##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936202_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2080188 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.12258843912185 31.0 30.0 34.0 26.0 34.0 2 30.09795028141687 31.0 30.0 34.0 26.0 34.0 3 30.104818891369433 31.0 30.0 34.0 26.0 34.0 4 33.5837717552452 35.0 35.0 37.0 28.0 37.0 5 33.705255486523335 37.0 35.0 37.0 28.0 37.0 6 33.77016692722004 37.0 35.0 37.0 29.0 37.0 7 33.59522600841847 37.0 35.0 37.0 28.0 37.0 8 33.58520912532906 37.0 35.0 37.0 28.0 37.0 9 34.98109690085704 39.0 35.0 39.0 28.0 39.0 10-11 34.82366665897506 38.5 35.0 39.0 27.0 39.0 12-13 34.85413049205168 39.0 35.0 39.0 27.0 39.0 14-15 36.021699721371334 40.0 36.0 41.0 27.0 41.0 16-17 36.0572948214296 40.0 36.0 41.0 27.0 41.0 18-19 36.07628084576971 40.0 36.0 41.0 27.0 41.0 20-21 36.13978351956651 40.0 36.0 41.0 27.0 41.0 22-23 36.0318043369157 39.5 36.0 41.0 27.0 41.0 24-25 35.99224397025654 39.0 36.0 41.0 27.0 41.0 26-27 36.0684541493365 39.0 36.0 41.0 28.0 41.0 28-29 35.94653872630743 39.0 35.0 41.0 27.5 41.0 30-31 35.86930219768598 39.0 35.0 41.0 27.0 41.0 32-33 35.68399034125762 39.0 35.0 41.0 27.0 41.0 34-35 35.64081179201111 39.0 35.0 41.0 27.0 41.0 36-37 35.54424047249576 39.0 35.0 41.0 27.0 41.0 38-39 35.30794115724156 39.0 34.0 41.0 26.0 41.0 40-41 35.401831709441645 39.0 34.5 40.5 26.5 41.0 42-43 35.33194595873066 39.0 35.0 41.0 26.0 41.0 44-45 35.239124059940735 39.0 34.0 41.0 26.0 41.0 46-47 35.214376056394904 39.0 34.0 41.0 26.0 41.0 48-49 35.118311902578036 39.0 34.0 41.0 26.0 41.0 50-51 34.981239676413864 39.0 34.0 40.0 26.0 41.0 52-53 34.82426732583786 38.5 34.0 40.0 25.5 41.0 54-55 34.64610674612102 38.0 33.5 40.0 25.0 41.0 56-57 34.44214657521339 38.0 33.0 40.0 24.5 41.0 58-59 34.22265511578761 38.0 33.0 40.0 24.0 41.0 60-61 33.98356759100619 38.0 33.0 40.0 23.0 41.0 62-63 33.73898777418195 37.0 33.0 40.0 23.0 41.0 64-65 33.45898639930621 37.0 32.0 40.0 22.0 41.0 66-67 33.16431086997906 36.0 32.0 39.5 22.0 41.0 68-69 32.87780960182445 36.0 32.0 39.0 21.5 41.0 70-71 32.531128917194025 35.5 31.5 39.0 20.0 40.5 72-73 32.19543209556059 35.0 31.0 38.5 19.5 40.0 74-75 31.85881252079139 35.0 31.0 37.5 19.0 40.0 76-77 31.524340348083925 35.0 31.0 37.0 18.0 39.0 78-79 31.178648035658316 35.0 31.0 36.5 17.5 39.0 80-81 30.855791399623495 35.0 31.0 36.0 15.5 38.5 82-83 30.545157697285052 34.0 30.0 36.0 12.5 37.0 84-85 30.564046134291708 35.0 31.0 35.0 13.5 37.0 86-87 30.43369517562836 35.0 31.0 35.0 11.5 37.0 88-89 30.25171883502837 34.5 31.0 35.0 9.0 36.0 90-91 30.080792937945994 34.0 31.0 35.0 7.0 36.0 92-93 29.95637221251156 34.0 31.0 35.0 7.0 36.0 94-95 29.819559578268887 34.0 30.5 35.0 4.5 35.5 96-97 29.706902693410402 34.0 30.0 35.0 2.0 35.0 98-99 29.591891934767435 34.0 30.0 35.0 2.0 35.0 100-101 29.483990148967305 34.0 30.0 35.0 2.0 35.0 102-103 28.536341186469684 33.5 28.5 34.5 2.0 35.0 104-105 29.182643347620505 34.0 29.5 35.0 2.0 35.0 106-107 29.300094751051347 34.0 30.0 35.0 2.0 35.0 108-109 29.24063594252058 34.0 30.0 35.0 2.0 35.0 110-111 29.118198932019606 34.0 30.0 35.0 2.0 35.0 112-113 29.071475270504397 34.0 30.0 35.0 2.0 35.0 114-115 28.953018909829304 34.0 30.0 35.0 2.0 35.0 116-117 28.817954194524724 34.0 30.0 35.0 2.0 35.0 118-119 28.602404926862377 34.0 29.0 35.0 2.0 35.0 120-121 28.395409693739218 34.0 29.0 35.0 2.0 35.0 122-123 28.09464553203845 34.0 29.0 35.0 2.0 35.0 124-125 26.119824265883658 