##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936199_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48555 Sequences flagged as poor quality 0 Sequence length 125 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.366120893831738 31.0 31.0 34.0 30.0 34.0 2 31.158232931726907 31.0 31.0 34.0 28.0 34.0 3 31.316033364226136 31.0 31.0 34.0 28.0 34.0 4 35.354278653073834 37.0 35.0 37.0 33.0 37.0 5 35.077952836988985 37.0 35.0 37.0 33.0 37.0 6 35.33051179075275 37.0 35.0 37.0 33.0 37.0 7 35.152425084955205 37.0 35.0 37.0 32.0 37.0 8 35.267325713108846 37.0 35.0 37.0 32.0 37.0 9 36.8179796107507 39.0 37.0 39.0 32.0 39.0 10-11 36.76085881989496 39.0 37.0 39.0 32.0 39.0 12-13 36.650653897641845 39.0 36.0 39.0 32.0 39.0 14-15 37.34504170528267 39.0 36.0 41.0 32.0 41.0 16-17 37.35905673977963 39.0 36.5 41.0 32.0 41.0 18-19 37.86521470497374 40.0 37.0 41.0 32.5 41.0 20-21 38.00576665636906 40.0 37.0 41.0 33.0 41.0 22-23 37.79300792915251 40.0 36.5 41.0 32.5 41.0 24-25 37.58728246318608 40.0 36.0 41.0 32.0 41.0 26-27 37.17081659973226 40.0 35.5 41.0 32.0 41.0 28-29 36.8059829059829 39.5 35.0 41.0 31.0 41.0 30-31 35.98390485016991 37.5 35.0 40.0 30.5 41.0 32-33 34.974461950365566 36.0 34.5 40.0 29.5 41.0 34-35 34.34441355164247 35.0 33.5 40.0 27.0 41.0 36-37 33.77200082380805 35.0 33.0 40.0 23.5 41.0 38-39 33.430099886726396 35.0 33.0 40.0 21.0 41.0 40-41 33.03925445371229 35.0 33.0 40.0 16.5 41.0 42-43 32.48577901348986 35.0 33.0 40.0 8.5 41.0 44-45 31.71458140253321 35.0 32.5 40.0 2.0 41.0 46-47 30.51101843270518 35.0 30.0 40.0 2.0 41.0 48-49 29.529729173102666 35.0 24.5 40.0 2.0 41.0 50-51 29.15840799093811 35.0 24.0 40.0 2.0 41.0 52-53 28.968489341983318 35.0 23.5 40.0 2.0 41.0 54-55 28.73712285037586 35.0 23.0 40.0 2.0 41.0 56-57 28.474266295953043 35.0 21.5 40.0 2.0 41.0 58-59 28.23928534651426 35.0 20.5 40.0 2.0 41.0 60-61 28.003851302646485 35.0 20.0 39.5 2.0 41.0 62-63 27.74220986510143 35.0 19.0 39.0 2.0 41.0 64-65 27.480589022757698 35.0 14.0 39.0 2.0 41.0 66-67 27.23181958603645 35.0 9.5 39.0 2.0 41.0 68-69 26.893028524353824 35.0 4.0 38.0 2.0 41.0 70-71 26.5764390896921 35.0 2.0 38.0 2.0 40.0 72-73 26.20923694779116 34.5 2.0 37.0 2.0 40.0 74-75 25.941705282669137 34.0 2.0 37.0 2.0 40.0 76-77 25.608392544537125 34.0 2.0 36.5 2.0 39.0 78-79 25.24623622695912 34.0 2.0 36.0 2.0 39.0 80-81 24.8730202862733 34.0 2.0 36.0 2.0 38.5 82-83 24.534291010194625 34.0 2.0 35.0 2.0 37.0 84-85 24.17591391205849 33.0 2.0 35.0 2.0 37.0 86-87 23.88391514777057 33.0 2.0 35.0 2.0 36.5 88-89 23.61026670785707 33.0 2.0 35.0 2.0 36.0 90-91 23.310740397487386 33.0 2.0 35.0 2.0 36.0 92-93 23.04118010503553 33.0 2.0 35.0 2.0 36.0 94-95 22.77988878591288 33.0 2.0 35.0 2.0 35.0 96-97 22.52915250746576 33.0 2.0 35.0 2.0 35.0 98-99 22.318865204407373 33.0 2.0 35.0 2.0 35.0 100-101 22.104252909072187 32.0 2.0 35.0 2.0 35.0 102-103 21.175306353619607 30.0 2.0 34.0 2.0 35.0 104-105 21.56186798475955 31.5 2.0 35.0 2.0 35.0 106-107 21.502965708989805 32.0 2.0 35.0 2.0 35.0 108-109 21.30600350118422 32.0 2.0 35.0 2.0 35.0 110-111 21.12699001132736 32.0 2.0 35.0 2.0 35.0 112-113 20.92947173308619 31.0 2.0 35.0 2.0 35.0 114-115 20.761075069508806 31.0 2.0 35.0 2.0 35.0 116-117 20.513541344866645 31.0 2.0 35.0 2.0 35.0 118-119 20.309587066213574 31.0 2.0 35.0 2.0 35.0 120-121 20.020481927710843 30.5 2.0 35.0 2.0 35.0 122-123 19.749325507156833 30.0 2.0 35.0 2.0 35.0 124-125 18.485614251879312 25.