##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936199_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48555 Sequences flagged as poor quality 0 Sequence length 125 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.795633817320564 30.0 12.0 31.0 7.0 34.0 2 24.71891669241067 30.0 16.0 31.0 7.0 34.0 3 25.25130264648337 30.0 22.0 33.0 7.0 34.0 4 27.36838636597673 35.0 19.0 37.0 7.0 37.0 5 29.80624034599938 35.0 30.0 37.0 7.0 37.0 6 27.888847698486252 35.0 22.0 37.0 8.0 37.0 7 28.32872000823808 35.0 25.0 37.0 8.0 37.0 8 27.614190093708167 35.0 19.0 37.0 7.0 37.0 9 27.516692410668313 35.0 17.0 39.0 7.0 39.0 10-11 28.325568942436412 35.0 17.5 39.0 7.0 39.0 12-13 29.221151271753683 35.0 24.5 39.0 7.0 39.0 14-15 30.39299763155185 36.0 27.0 40.0 7.0 41.0 16-17 30.473669035114817 36.0 27.5 40.0 7.0 41.0 18-19 30.366316548244257 35.5 28.0 40.0 2.0 41.0 20-21 30.17257748944496 35.0 28.0 40.0 2.0 41.0 22-23 29.48285449490269 34.5 26.5 40.0 2.0 41.0 24-25 28.86173411595098 34.0 25.0 40.0 2.0 41.0 26-27 28.456502934816186 34.0 25.0 40.0 2.0 41.0 28-29 28.053629904232317 34.0 25.0 40.0 2.0 41.0 30-31 27.669117495623517 34.0 23.0 40.0 2.0 41.0 32-33 27.24695705900525 33.0 18.0 40.0 2.0 41.0 34-35 26.93139738440943 33.0 12.5 40.0 2.0 41.0 36-37 26.567356605910824 33.0 2.0 40.0 2.0 41.0 38-39 26.196334054165376 33.0 2.0 40.0 2.0 41.0 40-41 26.146812892596024 33.0 2.0 40.0 2.0 41.0 42-43 25.87007517248481 33.0 2.0 40.0 2.0 41.0 44-45 25.773874987128 33.0 2.0 40.0 2.0 41.0 46-47 25.75946864380599 33.0 2.0 40.0 2.0 41.0 48-49 25.614766759345073 33.0 2.0 40.0 2.0 41.0 50-51 25.463031613634023 33.0 2.0 40.0 2.0 41.0 52-53 25.366028215425807 33.0 2.0 40.0 2.0 41.0 54-55 25.259231798990832 33.0 2.0 39.5 2.0 41.0 56-57 25.027576974564926 32.0 2.0 39.0 2.0 41.0 58-59 24.830882504376483 32.0 2.0 39.0 2.0 41.0 60-61 24.578292657810728 31.5 2.0 39.0 2.0 41.0 62-63 24.239944392956442 31.0 2.0 39.0 2.0 41.0 64-65 23.95819174132427 31.0 2.0 38.5 2.0 41.0 66-67 23.65939656060138 31.0 2.0 38.0 2.0 40.0 68-69 23.32289156626506 30.5 2.0 38.0 2.0 40.0 70-71 22.978920811450934 30.0 2.0 37.0 2.0 40.0 72-73 22.62827721140974 30.0 2.0 37.0 2.0 40.0 74-75 22.356575018020802 30.0 2.0 36.0 2.0 39.0 76-77 22.076655339305944 30.0 2.0 36.0 2.0 39.0 78-79 21.79059829059829 30.0 2.0 35.5 2.0 39.0 80-81 21.502934816187828 29.0 2.0 35.0 2.0 37.5 82-83 21.261363402327255 29.0 2.0 35.0 2.0 37.0 84-85 21.13544434146844 29.0 2.0 35.0 2.0 37.0 86-87 20.950447945628667 29.0 2.0 35.0 2.0 36.0 88-89 20.747966223869838 29.0 2.0 35.0 2.0 36.0 90-91 20.559046442178975 29.0 2.0 35.0 2.0 36.0 92-93 20.357512099680775 29.0 2.0 35.0 2.0 35.5 94-95 20.219246215631756 28.5 2.0 35.0 2.0 35.0 96-97 20.044794562866855 28.0 2.0 35.0 2.0 35.0 98-99 19.923025435073626 28.0 2.0 35.0 2.0 35.0 100-101 19.817094017094014 28.0 2.0 35.0 2.0 35.0 102-103 19.12671197610957 26.5 2.0 34.0 2.0 35.0 104-105 19.503418803418803 27.0 2.0 34.5 2.0 35.0 106-107 19.526619297703636 27.5 2.0 35.0 2.0 35.0 108-109 19.443157244362062 27.0 2.0 35.0 2.0 35.0 110-111 19.307290701266602 27.0 2.0 35.0 2.0 35.0 112-113 19.259561322211923 27.0 2.0 35.0 2.0 35.0 114-115 19.1326125012872 26.0 2.0 35.0 2.0 35.0 116-117 18.975522603233447 25.5 2.0 35.0 2.0 35.0 118-119 18.794501081248068 25.0 2.0 35.0 2.0 35.0 120-121 18.63293172690763 25.0 2.0 34.0 2.0 35.0 122-123 18.355380496344353 25.0 2.0 34.0 2.0 35.0 124-125 17.188662341674387 21.