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5694945187046514 1101 2 0.7796441051036425 1101 3 0.736517238693132 1101 4 0.7332617803026267 1101 5 0.6235792458282461 1101 6 0.6914536469988946 1101 7 0.6453332591217631 1101 8 0.6339845984949761 1101 9 0.7660547012084606 1101 10-11 0.9740889390476397 1101 12-13 0.8741505277030157 1101 14-15 1.0015726264437816 1101 16-17 0.9781435578131479 1101 18-19 0.9140984883034307 1101 20-21 0.7922118960050355 1101 22-23 0.9056535569789048 1101 24-25 1.093352004169553 1101 26-27 0.9346029491842032 1101 28-29 0.9928249191169556 1101 30-31 0.8774544305474095 1101 32-33 0.9135494825041093 1101 34-35 0.8978870167950959 1101 36-37 1.0346306075662142 1101 38-39 1.1018948434298679 1101 40-41 1.0460711863413792 1101 42-43 1.1611235415461323 1101 44-45 1.0413655068695675 1101 46-47 0.9919956093910685 1101 48-49 0.9766215514268524 1101 50-51 0.9812763769807162 1101 52-53 1.0132789888623677 1101 54-55 0.9598161135621339 1101 56-57 0.9437151668532877 1101 58-59 0.9534500422411867 1101 60-61 1.0376214218495292 1101 62-63 1.0331490065721027 1101 64-65 1.0486172234734568 1101 66-67 1.0674695903088534 1101 68-69 1.1051520192184654 1101 70-71 1.0459216845506845 1101 72-73 1.1148194272178458 1101 74-75 1.1286333656743324 1101 76-77 1.0659735669367834 1101 78-79 1.070329731624014 1101 80-81 1.1064968025436421 1101 82-83 1.030583365057268 1101 84-85 0.9443112542868235 1101 86-87 1.0126799827482316 1101 88-89 0.9451060176693531 1101 90-91 0.8610087345250044 1101 92-93 0.9264359189767859 1101 94-95 0.9663734603614529 1101 96-97 0.9929224664020566 1101 98-99 1.0039981883682145 1101 100-101 1.031927403062781 1101 102-103 1.0514375749690466 1101 104-105 1.0095702556814352 1101 106-107 0.9637152245214473 1101 108-109 0.9204882163397485 1101 110-111 1.0192527631363077 1101 112-113 1.083408595009029 1101 114-115 1.0739361794930886 1101 116-117 1.15438560330427 1101 118-119 1.1537999882183954 1101 120-121 1.292790714558194 1101 122-123 1.4114519453514554 1101 124-125 1.0305407533494133 1102 1 0.35224512820855125 1102 2 0.303172913411327 1102 3 0.43273275729996286 1102 4 0.5089769946341747 1102 5 0.3441784546846023 1102 6 0.28851197512456395 1102 7 0.3292109102300387 1102 8 0.36424193177114006 1102 9 0.5229509798422214 1102 10-11 0.5453159557538143 1102 12-13 0.49173755541016106 1102 14-15 0.6610628204833375 1102 16-17 0.5876881475121323 1102 18-19 0.575064531404955 1102 20-21 0.5347358992465416 1102 22-23 0.5783715179262003 1102 24-25 0.7091157518737745 1102 26-27 0.6251516835934083 1102 28-29 0.5547043182153573 1102 30-31 0.6395828675490378 1102 32-33 0.7325306146462722 1102 34-35 0.6261671420246344 1102 36-37 0.565761136036059 1102 38-39 0.6644240420916319 1102 40-41 0.6873944764356565 1102 42-43 0.8228680141109947 1102 44-45 0.8011589810087685 1102 46-47 0.6506628696960419 1102 48-49 0.6540871926878324 1102 50-51 0.5494084169216507 1102 52-53 0.6713457902850593 1102 54-55 0.5874354363959284 1102 56-57 0.6437669350400697 1102 58-59 0.7430673771245253 1102 60-61 0.74631152954057 1102 62-63 0.7417029972287139 1102 64-65 0.6640274017165524 1102 66-67 0.6290368549572918 1102 68-69 0.6953156730963315 1102 70-71 0.6046157497270812 1102 72-73 0.5380565814110554 1102 74-75 0.4316028190028405 1102 76-77 0.45665596297234146 1102 78-79 0.45869186004241413 1102 80-81 0.44398324780752674 1102 82-83 0.3185048872391363 1102 84-85 0.3355282131206998 1102 86-87 0.27190341158236464 1102 88-89 0.4417655000972367 