FastQCFastQC Report
Thu 26 May 2016
SRR936202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080188
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.056.58241
GTATCAA29200.048.08342
GTACATG92500.047.7873651
TACATGG93300.046.166072
ACATGGG92300.045.11933
ATCAACG33750.042.4824184
TCAACGC33750.042.3061455
CATGGGG56400.040.4004024
CAACGCA35200.040.3944056
AACGCAG36850.038.424257
TATCAAC40850.034.5161443
CTAACGT700.002791108333.996014
ACGCAGA43900.031.711548
CGCAGAG44150.031.5319759
ATGGGGA36050.030.6954275
CATGGGA40700.029.965774
ATGGGGG30900.028.8801045
GAGTACA61700.026.1306441
AGTACAT62650.024.8797632
CATGGGC22500.022.2107264
Produced by FastQC (version 0.11.5)