##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936197_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 157903 Sequences flagged as poor quality 0 Sequence length 125 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.26622040113234 31.0 20.0 31.0 7.0 34.0 2 25.786850154841897 31.0 23.0 33.0 7.0 34.0 3 26.303287461289525 31.0 25.0 34.0 7.0 34.0 4 28.507102461637842 35.0 23.0 37.0 7.0 37.0 5 30.31647910426021 35.0 31.0 37.0 7.0 37.0 6 29.029847437984078 35.0 27.0 37.0 7.0 37.0 7 29.571515424026142 35.0 29.0 37.0 8.0 37.0 8 28.799725147717268 35.0 26.0 37.0 7.0 37.0 9 29.388909647061805 37.0 22.0 39.0 7.0 39.0 10-11 29.862982337257684 37.0 26.0 39.0 7.0 39.0 12-13 29.96792651184588 37.0 26.5 39.0 4.5 39.0 14-15 30.81260647359455 38.0 27.0 40.0 2.0 41.0 16-17 30.915650747610876 38.0 27.5 40.0 2.0 41.0 18-19 30.7459611280343 38.0 28.0 40.0 2.0 41.0 20-21 30.690769016421473 38.0 28.0 40.0 2.0 41.0 22-23 30.426344021329548 37.5 27.5 40.0 2.0 41.0 24-25 30.26765165956315 37.0 27.0 40.0 2.0 41.0 26-27 30.203561680272067 37.0 27.0 40.0 2.0 41.0 28-29 29.982980057377 37.0 26.5 40.0 2.0 41.0 30-31 29.807669898608637 37.0 25.0 40.0 2.0 41.0 32-33 29.544676161947525 37.0 25.0 40.0 2.0 41.0 34-35 29.407930818287177 37.0 25.0 40.0 2.0 41.0 36-37 29.21335883422101 37.0 25.0 40.0 2.0 41.0 38-39 28.88573681310678 36.0 25.0 40.0 2.0 41.0 40-41 28.898846127052686 36.5 25.0 40.0 2.0 41.0 42-43 28.719590508096744 36.0 24.0 40.0 2.0 41.0 44-45 28.62061202130422 36.0 23.5 40.0 2.0 41.0 46-47 28.565879052329592 36.0 24.0 40.0 2.0 41.0 48-49 28.463525075521048 36.0 23.0 40.0 2.0 41.0 50-51 28.35732063355351 36.0 23.0 40.0 2.0 41.0 52-53 28.237877051100995 35.5 22.5 40.0 2.0 41.0 54-55 28.095023527102082 35.0 22.0 40.0 2.0 41.0 56-57 27.94365528204024 35.0 20.5 40.0 2.0 41.0 58-59 27.743915568418586 35.0 18.5 40.0 2.0 41.0 60-61 27.549134595289516 35.0 14.5 40.0 2.0 41.0 62-63 27.293578969367267 35.0 11.0 39.0 2.0 41.0 64-65 27.052909064425627 34.0 7.5 39.0 2.0 41.0 66-67 26.777071999898673 34.0 4.0 39.0 2.0 40.5 68-69 26.526361120434697 34.0 2.0 38.5 2.0 40.0 70-71 26.21986282717871 34.0 2.0 38.0 2.0 40.0 72-73 25.870927088149053 33.5 2.0 37.0 2.0 40.0 74-75 25.552405590774086 33.0 2.0 37.0 2.0 39.0 76-77 25.26654655073051 33.0 2.0 36.0 2.0 39.0 78-79 24.956853258012828 33.0 2.0 36.0 2.0 39.0 80-81 24.707643300000633 33.0 2.0 35.5 2.0 37.5 82-83 24.417613978201807 32.5 2.0 35.0 2.0 37.0 84-85 24.321608835804263 33.0 2.0 35.0 2.0 37.0 86-87 24.167260913345537 33.0 2.0 35.0 2.0 36.0 88-89 23.990019188995774 33.0 2.0 35.0 2.0 36.0 90-91 23.79999113379733 32.5 2.0 35.0 2.0 36.0 92-93 23.66104823847552 32.0 2.0 35.0 2.0 35.5 94-95 23.52416990177514 32.0 2.0 35.0 2.0 35.0 96-97 23.419802030360408 32.0 2.0 35.0 2.0 35.0 98-99 23.312479813556422 32.0 2.0 35.0 2.0 