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3576885791971627 1101 2 0.9178182806187749 1101 3 0.3987067395709367 1101 4 0.5591561357074895 1101 5 0.4902024503352891 1101 6 0.22450149890540416 1101 7 0.5574765573251952 1101 8 0.6822855387477702 1101 9 0.3728108715716729 1101 10-11 0.9604805669703964 1101 12-13 0.9616925322855181 1101 14-15 0.8787855404667582 1101 16-17 0.5167739394998705 1101 18-19 0.7674141227792077 1101 20-21 0.9078652854569995 1101 22-23 0.449177935982668 1101 24-25 0.9042115597299727 1101 26-27 0.6075047434398542 1101 28-29 1.2290715379677692 1101 30-31 1.2385170575643514 1101 32-33 0.9877769288517371 1101 34-35 -0.08943526525333567 1101 36-37 0.8074450473282369 1101 38-39 1.631348555735613 1101 40-41 1.4932273405064223 1101 42-43 1.6276270824265424 1101 44-45 1.878082554859553 1101 46-47 1.1982453330867173 1101 48-49 1.13965916303966 1101 50-51 1.229933778831434 1101 52-53 1.093166631112041 1101 54-55 1.4686677363913923 1101 56-57 1.2538885734664937 1101 58-59 1.3757008694647794 1101 60-61 1.6026765081326673 1101 62-63 1.5730205013011727 1101 64-65 1.3790496309669997 1101 66-67 1.5173993220087993 1101 68-69 1.4947125394951506 1101 70-71 1.4311193667729327 1101 72-73 2.51248323405267 1101 74-75 2.1278366513817524 1101 76-77 2.3729641853747836 1101 78-79 2.7043433626409445 1101 80-81 2.3135757454255277 1101 82-83 2.337552174998521 1101 84-85 2.317751393123274 1101 86-87 2.6227747748311465 1101 88-89 3.0639672033442302 1101 90-91 2.630445987614607 1101 92-93 3.043524708009759 1101 94-95 3.1331725899580896 1101 96-97 2.825957715331132 1101 98-99 2.84592243306939 1101 100-101 2.891404083775871 1101 102-103 3.2118428719977707 1101 104-105 2.2817802142903787 1101 106-107 2.531826871900769 1101 108-109 1.6157944802912994 1101 110-111 2.4651320419651768 1101 112-113 2.733170489340626 1101 114-115 2.1853723997233523 1101 116-117 1.7525290915638756 1101 118-119 1.9424077830297648 1101 120-121 2.108722215415586 1101 122-123 1.840145356792398 1101 124-125 2.057898537122977 1102 1 0.12272022476678401 1102 2 0.941710685682061 1102 3 0.014529524381064363 1102 4 0.34728904709989195 1102 5 0.6824492857783255 1102 6 0.31738124574084736 1102 7 0.4731411142872233 1102 8 -0.018347372644633708 1102 9 -0.09222077399795126 1102 10-11 0.2692622125400206 1102 12-13 0.19096468418425872 1102 14-15 0.4687380721123233 1102 16-17 0.7993688762087316 1102 18-19 0.9972400721462904 1102 20-21 0.8710773107734511 1102 22-23 0.8773424929446847 1102 24-25 1.1167906736540232 1102 26-27 1.622140819389223 1102 28-29 1.4971095126513205 1102 30-31 1.2484854119947357 1102 32-33 1.9191851566998395 1102 34-35 0.8554223296833712 1102 36-37 1.4985842878345608 1102 38-39 1.5533422266216874 1102 40-41 1.0583381000000998 1102 42-43 1.2341144241986939 1102 44-45 1.510361035872208 1102 46-47 2.215175712833556 1102 48-49 0.7701180237991565 1102 50-51 1.1333356775656114 1102 52-53 1.2317742260487492 1102 54-55 1.31218039461924 1102 56-57 0.6909138899221894 1102 58-59 1.001333780857184 1102 60-61 1.203942330917478 1102 62-63 1.4970711342125647 1102 64-65 1.1208217828657325 1102 66-67 0.8915290688442425 1102 68-69 0.46037709645717584 1102 70-71 0.614030759177993 1102 72-73 1.023796525191912 1102 74-75 0.7166024741665602 1102 76-77 1.2943249448684533 1102 78-79 1.3067958942865125 1102 80-81 1.1809808087166687 1102 82-83 2.0219667319605463 1102 84-85 2.3634001273004905 1102 86-87 2.181002622932411 1102 88-89 2.088413405875876 1102 90-91 2.4707149749563797 1102 92-93 1.9447114168705149 1102 94-95 2.2558782861606197 1102 96-97 1.9614007533058135 1102 98-99 2.4334224330693885 1102 100-101 