##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936200_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 292198 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.527936536184367 31.0 31.0 34.0 30.0 34.0 2 31.357110589394864 31.0 31.0 34.0 28.0 34.0 3 31.628347901080772 31.0 31.0 34.0 30.0 34.0 4 35.54680730189803 37.0 35.0 37.0 33.0 37.0 5 35.17812921375232 37.0 35.0 37.0 33.0 37.0 6 35.407809088357894 37.0 35.0 37.0 33.0 37.0 7 35.24919061732113 37.0 35.0 37.0 32.0 37.0 8 35.33014257455561 37.0 35.0 37.0 32.0 37.0 9 36.989565294765875 39.0 37.0 39.0 33.0 39.0 10-11 36.87562885440694 39.0 37.0 39.0 32.0 39.0 12-13 36.80256709491509 39.0 37.0 39.0 32.0 39.0 14-15 37.81267154463754 40.0 37.0 41.0 32.0 41.0 16-17 37.752310077413256 40.0 37.0 41.0 32.0 41.0 18-19 37.99356258427504 40.0 37.0 41.0 33.0 41.0 20-21 38.067880341412334 40.0 37.0 41.0 33.0 41.0 22-23 37.90351747787459 40.0 37.0 41.0 32.5 41.0 24-25 37.824704481207945 40.0 37.0 41.0 32.0 41.0 26-27 37.580120329365705 40.0 36.0 41.0 32.0 41.0 28-29 37.35539257626678 39.5 36.0 41.0 32.0 41.0 30-31 36.97373869773236 39.0 35.5 41.0 31.5 41.0 32-33 36.49770190076592 38.0 35.0 41.0 30.5 41.0 34-35 36.17143170042232 38.0 35.0 40.5 30.0 41.0 36-37 35.87561516505931 38.0 35.0 40.0 30.0 41.0 38-39 35.76745562940198 38.0 35.0 40.5 30.0 41.0 40-41 35.68316176017632 38.0 35.0 41.0 29.5 41.0 42-43 35.457918945372654 38.0 35.0 41.0 27.5 41.0 44-45 35.12193101937727 38.0 35.0 41.0 25.5 41.0 46-47 34.54896850765577 38.0 34.0 41.0 22.5 41.0 48-49 34.10875673344786 38.0 33.5 41.0 20.0 41.0 50-51 33.946724481344845 38.0 33.0 40.0 20.0 41.0 52-53 33.85886282589203 38.0 33.0 40.0 20.0 41.0 54-55 33.69087912990506 37.5 33.0 40.0 20.0 41.0 56-57 33.48546704631791 37.0 33.0 40.0 18.5 41.0 58-59 33.26621845460954 37.0 33.0 40.0 18.0 41.0 60-61 33.072442316511406 36.0 33.0 40.0 17.0 41.0 62-63 32.83926310241685 36.0 33.0 40.0 15.0 41.0 64-65 32.60715507977467 35.5 33.0 39.5 13.0 41.0 66-67 32.30737205593468 35.0 32.0 39.0 11.0 41.0 68-69 32.00800313486061 35.0 32.0 39.0 8.0 41.0 70-71 31.68814981622051 35.0 32.0 38.5 6.5 40.0 72-73 31.387644679292805 35.0 31.5 37.0 2.0 40.0 74-75 31.186031389674124 35.0 32.0 37.0 2.0 39.0 76-77 30.874891340803153 35.0 31.5 36.5 2.0 39.0 78-79 30.52244026310926 35.0 31.0 36.0 2.0 39.0 80-81 30.22832120685289 35.0 31.0 36.0 2.0 37.5 82-83 29.932544028364326 35.0 31.0 35.0 2.0 37.0 84-85 29.626234608039752 35.0 31.0 35.0 2.0 37.0 86-87 29.39064777993005 35.0 30.5 35.0 2.0 36.0 88-89 29.174623029589526 34.5 30.0 35.0 2.0 36.0 90-91 28.965549045510237 34.0 30.0 35.0 2.0 36.0 92-93 28.788677540571804 34.0 30.0 35.0 2.0 35.5 94-95 28.607247482871202 34.0 29.5 35.0 2.0 35.0 96-97 28.47105216325916 34.0 29.0 35.0 2.0 35.0 98-99 28.325529264402903 34.0 29.0 35.0 2.0 35.0 100-101 28.176640497197106 34.0 29.0 35.0 2.0 35.0 102-103 27.127357134545754 33.0 26.0 34.5 2.0 35.0 104-105 27.77718019972758 34.0 29.0 35.0 2.0 35.0 106-107 27.843701873387225 34.0 29.0 35.0 2.0 35.0 108-109 27.738962963469977 34.0 29.0 35.0 2.0 35.0 110-111 27.623373534384218 34.0 29.0 35.0 2.0 35.0 112-113 27.494685110781045 34.0 29.0 35.0 2.0 35.0 114-115 27.405688950643057 34.0 29.0 35.0 2.0 35.0 116-117 27.24374054579429 34.0 27.0 35.0 2.0 35.0 118-119 27.06597581092273 34.0 27.0 35.0 2.0 35.0 120-121 26.848373363267374 34.0 27.0 35.0 2.0 35.0 122-123 26.59818000123204 34.0 25.0 35.0 2.0 35.0 124-125 24.68603652317949 31.5 13.