1102 90-91 0.44352583844187166 1102 92-93 0.4859845870713748 1102 94-95 0.48776945843693653 1102 96-97 0.5372681592117203 1102 98-99 0.44705257835416745 1102 100-101 0.5206958178094787 1102 102-103 0.5500643136002523 1102 104-105 0.5160394653757976 1102 106-107 0.5060937541356694 1102 108-109 0.5552103650599101 1102 110-111 0.49409640991208903 1102 112-113 0.4277711594483762 1102 114-115 0.5146081725971108 1102 116-117 0.5302539830148945 1102 118-119 0.569755090337793 1102 120-121 0.5358617901580658 1102 122-123 0.48003842180088085 1102 124-125 0.6203464198917956 1103 1 0.2685366592175775 1103 2 0.23914444909415167 1103 3 0.24997175572885055 1103 4 0.31814852960314965 1103 5 0.24283852368508008 1103 6 0.20702590723411163 1103 7 0.1319613300122171 1103 8 0.1626318016572199 1103 9 0.291576116540881 1103 10-11 0.4774089736798288 1103 12-13 0.2756580546722276 1103 14-15 0.3807055268401953 1103 16-17 0.27695611332993053 1103 18-19 0.3635610485536773 1103 20-21 0.24131569430398514 1103 22-23 0.40072985359282143 1103 24-25 0.49639897008263034 1103 26-27 0.2718467343011781 1103 28-29 0.32215645055477893 1103 30-31 0.3523749786027821 1103 32-33 0.43146181263608696 1103 34-35 0.3703434714851497 1103 36-37 0.24992789248105396 1103 38-39 0.3669251145004324 1103 40-41 0.33441348054335407 1103 42-43 0.43657640889982474 1103 44-45 0.3113925834391438 1103 46-47 0.38730941944204034 1103 48-49 0.25397285564122996 1103 50-51 0.2767688034791149 1103 52-53 0.16791586674050052 1103 54-55 0.22595707425697498 1103 56-57 0.2811216737813709 1103 58-59 0.2611239046916083 1103 60-61 0.18568293220430832 1103 62-63 0.2210051552054466 1103 64-65 0.1675716810930652 1103 66-67 0.31716295313724174 1103 68-69 0.28692544910636286 1103 70-71 0.3182727853628009 1103 72-73 0.2834328722113142 1103 74-75 0.2644094736763094 1103 76-77 0.2833721246589924 1103 78-79 0.21386396063834923 1103 80-81 0.21065367467554452 1103 82-83 0.14997327341676225 1103 84-85 0.10566960952798254 1103 86-87 0.23060686723244928 1103 88-89 0.26494564796237796 1103 90-91 0.25079958319759044 1103 92-93 0.29569404060265825 1103 94-95 0.3321785571974516 1103 96-97 0.41588369377756607 1103 98-99 0.3221088522250639 1103 100-101 0.3745752701375622 1103 102-103 0.3005971366357514 1103 104-105 0.340990884106656 1103 106-107 0.21993737528282864 1103 108-109 0.18332754771167714 1103 110-111 0.18871181410759164 1103 112-113 0.3381663910496968 1103 114-115 0.311207657521539 1103 116-117 0.28144372981934396 1103 118-119 0.18560337156728934 1103 120-121 0.17178137494918388 1103 122-123 0.20123660228923157 1103 124-125 0.08522354067588012 1104 1 0.26795561834224557 1104 2 0.33620398756704617 1104 3 0.3472688719861736 1104 4 0.439946044638198 1104 5 0.3315470702043086 1104 6 0.27563806776306876 1104 7 0.20229393701283271 1104 8 0.2507245814747918 1104 9 0.41751046423520677 1104 10-11 0.366738476798794 1104 12-13 0.3651587959642484 1104 14-15 0.42391527102091686 1104 16-17 0.2909846355676464 1104 18-19 0.25042259858173566 1104 20-21 0.24019901230166596 1104 22-23 0.3613176062303296 1104 24-25 0.33461732266287214 1104 26-27 0.22675928119765132 1104 28-29 0.3590038730540357 1104 30-31 0.292367327535473 1104 32-33 0.43017619802786555 1104 34-35 0.37689237332357806 1104 36-37 0.3404841186224701 1104 38-39 0.5801894598246733 1104 40-41 0.44524327978064093 1104 42-43 0.522013611797135 1104 44-45 0.4863082784310109 1104 46-47 0.4215214268737526 1104 48-49 0.43291375778783703 