FastQCFastQC Report
Thu 26 May 2016
SRR936200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292198
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63912.187215518244478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59572.038686096414075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51931.7772195566020301No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.6149939424636719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.5458627369112724No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.5164306394978747No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.3511317668156524No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.27720928959130453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6480.22176743167304364No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6460.22108296429133673No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG4040.13826241110479878No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT3950.13518230788711763No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3680.12594199823407418No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG3480.11909732441700491No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3440.11772838965359105No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3430.11738615596273759No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3260.11156818321822874No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC3220.11019924845481488No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3100.10609244416457334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.091.639441
TCAGCCG250.002052857571.421475
GAGGTAC250.002055647871.3970266
ATATAGG1103.6379788E-1254.1071742
GTATCAA27150.053.4893762
AACCGCA350.007791753450.997879
CAGCCGA350.007791753450.997876
GCCTATC856.182199E-848.997967
TGTTACG255.3315336E-447.59801536-37
GTACATG21200.044.0844761
GCTTACT1404.0017767E-1142.4982268
ACATGGG21500.042.354593
AGGCTTA1551.8189894E-1242.2240456
CCACGCA853.4647765E-642.012634
ATGGGAT6500.041.2046935
GTGGTAT3650.040.772661
CATGGGT2100.039.6785934
GGTACTC600.001304980839.6785933
GACTTGA1202.0676453E-839.6650127
CCAACGT300.001308252839.66501214-15
Produced by FastQC (version 0.11.5)