FastQCFastQC Report
Thu 26 May 2016
SRR936202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080188
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.24690076089276544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.17200368428238216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35050.16849438608433467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.079.183681
GTATCAA59050.055.345682
TCAACGC79350.042.7621155
ATCAACG79000.042.725514
GTACATG78150.042.684721
CAACGCA81200.041.8350036
AACGCAG84400.040.2488487
TACATGG82000.040.073432
ACATGGG81200.039.9550553
TATCAAC85700.039.732553
ACGCAGA96300.034.7192088
CGCAGAG97200.034.2141159
CATGGGA36150.031.7819294
CATGGGG49850.031.2872494
GTGGTAT12050.029.6604391
CAGAGTA108750.025.8756719
GCAGAGT110650.025.5926518
GAGTACA56050.024.4436241
AGAGTAC106100.023.85854310-11
ATGGGGG27850.023.7262465
Produced by FastQC (version 0.11.5)