-121 29.76313933087088 34.0 31.0 35.0 2.0 35.0 122-123 29.523889418042927 34.0 31.0 35.0 2.0 35.0 124-125 27.387666048286018 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1717059417892024 1101 2 0.5584252565717982 1101 3 0.3785846871311165 1101 4 0.2282446796089559 1101 5 0.4797228994638161 1101 6 0.2598648361997178 1101 7 0.31990488760219193 1101 8 0.3616635780714148 1101 9 0.16880523546327453 1101 10-11 0.1667069866177826 1101 12-13 0.2957371605411723 1101 14-15 0.3559009688220698 1101 16-17 0.4479405952076121 1101 18-19 0.3747448346393085 1101 20-21 0.27194008871041575 1101 22-23 0.26148152979303774 1101 24-25 0.26864046089043114 1101 26-27 0.3361473832027926 1101 28-29 0.3288428554206604 1101 30-31 0.18735840919097058 1101 32-33 0.20248452032468833 1101 34-35 0.19765492741797175 1101 36-37 0.2481364677695268 1101 38-39 0.2823340707937021 1101 40-41 0.20994115247124512 1101 42-43 0.19727535635710325 1101 44-45 0.1862614873316346 1101 46-47 0.2505210546226877 1101 48-49 0.2509691196592385 1101 50-51 0.1108397741295235 1101 52-53 0.15140647487831416 1101 54-55 0.12663013102656606 1101 56-57 -0.16497825835170232 1101 58-59 0.0572550930044784 1101 60-61 0.1169025141704978 1101 62-63 0.07441124949252753 1101 64-65 -0.003170095670711248 1101 66-67 -0.001611150673952011 1101 68-69 -0.06006859045981372 1101 70-71 0.028287929867197192 1101 72-73 -0.05391739442004706 1101 74-75 0.02893282080983539 1101 76-77 0.10464833963383313 1101 78-79 0.06794623860852411 1101 80-81 0.06748281187894634 1101 82-83 0.12414085564529742 1101 84-85 0.013638211687013069 1101 86-87 0.010209753425204582 1101 88-89 0.04249144136885974 1101 90-91 0.13956505457251822 1101 92-93 0.19402951239108646 1101 94-95 0.15847956008440534 1101 96-97 0.14496550714905965 1101 98-99 0.18532607837223836 1101 100-101 0.2045524760590567 1101 102-103 0.1923321461497629 1101 104-105 0.16757787367139443 1101 106-107 0.128802471886587 1101 108-109 0.06775299416554859 1101 110-111 0.12786294130852482 1101 112-113 0.14827008620195414 1101 114-115 -0.05381853814100879 1101 116-117 -0.05453668579540505 1101 118-119 0.10184282763668051 1101 120-121 0.20374181933728508 1101 122-123 0.14584916048374197 1101 124-125 0.08883974584601972 1102 1 0.22739906400430243 1102 2 0.6084918476042063 1102 3 0.2889515132874152 1102 4 0.1344713521948293 1102 5 0.38105307588894277 1102 6 0.2870142467457626 1102 7 0.38267474570574933 1102 8 0.2789262755837001 1102 9 0.3328954046538257 1102 10-11 0.4045158950181218 1102 12-13 0.4213788185756613 1102 14-15 0.4510110495534292 1102 16-17 0.4170719521848696 1102 18-19 0.382513788420205 1102 20-21 0.35128864754893385 1102 22-23 0.4168162113892464 1102 24-25 0.36281004605296374 1102 26-27 0.36226093324471975 1102 28-29 0.3568406850462722 1102 30-31 0.3206802329631486 1102 32-33 0.3554202805311988 1102 34-35 0.42805003421025845 1102 36-37 0.3918925308419148 1102 38-39 0.35450559613940413 1102 40-41 0.16164210118793676 1102 42-43 0.2526659415360868 1102 44-45 0.3436761110511952 1102 46-47 0.42456206154486154 1102 48-49 0.30386213006718066 1102 50-51 0.3438903011030021 1102 52-53 0.4643974809783771 1102 54-55 0.4023400243164943 1102 56-57 0.36877435185234475 1102 58-59 0.2691099409960671 1102 60-61 0.3495304758272155 1102 62-63 0.3927295381852076 1102 64-65 0.3927144141041907 1102 66-67 0.3379751628136134 1102 68-69 0.22482922107933945 