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.763430444578283 1101 2 1.4687867941047195 1101 3 1.9406950610849556 1101 4 2.788554994817968 1101 5 2.535323461539143 1101 6 0.7259243900826142 1101 7 0.5461306041564349 1101 8 1.5221977286042865 1101 9 2.054489391251469 1101 10-11 2.318140244911689 1101 12-13 1.5470579961352406 1101 14-15 1.0910543449224122 1101 16-17 1.5688748506706744 1101 18-19 1.4188732983526293 1101 20-21 1.3797569271023171 1101 22-23 1.3606045134679157 1101 24-25 2.398825336806773 1101 26-27 2.575028070750019 1101 28-29 2.952958448594842 1101 30-31 2.6113252475131183 1101 32-33 2.5267551671918262 1101 34-35 3.1014569724520236 1101 36-37 2.1316570192774478 1101 38-39 2.3597816770282165 1101 40-41 2.8829881568902103 1101 42-43 2.9131415561743097 1101 44-45 2.603123394045621 1101 46-47 3.0928266482182885 1101 48-49 2.615957415935135 1101 50-51 2.871925736505066 1101 52-53 3.040323763216275 1101 54-55 3.1591536391213637 1101 56-57 3.2280816834649286 1101 58-59 2.8550755968571018 1101 60-61 3.3130898776935496 1101 62-63 2.7843209646682183 1101 64-65 3.4213491181718325 1101 66-67 3.412948917636026 1101 68-69 3.1554548695972997 1101 70-71 2.6476948224470895 1101 72-73 2.270797488710592 1101 74-75 2.498457965270987 1101 76-77 2.5203927070549064 1101 78-79 2.499620200551213 1101 80-81 2.1186777688143152 1101 82-83 1.6148951489357088 1101 84-85 1.5480211266535946 1101 86-87 2.1098925414920053 1101 88-89 1.9740019845571588 1101 90-91 2.030833778049619 1101 92-93 2.2816209323450742 1101 94-95 2.1051380110903963 1101 96-97 2.0053730040551336 1101 98-99 2.0760265587875324 1101 100-101 2.328271653601366 1101 102-103 2.3110636996775007 1101 104-105 2.2164303159575063 1101 106-107 1.7346182665844196 1101 108-109 1.8076437036418653 1101 110-111 2.0476123350301805 1101 112-113 2.275961926714359 1101 114-115 2.392460833749592 1101 116-117 2.530288256256238 1101 118-119 2.489277338716704 1101 120-121 2.5758806501881715 1101 122-123 2.6454737254032246 1101 124-125 2.1719546386407522 1102 1 -0.08340499845969163 1102 2 0.08967287005408409 1102 3 0.5232899977938175 1102 4 0.3592828429192352 1102 5 -0.7069233739038943 1102 6 -0.1728097871325751 1102 7 -0.554976990780272 1102 8 -0.48207442329444916 1102 9 -0.3926625074827079 1102 10-11 -0.6988692487591983 1102 12-13 -0.25167618107994727 1102 14-15 -0.06733173102695744 1102 16-17 -0.3654605923672989 1102 18-19 0.1501232983526286 1102 20-21 0.251196800520038 1102 22-23 0.48892729827803905 1102 24-25 1.0648063494650053 1102 26-27 0.08127807075002025 1102 28-29 -0.35281686786085587 1102 30-31 0.08822398169033363 1102 32-33 0.37865390136904153 1102 34-35 0.3994000104267066 1102 36-37 0.2780177787711189 1102 38-39 0.5333576263953077 1102 40-41 0.6381305619534992 1102 42-43 0.5644864928831694 1102 44-45 0.5535980775899283 1102 46-47 1.0193298127752506 1102 48-49 0.688346656441464 1102 50-51 0.46694155928987513 1102 52-53 0.6292478138491866 1102 54-55 0.16121060114667785 1102 56-57 0.3763411771358136 1102 58-59 0.22224331837608702 1102 60-61 0.16657089035177464 1102 62-63 -0.24590055431912106 1102 64-65 -0.12683126157500624 1102 66-67 -0.37629158869308554 1102 68-69 -0.39486158609890154 1102 70-71 0.27024229080152296 1102 72-73 0.8461930583308437 1102 74-75 0.5367491045114932 1102 76-77 0.4067059981941483 1102 78-79 0.6149683018170329 1102 80-81 0.5420954903332991 1102 82-83 0.11758502235343116 1102 84-85 0.5535591013371359 1102 86-87 0.822708997188208 1102 88-89 0.9602361617723467 1102 90-91 0.6385078286825276 1102 92-93 0.700450046269129 1102 94-95 0.7966728212169762 1102 96-97 1.0001514850677928 1102 98-99 1.1590170651166467 1102 100-101 1.203271653601366 1102 102-103 1.1128833199306634 1102 104-105 1.2393733539321907 1102 106-107 