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14472495834934662 1101 2 0.6312944624834493 1101 3 0.4291303563394919 1101 4 0.23433948599638654 1101 5 0.6446580791555689 1101 6 0.25813874153838867 1101 7 0.4926209196292817 1101 8 0.3637625487899925 1101 9 0.36615884716367475 1101 10-11 0.4967189851729259 1101 12-13 0.7575914090280023 1101 14-15 0.6683328009435243 1101 16-17 0.620972864573595 1101 18-19 0.5443289274424572 1101 20-21 0.2936700103452239 1101 22-23 0.4834153441514104 1101 24-25 0.3934744147144471 1101 26-27 0.49573250553505943 1101 28-29 0.28249922480418377 1101 30-31 0.219203025313206 1101 32-33 0.13480985685070124 1101 34-35 0.2496522316980858 1101 36-37 0.08464548937912753 1101 38-39 -0.01672810488743437 1101 40-41 0.06145486366559538 1101 42-43 0.16173324527406407 1101 44-45 0.22812409089158336 1101 46-47 -0.07260390188390886 1101 48-49 -0.38013381573091465 1101 50-51 0.023037043100657684 1101 52-53 0.03993764082711948 1101 54-55 -0.06811634439104353 1101 56-57 -0.06559314855664411 1101 58-59 0.1531566233924977 1101 60-61 0.1910472888809096 1101 62-63 -0.008769516617661566 1101 64-65 0.05640028503454886 1101 66-67 0.10224246376739643 1101 68-69 0.025230411916112416 1101 70-71 0.03743168042881706 1101 72-73 -0.019048842350535722 1101 74-75 0.06192673399737458 1101 76-77 -0.24409486430421268 1101 78-79 -0.2575609888342498 1101 80-81 -0.4504009389766246 1101 82-83 -0.38824548344178567 1101 84-85 -0.5120457277567745 1101 86-87 -0.4082872492453866 1101 88-89 -0.4874291981747554 1101 90-91 -0.40699501438766816 1101 92-93 -0.1772574860916798 1101 94-95 -0.12807710356867474 1101 96-97 -0.2864338726517488 1101 98-99 -0.4707028869580796 1101 100-101 -0.6153439014750468 1101 102-103 -0.5455120473908188 1101 104-105 -0.6414745924194492 1101 106-107 -0.540578576823151 1101 108-109 -0.4180509421799101 1101 110-111 -0.4146877479600981 1101 112-113 -0.6075049476827381 1101 114-115 -0.9025600957897524 1101 116-117 -0.9343282875958216 1101 118-119 -0.7729117250853541 1101 120-121 -0.8002545680021207 1101 122-123 -0.6492548893484873 1101 124-125 -0.7229321275930118 1102 1 0.3716953515481798 1102 2 0.6829115498659952 1102 3 0.45952647110669176 1102 4 0.1344536096823319 1102 5 0.47314073688003333 1102 6 0.38274974064852074 1102 7 0.275630064606581 1102 8 0.2275229996539636 1102 9 0.24121603469352237 1102 10-11 0.44370677343578535 1102 12-13 0.4648365034691224 1102 14-15 0.35789877901368783 1102 16-17 0.47133509415828456 1102 18-19 0.42992873036543244 1102 20-21 0.3576295492252015 1102 22-23 0.6707566644669356 1102 24-25 0.7290634085658496 1102 26-27 0.5801664369703587 1102 28-29 0.5576554535945206 1102 30-31 0.024664874318020225 1102 32-33 0.37955237471080494 1102 34-35 0.5876569273598662 1102 36-37 0.23815441566125628 1102 38-39 0.16780285681828389 1102 40-41 0.21334494294887918 1102 42-43 0.24936967838099378 1102 44-45 0.38798523202789426 1102 46-47 0.6746850321405304 1102 48-49 0.5146813491451354 1102 50-51 0.8571253356192727 1102 52-53 0.5742627274253778 1102 54-55 0.6102006580271677 1102 56-57 0.16693511842239417 1102 58-59 0.6506881330930057 1102 60-61 0.3483519589025761 1102 62-63 0.37633759374044473 1102 64-65 0.6552002267158414 1102 66-67 0.532868334149029 1102 68-69 0.25518209787990287 1102 70-71 0.42507012452580994 1102 72-73 0.45141222739562537 1102 74-75 0.620004126949862 1102 76-77 0.5651602147025621 1102 78-79 0.5020452593118669 1102 80-81 0.4082088635944281 1102 82-83 0.33189508476345964 1102 84-85 0.49757937342116065 1102 86-87 0.49848576527898913 1102 88-89 0.40678769715824004 1102 90-91 0.3586215803023123 1102 92-93 0.5964533435925397 1102 94-95 0.3947350649321848 1102 96-97 0.33903179723417765 1102 98-99 0.6794225485469596 1102 100-101 0.7080584926086217 1102 102-103 0.7520818030015732 1102 104-105 0.6519338849091483 