1102 70-71 0.29617296579346686 1102 72-73 0.2586609117013907 1102 74-75 0.272595163169953 1102 76-77 0.2609728434895402 1102 78-79 0.2542219090404636 1102 80-81 0.2612331201707647 1102 82-83 0.3041092865632713 1102 84-85 0.33143857012763434 1102 86-87 0.3138714380961183 1102 88-89 0.3130746472749095 1102 90-91 0.30895291034127226 1102 92-93 0.40744966070000643 1102 94-95 0.37053664750774473 1102 96-97 0.33837217282918886 1102 98-99 0.32723074632140126 1102 100-101 0.34618091454045086 1102 102-103 0.4259597954041112 1102 104-105 0.3013600798898466 1102 106-107 0.28207200936664734 1102 108-109 0.25040479705609187 1102 110-111 0.2599351555836691 1102 112-113 0.3308166588190673 1102 114-115 0.31986896083135363 1102 116-117 0.38968962506139704 1102 118-119 0.3703099185128913 1102 120-121 0.4184066444741674 1102 122-123 0.22778572635953864 1102 124-125 0.3131347852251629 1103 1 0.15093450099208994 1103 2 0.6473560091739259 1103 3 0.34427830032151974 1103 4 0.20911592646223198 1103 5 0.3749987831864203 1103 6 0.20854029424616272 1103 7 0.34713815925137936 1103 8 0.3519663860892379 1103 9 0.300708163515111 1103 10-11 0.29206083687535056 1103 12-13 0.36449219669030697 1103 14-15 0.4081602200543699 1103 16-17 0.5249324095550136 1103 18-19 0.4303216925268245 1103 20-21 0.4068777654966098 1103 22-23 0.4036762627451509 1103 24-25 0.29592763670135724 1103 26-27 0.2598713272091473 1103 28-29 0.38866980639009796 1103 30-31 0.3027383223885991 1103 32-33 0.29281789255792745 1103 34-35 0.3843294852093493 1103 36-37 0.18796997615245203 1103 38-39 0.31506778979186123 1103 40-41 0.2308887542917688 1103 42-43 0.2721676869618861 1103 44-45 0.3236159599877695 1103 46-47 0.3544282727789465 1103 48-49 0.33399208727784213 1103 50-51 0.3875259463227607 1103 52-53 0.26347322712477705 1103 54-55 0.32064843621303396 1103 56-57 0.20373065361780363 1103 58-59 0.4483592391792328 1103 60-61 0.3349515169435051 1103 62-63 0.2592319486906405 1103 64-65 0.35177936223124817 1103 66-67 0.26868932334924267 1103 68-69 0.2005647000836106 1103 70-71 0.23787509067387447 1103 72-73 0.3125764455012572 1103 74-75 0.31474880200450883 1103 76-77 0.2679779496900139 1103 78-79 0.26151274911983435 1103 80-81 0.2603024441080848 1103 82-83 0.2531746374390309 1103 84-85 0.16117056807688002 1103 86-87 0.19704546798877587 1103 88-89 0.15822756660434578 1103 90-91 0.23070754494478862 1103 92-93 0.2388672164072254 1103 94-95 0.2850548675326259 1103 96-97 0.21455633566158028 1103 98-99 0.21887447263950222 1103 100-101 0.2744872909232683 1103 102-103 0.2084700518897229 1103 104-105 0.20783094537504354 1103 106-107 0.2729388660871628 1103 108-109 0.22836627904070994 1103 110-111 0.26356805775818515 1103 112-113 0.252201200017538 1103 114-115 0.2786356572143447 1103 116-117 0.2715607084663034 1103 118-119 0.30191284747563785 1103 120-121 0.257763792229607 1103 122-123 0.29639070280962443 1103 124-125 0.28596448674482744 1104 1 0.1644655086906468 1104 2 0.36239367564319025 1104 3 0.24844709017429878 1104 4 0.08719293958696994 1104 5 0.4475490854822155 1104 6 0.2597563634367859 1104 7 0.33928535457991416 1104 8 0.2732785839655918 1104 9 0.30545813252807363 1104 10-11 0.3017800942881479 1104 12-13 0.260602634502753 1104 14-15 0.2677799746370084 1104 16-17 0.4218729557532441 1104 18-19 0.3906507060371709 1104 20-21 0.36564916376960355 1104 22-23 0.35528793315322105 