1.4822448488628979 1102 108-109 1.2928493998443997 1102 110-111 1.643893980599799 1102 112-113 1.7444745849422034 1102 114-115 1.4167488084331339 1102 116-117 0.8927882562562388 1102 118-119 0.8363501235268309 1102 120-121 0.7743774856312093 1102 122-123 0.6834484089475303 1102 124-125 -0.46340928540987747 1103 1 -2.28603657740706 1103 2 -0.7155902878406515 1103 3 -0.951710002206184 1103 4 -2.3032171570807662 1103 5 -1.2523181107459997 1103 6 -2.1333361029220477 1103 7 -2.555634885517115 1103 8 -1.8064165285576053 1103 9 -2.888715139061656 1103 10-11 -3.305777143496041 1103 12-13 -2.5095709179220513 1103 14-15 -2.060094888921693 1103 16-17 -2.0134869081567714 1103 18-19 -1.2258635437526308 1103 20-21 -1.5168953047431195 1103 22-23 -1.7485727017219581 1103 24-25 -1.1253252294823675 1103 26-27 -2.1420771924078785 1103 28-29 -2.0024879204924346 1103 30-31 -1.9867760183096657 1103 32-33 -1.9331882038941153 1103 34-35 -2.3788894632575044 1103 36-37 -2.0410611685973024 1103 38-39 -1.1225634262362725 1103 40-41 -1.3437773327833433 1103 42-43 -1.0384740334326175 1103 44-45 -1.3194282381995457 1103 46-47 -0.9777096609089604 1103 48-49 -1.6323770277690635 1103 50-51 -1.987334756499596 1103 52-53 -2.1154890282560785 1103 54-55 -1.6368157146427933 1103 56-57 -1.582211454443133 1103 58-59 -2.508348786887069 1103 60-61 -2.6123764780692795 1103 62-63 -2.639979501687545 1103 64-65 -2.7225549457855323 1103 66-67 -2.699975799219402 1103 68-69 -2.422493165046273 1103 70-71 -3.114297182882691 1103 72-73 -2.862030625879683 1103 74-75 -2.5435140533832445 1103 76-77 -2.4429650544374297 1103 78-79 -2.5225316981829664 1103 80-81 -2.310536088614068 1103 82-83 -1.9264939250149915 1103 84-85 -1.910256688136549 1103 86-87 -1.8253173186012646 1103 88-89 -2.725948048753967 1103 90-91 -2.4144526976332585 1103 92-93 -2.580471006362451 1103 94-95 -2.5918140208882825 1103 96-97 -2.4057695675637873 1103 98-99 -2.2847329348833547 1103 100-101 -1.8822546621881067 1103 102-103 -1.5170508905956517 1103 104-105 -1.731021382909912 1103 106-107 -1.8499919932423623 1103 108-109 -1.652216389629288 1103 110-111 -2.2630139141370407 1103 112-113 -2.0982885729525336 1103 114-115 -2.1908169810405482 1103 116-117 -1.7789222700595495 1103 118-119 -1.9027946133152724 1103 120-121 -2.6759514617372133 1103 122-123 -2.5665515910524697 1103 124-125 -2.084790864357249 1104 1 0.016595001540309795 1104 2 0.7530062033874181 1104 3 -0.06504333553951724 1104 4 0.6867828429192322 1104 5 0.7422432927627725 1104 6 0.013856879534092315 1104 7 1.2308563425530608 1104 8 -0.28624108996111275 1104 9 0.0823374925172935 1104 10-11 -0.6026192487592006 1104 12-13 0.6616571522533832 1104 14-15 -0.20649839769362188 1104 16-17 0.2287060742993674 1104 18-19 -0.1811267016473721 1104 20-21 0.3416134671867077 1104 22-23 0.7664272982780425 1104 24-25 0.9639730161316713 1104 26-27 0.7216947374166871 1104 28-29 0.9309331321391419 1104 30-31 0.16322398169033292 1104 32-33 0.5586539013690413 1104 34-35 -0.023099989573292845 1104 36-37 -1.5794822212288828 1104 38-39 -1.1741423736046954 1104 40-41 -1.8922861047131683 1104 42-43 -1.365930173783493 1104 44-45 -0.9989019224100737 1104 46-47 -1.5335868538914177 1104 48-49 -1.1062366768918714 1104 50-51 -1.2734751073767931 1104 52-53 -1.0124188528174827 1104 54-55 -1.8296227321866532 1104 56-57 -1.7911588228641868 1104 58-59 -1.284840014957247 1104 60-61 -1.3217624429815586 1104 62-63 -1.5400672209857902 1104 64-65 -1.5864145949083408 1104 66-67 -1.7462915886930865 1104 68-69 -1.3965282527655667 1104 70-71 -1.2618410425318096 1104 72-73 -1.4908902750024886 