##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936200_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 292198 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.478028597047206 31.0 28.0 33.0 10.0 34.0 2 27.868575418038454 31.0 28.0 34.0 12.0 34.0 3 28.129538874324943 31.0 28.0 34.0 15.0 34.0 4 31.00127995400379 35.0 32.0 37.0 10.0 37.0 5 32.03389140240522 35.0 32.0 37.0 19.0 37.0 6 31.53306661921026 35.0 32.0 37.0 13.0 37.0 7 31.77264046981841 35.0 32.0 37.0 15.0 37.0 8 31.427374588463987 35.0 32.0 37.0 13.0 37.0 9 32.42758677335232 38.0 32.0 39.0 10.0 39.0 10-11 32.59872928630586 38.0 32.0 39.0 10.0 39.0 12-13 32.65701168385821 38.0 32.0 39.0 11.0 39.0 14-15 33.69677410523002 39.0 32.0 41.0 11.0 41.0 16-17 33.81009794728232 39.0 32.0 41.0 12.5 41.0 18-19 33.76823763338558 39.0 32.0 41.0 12.5 41.0 20-21 33.80163621927597 39.0 32.0 41.0 13.0 41.0 22-23 33.54774673337943 39.0 32.0 41.0 11.5 41.0 24-25 33.418207859054476 39.0 31.5 41.0 10.0 41.0 26-27 33.38615767390605 39.0 32.0 41.0 8.0 41.0 28-29 33.18324731859903 39.0 31.0 41.0 2.0 41.0 30-31 33.05737376710313 38.0 31.0 41.0 2.0 41.0 32-33 32.851811100692 38.0 31.0 40.5 2.0 41.0 34-35 32.759064059302254 38.0 30.5 40.0 2.0 41.0 36-37 32.60807911073998 38.0 30.0 40.0 2.0 41.0 38-39 32.33261179063512 38.0 30.0 40.0 2.0 41.0 40-41 32.378974188735036 38.0 30.0 40.0 2.0 41.0 42-43 32.25175737000254 38.0 30.0 40.0 2.0 41.0 44-45 32.14547156380263 38.0 30.0 40.0 2.0 41.0 46-47 32.1138252144094 38.0 30.0 40.0 2.0 41.0 48-49 31.99781826022081 38.0 30.0 40.0 2.0 41.0 50-51 31.865112013087018 38.0 30.0 40.0 2.0 41.0 52-53 31.70302671476191 37.0 30.0 40.0 2.0 41.0 54-55 31.548828191842517 37.0 30.0 40.0 2.0 41.0 56-57 31.350260782072432 37.0 30.0 40.0 2.0 41.0 58-59 31.11568696568765 36.0 30.0 40.0 2.0 41.0 60-61 30.885615233506044 36.0 29.5 40.0 2.0 41.0 62-63 30.64044072854708 35.5 29.0 39.5 2.0 41.0 64-65 30.355164990862363 35.0 29.0 39.0 2.0 41.0 66-67 30.06757917576438 35.0 29.0 39.0 2.0 41.0 68-69 29.78786986906139 35.0 29.0 39.0 2.0 40.0 70-71 29.46937179583707 35.0 28.0 38.0 2.0 40.0 72-73 29.145030082341428 35.0 28.0 37.0 2.0 40.0 74-75 28.823696602988385 34.0 28.0 37.0 2.0 39.0 76-77 28.54475903325827 34.0 28.0 36.0 2.0 39.0 78-79 28.244368544617007 34.0 27.0 36.0 2.0 38.5 80-81 27.982852380919788 34.0 27.0 35.5 2.0 37.5 82-83 27.71688546807302 34.0 26.5 35.0 2.0 37.0 84-85 27.715603802900773 34.0 27.0 35.0 2.0 37.0 86-87 27.609898082806865 34.0 27.0 35.0 2.0 36.0 88-89 27.448247421269137 34.0 27.0 35.0 2.0 36.0 90-91 27.28299303896673 34.0 27.0 35.0 2.0 36.0 92-93 27.1658909369674 34.0 26.5 35.0 2.0 35.5 94-95 27.03086776774653 34.0 26.0 35.0 2.0 35.0 96-97 26.928769190754213 34.0 26.0 35.0 2.0 35.0 98-99 26.832433829115875 34.0 25.5 35.0 2.0 35.0 100-101 26.735870882073115 34.0 25.0 35.0 2.0 35.0 102-103 25.851561270097676 32.5 24.5 34.5 2.0 35.0 104-105 26.412513090438676 33.5 25.0 35.0 2.0 35.0 106-107 26.522749984599486 34.0 25.0 35.0 2.0 35.0 108-109 26.466567190740527 34.0 25.0 35.0 2.0 35.0 110-111 26.340760374814337 34.0 25.0 35.0 2.0 35.0 112-113 26.3029521078173 34.0 25.0 35.0 2.0 35.0 114-115 26.172184614542193 34.0 24.0 35.0 2.0 35.0 116-117 26.042466067529553 34.0 24.0 35.0 2.0 35.0 118-119 25.84311494260741 34.0 23.0 35.0 2.0 35.0 120-121 25.644600579059407 34.0 21.5 35.0 2.0 35.0 122-123 25.360238605329265 34.0 17.0 35.0 2.0 35.0 124-125 23.53317100048597 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08661783958532254 1101 2 0.1549620349905929 