1104 24-25 0.21938689218606555 1104 26-27 0.21526513806426095 1104 28-29 0.3147020191402419 1104 30-31 0.2766599733781234 1104 32-33 0.2384036164840495 1104 34-35 0.2806120732469708 1104 36-37 0.32841770445807583 1104 38-39 0.31158209203256604 1104 40-41 0.11340930902865409 1104 42-43 0.12258566770861279 1104 44-45 0.09315569914575406 1104 46-47 0.05629438121981423 1104 48-49 0.05468592154537788 1104 50-51 -0.07084640772828266 1104 52-53 -0.07661367430888788 1104 54-55 -0.13254770486751966 1104 56-57 -0.039396765092860164 1104 58-59 -0.027812070952840884 1104 60-61 0.07891351994580731 1104 62-63 -0.01495619084744959 1104 64-65 0.02434137074235565 1104 66-67 0.041955770581758145 1104 68-69 0.11448166858701114 1104 70-71 0.011273060678412605 1104 72-73 0.04627726218193118 1104 74-75 0.03625621823621117 1104 76-77 0.06991773842725735 1104 78-79 -0.016354929331090062 1104 80-81 0.021939110728837363 1104 82-83 -0.02031044057945408 1104 84-85 -0.05019478978900693 1104 86-87 3.089719372084687E-5 1104 88-89 0.03677730424740133 1104 90-91 0.020926313450939205 1104 92-93 -0.06588757281203783 1104 94-95 -0.07014338066668202 1104 96-97 0.08875928011903156 1104 98-99 0.10914798491726074 1104 100-101 -0.0019670341789890244 1104 102-103 -0.025658730250725625 1104 104-105 0.005878417521138601 1104 106-107 -0.05613145383681584 1104 108-109 -0.0633625912406579 1104 110-111 -0.00991529875804531 1104 112-113 0.019838334886909337 1104 114-115 -0.04106555885484653 1104 116-117 0.06718981424917203 1104 118-119 0.033530990732167254 1104 120-121 0.03743078483403295 1104 122-123 0.12889645262714566 1104 124-125 0.00999218227305576 1105 1 0.22013311505615363 1105 2 0.5665218566765553 1105 3 0.2995743467884786 1105 4 0.1444561240200528 1105 5 0.428861302331093 1105 6 0.29513411472982654 1105 7 0.4058118304036782 1105 8 0.33540222694115585 1105 9 0.26821053334400347 1105 10-11 0.24520869627602337 1105 12-13 0.30034941240531765 1105 14-15 0.26549432910974247 1105 16-17 0.4665220202563134 1105 18-19 0.23719826778609132 1105 20-21 0.24941261941658155 1105 22-23 0.32976575091275606 1105 24-25 0.21066965749818678 1105 26-27 0.25959294780198405 1105 28-29 0.23631547440523093 1105 30-31 0.19941061703362806 1105 32-33 0.14705495765585397 1105 34-35 0.09755281874139143 1105 36-37 0.2253094404561864 1105 38-39 0.12447067278173307 1105 40-41 0.1770368521363821 1105 42-43 0.23691397403515424 1105 44-45 0.2181507332575805 1105 46-47 0.24051469857826646 1105 48-49 0.15253473699005582 1105 50-51 0.24856333916421391 1105 52-53 0.22420760719585076 1105 54-55 0.2972991929803399 1105 56-57 0.2784920830502742 1105 58-59 0.32259876079523053 1105 60-61 0.1647338082729135 1105 62-63 0.2527565592614138 1105 64-65 0.20604837570060397 1105 66-67 0.1486616295658152 1105 68-69 0.1806414326691268 1105 70-71 0.2182409657611899 1105 72-73 0.256611275459953 1105 74-75 0.3324112838241291 1105 76-77 0.26316470350560905 1105 78-79 0.288733005088627 1105 80-81 0.2887325985394895 1105 82-83 0.25815040148238566 1105 84-85 0.24200647383853635 1105 86-87 0.27138918220514086 1105 88-89 0.23935402721959775 1105 90-91 0.22508180686922685 1105 92-93 0.24541268770609648 1105 94-95 0.3645973999709362 1105 96-97 0.2992718743754068 1105 98-99 0.2379892904110612 1105 100-101 0.2340902798102995 1105 102-103 0.2424808952449098 1105 104-105 0.27521424907821057 1105 106-107 