1104 74-75 -1.1049175621551726 1104 76-77 -1.147877335139185 1104 78-79 -0.739198364849635 1104 80-81 -0.20790450966670093 1104 82-83 -0.5432483109799051 1104 84-85 -1.0756075653295305 1104 86-87 -1.2581243361451229 1104 88-89 -1.5005971715609867 1104 90-91 -1.0885755046508052 1104 92-93 -0.525383287064205 1104 94-95 -0.48541051211635633 1104 96-97 -1.0190151815988777 1104 98-99 -0.7380662682166879 1104 100-101 -0.9717283463986348 1104 102-103 -1.1967000134026726 1104 104-105 -1.3214599794011441 1104 106-107 -1.2885884844704378 1104 108-109 -1.2759006001556017 1104 110-111 -0.9898560194002002 1104 112-113 -1.0163587483911272 1104 114-115 -0.39116785823352984 1104 116-117 -0.4563784104104265 1104 118-119 -0.1648998764731715 1104 120-121 -0.08437251436879123 1104 122-123 -0.6032182577191385 1104 124-125 -0.39049261874321317 1105 1 -0.3804638219891032 1105 2 0.022025811230555803 1105 3 0.43064293897028705 1105 4 0.7409004899780562 1105 5 0.8026354496255195 1105 6 0.5036608011027184 1105 7 0.29575830333737585 1105 8 -0.20192736447091875 1105 9 0.7088080807525863 1105 10-11 0.18532192771138867 1105 12-13 0.11891205421416728 1105 14-15 1.0819329748553947 1105 16-17 0.2926276429268171 1105 18-19 0.05857918070557133 1105 20-21 -0.544759081832904 1105 22-23 -0.5132785840749001 1105 24-25 0.4493651729944155 1105 26-27 -0.29261898807351017 1105 28-29 -0.2737727502137979 1105 30-31 -0.3103054300743686 1105 32-33 0.2742421366631582 1105 34-35 0.4736647163090595 1105 36-37 1.3405177787711189 1105 38-39 1.2237988028658933 1105 40-41 1.1767335031299702 1105 42-43 1.1457364928831701 1105 44-45 1.545509842295811 1105 46-47 1.360138636304665 1105 48-49 1.3335672446767575 1105 50-51 0.9364268534075215 1105 52-53 1.0366007550256562 1105 54-55 1.4854753070290343 1105 56-57 1.5594294124299282 1105 58-59 1.5520227301407914 1105 60-61 2.172453243292953 1105 62-63 2.234246504504405 1105 64-65 1.7213305031308792 1105 66-67 1.9722378230716195 1105 68-69 0.821314884489329 1105 70-71 0.870609937860344 1105 72-73 1.375972470095551 1105 74-75 1.0323373398056077 1105 76-77 1.087220704076504 1105 78-79 0.9554094782876206 1105 80-81 1.117095490333302 1105 82-83 0.3742026694122558 1105 84-85 0.6895885131018424 1105 86-87 0.914620761894092 1105 88-89 1.5197949853017612 1105 90-91 1.5712284169178226 1105 92-93 1.1923618109750116 1105 94-95 1.1705698800405067 1105 96-97 1.4700044262442624 1105 98-99 1.4211494180578192 1105 100-101 1.9165069477190109 1105 102-103 1.7581039081659569 1105 104-105 2.0739321774616037 1105 106-107 1.5668036723923109 1105 108-109 2.256452341020868 1105 110-111 1.8604380982468598 1105 112-113 1.9187392908245613 1105 114-115 1.9825576319625497 1105 116-117 1.8464647268444736 1105 118-119 1.9536295352915367 1105 120-121 2.2791568973959144 1105 122-123 2.499624879535766 1105 124-125 3.188428949884237 1106 1 -0.6149839458281114 1106 2 -0.14980081415644264 1106 3 0.4693426293727647 1106 4 -0.3690066307649751 1106 5 -0.44968653179862983 1106 6 0.28771652865689745 1106 7 0.26015458816709724 1106 8 -1.0827323180312902 1106 9 -0.25713619169323465 1106 10-11 -0.3189350382328833 1106 12-13 0.43779750313057875 1106 14-15 0.2162209005519884 1106 16-17 0.8680920392116462 1106 18-19 0.3728206667736842 1106 20-21 0.37784153736214066 1106 22-23 -0.13015164909037935 1106 24-25 -2.5397989136928913 1106 26-27 -0.20128771872366613 1106 28-29 0.3988278689812468 1106 30-31 0.11190819221664938 1106 32-33 -3.2095039933678002 1106 34-35 0.04874211568986553 1106 36-37 0.3076230419290127 1106 38-39 0.45638394218477885 1106 40-41 -0.18588259594123713 