35.0 100-101 23.19850477824994 32.0 2.0 35.0 2.0 35.0 102-103 22.435352716541168 30.5 2.0 34.0 2.0 35.0 104-105 22.921065464240705 31.5 2.0 35.0 2.0 35.0 106-107 23.00079479173923 32.0 2.0 35.0 2.0 35.0 108-109 22.947024439054356 32.0 2.0 35.0 2.0 35.0 110-111 22.827441530559902 32.0 2.0 35.0 2.0 35.0 112-113 22.799883472764925 32.0 2.0 35.0 2.0 35.0 114-115 22.675357656282657 32.0 2.0 35.0 2.0 35.0 116-117 22.5446508299399 32.0 2.0 35.0 2.0 35.0 118-119 22.363859458021697 32.0 2.0 35.0 2.0 35.0 120-121 22.164518723520136 32.0 2.0 35.0 2.0 35.0 122-123 21.911749618436637 31.0 2.0 35.0 2.0 35.0 124-125 20.406376066319197 28.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5462113313146091 1101 2 -0.4057341857354331 1101 3 -0.06275972238773875 1101 4 -0.23648899632431863 1101 5 -0.25836507621885474 1101 6 0.0016973167431473257 1101 7 -0.9385746310016891 1101 8 -0.24819800228445743 1101 9 -0.33446974166954035 1101 10-11 -0.32198463438074754 1101 12-13 -0.17441431528887108 1101 14-15 -0.03498127519756977 1101 16-17 -0.3519687347987812 1101 18-19 -0.6042046369973484 1101 20-21 -0.8675896451059373 1101 22-23 -0.6813596811598259 1101 24-25 -0.48469601051334976 1101 26-27 -0.39590025159736797 1101 28-29 -0.4069841595593324 1101 30-31 -0.5701352135951154 1101 32-33 -0.6150054763087844 1101 34-35 -0.605992052466803 1101 36-37 -0.7264730341060854 1101 38-39 -0.3435344566856422 1101 40-41 -0.2168073936205701 1101 42-43 0.012324563940648403 1101 44-45 -0.005857130045807679 1101 46-47 -0.3663073553981029 1101 48-49 -0.49789213833332724 1101 50-51 -0.2595150162453592 1101 52-53 -0.3398045305172097 1101 54-55 -0.29971035046806094 1101 56-57 -0.38505413255539267 1101 58-59 -0.30394243501883267 1101 60-61 -0.1496445430425588 1101 62-63 -0.1909747524942631 1101 64-65 0.19512253915185696 1101 66-67 0.08951122668504397 1101 68-69 0.43232397189655813 1101 70-71 -0.07301840109134972 1101 72-73 -0.07634181755194192 1101 74-75 -0.2891176822457169 1101 76-77 -0.29967613310840235 1101 78-79 -0.08801077963552828 1101 80-81 -0.026065678975978557 1101 82-83 -0.1495147635835714 1101 84-85 -0.36389780744507405 1101 86-87 -0.04134842107837855 1101 88-89 0.013252946632686502 1101 90-91 0.10986804335385969 1101 92-93 0.06763505896928734 1101 94-95 0.3209333497252871 1101 96-97 0.24802282736171932 1101 98-99 0.07354792323181059 1101 100-101 0.15942202222665713 1101 102-103 0.367894774581476 1101 104-105 0.5588735106727896 1101 106-107 0.11188193221341791 1101 108-109 0.14161423893190772 1101 110-111 0.16572464159368394 1101 112-113 0.18664918866864255 1101 114-115 -0.0024796464618326297 1101 116-117 0.13053214284262538 1101 118-119 0.2583246978944942 1101 120-121 0.3815085756032026 1101 122-123 0.3982666845426621 1101 124-125 -0.07973054651350253 1102 1 1.055802068678588 1102 2 