0.2504271474634834 1105 108-109 0.2993806417817382 1105 110-111 0.2883412925035209 1105 112-113 0.19076745256317906 1105 114-115 0.3273353487567121 1105 116-117 0.3498126435830571 1105 118-119 0.41610626213705615 1105 120-121 0.3632526393258715 1105 122-123 0.3761463055604537 1105 124-125 0.36852453312451416 1106 1 0.1629595904907184 1106 2 0.5126983750455096 1106 3 0.29724196497409494 1106 4 0.12492097583838557 1106 5 0.3121609843845192 1106 6 0.21752033859205966 1106 7 0.2587255670882129 1106 8 0.2422148705392857 1106 9 0.15928250066745164 1106 10-11 0.12896963294199537 1106 12-13 0.174692052058127 1106 14-15 0.2045460592496937 1106 16-17 0.23390174594035784 1106 18-19 0.10229666877010146 1106 20-21 0.15110562691269536 1106 22-23 0.06310952774676082 1106 24-25 0.11712606168487127 1106 26-27 0.20246873976744695 1106 28-29 0.2066489701930152 1106 30-31 0.18311237386250667 1106 32-33 0.10187289262765375 1106 34-35 0.13163750392786255 1106 36-37 0.09158911740389897 1106 38-39 -0.06365912832760046 1106 40-41 -0.04371206289994234 1106 42-43 0.019010332734588076 1106 44-45 -0.05235682087456439 1106 46-47 0.05893413277260606 1106 48-49 0.03263232570179042 1106 50-51 0.062360944121401474 1106 52-53 0.0547264171009374 1106 54-55 0.07969203105967182 1106 56-57 0.07566827651032781 1106 58-59 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-0.9720069058777128 2216 76-77 -0.8112151418297984 2216 78-79 -0.7030031263014607 2216 80-81 -0.6276808657913548 2216 82-83 -0.7376998153585639 2216 84-85 -0.7281566777602784 2216 86-87 -0.6028351216794938 2216 88-89 -0.7077787730815892 2216 90-91 -0.7984921551620872 2216 92-93 -0.7066193465122197 2216 94-95 -0.7268519832933329 2216 96-97 -0.7775144992764247 2216 98-99 -0.8789947137978515 2216 100-101 -1.1195138211424194 2216 102-103 -0.9807600021411744 2216 104-105 -0.7909003198503441 2216 106-107 -0.6567224096435993 2216 108-109 -0.715666394725254 2216 110-111 -0.7893946401860887 2216 112-113 -0.6346392800849188 2216 114-115 -0.745268057950998 2216 116-117 -0.7271865085209583 2216 118-119 -0.7654474549697703 2216 120-121 -0.800645292982427 2216 122-123 -0.8345003398203232 2216 124-125 -1.0341588887391318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87.0 3 66.0 4 182.0 5 456.0 6 1118.0 7 2328.0 8 3530.0 9 5938.0 10 9144.0 11 11604.0 12 12515.0 13 15501.0 14 20890.0 15 15889.0 16 8281.0 17 7239.0 18 7128.0 19 6744.0 20 6906.0 21 7181.0 22 7990.0 23 9375.0 24 12077.0 25 15867.0 26 20613.0 27 26499.0 28 33345.0 29 42104.0 30 53204.0 31 66882.0 32 83553.0 33 106684.0 34 143706.0 35 205531.0 36 347109.0 37 497351.0 38 172252.0 39 580.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.89929214135422 17.56545766618617 10.336176213955675 23.199073978503936 2 19.653554669771495 20.661282190876516 35.447374061733626 24.237789077618366 3 18.421019529074233 26.079085379383706 31.233386349422148 24.266508742119917 4 12.174802976076366 15.751850739314568 35.491979919987884 36.58136636462118 5 11.463265310232465 41.015049934850076 34.46274974977192 13.058935005145536 6 32.58622745663155 36.16433545146109 17.117286649656567 14.132150442250788 7 27.89430068394208 33.617214831676186 21.483922354737153 17.004562129644583 8 28.511675016566464 32.097125511145194 19.453027473912538 19.938171998375807 9 27.154004958114648 11.216187182664813 18.942775386940745 42.68703247227979 