1102 106-107 0.5879991001816798 1102 108-109 0.5881680445827477 1102 110-111 0.6674989020823858 1102 112-113 0.7207489599223251 1102 114-115 0.8170852661130468 1102 116-117 0.8825358750530548 1102 118-119 0.6713235205319457 1102 120-121 0.7671135990307114 1102 122-123 0.7527495649944989 1102 124-125 0.4725159964046348 1103 1 0.012154681873877138 1103 2 0.42559709990121064 1103 3 0.2999267909056762 1103 4 0.1142472631953595 1103 5 0.2902128629722043 1103 6 0.22635756810935703 1103 7 0.4083180892767899 1103 8 0.2924345790421583 1103 9 0.23208367979279387 1103 10-11 0.1471598410313888 1103 12-13 0.2236976003034954 1103 14-15 0.3531184504582967 1103 16-17 0.5096181733656309 1103 18-19 0.25795349575099635 1103 20-21 0.20177037499185957 1103 22-23 0.1470215836605817 1103 24-25 0.257785830662435 1103 26-27 0.03429051136168937 1103 28-29 0.07528008953764243 1103 30-31 -0.07698313740793594 1103 32-33 -0.1325566805496905 1103 34-35 -0.1753468036282655 1103 36-37 -0.24507755281570098 1103 38-39 -0.4854389150203673 1103 40-41 -0.40436916056700767 1103 42-43 -0.3506531644035391 1103 44-45 -0.22453213800618244 1103 46-47 -0.09492645016582912 1103 48-49 -0.4523203450280562 1103 50-51 -0.31469761376492755 1103 52-53 -0.31127350694158906 1103 54-55 -0.1920093813766357 1103 56-57 -0.3260526226165723 1103 58-59 -0.23456018701054404 1103 60-61 -0.14308913526679845 1103 62-63 -0.31919426242615145 1103 64-65 0.04501472426975539 1103 66-67 -0.18754550096413425 1103 68-69 -0.20154748162250513 1103 70-71 -0.13057347092847493 1103 72-73 -0.03616938796995228 1103 74-75 0.07685505874511378 1103 76-77 0.08118585572820436 1103 78-79 0.18566555512592942 1103 80-81 0.39304458590231306 1103 82-83 0.27577797742140575 1103 84-85 0.24135233517853294 1103 86-87 0.021209576978300504 1103 88-89 0.0889898367657267 1103 90-91 -0.13685745192505294 1103 92-93 -0.09306800489317268 1103 94-95 0.18800786488649734 1103 96-97 0.1912556374869716 1103 98-99 0.20064968196785316 1103 100-101 0.21841388826468489 1103 102-103 0.2928038253494307 1103 104-105 0.26504611912383425 1103 106-107 0.14648852587600558 1103 108-109 -0.016765996876909384 1103 110-111 -0.08062608602032739 1103 112-113 -0.08163240036549624 1103 114-115 -0.1623467099781699 1103 116-117 -0.3110019963801385 1103 118-119 -0.3060169131002475 1103 120-121 -0.44369912820740254 1103 122-123 -0.5734001884937889 1103 124-125 0.07297427976937598 1104 1 0.04832255767458449 1104 2 0.3453050617258029 1104 3 0.1738953349606156 1104 4 7.27114038063803E-4 1104 5 0.5049079950539408 1104 6 0.20954506695560582 1104 7 0.24691254018225806 1104 8 0.0810299529507077 1104 9 0.21929547208017652 1104 10-11 0.22852256278287797 1104 12-13 -0.053401770208402866 1104 14-15 0.07844888556194718 1104 16-17 -6.063932844213582E-4 1104 18-19 0.05141540341377038 1104 20-21 -0.01001729737895829 1104 22-23 -0.1384978824744394 1104 24-25 -0.13007160228139014 1104 26-27 -0.4901506102716553 1104 28-29 -0.026750978746129306 1104 30-31 -0.3531341222522286 1104 32-33 -0.021134055898130555 1104 34-35 -0.0864592992440052 1104 36-37 0.08734892998874955 1104 38-39 -0.10956898461479625 1104 40-41 0.09708226178074142 1104 42-43 0.3564687273835716 1104 44-45 0.651289334737541 1104 46-47 0.7048095709524773 1104 48-49 0.6157049821765384 1104 50-51 0.5572749361233917 1104 52-53 0.28228439304179886 1104 54-55 0.34811356263399773 1104 56-57 0.15294674974132505 1104 58-59 0.14697698232360068 1104 60-61 0.4223633208444042 1104 62-63 0.3224982483268235 1104 64-65 0.3394180950770149 1104 66-67 0.20376165600175966 1104 68-69 0.4075076401216009 1104 70-71 0.5525480331973469 1104 72-73 0.45026015920473483 