3.104853450864475 1102 102-103 3.3207827454154923 1102 104-105 3.0350238851764537 1102 106-107 2.125497757976717 1102 108-109 1.7171394170001584 1102 110-111 1.6951162191803633 1102 112-113 1.8375217551634115 1102 114-115 1.6429673364322142 1102 116-117 1.9246018763740018 1102 118-119 1.414955251384196 1102 120-121 1.3335639875674836 1102 122-123 1.5289902935012556 1102 124-125 2.0533099295280373 1103 1 0.310220224766784 1103 2 0.4344738435768001 1103 3 0.7434768928021178 1103 4 0.6097890470998948 1103 5 0.6725808647256954 1103 6 0.4443549299513734 1103 7 0.5080095353398519 1103 8 0.6316526273553649 1103 9 0.11764764705468167 1103 10-11 -0.21198778745998226 1103 12-13 0.1587278420789957 1103 14-15 0.3118301773754837 1103 16-17 0.09805308673504953 1103 18-19 0.7850690195147152 1103 20-21 0.3332483634050263 1103 22-23 0.49839512452363266 1103 24-25 0.16054067365402602 1103 26-27 0.5152329246523806 1103 28-29 0.05368846001973537 1103 30-31 -0.22717248274211244 1103 32-33 -0.40088063277384833 1103 34-35 -1.4021434597903166 1103 36-37 -1.3027315016391228 1103 38-39 -1.7144209312730538 1103 40-41 -2.0084382157893756 1103 42-43 -1.7020697863276197 1103 44-45 -1.9475337009698954 1103 46-47 -2.4736400766401303 1103 48-49 -1.6407372393587423 1103 50-51 -1.5637037961185989 1103 52-53 -0.9366468265828303 1103 54-55 -0.9187406580123394 1103 56-57 -1.2709282153409696 1103 58-59 -2.6197188507217604 1103 60-61 -3.3026366164509433 1103 62-63 -3.687139392103223 1103 64-65 -3.6870729539763687 1103 66-67 -3.710115667997865 1103 68-69 -3.66659658775335 1103 70-71 -3.212285030295689 1103 72-73 -3.0298218958607173 1103 74-75 -2.547213315307122 1103 76-77 -2.9839645288157577 1103 78-79 -2.98761200045033 1103 80-81 -2.274282349178069 1103 82-83 -2.277704320671031 1103 84-85 -2.78034987269951 1103 86-87 -3.574260534962324 1103 88-89 -2.6777050151767554 1103 90-91 -3.371719235569934 1103 92-93 -3.558907004182114 1103 94-95 -3.175371713839379 1103 96-97 -3.0557045098520774 1103 98-99 -3.6869723037727127 1103 100-101 -3.986593917556579 1103 102-103 -4.046651465110823 1103 104-105 -4.458726114823545 1103 106-107 -4.770554873602229 1103 108-109 -4.105557951420895 1103 110-111 -3.6427127281880516 1103 112-113 -3.79372824483659 1103 114-115 -3.976769505673051 1103 116-117 -4.060595492047053 1103 118-119 -3.9840579065105395 1103 120-121 -4.199988644011462 1103 122-123 -3.815417601235584 1103 124-125 -3.6249795441561705 1104 1 -0.10644644189988384 1104 2 0.015877352348731222 1104 3 0.14452952438106337 1104 4 0.07645571376656335 1104 5 0.2566159524449887 1104 6 0.014881245740845372 1104 7 -0.5360255523794422 1104 8 -0.28834737264463683 1104 9 -0.3897207739979507 1104 10-11 -0.48365445412664343 1104 12-13 -0.3365353158157447 1104 14-15 0.06832140544565846 1104 16-17 -0.21563112379126892 1104 18-19 -0.24650992785370818 1104 20-21 -0.5685060225598804 1104 22-23 -0.6843241737219827 1104 24-25 -0.2027926596793037 1104 26-27 -0.3416091806107815 1104 28-29 -0.08789048734868743 1104 30-31 -0.5040145880052691 1104 32-33 -0.29956484330016053 1104 34-35 -0.6074943369833008 1104 36-37 -1.9680823788321078 1104 38-39 -1.7424911067116469 1104 40-41 -1.0245785666665697 1104 42-43 -1.7133855758013041 1104 44-45 -1.6796389641277898 1104 46-47 -1.213990953833111 1104 48-49 -0.5702986428675132 1104 50-51 -0.9312476557677236 1104 52-53 -1.331559107284587 1104 54-55 -1.456986272047427 1104 56-57 -1.4440861100778122 1104 58-59 -1.1069995524761467 1104 60-61 -0.7360576690825233 1104 62-63 -1.3962621991207698 1104 64-65 -1.3058448838009333 