1101 3 0.14950137247665296 1101 4 0.1905169821327206 1101 5 0.3944752975618684 1101 6 0.10662111467603452 1101 7 0.2198866379052653 1101 8 0.4738290865861394 1101 9 0.3396355014737722 1101 10-11 0.07395403241815046 1101 12-13 0.11620365796031251 1101 14-15 0.23626937794151814 1101 16-17 0.10778517638438956 1101 18-19 0.16470852607486108 1101 20-21 0.11515038111916454 1101 22-23 0.10343473616558896 1101 24-25 0.42198875571997974 1101 26-27 0.40672283510907903 1101 28-29 0.36552353082345945 1101 30-31 0.5074181972034211 1101 32-33 0.4513318443613201 1101 34-35 0.34059974858305964 1101 36-37 0.20434619065348159 1101 38-39 0.43771853985280273 1101 40-41 0.32450697463848144 1101 42-43 0.17388160372574646 1101 44-45 0.2615038739071238 1101 46-47 0.26471510475235505 1101 48-49 0.4450676328689269 1101 50-51 0.4335022998143465 1101 52-53 0.18765316902253915 1101 54-55 0.16910450213509876 1101 56-57 0.1238040636776141 1101 58-59 0.1013634441859601 1101 60-61 0.060934054283269035 1101 62-63 0.08492598427327991 1101 64-65 0.14549475742004603 1101 66-67 0.1860828237742318 1101 68-69 0.23103222621785235 1101 70-71 0.31297858939149137 1101 72-73 0.07285170667641694 1101 74-75 0.11151208713889105 1101 76-77 0.16838519852295164 1101 78-79 0.1906655082262425 1101 80-81 -0.035867211507557784 1101 82-83 0.1936278293402438 1101 84-85 0.09730130265691272 1101 86-87 0.05649584762414861 1101 88-89 -0.02779258569220744 1101 90-91 -0.1529480117372337 1101 92-93 -0.008292034084146138 1101 94-95 0.02509320837338791 1101 96-97 -0.16250590803736742 1101 98-99 -0.04782332014972823 1101 100-101 0.07898686687915912 1101 102-103 0.18845437836558077 1101 104-105 0.02303279088873822 1101 106-107 -0.0050436921468914875 1101 108-109 0.016908378779817212 1101 110-111 -0.06839735121151591 1101 112-113 -0.07264821530642251 1101 114-115 -0.04593055002115776 1101 116-117 -0.11755776256756079 1101 118-119 -0.2135695648306104 1101 120-121 -0.030514165748147803 1101 122-123 0.1003251524140687 1101 124-125 -0.19816676593848115 1102 1 0.8863332844917338 1102 2 0.7345947276560096 1102 3 0.6794374732674733 1102 4 0.9547887272196185 1102 5 0.6346025932327066 1102 6 1.0788493419380245 1102 7 0.6686290149960676 1102 8 0.6963049169993489 1102 9 1.002553347502321 1102 10-11 0.9402407895458467 1102 12-13 0.8620094868149977 1102 14-15 1.2346128248790293 1102 16-17 1.1187852920163195 1102 18-19 1.114144694736595 1102 20-21 1.236666969273422 1102 22-23 1.2574338412750095 1102 24-25 1.6488611131533517 1102 26-27 1.5220279777134067 1102 28-29 1.568522922498964 1102 30-31 1.5387293124482397 1102 32-33 1.5829938773717274 1102 34-35 1.2463748092898186 1102 36-37 1.4221892027143923 1102 38-39 1.706502443888354 1102 40-41 1.2346886173157188 1102 42-43 1.4856102005071605 1102 44-45 1.4162017980626302 1102 46-47 1.5603582947858854 1102 48-49 1.2998188482610402 1102 50-51 1.268980689212409 1102 52-53 1.1101697219844766 1102 54-55 1.122700906484873 1102 56-57 1.3627574439005308 1102 58-59 1.2769103077955855 1102 60-61 1.2756298668992123 1102 62-63 1.2311727226987763 1102 64-65 1.1264557090205436 1102 66-67 1.1563377670391723 1102 68-69 1.3199657479139226 1102 70-71 1.2192161778122106 1102 72-73 1.1391615901898149 1102 74-75 1.224014412346147 1102 76-77 1.0600581914291816 1102 78-79 1.074342301903691 1102 80-81 0.9855925661774876 1102 82-83 1.0593515092509556 1102 84-85 1.151283817098335 1102 86-87 1.096788547227483 1102 88-89 1.20411981558075 1102 90-91 1.2720596551623693 1102 92-93 1.1023978863309196 