1106 42-43 -0.4661056123799874 1106 44-45 -0.5957440276732306 1106 46-47 -0.8395517661721179 1106 48-49 -0.7113243961901148 1106 50-51 -0.09259791439433585 1106 52-53 -0.32601534404555466 1106 54-55 0.02107902219930935 1106 56-57 -0.32563250707471525 1106 58-59 -0.8241382605712815 1106 60-61 -0.1321133201745397 1106 62-63 0.2547573404177186 1106 64-65 0.060339791056573944 1106 66-67 -0.1473442202720321 1106 68-69 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-0.7933666800693366 2216 104-105 -0.8362845408046518 2216 106-107 -0.9881498879792083 2216 108-109 -1.2311637580503394 2216 110-111 -0.9077507562423008 2216 112-113 -0.9930254150577937 2216 114-115 -1.2960801389352845 2216 116-117 -1.0091854279542893 2216 118-119 -0.7975314554205397 2216 120-121 -1.1562146196319496 2216 122-123 -1.5665515910524697 2216 124-125 -1.1900540222519815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3466.0 3 731.0 4 1197.0 5 2075.0 6 3217.0 7 1415.0 8 653.0 9 616.0 10 648.0 11 607.0 12 656.0 13 618.0 14 616.0 15 478.0 16 411.0 17 345.0 18 328.0 19 296.0 20 278.0 21 288.0 22 298.0 23 572.0 24 992.0 25 725.0 26 1291.0 27 710.0 28 782.0 29 787.0 30 826.0 31 966.0 32 1247.0 33 1544.0 34 2049.0 35 2770.0 36 4178.0 37 6758.0 38 3101.0 39 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.35434043867779 25.71928740603439 9.391411801050355 15.534960354237462 2 14.406343322005974 40.506641952425085 27.589331685717227 17.497683039851715 3 15.038616002471425 48.81268664401194 21.79796107506951 14.35073627844712 4 8.215425805787252 32.07496653279786 26.306250643600045 33.40335701781485 5 7.368963031613634 58.729276078673664 24.54947997116672 9.35228091854598 6 18.838430645659564 49.22047163011018 15.355782102770055 16.5853156214602 7 18.787329571199077 52.33760349301808 16.379700951517897 12.495365984264941 8 17.45685216460024 53.11199901140997 15.378753552745398 14.05239527124439 9 19.87477859702599 36.78378712361494 12.806359929151048 30.535074350208014 10-11 16.1191662890802 47.822012604522804 20.382460765333445 15.676360341063559 12-13 17.54971320296168 50.02522990103701 19.291091270454242 13.133965625547079 14-15 17.14912416200686 49.92842946440524 18.0141493404183 14.908297033169596 16-17 15.819825757419729 50.78573929520318 19.28511111568801 14.109323831689084 18-19 16.329577986942105 48.50575659588491 21.02547731345128 14.139188103721706 20-21 19.204786521945092 46.67888698947542 20.176920066731203 13.939406421848288 22-23 17.87473972828664 48.14150534974333 20.22697754963201 13.756777372338014 24-25 16.995510153643366 48.682909749969106 20.048811632409276 14.27276846397825 26-27 16.60951321737877 48.38271185393432 20.512424439020872 14.49535048966604 28-29 16.3024673559336 48.849734316431196 20.979733904518678 13.86806442311653 30-31 16.74819017804735 49.48048069694879 20.36989362468978 13.40143550031408 32-33 15.833916876055525 49.694154961486184 20.13119413436586 14.340734028092433 34-35 15.659883840672242 50.49841413683733 20.488528236602548 13.35317378588788 36-37 16.182846080177946 49.52990968911223 19.77159686537808 14.515647365331741 38-39 15.159410141285992 50.37689994645137 20.384520327882356 14.079169584380278 40-41 15.718581373316306 50.827944144663675 20.069407257898423 13.384067224121596 42-43 15.53100124602251 50.64309178345982 20.36783408334964 13.458072887168026 44-45 15.750342398747799 50.18072475259759 20.028009762225953 14.040923086428652 46-47 15.10277217119084 50.40058491576389 20.77686699345059 13.719775919594678 48-49 14.92137700934002 49.629797444109194 20.126867746552843 15.32195779999794 