10-11 25.24185526270108 24.806045337515577 28.915861488772794 21.036237911010545 12-13 26.28661163129217 23.89087719986777 28.800739037831914 21.02177213100814 14-15 23.21277174911155 25.27923986980295 26.39526850751894 25.11271987356657 16-17 22.81783543967828 27.828181624923428 26.71207667120767 22.641906264190624 18-19 21.919128490844294 25.430463876054176 29.90811336542472 22.742294267676805 20-21 22.808358856000833 25.01895646127272 30.032644862836733 22.14003981988972 22-23 22.66687094863818 25.235666424647878 29.899861581353786 22.19760104536016 24-25 22.36246624755308 25.51550392374947 29.884472478154777 22.23755735054267 26-27 22.354435258464495 25.443042815186708 29.72992011367336 22.472601812675443 28-29 22.16829211718137 25.478087739609922 29.97372511193998 22.379895031268727 30-31 22.12217772632153 25.038856342980374 30.16414509252816 22.674820838169936 32-33 22.02456515865313 25.476125425105252 29.799657752224086 22.69965166401754 34-35 22.5767813893671 25.510297268678446 29.570762856522535 22.34215848543192 36-37 22.766194252028605 25.26351619588729 29.040644564967454 22.929644987116653 38-39 22.542113030321783 25.329228573915607 29.18303815594765 22.94562023981496 40-41 22.690228876428463 25.457647193788212 29.22602251731869 22.626101412464628 42-43 22.910882291080682 25.41227356025026 28.866923217036984 22.809920931632078 44-45 22.565416466849065 25.712361282972417 28.801134645277422 22.921087604901096 46-47 23.02641709583041 25.99169785798899 27.968350410980687 23.013534635199914 48-49 23.168458976153115 26.106704246416072 27.404106347470275 23.32073042996054 50-51 23.005686948851174 26.125344348111817 27.594068142242406 23.2749005607946 52-53 23.289508245886115 25.88663277803888 27.59667165502437 23.227187321050636 54-55 22.942037981145543 26.230107501451105 27.472260388657737 23.35559412874561 56-57 23.12777995965937 26.29723902941968 27.4412634260148 23.133717584906154 58-59 23.338930362027288 25.78234284207624 27.45048214619969 23.428244649696783 60-61 22.99651830925535 25.97601938012873 27.850481773516258 23.176980537099666 62-63 22.84139661927425 26.014993860110135 27.876735264201436 23.266874256414177 64-65 22.714433897730277 26.09427435774804 27.841631396574872 23.349660347946806 66-67 22.74670207664637 26.540150458868855 27.593015351313056 23.120132113171717 68-69 23.072266862766952 25.900307358515583 27.804662178600026 23.22276360011744 70-71 22.75802468825201 25.999524500190578 27.746344752457063 23.49610605910035 72-73 22.869625160701123 26.311585324433867 27.406390810034292 23.41239870483072 74-75 22.863788925024398 26.522339316699263 27.367829703387773 23.246042054888573 76-77 22.73819021001751 26.40294913605741 27.448847871081718 23.410012782843367 78-79 22.997963969326456 26.36057189995902 27.324195808136626 23.317268322577902 80-81 22.88876857790064 26.577052311463678 27.082752603597992 23.45142650703769 82-83 22.9073739473283 26.46093498027365 27.20779916707045 23.423891905327597 84-85 22.758577313752003 26.125802698198058 27.29524827824774 23.820371709802195 86-87 22.76294335648625 26.19532629563129 27.17910516476359 23.862625183118872 88-89 22.802722532899537 26.311151073706835 26.998476412061102 23.887649981332526 90-91 22.725230324895215 26.420139779914564 27.075112835298203 23.77951705989202 92-93 22.793075991260082 26.08596769953193 27.17148884301731 