1102 94-95 1.1992600300538747 1102 96-97 1.1702953508424123 1102 98-99 0.868647693942286 1102 100-101 0.6906345213106277 1102 102-103 1.0186891111804144 1102 104-105 0.9629142430246667 1102 106-107 1.212012520052781 1102 108-109 1.1677225783806335 1102 110-111 0.9770384554720053 1102 112-113 0.97980091921373 1102 114-115 0.8333296067355178 1102 116-117 0.9603178275323359 1102 118-119 1.100393744655733 1102 120-121 1.1634533668173255 1102 122-123 1.2029384339979252 1102 124-125 1.338898294617458 1103 1 -0.2987789306872948 1103 2 -0.3602171049063827 1103 3 0.022198214135119798 1103 4 -0.22331836736854527 1103 5 -0.30480216187360654 1103 6 -0.012231502483579959 1103 7 -0.4684562456972081 1103 8 -0.3262808862100641 1103 9 -0.22295252499687024 1103 10-11 -0.02092885620063356 1103 12-13 0.014946688193177238 1103 14-15 0.10909886222697907 1103 16-17 -0.07865488844167601 1103 18-19 0.03042113050078399 1103 20-21 0.054795574150361404 1103 22-23 0.09564981980279441 1103 24-25 0.012851366898622985 1103 26-27 -0.20285685658122077 1103 28-29 -0.09399635110049331 1103 30-31 -0.1836547128311139 1103 32-33 -0.045318326385917373 1103 34-35 -0.017190473484816948 1103 36-37 -0.025786355506156156 1103 38-39 0.2269277003937944 1103 40-41 0.15972383327520845 1103 42-43 0.5165288611585623 1103 44-45 0.5058002409461508 1103 46-47 0.2690223726036436 1103 48-49 -0.014126671697837878 1103 50-51 0.07799835429911539 1103 52-53 0.21730000199894306 1103 54-55 0.17626961567718524 1103 56-57 -0.061825675281696135 1103 58-59 -0.06850352731758491 1103 60-61 -0.05428656658069286 1103 62-63 -0.02863539791112757 1103 64-65 -0.40105556960432054 1103 66-67 -0.2182577253180149 1103 68-69 -0.17039983181691198 1103 70-71 -0.23455811644092606 1103 72-73 -0.2779928651049488 1103 74-75 -0.3541792944667108 1103 76-77 -0.1207781400196204 1103 78-79 -0.01952964525333556 1103 80-81 -0.2184220532046126 1103 82-83 -0.21903302999104568 1103 84-85 -0.08736617433561733 1103 86-87 -0.18895089274358412 1103 88-89 -0.0886128476975685 1103 90-91 -0.043720425929514306 1103 92-93 0.04209769407081865 1103 94-95 -0.0632178409986075 1103 96-97 -0.03031426596987785 1103 98-99 -0.2451569621786298 1103 100-101 -0.17181622393521678 1103 102-103 -0.2421421758201383 1103 104-105 -0.1553257204268803 1103 106-107 -0.23529441082876446 1103 108-109 -0.3039999579298751 1103 110-111 -0.23986568097556216 1103 112-113 -0.35710635811670954 1103 114-115 -0.13257753734534816 1103 116-117 -0.1915771718014163 1103 118-119 -0.2784776314316808 1103 120-121 -0.3016019317569061 1103 122-123 -0.5327467543598701 1103 124-125 -0.2887809736520097 1104 1 -0.19003993749905845 1104 2 0.3699014086894543 1104 3 0.6400795528587899 1104 4 0.3154846822336417 1104 5 0.09942242323650419 1104 6 0.7763869465020434 1104 7 -0.030039011383646397 1104 8 0.2613859465971551 1104 9 0.6423720951873619 1104 10-11 0.37714160585471035 1104 12-13 0.5500900305719512 1104 14-15 0.7744710170699776 1104 16-17 0.6635018928619729 1104 18-19 0.6265288265004898 1104 20-21 0.14582675319449834 1104 22-23 0.10232313209166222 1104 24-25 0.06501027110935809 1104 26-27 0.021352129776794015 1104 28-29 0.10236101723311464 1104 30-31 0.09891345094655435 1104 32-33 -0.09822717060145436 1104 34-35 0.08928946965819051 1104 36-37 0.11047658961208384 1104 38-39 0.2158608934306585 1104 40-41 -0.13034142304275065 1104 42-43 0.24923178338632113 1104 44-45 0.1489003314004549 1104 46-47 0.03860849802130417 1104 48-49 -0.06081216762739672 1104 50-51 -0.006887931721081486 1104 