1104 74-75 0.6819897248948976 1104 76-77 0.6753114026075302 1104 78-79 0.5827068687439159 1104 80-81 0.4888776884848731 1104 82-83 0.5163125808069822 1104 84-85 0.5831426842582985 1104 86-87 0.7046795147678964 1104 88-89 0.39920425038186735 1104 90-91 0.36090888061450244 1104 92-93 0.3817501634995111 1104 94-95 0.47374192923827607 1104 96-97 0.25448827118396977 1104 98-99 0.5160236828170177 1104 100-101 0.5484982507464551 1104 102-103 0.22784748339525152 1104 104-105 0.2868220693555088 1104 106-107 0.2778618621629505 1104 108-109 0.2129233202854124 1104 110-111 0.27359019206203783 1104 112-113 0.29916175148697377 1104 114-115 0.23185867666781945 1104 116-117 0.18894031592718719 1104 118-119 0.28584727622614636 1104 120-121 0.33688227143447236 1104 122-123 0.614244030737904 1104 124-125 0.7385956454447395 1105 1 0.30635702221355743 1105 2 0.36450249588815353 1105 3 0.260997551332661 1105 4 0.08715728116975896 1105 5 0.12704019872657568 1105 6 0.23984753546198334 1105 7 0.28666077663235257 1105 8 0.06764458601182355 1105 9 0.11927600582632181 1105 10-11 0.19880871671460199 1105 12-13 0.08545982472666225 1105 14-15 0.14460170492439062 1105 16-17 0.3916061782077165 1105 18-19 0.2870289148478804 1105 20-21 0.2287594803071542 1105 22-23 0.17625290312795272 1105 24-25 0.08325536589592275 1105 26-27 -0.1024616253904469 1105 28-29 -0.1298618715056108 1105 30-31 -0.15561653310142276 1105 32-33 -0.33999129410018725 1105 34-35 -0.47027820746524895 1105 36-37 -0.3354951823964143 1105 38-39 -0.4862734405330045 1105 40-41 -0.7098009128871752 1105 42-43 -0.6578227473241967 1105 44-45 -0.9691627458342111 1105 46-47 -0.6569197739617323 1105 48-49 -0.5830107085510292 1105 50-51 -0.3522741883586704 1105 52-53 -0.45220285656193226 1105 54-55 -0.3191965718685452 1105 56-57 -0.24019278622025553 1105 58-59 -0.20932266821079537 1105 60-61 -0.3402526655848419 1105 62-63 -0.25751732467625743 1105 64-65 -0.565449858834306 1105 66-67 -0.6682963905133121 1105 68-69 -0.2964036864572499 1105 70-71 -0.31766767143469465 1105 72-73 -0.5342664863889404 1105 74-75 -0.5225635245790521 1105 76-77 -0.6612346350828453 1105 78-79 -0.6372270386894137 1105 80-81 -0.5396844042301119 1105 82-83 -0.6887134205463852 1105 84-85 -0.5077902127837568 1105 86-87 -0.7138148168443763 1105 88-89 -0.5791395422005614 1105 90-91 -0.4570584716968895 1105 92-93 -0.4159514280984844 1105 94-95 -0.3093785112552432 1105 96-97 -0.30013774500082846 1105 98-99 -0.17766090825260505 1105 100-101 -0.09503756252582107 1105 102-103 -0.05382689252591888 1105 104-105 -0.12501202254374988 1105 106-107 -0.2845622426211136 1105 108-109 -0.224837967824417 1105 110-111 -0.08985171970948258 1105 112-113 -0.014200904822413207 1105 114-115 -0.09022903271027616 1105 116-117 -0.2552309314888994 1105 118-119 -0.01607432111903151 1105 120-121 -0.28175991101067765 1105 122-123 -0.11492012539999052 1105 124-125 0.10982637617082958 1106 1 0.27924869896789417 1106 2 0.7418364161405258 1106 3 0.3063370473159317 1106 4 0.1655293144774106 1106 5 0.3286744014337373 1106 6 0.35883620058799437 1106 7 0.4360958670545685 1106 8 0.4206397072472825 1106 9 0.300459748168862 1106 10-11 0.3651085589801042 1106 12-13 0.3967745233804223 1106 14-15 0.20140904874889287 1106 16-17 0.30128484003230227 1106 18-19 0.1917141795116848 1106 20-21 0.16224046046195184 1106 22-23 0.04232072895972294 1106 24-25 0.29197386485046906 1106 26-27 0.40608538315656517 1106 28-29 0.257972397229949 1106 30-31 0.14523908481427839 1106 32-33 -0.17849685148986794 1106 34-35 -0.0033382566197204255 1106 36-37 0.17265748991934515 1106 38-39 0.15024484566339424 1106 40-41 -0.07637770757555273 1106 42-43 -0.11026854901892591 1106 44-45 -0.0471817106557495 1106 46-47 -0.03830251854189726 1106 48-49 0.1908420481343427 1106 50-51 -0.12880017786748965 1106 52-53 -0.06341025907834563 1106 54-55 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-0.5100557772945251 2216 44-45 -0.528983177922342 2216 46-47 -0.6621099126981278 2216 48-49 -0.8582309718689061 2216 50-51 -0.7454022450509541 2216 52-53 -0.5994707747900208 2216 54-55 -1.0790641870360886 2216 56-57 -1.2410139532755764 2216 58-59 -1.3638449848999912 2216 60-61 -0.8371789601705757 2216 62-63 -0.6492230837340429 2216 64-65 -0.5666317042371389 2216 66-67 -0.7492267138081417 2216 68-69 -0.6137938270083865 2216 70-71 -0.728428135643604 2216 72-73 -0.7025898538090232 2216 74-75 -0.7004754918412495 2216 76-77 -0.5172922165870766 2216 78-79 -0.5158780563014034 2216 80-81 -0.5781408823761787 2216 82-83 -0.6034345413259956 2216 84-85 -0.7845439261828417 2216 86-87 -0.565358247961619 2216 88-89 -0.8034610585921449 2216 90-91 -0.6642693165462177 2216 92-93 -0.7016109477384411 2216 94-95 -0.7513047604726388 2216 96-97 -0.8029868771043809 2216 98-99 -0.8924119721403088 2216 100-101 -1.1480323270187398 2216 102-103 -0.894141658107678 2216 104-105 -0.9444301281431109 2216 106-107 -0.9736507018213629 2216 108-109 -1.1443477174818817 2216 110-111 -0.9907785950100454 2216 112-113 -1.1161095511217134 2216 114-115 -1.147583695215154 2216 116-117 -0.9821928855942836 2216 118-119 -0.8726831280222775 2216 120-121 -1.0914986800767217 2216 122-123 -1.097457849179353 2216 124-125 -0.9052132303948781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 14.0 4 58.0 5 168.0 6 357.0 7 709.0 8 943.0 9 1380.0 10 2066.0 11 2686.0 12 3009.0 13 3767.0 14 5151.0 15 3859.0 16 1891.0 17 1768.0 18 1881.0 19 1861.0 20 1946.0 21 2193.0 22 2277.0 23 2680.0 24 2951.0 25 3519.0 26 4070.0 27 4828.0 28 5572.0 29 6476.0 30 7972.0 31 9408.0 32 11518.0 33 14537.0 34 19220.0 35 28554.0 36 42960.0 37 64306.0 38 25574.0 39 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.706891474513945 17.408454948260864 12.752275357654035 20.132378219571155 2 20.053389003918614 22.974041308030596 35.313403720118416 21.659165967932374 3 17.03057515794085 28.780484466012773 33.65218105531181 20.53675932073457 4 11.16845426731189 17.6435156982594 36.053634864030556 35.13439517039815 5 9.804975771792543 41.53416776535452 35.151618923685426 13.50923753916751 6 27.7308537361652 39.532782565246855 16.748916830368447 15.987446868219497 7 23.7715521666815 36.01941149494521 23.392015003525007 16.817021334848288 8 28.44235757944955 30.773653481543338 20.714378606287518 20.069610332719595 9 26.568628122026844 10.885427004976078 20.471050452090704 42.074894420906375 10-11 23.83674768478908 25.83231233615562 29.01029438942087 21.320645589634427 12-13 26.06588683016311 26.366881361268728 29.409167756110584 18.158064052457583 14-15 23.045503391535878 26.631428004298456 29.616903606458635 20.706164997707035 16-17 19.475321528552556 27.54365190726836 31.077385882175783 21.9036406820033 18-19 18.075756849807323 23.408442220685973 37.45011943955811 21.065681489948595 20-21 19.47463706117085 22.95994496882251 39.09694795994497 18.468470010061672 22-23 19.106051376121673 23.07784447532153 38.93866487792524 18.87743927063156 24-25 19.045304895995184 23.614124668888902 38.88886987590606 18.45170055920985 26-27 18.56908671517259 23.416484712421028 39.07316271843065 18.94126585397573 28-29 18.577471440598497 22.381398914434733 39.76019685281898 19.28093279214779 30-31 18.79153861422734 23.052519182198374 39.125182239440385 19.03075996413391 32-33 18.78400947302856 