1104 50-51 0.47160270112294 1104 52-53 0.6037135540763714 1104 54-55 0.6066019897792927 1104 56-57 0.4948312402531485 1104 58-59 0.5166697899373602 1104 60-61 0.5536229866991889 1104 62-63 0.6043731068352187 1104 64-65 0.6294887402596032 1104 66-67 0.5499177851980264 1104 68-69 0.5806100167783654 1104 70-71 0.6069760471046308 1104 72-73 0.601036987544326 1104 74-75 0.5229628431915501 1104 76-77 0.48267336702760844 1104 78-79 0.5200015322176696 1104 80-81 0.46871628097594353 1104 82-83 0.570442639450313 1104 84-85 0.6459417006090789 1104 86-87 0.46701222806509435 1104 88-89 0.5287562324321939 1104 90-91 0.5162700811412719 1104 92-93 0.4510686781874256 1104 94-95 0.4894107418523177 1104 96-97 0.36736559469586894 1104 98-99 0.4572204483170239 1104 100-101 0.47935959713871057 1104 102-103 0.4353062072682725 1104 104-105 0.44901031083449894 1104 106-107 0.5051916793402675 1104 108-109 0.520929232236842 1104 110-111 0.6129300038749363 1104 112-113 0.5716512244976144 1104 114-115 0.5255474597892658 1104 116-117 0.4762942313418712 1104 118-119 0.5550051366639615 1104 120-121 0.5259543471364552 1104 122-123 0.5016669938010985 1104 124-125 0.6150377024750782 1105 1 0.1906154022577624 1105 2 0.2565900538766641 1105 3 0.2592044480161917 1105 4 0.26845185514719816 1105 5 0.16701595608638087 1105 6 0.15246518206727444 1105 7 0.04398819665201614 1105 8 0.1028580951187621 1105 9 0.19313478488260927 1105 10-11 0.3824581553589894 1105 12-13 0.2577282262154128 1105 14-15 0.2528961894594275 1105 16-17 0.2883701559928582 1105 18-19 0.31948871120837197 1105 20-21 0.25885166460876974 1105 22-23 0.19293213052551295 1105 24-25 0.3861652394504276 1105 26-27 0.3344611299976421 1105 28-29 0.3999462203422297 1105 30-31 0.30864785890238977 1105 32-33 0.5355617339758538 1105 34-35 0.5138788696141958 1105 36-37 0.4974882554432014 1105 38-39 0.5680016755868778 1105 40-41 0.4960229113807344 1105 42-43 0.5659731069520006 1105 44-45 0.5999430629101568 1105 46-47 0.3334278976922178 1105 48-49 0.29224328089478746 1105 50-51 0.3115670948235021 1105 52-53 0.3639462072778912 1105 54-55 0.4640471583326544 1105 56-57 0.4660836070150367 1105 58-59 0.36072360445715645 1105 60-61 0.3574226304883581 1105 62-63 0.37352470562606754 1105 64-65 0.41978245711893436 1105 66-67 0.4513567283776325 1105 68-69 0.3222434234260305 1105 70-71 0.30076057835919556 1105 72-73 0.401113566589828 1105 74-75 0.39006475134770113 1105 76-77 0.36399515757283396 1105 78-79 0.3095631837083559 1105 80-81 0.32134735514119583 1105 82-83 0.23187418713425956 1105 84-85 0.2828684157191361 1105 86-87 0.3361768179440219 1105 88-89 0.2887730450807453 1105 90-91 0.35199555487247736 1105 92-93 0.3225481025584145 1105 94-95 0.2488275251554235 1105 96-97 0.23747365541654375 1105 98-99 0.15184071493185058 1105 100-101 0.17866894432822278 1105 102-103 0.2519174107974145 1105 104-105 0.23006852827981206 1105 106-107 0.3409119788116435 1105 108-109 0.2752169380846041 1105 110-111 0.3032201541908819 1105 112-113 0.2306215800542688 1105 114-115 0.21366645372096826 1105 116-117 0.16811014152558812 1105 118-119 0.2334366226263782 1105 120-121 0.2502772675182712 1105 122-123 0.2760961528249517 1105 124-125 0.30761940311383995 1106 1 -0.21243919775307063 1106 2 -0.2418940542914214 1106 3 -0.07452365736432398 1106 4 -0.006650002242061248 1106 5 0.03281206330023423 1106 6 -0.0783787984387061 1106 7 -0.29663004427875705 1106 8 -0.2919849909718195 1106 9 -0.27508364871953717 1106 