52-53 0.11357060804276742 1104 54-55 0.20880778185465942 1104 56-57 0.21732506958179343 1104 58-59 0.13786842446473813 1104 60-61 0.11527832976605978 1104 62-63 0.27257742813970864 1104 64-65 0.17502295949796576 1104 66-67 0.09281046614974642 1104 68-69 0.16214202637289432 1104 70-71 0.29030527910281734 1104 72-73 0.3152789039227599 1104 74-75 0.23363983384917475 1104 76-77 0.25701855932135587 1104 78-79 0.05654657634124405 1104 80-81 0.11629578475296398 1104 82-83 -0.11371607073240142 1104 84-85 -0.07665010854396215 1104 86-87 0.017197230791147433 1104 88-89 0.012010640884884793 1104 90-91 0.016803193719375997 1104 92-93 -0.055449886738102805 1104 94-95 0.11221273993953318 1104 96-97 -0.007878368533980051 1104 98-99 -0.05145553521586166 1104 100-101 -0.05902755472106236 1104 102-103 0.057967661546719285 1104 104-105 0.21314634026980173 1104 106-107 0.3517470873898425 1104 108-109 0.20276565466726737 1104 110-111 0.1508299864479703 1104 112-113 0.32259772709493717 1104 114-115 0.42590393798278114 1104 116-117 0.4901898253003516 1104 118-119 0.46223695755504934 1104 120-121 0.31679628869315124 1104 122-123 0.1566488632214913 1104 124-125 0.2564207948218957 1105 1 0.18091733870854299 1105 2 0.35055102538964533 1105 3 0.2527381796536261 1105 4 0.17965657852485606 1105 5 0.22880812388260097 1105 6 0.5226443274566321 1105 7 0.19204777102998705 1105 8 -0.10646136381543414 1105 9 0.22695760302484302 1105 10-11 0.37246738751749575 1105 12-13 0.28105945001409793 1105 14-15 0.3952081392160878 1105 16-17 0.03861428730389349 1105 18-19 0.19522616333156861 1105 20-21 0.1306732015301293 1105 22-23 0.05044788296995506 1105 24-25 0.7007479957823719 1105 26-27 0.5225154701859509 1105 28-29 0.6589336588037682 1105 30-31 0.6370140080521978 1105 32-33 0.6742123217928011 1105 34-35 0.6431530575668631 1105 36-37 0.8496125660301033 1105 38-39 0.7427011496436364 1105 40-41 0.6120412464295342 1105 42-43 0.7444185203786873 1105 44-45 0.7729722082254185 1105 46-47 0.7420424378983839 1105 48-49 0.818821496014273 1105 50-51 1.0438332466361508 1105 52-53 0.9136290068043422 1105 54-55 0.9812611787159611 1105 56-57 1.054480586410829 1105 58-59 1.1047155402147872 1105 60-61 0.8626735596069004 1105 62-63 0.6717607724421235 1105 64-65 0.5572915196440569 1105 66-67 0.49485349584042027 1105 68-69 0.685580322982986 1105 70-71 0.569314632174315 1105 72-73 0.6273160010408247 1105 74-75 0.7275033290614026 1105 76-77 0.9950329087313428 1105 78-79 0.6542671707108632 1105 80-81 0.48241430643003014 1105 82-83 0.6803332808559546 1105 84-85 0.7171072492365163 1105 86-87 0.8850375889702207 1105 88-89 0.9475147705115923 1105 90-91 0.7998610641112052 1105 92-93 0.7986088271913943 1105 94-95 0.6462022096231586 1105 96-97 0.6122933052857604 1105 98-99 0.6513527036443421 1105 100-101 0.6785881999670593 1105 102-103 0.8766997595454029 1105 104-105 0.8020584547684528 1105 106-107 0.9400705794503921 1105 108-109 0.7763712683640946 1105 110-111 0.382144700154992 1105 112-113 0.35980608089873556 1105 114-115 0.455900508214242 1105 116-117 0.6965413858450411 1105 118-119 0.8897205271098301 1105 120-121 0.7132636271000727 1105 122-123 0.7867297872196772 1105 124-125 0.7236039351921235 1106 1 -0.10647123837960493 1106 2 0.21456921987994448 1106 3 0.33630077823768545 1106 4 0.19762180357162862 1106 5 0.479385872314424 1106 6 0.3894778992258239 1106 7 -0.05819983544079932 1106 8 0.09038578045660017 1106 9 -0.22936278140712574 1106 10-11 0.1831309728591961 1106 12-13 -0.09295929471279862 1106 