23.949467466190676 94-95 22.5718251385532 26.061788349530683 27.27112400112005 24.09526251079607 96-97 22.974032688396033 26.04329017398338 27.047918073968408 23.934759063652177 98-99 22.784422241907247 26.158964139517227 26.72374428936548 24.332869329210045 100-101 22.600196334650448 25.936124317772315 26.996517600933473 24.46716174664377 102-103 22.661999643255992 25.698676097321176 27.06585096905812 24.573473290364717 104-105 22.49927481667894 25.67395506303749 27.139341113403574 24.687429006879995 106-107 22.525519734632496 25.80276311270949 27.23310099839869 24.438616154259325 108-109 22.718070548088747 25.89227917840401 26.59693487727458 24.792715396232662 110-111 22.522149909166313 25.945526959905628 26.836831706981425 24.695491423946635 112-113 22.515713649141976 25.92452063966208 26.86581735004256 24.69394836115339 114-115 22.19202625036487 26.18818632927701 26.699227989652847 24.92055943070528 116-117 22.104073878362396 26.049092876374523 27.007775345361214 24.839057899901864 118-119 22.13747660640231 26.296542503002986 26.524477540888157 25.041503349706545 120-121 22.20762674124703 26.210123600045694 26.655275985421405 24.926973673285875 122-123 22.171575246737323 26.16928971705374 26.43200989490438 25.22712514130456 124-125 22.2155800711314 26.14431180269993 26.14275190358012 25.497356222588547 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 42.0 1 40.0 2 156.5 3 321.0 4 305.5 5 288.0 6 457.0 7 980.0 8 1598.5 9 2089.5 10 2109.0 11 1824.5 12 1903.5 13 2125.0 14 2326.0 15 2590.5 16 2851.0 17 3083.5 18 3374.0 19 3611.0 20 3999.0 21 4511.5 22 5136.0 23 6214.0 24 7542.0 25 9129.5 26 11027.5 27 12691.0 28 14330.5 29 16804.0 30 20528.0 31 24501.0 32 29140.0 33 31496.0 34 33431.0 35 35807.5 36 38096.5 37 42836.5 38 46207.5 39 47918.5 40 49228.5 41 52977.5 42 57307.0 43 61594.5 44 67562.5 45 71874.5 46 73640.0 47 74231.0 48 74619.0 49 73560.0 50 73185.5 51 71987.5 52 69984.0 53 69917.5 54 69479.5 55 67006.5 56 61569.0 57 56353.5 58 53157.0 59 49178.0 60 42637.5 61 35611.0 62 30183.5 63 24170.0 64 19015.0 65 15968.0 66 12290.5 67 9585.0 68 7314.5 69 4535.5 70 3159.0 71 2549.0 72 2199.5 73 1929.0 74 1471.5 75 1126.5 76 767.0 77 486.0 78 295.0 79 158.5 80 93.0 81 47.5 82 16.5 83 9.5 84 8.0 85 5.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06631616710667797 2 3.522102957107327E-4 3 1.0063151306020935E-4 4 0.0 5 0.06344816898446198 6 1.0063151306020935E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.5473634795157E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.515787826505234E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.2642090438547102E-4 36-37 0.0 38-39 0.0 40-41 5.031575653010468E-5 42-43 0.002767366609155757 44-45 0.011069466436623028 46-47 0.012629254889056274 48-49 0.010490835236526823 50-51 0.009031678297153789 52-53 0.007798942262166223 54-55 0.008729783757973161 56-57 0.006163680174937822 58-59 0.004276839305058897 60-61 0.0026415772178304954 62-63 0.0017862093568187156 64-65 0.002389998435179972 66-67 0.0031195769048664897 68-69 0.0015346305741681925 70-71 0.003345997809251961 72-73 0.0032202084179266993 74-75 0.001132104521927355 76-77 9.81157252337041E-4 78-79 1.7610514785536635E-4 80-81 3.522102957107327E-4 82-83 3.01894539180628E-4 84-85 5.786312000962037E-4 86-87 5.28315443566099E-4 88-89 0.0017610514785536635 90-91 0.001962314504674082 92-93 8.30209982746727E-4 