50-51 15.196482267166452 49.642665897763315 20.31140585739589 14.84944597767434 52-53 15.196482267166452 49.05260122749928 20.384520327882356 15.36639617745191 54-55 15.363306833628537 48.80751328417844 19.89846356633851 15.930716315854513 56-57 15.512625118424847 48.205091238620916 19.273386332742927 17.00889731021131 58-59 15.490680671403561 48.922870971063745 19.77345278550098 15.812995572031715 60-61 16.478559953865798 48.007373233925115 19.732668781150885 15.781398031058202 62-63 16.170655352803067 48.03311776578655 19.619392840960582 16.17683404044981 64-65 15.869959220661531 48.580961403797836 20.252708324751822 15.296371050788812 66-67 16.265395230053137 48.79824525270832 20.263006137496394 14.673353379742144 68-69 17.010606528678817 48.824014004736895 19.305941715580268 14.859437751004014 70-71 16.459684893419833 49.69725054062403 19.96189887756153 13.881165688394603 72-73 16.94470188446092 48.780764081968904 19.99794047986819 14.27659355370199 74-75 17.282463186077642 48.520234785295024 20.27700545772835 13.92029657089898 76-77 17.1836062197508 47.204201421068895 20.91133765832561 14.700854700854702 78-79 17.812789620018535 46.88188652044074 20.906188857996085 14.399135001544641 80-81 18.007414272474513 45.74812068787972 20.50355267222737 15.740912367418392 82-83 17.97343219029966 45.827412212954385 21.259396560601378 14.93975903614458 84-85 18.821954484605087 44.900628153640206 20.81351045206467 15.463906909690042 86-87 18.16187828236021 44.9232828750901 21.006075584388835 15.90876325816085 88-89 18.063021316033364 44.94284831634229 21.36855112758727 15.625579240037071 90-91 18.39666357738647 44.909895994233345 21.358253526928227 15.33518690145196 92-93 18.240140047368964 45.03861600247143 20.61888579960869 16.10235815055092 94-95 17.331891669241067 45.78210277005458 21.249098959942334 15.636906600762021 96-97 17.009576768612913 46.029245185871694 20.904129337864276 16.057048707651116 98-99 16.253732880238907 47.42148079497477 20.40057666563691 15.924209659149419 100-101 15.244568015652352 48.660282154258056 20.695088044485637 15.400061785603954 102-103 14.39707548141283 50.92472453918238 19.862012151168777 14.816187828236021 104-105 14.263353551164156 51.35363354580935 19.223758868899896 15.1592540341266 106-107 13.814231284110804 52.186180619915554 19.522191329420245 14.477396766553394 108-109 14.220986510143135 52.605292966738745 18.500669344042837 14.673051179075275 110-111 13.358185132171066 53.27518561616328 19.10945432452193 14.257174927143726 112-113 13.648439913500155 52.82669138090825 19.343013077952836 14.18185562763876 114-115 13.725671918443002 52.91010194624653 18.953763773040883 14.410462362269591 116-117 14.036659458346204 52.51776336113686 18.689115436103386 14.756461744413551 118-119 14.215837709813615 51.73617547111523 19.39656060137988 14.651426217691277 120-121 14.881062712388013 51.08227782926578 19.440840284213778 14.595819174132426 122-123 14.719390382040984 50.30789825970549 19.471733086190916 15.50097827206261 124-125 14.967562557924003 50.97930182267532 19.224590670373804 14.828544949026876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1335.0 1 1573.0 2 1848.5 3 1481.0 4 872.5 5 546.0 6 356.0 7 306.5 8 306.0 9 281.0 10 255.0 11 210.5 12 160.5 13 152.5 14 161.5 15 155.0 16 148.0 17 150.5 18 166.0 19 181.0 20 207.0 21 249.5 22 278.0 23 316.5 24 415.0 25 537.5 26 688.0 27 780.0 28 847.0 29 932.0 30 1011.5 31 1212.0 32 1397.5 33 1565.0 34 1942.5 35 2048.0 36 1861.5 37 1741.5 38 1672.0 39 1614.0 40 1430.5 41 1459.0 42 1488.5 43 1347.0 44 1222.0 45 1034.5 46 895.5 47 847.5 48 801.5 49 744.0 50 787.5 51 697.5 52 567.5 53 548.0 54 483.0 55 453.5 56 414.5 57 348.5 58 325.5 59 262.5 60 161.5 61 124.0 62 96.5 63 86.5 64 85.5 65 65.5 66 54.5 67 46.5 68 38.5 69 37.5 70 45.0 71 41.0 72 31.5 73 28.0 74 22.0 75 21.0 76 22.5 77 15.5 78 12.5 79 13.0 80 9.5 81 8.5 82 9.5 83 6.5 84 4.0 85 3.5 86 3.0 87 2.