14-15 -0.06825156512343966 1106 16-17 -0.12138993117672214 1106 18-19 -0.1715019464222891 1106 20-21 -0.060589041234251795 1106 22-23 -0.2440937699407968 1106 24-25 -3.70296059891335 1106 26-27 -0.9881132668376296 1106 28-29 -0.7606630177671576 1106 30-31 -0.9208342000106029 1106 32-33 -5.3893354204030075 1106 34-35 -1.3259511572455054 1106 36-37 -0.6796325093523095 1106 38-39 -0.30726033379423967 1106 40-41 -0.6287377051863317 1106 42-43 -0.33496686533717224 1106 44-45 -0.5123621522162409 1106 46-47 -0.5194391658578965 1106 48-49 -0.3966053041764752 1106 50-51 -0.4155913892906291 1106 52-53 -0.20898204928310804 1106 54-55 -0.4572773928698233 1106 56-57 -0.2958000342560574 1106 58-59 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-2.376800144432501 2216 98-99 -2.308970409713009 2216 100-101 -2.252622046020111 2216 102-103 -2.1912983168367433 2216 104-105 -2.307762431003127 2216 106-107 -2.205273720924353 2216 108-109 -2.155231413812494 2216 110-111 -2.141328881857362 2216 112-113 -2.3860767417382576 2216 114-115 -2.340985408543915 2216 116-117 -2.3716154797467013 2216 118-119 -2.433764850672386 2216 120-121 -2.34138708200717 2216 122-123 -2.496150921252408 2216 124-125 -2.424993275561988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14259.0 3 2743.0 4 3832.0 5 4826.0 6 4865.0 7 2299.0 8 1559.0 9 1330.0 10 1484.0 11 1748.0 12 1887.0 13 1894.0 14 1869.0 15 1747.0 16 1499.0 17 1363.0 18 1107.0 19 1161.0 20 1062.0 21 1229.0 22 1409.0 23 2583.0 24 3735.0 25 4098.0 26 5811.0 27 4268.0 28 4845.0 29 5622.0 30 6477.0 31 7909.0 32 10134.0 33 13463.0 34 18535.0 35 27371.0 36 43991.0 37 58420.0 38 19747.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.89353721474627 22.322003039055975 9.162000848745363 20.622458897452393 2 18.49259748526684 27.116202027392383 32.55840217934414 21.832798307996633 3 15.30195278544001 35.741517737972195 26.88040301439435 22.076126462193443 4 9.85667253027057 21.65072998446259 33.059774536444465 35.43282294882238 5 10.187305140025394 47.89782235957248 30.640971673220463 11.273900827181661 6 27.378882667798326 42.44240167558762 16.676135196922615 13.50258045969144 7 25.849252903852864 40.459893633768885 19.606225915304005 14.084627547074243 8 25.566997607778312 38.255697354866754 18.06520943062386 18.112095606731074 9 25.64221031232674 21.085306337570245 16.406223262626884 36.86626008747613 10-11 21.320558737416345 33.95341116961476 25.85093148570558 18.875098607263315 12-13 22.19286567440014 33.57130389845068 24.87414140653609 19.36168902061309 14-15 21.17916222464454 34.50060833599997 21.79981895373053 22.52041048562496 16-17 20.86727962503786 35.90033180521545 22.940420514768668 20.291968054978017 18-19 19.91437107919135 34.70538106923211 24.528305115489527 20.85194273608701 20-21 21.912529219101334 33.83323807535534 24.328773499344603 19.925459206198727 22-23 21.417792979889935 33.64877525747769 24.32391232484409 20.609519437788286 24-25 21.148765195487897 34.07468376957617 24.39149600262841 20.385055032307523 26-27 20.521881085446946 34.085689937815076 24.711064262319766 20.68136471441821 28-29 20.624582898406885 33.13437943838874 25.391947158575608 20.84909050462876 30-31 20.504352379306393 33.793525932035465 24.87093445677823 20.831187231879916 32-33 20.79098133431898 34.18037331362041 24.601463411295235 20.427181940765383 34-35 20.817633309034658 33.77088060452366 24.83410165059874 20.57738443584294 36-37 20.460476218995712 34.02612958700878 24.193225472497197 21.320168721498302 38-39 