FastQCFastQC Report
Thu 26 May 2016
SRR936199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48555
Sequences flagged as poor quality0
Sequence length125
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19393.99340953557821No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13382.7556379363608277No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9501.9565441252188238No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8011.64967562557924No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.9741530223457935No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.7620224487694368No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.5869632375656472No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.48810627123880135No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.30274945937596537No Hit
CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC1470.30274945937596537No Hit
AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.22036865410359385No Hit
GGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGG1010.2080115333127381No Hit
AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.20595201318092884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.18329729173102668No Hit
GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.1791782514674081No Hit
ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.16682113067655235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.16682113067655235No Hit
GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.16476161054474306No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.13592832869941301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.1256307280403666No Hit
GGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGG600.1235712079085573No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG550.11327360724951087No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA550.11327360724951087No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.10915456698589229No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT510.10503552672227372No Hit
AAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.10297600659046442No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.10297600659046442No Hit
GAGTTAAGTTATACTGTTACACTGACAAATCATCTTGTTGTGAGGATGCA490.10091648645865513No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATTG152.6866092E-4118.992797
TTCACCC208.434406E-489.24463
GTCCAAG208.434406E-489.24461
CCATTGG208.434406E-489.24468
ACATGGA358.832376E-784.994853
GAACAAA3100.074.85031
CCCCTTG250.002045456871.3956769
CCCTATA250.002045456871.3956767
ACCAAGC250.002045456871.3956767
CCTATAC250.002045456871.3956768
GAAAACA2700.068.310681
GTACAAG453.933088E-666.10711
GCCCATA201.7644014E-459.49639554-55
TCACCCA300.00421319859.4963954
CTGTAGA300.00421319859.4963951
GTACAAA401.7555697E-459.4963951
ATAGGCC150.0042259159.49639558-59
TAGACCA401.7555697E-459.4963954
ATAGACT300.00421319859.4963954
CCTGTGT300.00421319859.4963958