94-95 0.0017862093568187156 96-97 0.0023396826786498673 98-99 0.00193715662640903 100-101 0.0013082096697827215 102-103 0.001484314817638088 104-105 0.0010314730088671457 106-107 8.30209982746727E-4 108-109 0.0012327360349875644 110-111 0.0012830517915176692 112-113 0.012251886715080486 114-115 0.021585459551414905 116-117 0.020075986855511766 118-119 0.013082096697827214 120-121 0.00764799499257591 122-123 0.008226626192672115 124-125 0.0070945216707447595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1987449.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.453047273529453 #Duplication Level Percentage of deduplicated Percentage of total 1 41.07718550574799 10.044623590355945 2 16.64705400025327 8.141423968663815 3 12.174060538076413 8.930786335451756 4 8.496071892596492 8.310193905158675 5 5.744960091110081 7.024089034622744 6 3.9572467591075995 5.806004524408761 7 2.6216359919593035 4.487489219175698 8 1.848976604643469 3.6170489856797383 9 1.2747239898125169 2.8053797385228796 >10 5.622899582075611 23.667381481668784 >50 0.3379865013231216 5.714794980756565 >100 0.18467864272282256 8.2429474638307 >500 0.009389925428451022 1.5990710152769396 >1k 0.0029213101332958733 1.2728174630997562 >5k 2.0866500952113382E-4 0.3359482933272718 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6584 0.3312789409942092 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4971 0.2501196257111503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4948 0.24896236331095795 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2142 0.1077763504874842 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.031575653010467E-5 7 0.0 0.0 0.0 0.0 5.031575653010467E-5 8 0.0 0.0 0.0 0.0 5.031575653010467E-5 9 0.0 0.0 0.0 0.0 5.031575653010467E-5 10-11 0.0 0.0 0.0 0.0 5.031575653010467E-5 12-13 0.0 0.0 0.0 0.0 5.031575653010467E-5 14-15 0.0 0.0 0.0 0.0 5.031575653010467E-5 16-17 0.0 0.0 0.0 0.0 5.031575653010467E-5 18-19 1.50947269590314E-4 0.0 0.0 5.031575653010467E-5 5.031575653010467E-5 20-21 3.01894539180628E-4 0.0 0.0 7.5473634795157E-5 5.031575653010467E-5 22-23 3.522102957107327E-4 0.0 0.0 1.0063151306020934E-4 5.031575653010467E-5 24-25 3.522102957107327E-4 0.0 0.0 1.50947269590314E-4 5.031575653010467E-5 26-27 3.522102957107327E-4 0.0 0.0 2.2642090438547102E-4 5.031575653010467E-5 28-29 3.522102957107327E-4 0.0 0.0 4.276839305058897E-4 5.031575653010467E-5 30-31 3.522102957107327E-4 0.0 0.0 0.0018365251133488207 5.031575653010467E-5 32-33 3.522102957107327E-4 0.0 0.0 0.006390101079323293 5.031575653010467E-5 34-35 3.7736817397578505E-4 0.0 0.0 0.012503465497731011 5.031575653010467E-5 36-37 4.0252605224083736E-4 0.0 0.0 0.02412640525618519 5.031575653010467E-5 38-39 5.031575653010467E-4 0.0 0.0 0.04221491972875782 5.031575653010467E-5 40-41 5.031575653010467E-4 0.0 0.0 0.062039327801619054 5.031575653010467E-5 42-43 5.031575653010467E-4 0.0 0.0 0.08732299545799665 5.031575653010467E-5 44-45 5.031575653010467E-4 0.0 0.0 0.12100939445490172 5.031575653010467E-5 46-47 6.03789078361256E-4 0.0 0.0 0.1650356814187433 5.031575653010467E-5 48-49 6.03789078361256E-4 0.0 0.0 0.20888586323472955 5.031575653010467E-5 50-51 6.03789078361256E-4 0.0 0.0 0.25344046564213724 5.031575653010467E-5 52-53 6.03789078361256E-4 0.0 0.0 0.3040581167114225 5.031575653010467E-5 54-55 6.03789078361256E-4 0.0 0.0 0.3656446027042707 