10-11 -0.057280425114043965 1106 12-13 -0.1219022748085834 1106 14-15 -0.25130212782130457 1106 16-17 -0.17487623812151298 1106 18-19 -0.16122604961849873 1106 20-21 -0.2569504795442157 1106 22-23 -0.3105501437627254 1106 24-25 -4.232978724403814 1106 26-27 -1.194535738636553 1106 28-29 -0.8643305023694836 1106 30-31 -0.7234892211997703 1106 32-33 -5.631173646189907 1106 34-35 -1.2791057785042952 1106 36-37 -0.45563559610208415 1106 38-39 -0.043391204946836126 1106 40-41 -0.6663421625735992 1106 42-43 -0.3008872615639291 1106 44-45 -0.22718412372482533 1106 46-47 -0.362455001008847 1106 48-49 -0.20315232191547494 1106 50-51 -0.13451638163635948 1106 52-53 -0.07508344321252025 1106 54-55 -0.28003066820875944 1106 56-57 -0.2152015716063218 1106 58-59 -0.09187000868010387 1106 60-61 -0.02330377561234087 1106 62-63 -0.145281362902125 1106 64-65 -0.08430314631326041 1106 66-67 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-1.7005225102204449 2216 80-81 -1.8343158722664192 2216 82-83 -1.7688186335899445 2216 84-85 -2.0713467820857794 2216 86-87 -1.9973250434138698 2216 88-89 -1.9614871913048475 2216 90-91 -1.8749464546432932 2216 92-93 -1.731541181982827 2216 94-95 -1.8315885859673173 2216 96-97 -1.9189431205542142 2216 98-99 -1.7927922795768367 2216 100-101 -1.8151897837691493 2216 102-103 -1.7955827678669571 2216 104-105 -1.77294359057856 2216 106-107 -1.8460144453960439 2216 108-109 -1.79865130434165 2216 110-111 -1.815934904104239 2216 112-113 -1.9267466288047395 2216 114-115 -1.8621228818569193 2216 116-117 -1.7554800702656834 2216 118-119 -1.8105530718683767 2216 120-121 -1.7489362483150046 2216 122-123 -1.6992066562120662 2216 124-125 -1.7576651893761905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49555.0 3 8537.0 4 5557.0 5 4864.0 6 5539.0 7 6212.0 8 6722.0 9 7192.0 10 8557.0 11 9720.0 12 10787.0 13 11334.0 14 11281.0 15 10615.0 16 9435.0 17 8829.0 18 7935.0 19 7699.0 20 7650.0 21 8041.0 22 9360.0 23 12568.0 24 17999.0 25 25131.0 26 29531.0 27 30410.0 28 35533.0 29 43706.0 30 51441.0 31 63781.0 32 81287.0 33 106513.0 34 145332.0 35 209003.0 36 340451.0 37 488705.0 38 182787.0 39 589.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.43225105519714 18.041082983203378 9.540424386351182 23.986241575248297 2 19.313762958583013 20.562065393222237 36.7650954121869 23.359076236007855 3 17.2813392648542 26.20150313481583 31.00006441731653 25.51709318301344 4 11.510505350984387 16.388261004587108 37.316470032852955 34.78476361157555 5 11.620862585834578 40.53300605144594 35.7623245090291 12.083806853690382 6 32.36982326461465 37.30576125435781 17.68517218768448 12.63924329334305 7 26.888103803104457 34.22669023526454 22.24184669610979 16.643359265521216 8 25.708023821290325 34.17878274514027 20.440341974351924 19.672851459217483 9 25.899109723811993 13.43841595353392 19.386426208607972 41.27604811404611 10-11 23.805840458237288 25.94451348343505 30.077110009158016 20.172536049169647 12-13 24.780555108202112 25.33672093397784 28.73404851127451 21.148675446545536 14-15 22.35839534717697 27.123495905391703 25.505363048699024 25.012745698732303 16-17 22.794140446881904 29.027349084153375 26.103894262098255 22.074616206866466 18-19 21.771950353007462 28.148751566010905 27.775721278422555 22.303576802559075 20-21 23.057887494065827 27.634082291222228 27.291745037828484 22.016285176883464 22-23 22.65837375414443 27.819927481925216 27.241096494847444 22.28060226908291 24-25 22.575557085906787 