FastQCFastQC Report
Thu 26 May 2016
SRR936198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676507
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107100.638828230362295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80540.4804036010586296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78610.46889157039010276No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.15216160743736828No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.11201861966576936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.10068553247913668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.083.994461
GTATCAA87600.053.9537472
GTACATG138500.048.162251
TACATGG145900.045.490932
ACATGGG144500.045.1476333
CATGGGA98450.041.476364
ATGGGAT41250.040.9813845
ATCAACG126500.038.7259034
TCAACGC126900.038.6038365
CATGGGG43550.038.270324
CAACGCA128850.038.1388556
AACGCAG132400.037.1611827
TATCAAC133250.036.630173
ATGGGAG33450.032.742665
TATAACG1858.058123E-1032.1761052
AGCGTAC1858.058123E-1032.1751483
GTCGGGA6150.031.9406742
ACGCAGA153300.031.823198
GTGGTAT18050.031.6761631
CGCAGAG154650.031.4299839
Produced by FastQC (version 0.11.5)