23.37969459065428 38.73092902757719 19.105366908739967 34-35 19.043080377004635 23.16528518333459 38.619189727513536 19.172444712147243 36-37 19.412179412590095 23.066892997214218 37.66863565116804 19.852291939027648 38-39 18.80557019555233 23.591537245292578 38.210733817479934 19.392158741675168 40-41 18.68852627328045 23.441296655007907 38.56340563590442 19.306771435807228 42-43 19.420388062102255 23.641192283933187 37.016483826752975 19.921935827211577 44-45 19.16633296711947 23.676924077363534 37.00129030491583 20.155452650601173 46-47 19.710890960336926 23.6665885167546 36.37068385736557 20.251836665542903 48-49 20.03107662715919 24.34432765990944 34.97694921983291 20.647646493098456 50-51 19.83537686206395 24.25213693496359 34.901816501672755 21.0106697012997 52-53 19.959272378801103 24.34741687629413 34.79422283827027 20.899087906634495 54-55 20.207677709384686 24.803419768741218 34.652367433479306 20.336535088394793 56-57 20.188404805092578 24.681542831719085 34.26948218624867 20.860570176939664 58-59 19.8317904139359 23.66587381714266 35.27729769503243 21.225038073889014 60-61 20.45308705986232 24.627347101512516 34.8449924793503 20.07457335927487 62-63 19.831620735974813 24.616740389633723 35.214024760649906 20.337614113741562 64-65 20.473792375310364 24.039726826730735 35.13965820828863 20.34682258967027 66-67 20.248875746935255 24.23389939560703 34.52842974188381 20.9887951155739 68-69 20.442508919480833 24.59928644153355 34.507139862592936 20.45106477639268 70-71 20.778831871975466 23.905008316392536 34.802904919334416 20.51325489229758 72-73 19.94041660463628 24.616908884633748 34.289371328442776 21.1533031822872 74-75 20.016803731728935 24.864902788187422 34.07820751068628 21.04008596939736 76-77 19.722139515463883 24.726297669038356 34.5937843304346 20.95777848506316 78-79 19.4493450491534 24.7438804233438 34.31429084779986 21.49248367970294 80-81 19.57816275265402 25.5217352617061 33.603754988056046 21.29634699758383 82-83 20.653838585203502 24.549320237168352 33.196040349421196 21.60080082820695 84-85 19.480146134036055 24.450414531267377 33.59850785855457 22.470931476141992 86-87 19.720736830397247 24.80890493587385 33.577631567689664 21.892726666039238 88-89 20.09024748756402 24.567884967821314 33.39003033917244 21.951837205442228 90-91 19.954653570389638 24.862163965844726 32.93571074111467 22.247471722650968 92-93 20.23603978829315 24.858271745212555 32.55275131632309 22.352937150171204 94-95 19.843563641466073 24.43295670193415 32.80406440208696 22.919415254512817 96-97 20.41116307403776 24.62084460575197 32.73424813181586 22.233744188394414 98-99 20.00263521294745 24.787771242982036 32.52965042360192 22.679943120468586 100-101 19.865227680687482 24.645572414453973 32.43998473629903 23.049215168559513 102-103 20.516365727114678 23.9984462484086 32.39982751303919 23.08536051143753 104-105 19.57905183993703 25.0020961849434 32.236757672464684 23.182094302654882 106-107 19.991820586795896 24.266676249242806 32.345985756185044 23.39551740777626 108-109 20.370709436971353 24.55265549039773 31.340211125047702 23.73642394758322 110-111 20.235935906474378 24.705850142711448 31.238064600031485 23.82014935078269 112-113 19.99082722670783 24.19815243917048 32.15039925522557 23.660621078896117 114-115 19.769121497886324 24.744732923719386 31.231066764791453 24.255078813602836 116-117 19.735204971410283 25.06790164658284 31.22021013142199 23.976683250584887 118-119 19.318580481535548 24.14899570283688 31.389142261598145 25.14328155402942 120-121 19.477390374331552 24.585754010695187 31.096983957219248 24.839871657754014 122-123 19.259577424538264 