1104 66-67 -1.6855542644890917 1104 68-69 -2.0854562368761584 1104 70-71 -2.322635907488671 1104 72-73 -2.1157868081414186 1104 74-75 -2.050897525833438 1104 76-77 -1.4848417217982117 1104 78-79 -1.3127874390468186 1104 80-81 -1.6540191912833322 1104 82-83 -1.6492832680394542 1104 84-85 -1.9370165393661765 1104 86-87 -2.252330710400919 1104 88-89 -2.0470032607907918 1104 90-91 -1.688035025043618 1104 92-93 -1.6015385831294857 1104 94-95 -1.1837050471727117 1104 96-97 -1.3119325800275163 1104 98-99 -1.874077566930609 1104 100-101 -1.9459798824688583 1104 102-103 -1.7263005879178408 1104 104-105 -1.532059448156879 1104 106-107 -0.9745022420232843 1104 108-109 -1.2232772496665056 1104 110-111 -0.9894671141529656 1104 112-113 -0.6720615781699237 1104 114-115 -0.5736993302344544 1104 116-117 -0.1616481236259979 1104 118-119 -0.6404614152824699 1104 120-121 -0.2772693457658484 1104 122-123 -0.1255930398320757 1104 124-125 -0.015856737138626897 1105 1 0.442573165943255 1105 2 0.5645048033291218 1105 3 0.06158834791047596 1105 4 0.3892008118057788 1105 5 0.4287728151900936 1105 6 0.3754694810349619 1105 7 0.7665234672283958 1105 8 0.6316526273553649 1105 9 0.5379262848255806 1105 10-11 0.03801221254001774 1105 12-13 0.25934703712542984 1105 14-15 -0.29265898671120283 1105 16-17 0.7861335820910895 1105 18-19 0.2784900721462904 1105 20-21 -0.020466806873606913 1105 22-23 0.3052836694152745 1105 24-25 0.697305379536381 1105 26-27 0.49897905468333903 1105 28-29 0.3044624538277887 1105 30-31 -0.30960282329938593 1105 32-33 -0.04368249035898941 1105 34-35 0.3300546826245423 1105 36-37 0.15740781724633024 1105 38-39 0.31731281485697593 1105 40-41 1.003558688235394 1105 42-43 1.2569085418457533 1105 44-45 0.4971257417545658 1105 46-47 0.36149924224531915 1105 48-49 -0.3574555056126094 1105 50-51 -0.5640907930226255 1105 52-53 -0.6491081268924255 1105 54-55 -0.2562019583219346 1105 56-57 -0.29143905125428304 1105 58-59 0.3307455455630688 1105 60-61 0.02747174268218444 1105 62-63 0.2911887812713907 1105 64-65 0.41199825345396945 1105 66-67 0.6536614217854186 1105 68-69 -0.25212290354282274 1105 70-71 0.041971935648582814 1105 72-73 -0.8765711218669132 1105 74-75 -0.49148576112755293 1105 76-77 -1.3814103492491903 1105 78-79 -1.400924693948781 1105 80-81 -1.2499015442245067 1105 82-83 -0.7548715033335682 1105 84-85 -1.1479969315230392 1105 86-87 -1.6601738476558232 1105 88-89 -1.5538660058888318 1105 90-91 -1.677446789749503 1105 92-93 -0.7880091713647808 1105 94-95 -1.3739011256040854 1105 96-97 -1.3018345408118286 1105 98-99 -1.014371684577668 1105 100-101 -0.8855877256061113 1105 102-103 -0.7053201957609794 1105 104-105 -0.9366672912941318 1105 106-107 -0.7980316537879908 1105 108-109 -0.3648458771174887 1105 110-111 -0.18025142787845638 1105 112-113 -0.029022362483647868 1105 114-115 0.020908512902799714 1105 116-117 0.2315871704916468 1105 118-119 0.6506170160900808 1105 120-121 0.640181634626309 1105 122-123 0.8595049993836135 1105 124-125 1.3452216942339241 1106 1 -0.18977977523321599 1106 2 0.28973700147153636 1106 3 0.41452952438106294 1106 4 0.24136799446831247 1106 5 0.633107180515168 1106 6 0.32593387731979107 1106 7 0.6001147984977493 1106 8 0.5658631536711525 1106 9 0.13080554179152415 1106 10-11 0.018275370434757576 1106 12-13 0.31662257892109125 1106 14-15 0.2986722826386412 1106 16-17 -0.0664205974754708 1106 18-19 0.37059533530418065 1106 20-21 0.16219573182608116 1106 22-23 0.26813196662889993 1106 24-25 0.5223827789171835 1106 26-27 0.23891713517869562 1106 