20.236655002181745 34.39197802855945 24.853053157538994 20.518313811719814 40-41 20.012696913383778 34.09771489782578 25.661625547147988 20.227962641642453 42-43 20.472217278812852 34.38240085422114 24.411696258675683 20.733685608290326 44-45 20.252945786575495 34.17728450326321 24.62157380123684 20.948195908924458 46-47 20.526426565684393 33.87121337866812 24.889928524126745 20.71243153152074 48-49 20.32885235354109 34.42580031348606 24.776008049336408 20.46933928363644 50-51 20.50866541636436 34.22291886268121 24.337259921422607 20.93115579953182 52-53 20.443916637719052 34.0379439038038 24.672435655017463 20.84570380345968 54-55 20.82814275349423 34.263644950649564 24.50957576421306 20.398636531643145 56-57 20.683376911388248 34.3839409163712 24.244171720352092 20.688510451888458 58-59 20.66701460484775 33.35843051360809 24.892581216471736 21.081973665072425 60-61 21.033754624448576 34.49487845528873 24.202164977737624 20.26920194252508 62-63 19.7023573217977 34.67963284918342 24.92299689249682 20.695012936522062 64-65 20.562190169971235 34.036855335365075 24.716757387579932 20.68419710708376 66-67 20.668861525404004 34.564918308817994 24.16238304163615 20.60383712414185 68-69 20.381385225087097 34.64106530503289 24.57391905488744 20.403630414992573 70-71 20.81417534373155 33.823869129612675 24.700930021646318 20.66102550500945 72-73 20.61324855064032 34.717896768629494 23.90690559141404 20.761949089316147 74-75 20.73576821196586 34.90150514377237 24.163409742708712 20.199316901553058 76-77 20.311227318462137 34.13062375512495 25.160507601010274 20.39764132540264 78-79 20.54822414937816 34.024702427805806 24.6639265155819 20.763146907234137 80-81 20.331247989377065 34.63404951437039 24.30184327065894 20.732859225593607 82-83 21.688033456765616 33.509982956762194 24.02480509791306 20.777178488559127 84-85 20.362049021553876 33.43965393329181 24.516766028514912 21.6815310166394 86-87 20.598874735624474 33.755706746794985 24.761805351165993 20.883613166414555 88-89 20.85863010698225 33.24440961265991 24.899212178043655 20.997748102314183 90-91 20.55763557587663 33.548142013292356 24.965434397223802 20.92878801360721 92-93 20.74877309221829 33.73123703789896 24.35625842750464 21.163731442378115 94-95 20.460441207674247 33.24150062628766 24.87850703974702 21.419551126291076 96-97 20.928616896761785 33.558580140863384 24.802017809841274 20.710785152533557 98-99 20.39490345587581 34.059268030582004 24.86019753728636 20.685630976255826 100-101 19.838602591393506 33.93007481228482 24.953627334889354 21.277695261432317 102-103 20.79723474704609 33.61579068951651 24.55804721121844 21.02892735221896 104-105 19.34544384287367 35.09349824434116 24.63569223608649 20.925365676698675 106-107 20.05198529764064 34.51255655411741 24.66478209980903 20.77067604843291 108-109 20.32974272538266 34.992256949494774 23.601331291335484 21.07666903378708 110-111 19.82895072322906 35.47082186131593 23.722392294226662 20.977835121228352 112-113 19.716767397449676 34.87138857897727 24.86242205627691 20.54942196729615 114-115 19.596814488805535 35.42563604131445 24.1671743133081 20.810375156571915 116-117 19.83559037224886 35.42524392789796 24.17239054473523 20.56677515511795 118-119 19.755917261016577 34.68202850141686 24.258545633752686 21.303508603813878 120-121 19.679497600082136 35.08483132128099 24.26509467055673 20.970576408080152 122-123 19.78507687437435 35.03058718845986 24.31095406360424 20.87338187356155 124-125 19.958042149501367 35.309961053805985 