FastQCFastQC Report
Thu 26 May 2016
SRR936198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676507
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30680.1829995341504688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG157950.055.745671
GGTATCA29050.053.861531
TACATGG161850.053.0055852
ACATGGG162900.049.194393
CATGGGA111300.046.557764
ATGGGAT45450.043.065665
ATGGGAG39300.041.781655
CATGGGG49150.038.9763764
GTATCAA42600.038.9640121
CGTATAG1551.9736581E-730.706291
GAGTACA92600.030.5176941
TCAACGC51500.030.1510434
CAACGCA53300.029.244435
ATCAACG53100.029.1304973
ATGGGGG25150.029.0961955
AGTACAT92150.028.7298762
AACGCAG54950.028.4754166
TGGGATA14700.027.9263256
ATGGGAA43900.027.9171945
CATGGGT16250.027.4585114
Produced by FastQC (version 0.11.5)