24.775441544640458 31.053837558118243 24.911143472703035 124-125 19.427522194766347 24.706693681422106 30.465183136769042 25.4006009870425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 452.0 1 324.0 2 600.0 3 1060.0 4 782.5 5 661.0 6 1185.0 7 2949.0 8 4413.0 9 4681.0 10 3577.5 11 1827.5 12 1305.0 13 1139.5 14 1117.0 15 1078.5 16 1018.5 17 1029.5 18 1044.0 19 996.5 20 961.0 21 963.5 22 1005.0 23 1117.5 24 1182.0 25 1235.0 26 1379.0 27 1593.5 28 1806.5 29 2214.0 30 3024.5 31 3551.5 32 4490.0 33 6183.5 34 7453.5 35 8228.0 36 9884.0 37 11510.5 38 10994.5 39 10318.0 40 9802.5 41 8981.0 42 7870.0 43 7585.5 44 8193.0 45 8434.5 46 7764.0 47 6711.5 48 6755.0 49 6679.5 50 6838.0 51 6916.0 52 6779.5 53 6698.5 54 6596.5 55 6772.5 56 6420.5 57 6004.0 58 5849.0 59 5457.5 60 4898.5 61 4400.5 62 3955.0 63 3229.0 64 2440.5 65 1944.5 66 1603.5 67 1408.5 68 1066.0 69 661.5 70 557.0 71 560.0 72 542.0 73 484.5 74 401.5 75 332.5 76 217.5 77 134.5 78 74.0 79 25.5 80 18.5 81 11.5 82 5.0 83 4.0 84 3.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05338845577314013 2 0.0010267010725603873 3 0.0 4 0.0 5 0.0626287654261836 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.555842271336559E-4 44-45 0.006502440126215785 46-47 0.008384725425909829 48-49 0.00581797274450886 50-51 0.005304622208228667 52-53 0.004449037981095011 54-55 0.004962388517375205 56-57 0.002737869526827699 58-59 0.0010267010725603873 60-61 8.555842271336559E-4 62-63 1.711168454267312E-4 64-65 8.555842271336559E-4 66-67 0.0013689347634138495 68-69 1.711168454267312E-4 70-71 0.0013689347634138495 72-73 8.555842271336559E-4 74-75 3.422336908534624E-4 76-77 3.422336908534624E-4 78-79 1.711168454267312E-4 80-81 0.0 82-83 1.711168454267312E-4 84-85 1.711168454267312E-4 86-87 1.711168454267312E-4 88-89 5.133505362801936E-4 90-91 0.0010267010725603873 92-93 5.133505362801936E-4 94-95 8.555842271336559E-4 96-97 8.555842271336559E-4 98-99 5.133505362801936E-4 100-101 5.133505362801936E-4 102-103 6.844673817069248E-4 104-105 1.711168454267312E-4 106-107 3.422336908534624E-4 108-109 5.133505362801936E-4 110-111 0.0 112-113 0.00992477703475041 114-115 0.018138385615233506 116-117 0.01625610031553946 118-119 0.009753660189323677 120-121 0.0035934537539613546 122-123 0.004620154826521742 124-125 0.004106804290241549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 292198.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.457723872168874 #Duplication Level Percentage of deduplicated Percentage of total 1 52.63411289263674 12.346764864920361 2 12.374713683381234 5.805652331638136 3 8.616488919364485 6.063696534541647 4 6.321579154691216 5.93159432987221 5 4.566476518389916 5.355957261856687 6 3.233007017492669 4.550339153587635 7 2.2759435682710123 3.737191904119809 8 1.625257137855069 3.049986652886057 9 1.200706126081438 2.534924948151596 >10 6.502487489605066 27.74933435547129 >50 0.4566476518389916 7.340912668806768 >100 0.17799045854427148 6.782045051643064 >500 0.004376814554367331 0.7200596855556849 >1k 0.005835752739156441 2.028419085688472 >5k 0.004376814554367331 6.003121171260584 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6391 2.187215518244478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5957 2.038686096414075 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5193 1.7772195566020301 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1797 0.6149939424636719 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1595 0.5458627369112724 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1509 0.5164306394978747 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1026 