FastQCFastQC Report
Thu 26 May 2016
SRR936197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157903
Sequences flagged as poor quality0
Sequence length125
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48653.0810054273826335No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16981.0753437236784609No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13600.861288259247766No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11470.726395318644991No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7270.46040923858318084No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7110.4502764355332071No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5220.33058269950539254No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4250.2691525810149269No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.23242116995877216No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3660.2317878697681488No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3580.22672146824316194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.1830237550901503No Hit
ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.16402474937144956No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT2530.1602249482277094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.1545252465120992No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG2340.14819224460586564No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG2300.1456590438433722No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.14312584308087878No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA2130.13489294060277512No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2120.13425964041215177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.13045983926841162No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2040.1291932388871649No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT1870.11842713564656783No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA1780.1127274339309576No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC1770.11209413374033425No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT1770.11209413374033425No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.10829433259659411No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA208.472335E-489.2383651
TGACCGT208.472335E-489.2383655
TACATAG358.929401E-784.9889142
TGGGATA401.9754007E-674.36536
TACAAGG1250.071.390692
AACATAG359.133941E-567.991134
GGGTATG657.4232958E-964.0685657
GAACAAA5450.063.3128471
CTTACTA1150.062.078869
ATATAGG1200.059.492242
AGGGCGA300.00423198159.492246
AGGCTTA1200.059.492246
GCTTACT1350.052.8819968
TTAAGAC350.00778789250.9933474
GTACTAG350.00778789250.9933471
ACATAGG350.00778789250.9933473
TTTTCGA350.00778789250.9933477
GAGAACA2850.050.098731
TACAAAG1101.7644197E-1048.675472
TCCTAAG505.321973E-447.5937962
Produced by FastQC (version 0.11.5)