28-29 0.040530565282892894 1106 30-31 -0.1811198511631602 1106 32-33 -0.4337753696159581 1106 34-35 -0.20477503873768654 1106 36-37 -0.5856262384812325 1106 38-39 -0.12889461548357417 1106 40-41 0.4718249421053571 1106 42-43 0.7189828452513254 1106 44-45 0.5129926148195807 1106 46-47 0.5855704496756609 1106 48-49 -0.16047408146400244 1106 50-51 -0.28080905927649624 1106 52-53 -0.05506787921440903 1106 54-55 -0.6950564474860208 1106 56-57 -0.659086110077812 1106 58-59 -1.1394556928270276 1106 60-61 -1.2434260901351522 1106 62-63 -0.9174025499979628 1106 64-65 -1.3449676908184784 1106 66-67 -1.1180104048399713 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6 186.0 7 298.0 8 408.0 9 560.0 10 849.0 11 1086.0 12 1233.0 13 1632.0 14 2158.0 15 1754.0 16 733.0 17 636.0 18 636.0 19 616.0 20 628.0 21 680.0 22 602.0 23 670.0 24 750.0 25 748.0 26 820.0 27 872.0 28 907.0 29 1028.0 30 1175.0 31 1269.0 32 1616.0 33 1862.0 34 2396.0 35 3617.0 36 4209.0 37 7487.0 38 4313.0 39 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.5125288493241 16.536844708209696 16.979887899769206 14.970738542697 2 19.39078139803106 25.670387609671707 36.9464925649792 17.99233842731804 3 16.890124600967972 30.357326742868914 39.36360827927093 13.388940376892183 4 9.179281227473998 21.841210997837504 36.0972093502214 32.8822984244671 5 6.362587081083309 42.11014468856919 36.961540046992866 14.565728183354631 6 21.06683142827721 41.61260426320667 17.17021933889404 20.15034496962208 7 14.781175985995262 41.361342807125936 25.301204819277107 18.556276387601688 8 26.54309545875811 32.44979919678715 21.32633096488518 19.68077437956956 9 23.490886623416742 10.847492534239523 22.11306765523633 43.5485531871074 10-11 22.669138090824838 26.96426732571311 28.45227062094532 21.914323962516733 12-13 26.86232107918855 30.033982082174855 30.774379569560296 12.329317269076306 14-15 21.240860879415095 27.565647204201422 37.39676655339306 13.796725362990422 16-17 12.73401297497683 27.70775409329626 39.65811965811966 19.90011327360725 18-19 11.50344969622078 18.950674492843167 52.957470909278136 16.588404901657913 20-21 12.976006590464422 17.658325610132838 56.59149418185563 12.77417361754711 22-23 12.093502213984141 18.757079600453093 57.50901040057666 11.640407784986099 24-25 11.974050046339203 18.46565750180208 57.42971887550201 12.130573576356708 26-27 12.10894861497271 18.08361651735146 57.54196272268561 12.265472144990218 28-29 12.138811656883947 18.553187107403975 57.45855215734734 11.84944907836474 30-31 12.389043352898774 18.551127587272166 57.29893934713212 11.76088971269694 32-33 12.18412109978375 17.903408505818145 56.7191844300278 13.193285964370302 34-35 12.098651014313665 18.94655545257955 56.847904438265886 12.106889094840902 36-37 12.515703841005047 18.48316342292246 55.76356708886829 13.237565647204203 38-39 12.23766862321079 18.932138811656884 55.36813922356091 13.462053341571414 40-41 12.76902481721759 18.955823293172692 55.0561219235918 13.219029966017917 42-43 13.2510889824836 19.200074143488244 54.02794797598575 13.520888898042404 44-45 13.677229333800192 19.402078205616714 52.539056465814646 14.381635994768441 46-47 13.290148503635352 19.955304731107496 51.274947992832274 15.479598772424874 48-49 14.123147585553482 19.462838429296724 49.14884195133003 17.26517203381976 50-51 13.707842026672159 20.45517738530457 48.94392667730807 16.893053910715206 52-53 14.323817762399077 21.016641950898006 48.26989619377163 16.38964409293129 54-55 13.857307628933926 21.189652331520843 48.0711402207942 