27.64028253007921 27.255258979896713 22.528901404117285 26-27 22.440420047438977 27.712213469631457 27.08535843157163 22.762008051357935 28-29 22.426307544869996 27.746720967277096 27.345283281458034 22.481688206394878 30-31 22.600187726169775 27.60202052008081 27.245244650945903 22.552547102803512 32-33 22.102916913444453 27.756588005445774 27.47843418532563 22.66206089578414 34-35 22.764698798626934 27.74373528209228 27.110779619755487 22.3807862995253 36-37 22.79849387490701 27.519977309574085 26.979012557852773 22.70251625766613 38-39 22.224174528840443 27.704184364619945 27.386901154982997 22.684739951556622 40-41 22.396931761203874 27.67299104728455 27.50040742234578 22.4296697691658 42-43 22.411912022711817 27.616944062460863 27.47769932992829 22.493444584899024 44-45 22.449393121271907 27.69559628334852 27.37886462143151 22.476145973948057 46-47 22.433439055257033 27.787543401473698 26.935197949178963 22.84381959409031 48-49 22.550877244428783 27.60027564942143 27.246916550671013 22.601930555478773 50-51 22.44426005612093 27.696186478770052 27.26097768863895 22.598575776470074 52-53 22.59964113208046 27.69491659866284 27.114406275501434 22.591035993755266 54-55 22.421556137778527 27.490253106747108 27.418263106026007 22.669927649448358 56-57 22.427468728885284 27.765269358598122 27.31869195292946 22.48856995958714 58-59 22.70439435238466 27.42179725695496 27.27055961772355 22.603248772936826 60-61 22.5240684437242 27.571200601491757 27.358693063014577 22.546037891769462 62-63 22.2459651395751 27.789981744126933 27.342009780532916 22.622043335765046 64-65 22.314306440703387 27.70525935252127 27.20779785795426 22.772636348821077 66-67 22.216952911996295 28.071063098160522 27.014303332957564 22.69768065688562 68-69 22.38763242831637 27.751754413796487 27.208700969434407 22.65191218845274 70-71 22.327178871352405 27.71678386055762 27.178366586208536 22.777670681881443 72-73 22.436570453931434 27.79946658513534 26.96838545858007 22.795577502353158 74-75 22.258044511618248 27.965614448008246 27.117993862057826 22.65834717831568 76-77 22.209336032462623 27.882603259241883 27.084401952333863 22.82365875596163 78-79 22.49500583964028 27.842236691593204 27.01247656752698 22.650280901239533 80-81 22.387803910728664 28.043265590635404 26.919105363731415 22.649825134904518 82-83 22.526709175726765 27.89527272587425 27.101689081350493 22.476329017048492 84-85 22.566038788008147 27.84717084443214 26.95832906687357 22.62846130068614 86-87 22.14811802253556 27.778859415463764 27.104133826944697 22.968888735055973 88-89 22.395784008556973 27.731705265182015 27.110387347218385 22.762123379042627 90-91 22.295124444550204 27.965441510328297 27.063982576537114 22.67545146858438 92-93 22.352034862409578 27.47210274123242 27.340287066848862 22.83557532950914 94-95 22.1969546378089 27.840602217260578 27.25812844516543 22.704314699765092 96-97 22.207031221832352 27.783762839640243 27.315293539642504 22.6939123988849 98-99 22.358177435253754 27.772496446819346 26.954465686478347 22.91486043144855 100-101 22.124610430160097 27.731940376284925 27.086467078073568 23.056982115481407 102-103 22.062878635647348 27.505112572746597 27.382285836803412 23.049722954802647 104-105 21.8502402558242 27.908686682416917 27.08387765761448 23.157195404144403 106-107 21.941479485034083 27.936812092627715 27.13599062387254 22.98571779846566 108-109 22.2248475424719 27.79499459058505 26.92319063422476 23.056967232718296 