23.649716972737664 21.082279823954988 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3307.0 1 4267.5 2 4835.0 3 3705.5 4 2327.5 5 1425.5 6 1012.5 7 836.0 8 775.5 9 743.0 10 613.5 11 441.5 12 390.0 13 365.5 14 324.0 15 312.5 16 342.0 17 350.0 18 371.0 19 404.0 20 448.0 21 542.0 22 641.5 23 775.0 24 884.5 25 1012.5 26 1249.5 27 1500.5 28 1745.5 29 2257.5 30 3186.5 31 3902.5 32 4844.0 33 6350.5 34 7644.5 35 8480.5 36 10238.5 37 11910.5 38 11208.0 39 10618.0 40 10122.0 41 9032.5 42 7959.5 43 7848.0 44 8511.0 45 8687.0 46 7933.0 47 6952.0 48 6649.5 49 6710.0 50 7225.0 51 6867.0 52 6809.5 53 7038.0 54 6890.5 55 6934.5 56 6465.5 57 6111.0 58 6139.5 59 5715.5 60 4950.5 61 4473.5 62 4095.0 63 3358.0 64 2673.5 65 2270.0 66 1781.0 67 1436.5 68 1109.0 69 722.0 70 618.0 71 591.0 72 562.5 73 501.5 74 415.0 75 353.0 76 254.5 77 172.5 78 111.5 79 72.0 80 48.0 81 22.5 82 16.5 83 13.5 84 11.0 85 8.5 86 5.5 87 3.5 88 3.5 89 1.5 90 2.0 91 2.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.844673817069248E-4 2 0.0 3 0.0 4 0.0 5 3.422336908534624E-4 6 6.844673817069248E-4 7 0.0 8 3.422336908534624E-4 9 0.0013689347634138495 10-11 0.0011978179179871183 12-13 0.0017111684542673118 14-15 0.002566752681400968 16-17 0.00290898637225443 18-19 0.0023956358359742366 20-21 0.0030801032176811616 22-23 0.09565431659354273 24-25 0.0020534021451207745 26-27 0.0017111684542673118 28-29 0.0010267010725603873 30-31 5.133505362801936E-4 32-33 0.0013689347634138495 34-35 3.422336908534624E-4 36-37 1.711168454267312E-4 38-39 1.711168454267312E-4 40-41 3.422336908534624E-4 42-43 6.844673817069248E-4 44-45 3.422336908534624E-4 46-47 5.133505362801936E-4 48-49 0.0 50-51 6.844673817069248E-4 52-53 0.0011978179179871183 54-55 6.844673817069248E-4 56-57 6.844673817069248E-4 58-59 1.711168454267312E-4 60-61 3.422336908534624E-4 62-63 6.844673817069248E-4 64-65 5.133505362801936E-4 66-67 0.0 68-69 0.0 70-71 1.711168454267312E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 1.711168454267312E-4 104-105 0.0 106-107 0.0 108-109 1.711168454267312E-4 110-111 5.133505362801936E-4 112-113 0.0 114-115 0.0 116-117 3.422336908534624E-4 118-119 6.844673817069248E-4 120-121 1.711168454267312E-4 122-123 1.711168454267312E-4 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 292198.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.954003791949297 #Duplication Level Percentage of deduplicated Percentage of total 1 63.55212796343902 19.03640682003299 2 9.977720651242503 5.977453644446574 3 6.706655241359612 6.026735295929472 4 4.78834618680377 5.7372055934674435 5 3.472150814053128 5.200240932518361 6 2.3581833761782347 4.238222027529278 7 1.6852327906312483 3.533562858061999 8 1.2305055698371894 2.948685480393432 9 0.9003141959440161 2.427121335532755 >10 4.8397600685518425 26.699019158242017 >50 0.3336189660097115 6.847069452905222 >100 0.14738646101113967 7.5534397908267685 >500 0.0034275921165381317 0.64545274094963 >1k 0.004570122822050843 3.12938486916406 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4237 1.45004414814612 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2438 0.8343657383007413 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1288 0.4407969938192595 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1181 0.40417798889793904 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 685 0.23443007823462175 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 608 0.20807808403890513 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 593 