16.88189981875103 56-57 14.23510632820143 20.799134957005304 46.96977498584007 17.995983728953195 58-59 13.715939282845548 20.848351286223302 47.70045105348794 17.735258377443206 60-61 14.015178819676857 20.540835555921696 47.42402866881545 18.019956955585993 62-63 14.047986819071156 20.260529296673873 47.21037998146432 18.48110390279065 64-65 13.223421345306258 20.759360711784815 48.19994233224863 17.817275610660296 66-67 14.246140854933218 20.270423347441483 47.75556859958602 17.727867198039277 68-69 14.335437498069181 20.619098126847152 46.77939222935053 18.266072145733144 70-71 13.81568784948562 21.000545789695902 47.16137868536769 18.02238767545079 72-73 14.227165070538565 20.716713005869632 46.37730408814746 18.67881783544434 74-75 14.105798638643174 21.26270479564201 46.18006569936875 18.451430866346065 76-77 14.009885696632685 20.495314591700133 46.560601379878484 18.934198331788693 78-79 14.193182988363711 20.970033982082175 46.06013798784883 18.776645041705283 80-81 14.016064257028113 21.10390279064978 44.90680671403563 19.97322623828648 82-83 13.56296982803007 21.294408402842137 45.465966429821854 19.676655339305942 84-85 14.111831943157243 21.270723921326333 44.096385542168676 20.52105859334775 86-87 14.44547420451035 21.462259293584594 43.43116053959427 20.66110596231078 88-89 13.996498815775924 21.85150859849655 43.36113685511276 20.790855730614766 90-91 14.168760555258064 21.718087078304567 43.07575071054907 21.03740165588829 92-93 14.723509422304604 21.32839048501699 41.766038513026466 22.18206157965194 94-95 14.187150521578845 21.44806351625493 42.081578432483084 22.28320752968314 96-97 14.519616929255482 21.035938626300073 40.90206981773247 23.542374626711975 98-99 14.797652147049737 21.66924106683143 40.19565441252188 23.33745237359695 100-101 14.971681598187622 21.65276490577695 40.16888065080836 23.20667284522706 102-103 14.680410672543227 21.92896641917845 39.32694189004109 24.063681018237236 104-105 14.952116156935432 21.957573885284727 38.73133559880548 24.358974358974358 106-107 14.616414375450521 21.827824116980743 39.418185562763874 24.13757594480486 108-109 15.247657295850066 22.049222531150242 38.09597363814231 24.607146534857378 110-111 14.520646689321387 22.152198537740706 38.52950262588817 24.797652147049735 112-113 14.329557157569518 22.33058702368692 38.48197734294542 24.857878475798145 114-115 14.57998599266675 21.835990606847115 38.4480698718741 25.13595352861204 116-117 14.689434277063143 22.197390238627353 37.77047694573468 25.342698538574826 118-119 14.496395468589082 22.190525231719878 38.48506694129763 24.82801235839341 120-121 15.205346844620202 22.11855330367441 37.81615587411435 24.859943977591037 122-123 14.560377319630094 22.669042077729493 37.41375404197475 25.356826560665663 124-125 13.874671747077905 22.726945059471706 38.05674270119973 25.341640492250654 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 167.0 1 128.0 2 192.5 3 335.0 4 247.0 5 196.5 6 396.5 7 1008.5 8 1484.5 9 1541.0 10 1159.0 11 587.5 12 458.5 13 428.5 14 422.5 15 427.5 16 401.0 17 374.5 18 375.5 19 371.0 20 349.0 21 365.0 22 388.0 23 394.5 24 470.0 25 560.0 26 632.0 27 674.0 28 692.5 29 767.5 30 883.5 31 1107.0 32 1291.5 33 1499.5 34 1926.0 35 2045.0 36 1946.0 37 1832.0 38 1674.0 39 1679.5 40 1544.5 41 1462.5 42 1424.5 43 1312.5 44 1234.0 45 1051.5 46 855.5 47 752.5 48 707.0 49 790.0 50 916.5 51 747.5 52 551.0 53 510.5 54 485.5 55 465.5 56 371.0 57 308.0 58 266.5 59 199.0 60 142.0 61 91.0 62 100.5 63 91.5 64 58.0 65 53.5 66 41.5 67 25.0 68 28.0 69 27.5 70 27.0 71 31.5 72 24.5 73 14.5 74 9.5 75 11.0 76 8.0 77 2.5 78 5.5 79 5.0 80 2.0 81 2.5 82 1.