0.8683704974601589 1102 3 1.2159873442381155 1102 4 1.26861252392872 1102 5 0.8730330804318278 1102 6 1.1289122267863405 1102 7 0.9929629672025762 1102 8 1.1734256415614084 1102 9 0.7845147156987586 1102 10-11 1.151096343750737 1102 12-13 0.8221676661415955 1102 14-15 1.3462298257104983 1102 16-17 1.2791403357042306 1102 18-19 0.5307220711616836 1102 20-21 0.641057431722956 1102 22-23 0.8709053938325191 1102 24-25 0.9150676190482621 1102 26-27 1.2114102269252314 1102 28-29 0.7424138610508066 1102 30-31 0.7227175565303803 1102 32-33 1.0433457123454346 1102 34-35 1.207088245461982 1102 36-37 1.51801732402064 1102 38-39 1.2277082736479983 1102 40-41 1.5350121486030126 1102 42-43 1.3873458202750335 1102 44-45 1.3513598206054844 1102 46-47 0.801209564644072 1102 48-49 0.6622870950782307 1102 50-51 0.574915143942377 1102 52-53 0.7424914936980045 1102 54-55 0.6428380289490043 1102 56-57 0.618049292265205 1102 58-59 0.7348121138367247 1102 60-61 0.5497228684460644 1102 62-63 0.624962396776219 1102 64-65 0.7839485092909548 1102 66-67 0.9741063360276279 1102 68-69 0.7733180881431991 1102 70-71 0.8576519386698997 1102 72-73 0.6234932332932104 1102 74-75 0.5899167700210626 1102 76-77 1.2183152283173015 1102 78-79 1.1279195676079503 1102 80-81 1.0176373445250242 1102 82-83 0.9720544640462627 1102 84-85 0.732248844257132 1102 86-87 0.8936242007629325 1102 88-89 0.9014446277536585 1102 90-91 0.667268648734261 1102 92-93 0.7554220194835253 1102 94-95 0.8311959079676434 1102 96-97 0.713630078322506 1102 98-99 0.6637342987717254 1102 100-101 0.8402624126630087 1102 102-103 0.8619089908179163 1102 104-105 0.7887395107408111 1102 106-107 0.9605538364408766 1102 108-109 1.1241670396665242 1102 110-111 1.2617692628908301 1102 112-113 1.097381086774277 1102 114-115 1.0372952064443375 1102 116-117 0.8348565315104501 1102 118-119 0.7405288947451538 1102 120-121 0.9689146226052117 1102 122-123 0.7952329804988558 1102 124-125 0.3234766092189041 1103 1 1.7956764459798684 1103 2 1.861622859133572 1103 3 1.0897815162779025 1103 4 1.3817229352847455 1103 5 1.1329254514353089 1103 6 1.0971066920568298 1103 7 1.2793127447804231 1103 8 1.0403555494269874 1103 9 1.141506435263576 1103 10-11 1.2156404199907165 1103 12-13 1.4767885488326584 1103 14-15 1.5507332495121666 1103 16-17 1.4549038184852527 1103 18-19 1.0352121662950502 1103 20-21 1.0636095384855935 1103 22-23 1.7376770237620462 1103 24-25 1.866367997249224 1103 26-27 1.9639468391109638 1103 28-29 2.3015931483002703 1103 30-31 2.6578652548359756 1103 32-33 2.61129692941946 1103 34-35 2.2822369434176935 1103 36-37 2.277801399594189 1103 38-39 2.2761413220810454 1103 40-41 2.210159180475191 1103 42-43 2.195121432144216 1103 44-45 2.1237728093927437 1103 46-47 2.6986038099915355 1103 48-49 2.283961175816639 1103 50-51 1.919969374961525 