0.20294457867610316 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 475 0.16256100315539462 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 397 0.13586677526882457 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 386 0.13210220466943648 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 383 0.1310755035968761 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 364 0.1245730634706603 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 336 0.11499052012676335 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 334 0.11430605274505644 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 317 0.10848808000054758 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 316 0.10814584630969412 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 302 0.10335457463774564 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.422336908534624E-4 0.0 24-25 0.0 0.0 0.0 6.844673817069248E-4 0.0 26-27 0.0 0.0 0.0 0.002737869526827699 0.0 28-29 0.0 0.0 0.0 0.004106804290241548 0.0 30-31 0.0 0.0 0.0 0.011807062334444451 0.0 32-33 0.0 0.0 0.0 0.028918746877117575 0.0 34-35 0.0 0.0 0.0 0.046372665110644154 0.0 36-37 0.0 0.0 0.0 0.06433993388045092 0.0 38-39 0.0 0.0 0.0 0.09616766712982293 0.0 40-41 0.0 0.0 0.0 0.12166407709840588 0.0 42-43 0.0 0.0 0.0 0.1584541988651531 0.0 44-45 0.0 0.0 0.0 0.19541543747732704 0.0 46-47 0.0 0.0 0.0 0.2464082574144929 0.0 48-49 0.0 0.0 0.0 0.30133676479647364 0.0 50-51 0.0 0.0 0.0 0.35558080479674736 0.0 52-53 0.0 0.0 0.0 0.4211185565951855 0.0 54-55 0.0 0.0 0.0 0.49384321590154623 0.0 56-57 0.0 0.0 0.0 0.576663769088084 0.0 58-59 0.0 0.0 0.0 0.6836117974797911 0.0 60-61 0.0 0.0 0.0 0.7926132280166189 0.0 62-63 0.0 0.0 0.0 0.9034969438531406 0.0 64-65 0.0 0.0 0.0 1.0335457463774564 0.0 66-67 0.0 0.0 0.0 1.194224464233157 0.0 68-69 0.0 0.0 0.0 1.3528497799437367 0.0 70-71 0.0 0.0 0.0 1.533549168714365 0.0 72-73 0.0 0.0 0.0 1.7204087639203554 0.0 74-75 0.0 0.0 0.0 1.9486786357196149 0.0 76-77 0.0 0.0 0.0 2.1991936974243496 0.0 78-79 0.0 0.0 0.0 2.457066783482433 0.0 80-81 0.0 0.0 0.0 2.758916898815187 0.0 82-83 0.0 0.0 0.0 3.0705206743372644 0.0 84-85 0.0 0.0 0.0 3.378188762414527 0.0 86-87 0.0 0.0 0.0 3.750710134908521 0.0 88-89 0.0 0.0 0.0 4.106290939705269 0.0 90-91 0.0 0.0 0.0 4.501570852641017 0.0 92-93 0.0 0.0 0.0 4.921149357627362 0.0 94-95 0.0 0.0 0.0 5.386587177188071 0.0 96-97 0.0 0.0 0.0 5.875296887726815 0.0 98-99 0.0 0.0 0.0 6.400967836877734 0.0 100-101 0.0 0.0 0.0 6.955215299214916 0.0 102-103 0.0 1.711168454267312E-4 0.0 7.527430030321906 0.0 104-105 0.0 3.422336908534624E-4 0.0 8.134552597895947 0.0 106-107 0.0 3.422336908534624E-4 0.0 8.738595062252308 0.0 108-109 0.0 3.422336908534624E-4 0.0 9.430933818848864 0.0 110-111 0.0 3.422336908534624E-4 0.0 10.085113518915257 0.0 112-113 0.0 3.422336908534624E-4 0.0 10.813900163587704 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 595 0.0 62.990486 1 CATGGGT 285 0.0 60.534718 4 GGATCGC 30 0.0042359265 59.491016 8 CACGTCG 15 0.0042380444 59.491016 36-37 GGTATCA 710 0.0 58.65311 1 GGGTATG 110 3.6379788E-12 54.08274 7 CAGTGCG 55 1.3094492E-5 54.08274 9 ATGGGTA 195 0.0 51.86396 5 GTACATG 2570 0.0 51.85209 1 GAGAACA 290 0.0 51.28536 1 GGTATGG 85 6.187656E-8 48.9926 8 GGTACCT 100 4.134563E-9 47.59281 8 ACATGGG 2750 0.0 46.294827 3 TACATGG 2890 0.0 45.69898 2 GTATCAA 905 0.0 45.357788 2 TGGGAAC 260 0.0 43.474205 6 CATGGGG 740 0.0 43.412365 4 ATATAGG 165 0.0 43.266193 2 TGGGTAC 110 9.602445E-9 43.26619 6 GCTTACT 170 0.0 41.993656 8 >>END_MODULE