110-111 22.141817217733127 28.091133221018445 27.061166105452767 22.70588345579566 112-113 22.039625493227497 27.985444500849926 26.9152946673743 23.059635338548276 114-115 21.85028307660062 28.123685509756612 26.984769099197713 23.041262314445056 116-117 21.90620318133188 27.995427289113632 27.262360969662762 22.83600855989172 118-119 21.894637203805196 28.060137811812623 26.861862009835324 23.183362974546856 120-121 22.167488395168082 28.21671134254541 26.75274304767692 22.86305721460959 122-123 21.873423356402757 28.131564215529448 26.901472739854736 23.093539688213056 124-125 22.3393809713926 28.055116423903044 26.58834325996543 23.017159344738925 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 255.0 1 288.5 2 370.0 3 384.5 4 338.0 5 314.0 6 302.0 7 300.5 8 317.0 9 337.5 10 329.5 11 308.5 12 315.5 13 346.0 14 390.0 15 468.5 16 548.5 17 702.5 18 983.0 19 1287.0 20 1762.5 21 2558.5 22 3689.5 23 5046.5 24 6733.5 25 8952.0 26 11961.5 27 15636.0 28 19384.5 29 23044.0 30 27242.0 31 31561.0 32 36601.0 33 40160.5 34 42294.5 35 46309.5 36 51388.5 37 55391.5 38 58710.5 39 61317.5 40 63995.5 41 68890.5 42 72618.5 43 76697.0 44 80227.5 45 82348.0 46 82996.0 47 80228.5 48 77707.0 49 74646.5 50 72213.0 51 69226.5 52 66505.0 53 65202.0 54 63669.5 55 60810.5 56 54821.5 57 50466.5 58 45990.0 59 40418.5 60 34485.5 61 28003.0 62 23884.5 63 19730.0 64 15472.0 65 12050.0 66 8761.0 67 7303.0 68 5615.5 69 3451.5 70 2703.0 71 2276.0 72 1979.5 73 1613.5 74 1265.5 75 1097.0 76 729.5 77 448.0 78 331.0 79 251.0 80 171.5 81 101.5 82 71.0 83 48.5 84 36.5 85 31.5 86 22.0 87 11.5 88 9.0 89 9.5 90 9.0 91 6.5 92 4.0 93 4.0 94 4.5 95 3.5 96 3.0 97 2.0 98 1.0 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.8458062444355994E-4 2 1.4421773416633496E-4 3 9.614515611088998E-5 4 4.807257805544499E-4 5 0.0011537418733306797 6 7.691612488871199E-4 7 9.133789830534549E-4 8 0.0015383224977742397 9 0.0022594111686059146 10-11 0.002067120856384134 12-13 0.0025478466369385842 14-15 0.0035814070651306513 16-17 0.003052608706520757 18-19 0.002980499839437589 20-21 0.0036294796431860965 22-23 0.09362134576297912 24-25 0.002668028082077197 26-27 0.0020190482783286894 28-29 0.001898866833190077 30-31 0.0014662136306910721 32-33 0.00211519343443958 34-35 0.0012739233184692921 36-37 7.451249598593973E-4 38-39 0.0013219958965247371 40-41 0.0017786853880514648 42-43 0.001802721677079187 44-45 0.0017306128099960196 46-47 0.002187302301522747 48-49 4.5668949152672745E-4 50-51 0.0017786853880514648 52-53 0.0017065765209682972 54-55 0.0018267579661069098 56-57 0.0016104313648574074 58-59 8.653064049980098E-4 60-61 0.0012739233184692921 62-63 0.0017065765209682972 64-65 0.0012979596074970147 66-67 4.086169134712824E-4 68-69 2.884354683326699E-4 70-71 8.172338269425648E-4 72-73 2.884354683326699E-4 74-75 5.768709366653399E-4 76-77 5.28798358609895E-4 78-79 4.086169134712824E-4 80-81 6.249435147207849E-4 82-83 1.9229031222177997E-4 84-85 4.326532024990049E-4 86-87 4.326532024990049E-4 88-89 6.249435147207849E-4 90-91 2.1632660124950244E-4 92-93 5.047620695821723E-4 94-95 4.086169134712824E-4 96-97 4.326532024990049E-4 98-99 4.5668949152672745E-4 100-101 4.326532024990049E-4 102-103 0.00105759671721979 104-105 4.5668949152672745E-4 106-107 5.528346476376173E-4 108-109 8.893426940257324E-4 110-111 0.001177778162358402 112-113 5.768709366653399E-4 114-115 4.326532024990049E-4 116-117 0.001033560428192067 118-119 0.001562358786801962 120-121 9.614515611088998E-4 122-123 8.412701159702873E-4 124-125 4.5668949152672745E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2080188.