FastQCFastQC Report
Thu 26 May 2016
SRR936201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348074
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG116740.2684866908888855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89060.2048263208031878No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG78750.18111467284135457No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA68470.1574720209453657No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA67140.1544131953596006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66910.15388422552146075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.1521133264981231No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA64260.14778957303854534No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG59180.13610623922223955No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA55320.12722874541693632No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA52590.12095010342510269No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA52040.11968517555129006No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA50560.11628136963630334No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT49010.11271657290101318No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA46900.10786384960329563No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT44010.1012172285936256No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG43890.1009412443302483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.078.1021651
GTATCAA111600.049.9304282
TACATGG172450.042.2198682
GTACATG176950.041.9123841
ACATGGG174950.041.07213
CAACGCA148650.038.7822276
ATCAACG149850.038.416994
TCAACGC150950.038.137045
AACGCAG153800.037.5222857
TATCAAC159100.036.557633
CATGGGG105700.033.8495674
ACGCAGA182800.031.276718
CGCAGAG185850.030.507339
CATGGGA74000.028.5594774
GAGTACA115050.028.4255751
ATGGGGG56400.027.0218145
CAGAGTA213250.025.4157649
GCAGAGT224550.024.0307928
AGAGTAC205450.023.6731210-11
CATGGGT34600.023.2279724
Produced by FastQC (version 0.11.5)