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05560704355885079 2 0.0020595201318092883 3 0.0 4 0.0 5 0.07620224487694367 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0010297600659046442 44-45 0.007208320461332509 46-47 0.008238080527237153 48-49 0.007208320461332509 50-51 0.005148800329523221 52-53 0.006178560395427865 54-55 0.006178560395427865 56-57 0.005148800329523221 58-59 0.004119040263618577 60-61 0.0010297600659046442 62-63 0.0 64-65 0.0020595201318092883 66-67 0.0030892801977139327 68-69 0.0010297600659046442 70-71 0.0030892801977139327 72-73 0.0 74-75 0.0010297600659046442 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0020595201318092883 92-93 0.0 94-95 0.0010297600659046442 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0010297600659046442 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.010297600659046441 114-115 0.018535681186283594 116-117 0.013386880856760375 118-119 0.010297600659046441 120-121 0.006178560395427865 122-123 0.004119040263618577 124-125 0.005148800329523221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 48555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.746164143754505 #Duplication Level Percentage of deduplicated Percentage of total 1 64.90427360559539 22.551745443311706 2 11.91986248592259 8.283389970136957 3 7.272835042380415 7.581093605189991 4 4.895975342303361 6.804654515497889 5 3.4437792661964317 5.982905982905983 6 2.193112441467607 4.57213469261662 7 1.4996147234900126 3.6474101534342496 8 0.9187362930472408 2.553804963443518 9 0.6934977179775947 2.1686746987951806 >10 2.0864204848556693 11.619812583668006 >50 0.08890996384328137 2.078055812995572 >100 0.05927330922885425 5.311502419936155 >500 0.005927330922885425 1.484914015034497 >1k 0.017781992768656274 15.359901143033671 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2814 5.795489650911337 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2402 4.946967356605911 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2242 4.617444135516425 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 721 1.484914015034497 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 496 1.021521985377407 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 473 0.9741530223457935 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.757903408505818 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 360 0.7414272474513438 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 222 0.45721346926166206 No Hit CCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCCTTTTTCCTTTCC 182 0.3748326639892905 No Hit GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.28215425805787253 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 125 0.25744001647616105 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.2306662547626403 No Hit GGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGGAAAAAGGAAAGG 104 0.214190093708166 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.20389249304911955 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 86 0.1771187313355988 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 80 0.16476161054474306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 78 0.1606425702811245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 77 0.1585830501493152 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 66 0.13592832869941301 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 65 0.13386880856760375 