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 2.0 94 2.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0020595201318092883 8 0.0020595201318092883 9 0.0020595201318092883 10-11 0.0020595201318092883 12-13 0.0030892801977139327 14-15 0.0030892801977139327 16-17 0.004119040263618577 18-19 0.004119040263618577 20-21 0.004119040263618577 22-23 0.09885696632684585 24-25 0.0020595201318092883 26-27 0.0030892801977139327 28-29 0.0020595201318092883 30-31 0.0010297600659046442 32-33 0.0020595201318092883 34-35 0.0020595201318092883 36-37 0.0010297600659046442 38-39 0.0020595201318092883 40-41 0.0020595201318092883 42-43 0.0010297600659046442 44-45 0.0010297600659046442 46-47 0.0020595201318092883 48-49 0.0010297600659046442 50-51 0.0020595201318092883 52-53 0.0020595201318092883 54-55 0.0020595201318092883 56-57 0.0020595201318092883 58-59 0.0 60-61 0.0020595201318092883 62-63 0.0020595201318092883 64-65 0.0020595201318092883 66-67 0.0020595201318092883 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0010297600659046442 106-107 0.0 108-109 0.0 110-111 0.0010297600659046442 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 48555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.44537122850375 #Duplication Level Percentage of deduplicated Percentage of total 1 74.18199945617692 33.71228503758624 2 9.195141847185715 8.357532694882092 3 5.374784736698994 7.327772628977448 4 3.657210187618961 6.648130985480383 5 2.30671621499139 5.241478735454639 6 1.5906824979606635 4.337349397590361 7 1.0060726910178555 3.2004942848316342 8 0.6571195504395902 2.3890433528987747 9 0.4305266020121453 1.7608897126969416 >10 1.4864497416840388 10.931932859643704 >50 0.05891416659113568 1.892699001132736 >100 0.03625487174839119 3.8451240860879414 >500 0.009063717937097798 3.606219750798064 >1k 0.009063717937097798 6.749047471939037 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1939 3.99340953557821 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1338 2.7556379363608277 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 950 1.9565441252188238 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 801 1.64967562557924 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 473 0.9741530223457935 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 370 0.7620224487694368 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 285 0.5869632375656472 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 237 0.48810627123880135 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 147 0.30274945937596537 No Hit CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC 147 0.30274945937596537 No Hit AAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.22036865410359385 No Hit GGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGG 101 0.2080115333127381 No Hit AAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.20595201318092884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.18329729173102668 No Hit GAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.1791782514674081 No Hit ACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.16682113067655235 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81 0.16682113067655235 No Hit GAAAACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.16476161054474306 