0.3511317668156524 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 810 0.27720928959130453 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 648 0.22176743167304364 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 646 0.22108296429133673 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 404 0.13826241110479878 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 395 0.13518230788711763 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 368 0.12594199823407418 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 348 0.11909732441700491 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 344 0.11772838965359105 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 343 0.11738615596273759 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 326 0.11156818321822874 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 322 0.11019924845481488 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 310 0.10609244416457334 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.422336908534624E-4 0.0 24-25 0.0 0.0 0.0 6.844673817069248E-4 0.0 26-27 0.0 0.0 0.0 0.002737869526827699 0.0 28-29 0.0 0.0 0.0 0.004106804290241548 0.0 30-31 0.0 0.0 0.0 0.011807062334444451 0.0 32-33 0.0 0.0 0.0 0.028234279495410644 0.0 34-35 0.0 0.0 0.0 0.0455170808835105 0.0 36-37 0.0 0.0 0.0 0.06348434965331727 0.0 38-39 0.0 0.0 0.0 0.09633878397524966 0.0 40-41 0.0 0.0 0.0 0.12251966132553954 0.0 42-43 0.0 0.0 0.0 0.15948089993771347 0.0 44-45 0.0 0.0 0.0 0.1974688396224478 0.0 46-47 0.0 0.0 0.0 0.24931724378674733 0.0 48-49 0.0 0.0 0.0 0.3044168680141548 0.0 50-51 0.0 0.0 0.0 0.359003141705282 0.0 52-53 0.0 0.0 0.0 0.4240275429674399 0.0 54-55 0.0 0.0 0.0 0.4981211370372145 0.0 56-57 0.0 0.0 0.0 0.5805994565328989 0.0 58-59 0.0 0.0 0.0 0.6911409386785673 0.0 60-61 0.0 0.0 0.0 0.8018535376696623 0.0 62-63 0.0 0.0 0.0 0.9130794871970376 0.0 64-65 0.0 0.0 0.0 1.0441549907939138 0.0 66-67 0.0 0.0 0.0 1.208256045558149 0.0 68-69 0.0 0.0 0.0 1.3706459318681168 0.0 70-71 0.0 0.0 0.0 1.5530564890930123 0.0 72-73 0.0 0.0 0.0 1.7416272527532701 0.0 74-75 0.0 0.0 0.0 1.9748595130699047 0.0 76-77 0.0 0.0 0.0 2.2270857432289066 0.0 78-79 0.0 0.0 0.0 2.4878678156592446 0.0 80-81 0.0 0.0 0.0 2.7936536184368133 0.0 82-83 0.0 0.0 0.0 3.1098775487854127 0.0 84-85 0.0 0.0 0.0 3.4213102074620636 0.0 86-87 0.0 0.0 0.0 3.795713865255751 0.0 88-89 0.0 0.0 0.0 4.1530058385067665 0.0 90-91 0.0 0.0 0.0 4.554103724187024 0.0 92-93 0.0 0.0 0.0 4.9800135524541576 0.0 94-95 0.0 0.0 0.0 5.4545205648224835 0.0 96-97 0.0 0.0 0.0 5.944770326970069 0.0 98-99 0.0 0.0 0.0 6.475403664638362 0.0 100-101 0.0 0.0 0.0 7.038035852401453 0.0 102-103 0.0 0.0 0.0 7.615897439407525 0.0 104-105 0.0 0.0 0.0 8.232944784016318 0.0 106-107 0.0 0.0 0.0 8.848452077016269 0.0 108-109 0.0 0.0 0.0 9.54677992320276 0.0 110-111 0.0 0.0 0.0 10.207290946549943 0.0 112-113 0.0 0.0 0.0 10.93967104497635 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1585 0.0 91.63944 1 TCAGCCG 25 0.0020528575 71.42147 5 GAGGTAC 25 0.0020556478 71.397026 6 ATATAGG 110 3.6379788E-12 54.107174 2 GTATCAA 2715 0.0 53.489376 2 AACCGCA 35 0.0077917534 50.99787 9 CAGCCGA 35 0.0077917534 50.99787 6 GCCTATC 85 6.182199E-8 48.99796 7 TGTTACG 25 5.3315336E-4 47.598015 36-37 GTACATG 2120 0.0 44.084476 1 GCTTACT 140 4.0017767E-11 42.498226 8 ACATGGG 2150 0.0 42.35459 3 AGGCTTA 155 1.8189894E-12 42.224045 6 CCACGCA 85 3.4647765E-6 42.01263 4 ATGGGAT 650 0.0 41.204693 5 GTGGTAT 365 0.0 40.77266 1 CATGGGT 210 0.0 39.678593 4 GGTACTC 60 0.0013049808 39.678593 3 GACTTGA 120 2.0676453E-8 39.665012 7 CCAACGT 30 0.0013082528 39.665012 14-15 >>END_MODULE