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.276870128864623 #Duplication Level Percentage of deduplicated Percentage of total 1 48.02752631552046 13.100405979190796 2 13.551571310343894 7.392889013485964 3 10.021101371491909 8.20032841975116 4 7.34849848740943 8.017761555329006 5 5.26620771542516 7.182283196263847 6 3.66585081241572 5.999576191323388 7 2.5840200795820287 4.93387860847962 8 1.8669349410486706 4.07393135408193 9 1.359779929344245 3.338148648290372 >10 5.959821790710048 26.976389829896785 >50 0.23199903310723685 4.312572181531764 >100 0.1109884203015606 5.389551931384912 >500 0.0048092005967173305 0.8183684855739768 >1k 8.905927030958019E-4 0.26391460541663747 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.8072578055444985E-5 0.0 22-23 0.0 0.0 0.0 9.614515611088997E-5 0.0 24-25 0.0 0.0 0.0 2.643991793049474E-4 0.0 26-27 0.0 0.0 0.0 2.884354683326699E-4 0.0 28-29 0.0 0.0 0.0 4.8072578055444984E-4 0.0 30-31 0.0 0.0 0.0 0.0016585039429128522 0.0 32-33 0.0 0.0 0.0 0.005456237609293006 0.0 34-35 0.0 0.0 0.0 0.011777781623584022 0.0 36-37 0.0 0.0 0.0 0.022762365709253204 0.0 38-39 0.0 0.0 0.0 0.04350568314017771 0.0 40-41 0.0 0.0 0.0 0.06278278694041116 0.0 42-43 0.0 0.0 0.0 0.08854968877812967 0.0 44-45 0.0 0.0 0.0 0.1213832595899986 0.0 46-47 0.0 0.0 0.0 0.16106717277476845 0.0 48-49 0.0 0.0 0.0 0.20351525919772634 0.0 50-51 0.0 0.0 0.0 0.24745359554040308 0.0 52-53 0.0 0.0 0.0 0.2981701653888975 0.0 54-55 0.0 0.0 0.0 0.3595348112766731 0.0 56-57 0.0 0.0 0.0 0.42986499297178904 0.0 58-59 0.0 0.0 0.0 0.510939395862297 0.0 60-61 0.0 0.0 0.0 0.5937203752737733 0.0 62-63 0.0 0.0 0.0 0.6823902454970416 0.0 64-65 0.0 0.0 0.0 0.7864193044090246 0.0 66-67 0.0 0.0 0.0 0.9040288666216707 0.0 68-69 0.0 0.0 0.0 1.0407953511894117 0.0 70-71 0.0 0.0 0.0 1.1917192099944813 0.0 72-73 0.0 0.0 0.0 1.349397266016341 0.0 74-75 0.0 0.0 0.0 1.5198626278009488 0.0 76-77 0.0 0.0 0.0 1.7220558911021504 0.0 78-79 0.0 0.0 0.0 1.9525639028780093 0.0 80-81 0.0 0.0 0.0 2.201748111228408 0.0 82-83 0.0 0.0 0.0 2.4653781292844688 0.0 84-85 0.0 0.0 0.0 2.7468911463771546 0.0 86-87 0.0 0.0 0.0 3.06323274627101 0.0 88-89 0.0 0.0 0.0 3.4080333123736892 0.0 90-91 0.0 0.0 0.0 3.7666066720892535 0.0 92-93 0.0 0.0 0.0 4.1471011273981 0.0 94-95 0.0 0.0 0.0 4.549877222635646 0.0 96-97 0.0 0.0 0.0 4.997096416285451 0.0 98-99 0.0 0.0 0.0 5.480562333789061 0.0 100-101 0.0 0.0 0.0 5.997847309954677 0.0 102-103 0.0 0.0 0.0 6.517680132757231 0.0 104-105 0.0 0.0 0.0 7.051045386282394 0.0 106-107 0.0 0.0 0.0 7.628108613259956 0.0 108-109 0.0 0.0 0.0 8.248220833886169 0.0 110-111 0.0 0.0 0.0 8.86335754268364 0.0 112-113 0.0 0.0 0.0 9.484286997136799 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2555 0.0 56.5824 1 GTATCAA 2920 0.0 48.0834 2 GTACATG 9250 0.0 47.787365 1 TACATGG 9330 0.0 46.16607 2 ACATGGG 9230 0.0 45.1193 3 ATCAACG 3375 0.0 42.482418 4 TCAACGC 3375 0.0 42.306145 5 CATGGGG 5640 0.0 40.400402 4 CAACGCA 3520 0.0 40.394405 6 AACGCAG 3685 0.0 38.42425 7 TATCAAC 4085 0.0 34.516144 3 CTAACGT 70 0.0027911083 33.99601 4 ACGCAGA 4390 0.0 31.71154 8 CGCAGAG 4415 0.0 31.531975 9 ATGGGGA 3605 0.0 30.695427 5 CATGGGA 4070 0.0 29.96577 4 ATGGGGG 3090 0.0 28.880104 5 GAGTACA 6170 0.0 26.130644 1 AGTACAT 6265 0.0 24.879763 2 CATGGGC 2250 0.0 22.210726 4 >>END_MODULE