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.13592832869941301 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.1256307280403666 No Hit GGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGG 60 0.1235712079085573 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 55 0.11327360724951087 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 55 0.11327360724951087 No Hit AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.10915456698589229 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 51 0.10503552672227372 No Hit AAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.10297600659046442 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.10297600659046442 No Hit GAGTTAAGTTATACTGTTACACTGACAAATCATCTTGTTGTGAGGATGCA 49 0.10091648645865513 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0010297600659046442 0.0 20-21 0.0 0.0 0.0 0.0020595201318092883 0.0 22-23 0.0 0.0 0.0 0.004119040263618577 0.0 24-25 0.0 0.0 0.0 0.010297600659046443 0.0 26-27 0.0 0.0 0.0 0.010297600659046443 0.0 28-29 0.0 0.0 0.0 0.013386880856760375 0.0 30-31 0.0 0.0 0.0 0.023684481515806814 0.0 32-33 0.0 0.0 0.0 0.054577283492946146 0.0 34-35 0.0 0.0 0.0 0.08341056533827618 0.0 36-37 0.0 0.0 0.0 0.11327360724951085 0.0 38-39 0.0 0.0 0.0 0.1812377715992174 0.0 40-41 0.0 0.0 0.0 0.2471424158171146 0.0 42-43 0.0 0.0 0.0 0.29863041911234683 0.0 44-45 0.0 0.0 0.0 0.3779219441870044 0.0 46-47 0.0 0.0 0.0 0.4530944289980434 0.0 48-49 0.0 0.0 0.0 0.5159097930182268 0.0 50-51 0.0 0.0 0.0 0.5982905982905983 0.0 52-53 0.0 0.0 0.0 0.6621357223766862 0.0 54-55 0.0 0.0 0.0 0.7548141283081042 0.0 56-57 0.0 0.0 0.0 0.8722067758212337 0.0 58-59 0.0 0.0 0.0 0.9823911028730306 0.0 60-61 0.0 0.0 0.0 1.074039748738544 0.0 62-63 0.0 0.0 0.0 1.1996704767789104 0.0 64-65 0.0 0.0 0.0 1.3211821645556585 0.0 66-67 0.0 0.0 0.0 1.4550509731232624 0.0 68-69 0.0 0.0 0.0 1.59200906188858 0.0 70-71 0.0 0.0 0.0 1.7711873133559881 0.0 72-73 0.0 0.0 0.0 1.9297703635053032 0.0 74-75 0.0 0.0 0.0 2.1110081351045205 0.0 76-77 0.0 0.0 0.0 2.321079188549068 0.0 78-79 0.0 0.0 0.0 2.535269282257234 0.0 80-81 0.0 0.0 0.0 2.7772628977448255 0.0 82-83 0.0 0.0 0.0 3.0635361960663166 0.0 84-85 0.0 0.0 0.0 3.3178869323447637 0.0 86-87 0.0 0.0 0.0 3.571207908557306 0.0 88-89 0.0 0.0 0.0 3.8482133662856555 0.0 90-91 0.0 0.0 0.0 4.213778189681804 0.0 92-93 0.0 0.0 0.0 4.539182370507671 0.0 94-95 0.0 0.0 0.0 4.882092472453918 0.0 96-97 0.0 0.0 0.0 5.234270414993307 0.0 98-99 0.0 0.0 0.0 5.579240037071362 0.0 100-101 0.0 0.0 0.0 5.9726083822469365 0.0 102-103 0.0 0.0 0.0 6.3659767274225105 0.0 104-105 0.0 0.0 0.0 6.764493872927607 0.0 106-107 0.0 0.0 0.0 7.1990526207393675 0.0 108-109 0.0 0.0 0.0 7.631551848419319 0.0 110-111 0.0 0.0 0.0 8.073318916692411 0.0 112-113 0.0 0.0 0.0 8.465657501802081 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCATTG 15 2.6866092E-4 118.99279 7 TTCACCC 20 8.434406E-4 89.2446 3 GTCCAAG 20 8.434406E-4 89.2446 1 CCATTGG 20 8.434406E-4 89.2446 8 ACATGGA 35 8.832376E-7 84.99485 3 GAACAAA 310 0.0 74.8503 1 CCCCTTG 25 0.0020454568 71.395676 9 CCCTATA 25 0.0020454568 71.395676 7 ACCAAGC 25 0.0020454568 71.395676 7 CCTATAC 25 0.0020454568 71.395676 8 GAAAACA 270 0.0 68.31068 1 GTACAAG 45 3.933088E-6 66.1071 1 GCCCATA 20 1.7644014E-4 59.496395 54-55 TCACCCA 30 0.004213198 59.496395 4 CTGTAGA 30 0.004213198 59.496395 1 GTACAAA 40 1.7555697E-4 59.496395 1 ATAGGCC 15 0.00422591 59.496395 58-59 TAGACCA 40 1.7555697E-4 59.496395 4 ATAGACT 30 0.004213198 59.496395 4 CCTGTGT 30 0.004213198 59.496395 8 >>END_MODULE