FastQCFastQC Report
Thu 26 May 2016
SRR936199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48555
Sequences flagged as poor quality0
Sequence length125
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28145.795489650911337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24024.946967356605911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22424.617444135516425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7211.484914015034497No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4961.021521985377407No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.9741530223457935No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.757903408505818No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.7414272474513438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.45721346926166206No Hit
CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC1820.3748326639892905No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.28215425805787253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1250.25744001647616105No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.2306662547626403No Hit
GGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGG1040.214190093708166No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.20389249304911955No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT860.1771187313355988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC800.16476161054474306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG780.1606425702811245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC770.1585830501493152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC660.13592832869941301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC650.13386880856760375No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA620.12769024817217586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC610.1256307280403666No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT600.1235712079085573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC570.11739264751312944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC570.11739264751312944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC560.11533312738132015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT530.10915456698589229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC520.107095046854083No Hit
GTTAAGAGAAGAATGGCCTCTATTAGAAAACTTGAAAGTGATTGGTAAGG490.10091648645865513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.087.11921
CTGTAAA300.004162950459.676651
CTTAACC201.7650056E-459.49227532-33
ACCGCTG150.004227069759.49227536-37
ACATTGC150.004227069759.49227548-49
CCGCTGA150.004227069759.49227536-37
ACCTCAG300.00421435459.4922758
GCTCTGC150.004227069759.49227526-27
TAACCGC150.004227069759.49227534-35
GATGGAA150.004227069759.49227580-81
AGGCAAC150.004227069759.49227592-93
CCTCAGG300.00421435459.4922759
TTAACCG150.004227069759.49227532-33
ACATGCC201.7650056E-459.49227512-13
TAGGCTC150.004227069759.49227584-85
CCTATTC150.004227069759.49227564-65
GGCTCCA150.004227069759.49227586-87
GTTGCTT150.004227069759.49227512-13
CTTACAT150.004227069759.49227544-45
GTATCAA10650.056.0344162
Produced by FastQC (version 0.11.5)