FastQCFastQC Report
Thu 26 May 2016
SRR936201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348074
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG113040.2599771761014187No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG91600.21066798771134068No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA79120.18196562432010127No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA77710.17872280922541797No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA74530.17140922624591945No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA60820.13987802415506267No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA59800.13753215791635562No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG57920.13320840445677787No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA54100.12442290540593376No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA53150.12223802998753011No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA49840.11462546405603953No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG46860.10777185484883653No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT44620.10262014859912687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.056.1535071
TACATGG185200.051.6238252
GTACATG191550.050.968481
ACATGGG190700.049.1990053
GTATCAA55100.041.67842
CATGGGG114750.040.647954
CAACGCA59800.038.1042026
CATGGGA78550.037.946074
ATCAACG60150.037.7835734
TCAACGC61500.037.0509155
AACGCAG63250.036.5901647
ATGGGGG60600.032.201535
GAGTACA111850.031.5955371
TATCAAC74750.031.199683
ACGCAGA76350.028.9873948
CGCAGAG78650.027.4589049
CATGGGT36600.024.8705654
ATGGGGA57350.024.689875
ATGGGGC42000.023.514415
ATGGGAG43200.023.136675