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 62 0.12769024817217586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 61 0.1256307280403666 No Hit TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT 60 0.1235712079085573 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 57 0.11739264751312944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC 57 0.11739264751312944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 56 0.11533312738132015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 53 0.10915456698589229 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 52 0.107095046854083 No Hit GTTAAGAGAAGAATGGCCTCTATTAGAAAACTTGAAAGTGATTGGTAAGG 49 0.10091648645865513 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0010297600659046442 0.0 20-21 0.0 0.0 0.0 0.0020595201318092883 0.0 22-23 0.0 0.0 0.0 0.0030892801977139327 0.0 24-25 0.0 0.0 0.0 0.008238080527237153 0.0 26-27 0.0 0.0 0.0 0.008238080527237153 0.0 28-29 0.0 0.0 0.0 0.013386880856760375 0.0 30-31 0.0 0.0 0.0 0.023684481515806814 0.0 32-33 0.0 0.0 0.0 0.054577283492946146 0.0 34-35 0.0 0.0 0.0 0.08341056533827618 0.0 36-37 0.0 0.0 0.0 0.11327360724951085 0.0 38-39 0.0 0.0 0.0 0.18226753166512202 0.0 40-41 0.0 0.0 0.0 0.25229121614663785 0.0 42-43 0.0 0.0 0.0 0.31098753990320255 0.0 44-45 0.0 0.0 0.0 0.39336834517557406 0.0 46-47 0.0 0.0 0.0 0.4706003501184224 0.0 48-49 0.0 0.0 0.0 0.5344454742045104 0.0 50-51 0.0 0.0 0.0 0.6271238801359283 0.0 52-53 0.0 0.0 0.0 0.7053856451446813 0.0 54-55 0.0 0.0 0.0 0.8155699721964782 0.0 56-57 0.0 0.0 0.0 0.9484090206981773 0.0 58-59 0.0 0.0 0.0 1.0802183091339717 0.0 60-61 0.0 0.0 0.0 1.1852538358562454 0.0 62-63 0.0 0.0 0.0 1.3335392853465142 0.0 64-65 0.0 0.0 0.0 1.4663783338482133 0.0 66-67 0.0 0.0 0.0 1.606425702811245 0.0 68-69 0.0 0.0 0.0 1.7557409123674184 0.0 70-71 0.0 0.0 0.0 1.945216764493873 0.0 72-73 0.0 0.0 0.0 2.1202759756976626 0.0 74-75 0.0 0.0 0.0 2.3149006281536404 0.0 76-77 0.0 0.0 0.0 2.535269282257234 0.0 78-79 0.0 0.0 0.0 2.7566676964267325 0.0 80-81 0.0 0.0 0.0 3.009988672639275 0.0 82-83 0.0 0.0 0.0 3.3096488518175264 0.0 84-85 0.0 0.0 0.0 3.572237668623211 0.0 86-87 0.0 0.0 0.0 3.8420348058902274 0.0 88-89 0.0 0.0 0.0 4.133456904541243 0.0 90-91 0.0 0.0 0.0 4.510349088662341 0.0 92-93 0.0 0.0 0.0 4.838842549685923 0.0 94-95 0.0 0.0 0.0 5.184841931829884 0.0 96-97 0.0 0.0 0.0 5.538049634435176 0.0 98-99 0.0 0.0 0.0 5.895376377304088 0.0 100-101 0.0 0.0 0.0 6.288744722479662 0.0 102-103 0.0 0.0 0.0 6.693440428380187 0.0 104-105 0.0 0.0 0.0 7.116671815466996 0.0 106-107 0.0 0.0 0.0 7.5604984038718985 0.0 108-109 0.0 0.0 0.0 8.015652353001752 0.0 110-111 0.0 0.0 0.0 8.475955102461127 0.0 112-113 0.0 0.0 0.0 8.878591288229842 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 685 0.0 87.1192 1 CTGTAAA 30 0.0041629504 59.67665 1 CTTAACC 20 1.7650056E-4 59.492275 32-33 ACCGCTG 15 0.0042270697 59.492275 36-37 ACATTGC 15 0.0042270697 59.492275 48-49 CCGCTGA 15 0.0042270697 59.492275 36-37 ACCTCAG 30 0.004214354 59.492275 8 GCTCTGC 15 0.0042270697 59.492275 26-27 TAACCGC 15 0.0042270697 59.492275 34-35 GATGGAA 15 0.0042270697 59.492275 80-81 AGGCAAC 15 0.0042270697 59.492275 92-93 CCTCAGG 30 0.004214354 59.492275 9 TTAACCG 15 0.0042270697 59.492275 32-33 ACATGCC 20 1.7650056E-4 59.492275 12-13 TAGGCTC 15 0.0042270697 59.492275 84-85 CCTATTC 15 0.0042270697 59.492275 64-65 GGCTCCA 15 0.0042270697 59.492275 86-87 GTTGCTT 15 0.0042270697 59.492275 12-13 CTTACAT 15 0.0042270697 59.492275 44-45 GTATCAA 1065 0.0 56.034416 2 >>END_MODULE