5.031575653010467E-5 56-57 6.03789078361256E-4 2.5157878265052335E-5 0.0 0.4467787601090645 5.031575653010467E-5 58-59 6.03789078361256E-4 5.031575653010467E-5 0.0 0.5412969087508661 5.031575653010467E-5 60-61 6.03789078361256E-4 5.031575653010467E-5 0.0 0.6302803241743562 5.031575653010467E-5 62-63 6.03789078361256E-4 5.031575653010467E-5 0.0 0.719163108084786 5.031575653010467E-5 64-65 6.03789078361256E-4 5.031575653010467E-5 0.0 0.8245243022588253 5.031575653010467E-5 66-67 0.0011572624001924075 5.031575653010467E-5 0.0 0.955647163776278 5.031575653010467E-5 68-69 0.0011572624001924075 5.031575653010467E-5 0.0 1.1257144208480319 5.031575653010467E-5 70-71 0.0011572624001924075 5.031575653010467E-5 0.0 1.287404104457523 5.031575653010467E-5 72-73 0.0011572624001924075 5.031575653010467E-5 0.0 1.4530687328328928 5.031575653010467E-5 74-75 0.0011572624001924075 5.031575653010467E-5 0.0 1.6281927234359221 5.031575653010467E-5 76-77 0.001283051791517669 5.031575653010467E-5 0.0 1.833858378252725 5.031575653010467E-5 78-79 0.0014088411828429308 5.031575653010467E-5 0.0 2.089714000208307 5.031575653010467E-5 80-81 0.0015094726959031402 5.031575653010467E-5 0.0 2.3574692985832595 5.031575653010467E-5 82-83 0.0015094726959031402 5.031575653010467E-5 0.0 2.6177023913569606 5.031575653010467E-5 84-85 0.0016352620872284018 5.031575653010467E-5 0.0 2.89869073369933 5.031575653010467E-5 86-87 0.0017610514785536635 5.031575653010467E-5 0.0 3.2084093730203893 5.031575653010467E-5 88-89 0.0017610514785536635 5.031575653010467E-5 0.0 3.5690727158281796 5.031575653010467E-5 90-91 0.0017610514785536635 5.031575653010467E-5 0.0 3.961912984936972 5.031575653010467E-5 92-93 0.0017610514785536635 5.031575653010467E-5 0.0 4.34785999540114 5.031575653010467E-5 94-95 0.0017610514785536635 5.031575653010467E-5 0.0 4.7571535168952765 5.031575653010467E-5 96-97 0.0017610514785536635 5.031575653010467E-5 0.0 5.197517018046753 5.031575653010467E-5 98-99 0.0018113672350837681 5.031575653010467E-5 0.0 5.695265639520813 5.031575653010467E-5 100-101 0.0018113672350837681 5.031575653010467E-5 0.0 6.255430956970468 5.031575653010467E-5 102-103 0.0018113672350837681 5.031575653010467E-5 0.0 6.784652084154109 5.031575653010467E-5 104-105 0.0018113672350837681 5.031575653010467E-5 0.0 7.340590878055236 5.031575653010467E-5 106-107 0.002012630261204187 5.031575653010467E-5 0.0 7.919926498742861 5.031575653010467E-5 108-109 0.0021132617742643965 5.031575653010467E-5 0.0 8.539137356480595 5.031575653010467E-5 110-111 0.0022138932873246057 5.031575653010467E-5 0.0 9.216337123619272 5.031575653010467E-5 112-113 0.0022138932873246057 5.031575653010467E-5 0.0 9.875272271137524 5.031575653010467E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4600 0.0 83.800896 1 GTATCAA 7300 0.0 52.68195 2 GTACATG 13035 0.0 49.410233 1 TACATGG 13565 0.0 47.178146 2 ACATGGG 13495 0.0 46.275894 3 CATGGGA 8690 0.0 42.74812 4 ATCAACG 10675 0.0 37.81064 4 TCAACGC 10695 0.0 37.628605 5 ATGGGAT 3355 0.0 37.617985 5 CAACGCA 10845 0.0 37.302376 6 AACGCAG 11080 0.0 36.67229 7 TATCAAC 11395 0.0 35.369305 3 ATGGGAG 3535 0.0 35.365685 5 CATGGGG 5315 0.0 34.834476 4 GTACCGT 120 9.303003E-7 34.70355 6 GAGTACA 8715 0.0 31.721434 1 ACGCAGA 13015 0.0 30.90008 8 AGTACAT 8535 0.0 30.829815 2 CGCAGAG 13300 0.0 30.193205 9 TATAACG 165 3.393743E-7 28.864115 2 >>END_MODULE