FastQCFastQC Report
Thu 26 May 2016
SRR936200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292198
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42371.45004414814612No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24380.8343657383007413No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12880.4407969938192595No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11810.40417798889793904No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.23443007823462175No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6080.20807808403890513No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5930.20294457867610316No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG4750.16256100315539462No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.13586677526882457No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3860.13210220466943648No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3830.1310755035968761No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.1245730634706603No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT3360.11499052012676335No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3340.11430605274505644No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3170.10848808000054758No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.10814584630969412No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3020.10335457463774564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA5950.062.9904861
CATGGGT2850.060.5347184
GGATCGC300.004235926559.4910168
CACGTCG150.004238044459.49101636-37
GGTATCA7100.058.653111
GGGTATG1103.6379788E-1254.082747
CAGTGCG551.3094492E-554.082749
ATGGGTA1950.051.863965
GTACATG25700.051.852091
GAGAACA2900.051.285361
GGTATGG856.187656E-848.99268
GGTACCT1004.134563E-947.592818
ACATGGG27500.046.2948273
TACATGG28900.045.698982
GTATCAA9050.045.3577882
TGGGAAC2600.043.4742056
CATGGGG7400.043.4123654
ATATAGG1650.043.2661932
TGGGTAC1109.602445E-943.266196
GCTTACT1700.041.9936568
Produced by FastQC (version 0.11.5)