1103 52-53 2.0791819853592557 1103 54-55 2.237521556381072 1103 56-57 2.29215502192649 1103 58-59 2.352279333643125 1103 60-61 2.14886316934075 1103 62-63 1.9258820748888823 1103 64-65 2.063234092670104 1103 66-67 2.157458829321641 1103 68-69 1.7291168918952167 1103 70-71 2.075825577896172 1103 72-73 1.742684849560849 1103 74-75 1.9032987529936847 1103 76-77 2.1430748957670254 1103 78-79 2.0672741268455503 1103 80-81 1.9581415014034498 1103 82-83 1.9646820443931396 1103 84-85 1.9875928072034341 1103 86-87 2.1468772288697586 1103 88-89 1.888365871984842 1103 90-91 1.7727900495773028 1103 92-93 1.956951484170883 1103 94-95 1.7580548406745287 1103 96-97 1.904754851318625 1103 98-99 1.8228452765961514 1103 100-101 1.767604509595749 1103 102-103 1.3910821135582871 1103 104-105 1.4770402721409823 1103 106-107 1.5355109347897873 1103 108-109 1.6914185016548302 1103 110-111 1.810235632994626 1103 112-113 1.8406542744919108 1103 114-115 1.731635520667691 1103 116-117 1.8805488725244253 1103 118-119 1.719369633819813 1103 120-121 1.5118662562001184 1103 122-123 1.6606717435984422 1103 124-125 1.4522398754090737 1104 1 1.3622756362632664 1104 2 1.2401653692550312 1104 3 1.1189313138487513 1104 4 1.2724111943940564 1104 5 1.144463912973773 1104 6 1.5649204572390154 1104 7 1.2635232710962114 1104 8 1.1409628368763798 1104 9 1.401830321903251 1104 10-11 1.0866930515696644 1104 12-13 0.6334687107759756 1104 14-15 0.9493162454635886 1104 16-17 1.0113815512787774 1104 18-19 1.136831599493437 1104 20-21 1.106726947392474 1104 22-23 1.0555920035191306 1104 24-25 1.336712126803878 1104 26-27 1.6055460293943682 1104 28-29 1.458273310243591 1104 30-31 1.5661648499776746 1104 32-33 1.6316410589741146 1104 34-35 1.5475203442274115 1104 36-37 1.576991682994997 1104 38-39 1.3049874759271987 1104 40-41 1.275341366710009 1104 42-43 1.280951391658384 1104 44-45 0.9767080320648063 1104 46-47 0.638381137926757 1104 48-49 0.9391231191364753 1104 50-51 1.026244678605245 1104 52-53 0.8773601230110799 1104 54-55 1.0356996940328962 1104 56-57 0.8863857911572595 1104 58-59 1.1441821676512234 1104 60-61 1.0254826025391282 1104 62-63 0.8721371356176277 1104 64-65 1.0298332829535077 1104 66-67 1.0903535661637491 1104 68-69 0.6938942198304403 1104 70-71 0.8029510839690452 1104 72-73 1.047037076281498 1104 74-75 0.9470234493499632 1104 76-77 1.0126093087224923 1104 78-79 0.8080636005297634 1104 80-81 0.6456921086908984 1104 82-83 0.9379613966198619 1104 84-85 0.8382000946528265 1104 86-87 0.7723833017442523 1104 88-89 0.947677612875534 1104 90-91 0.9062920738688014 1104 92-93 0.7652510793125842 1104 94-95 0.8608888487716939 1104 96-97 0.8367386569866433 1104 98-99 0.8715294871224692 1104 100-101 0.897867667490484 1104 102-103 0.969118550805252 1104 104-105 0.9220807579709458 1104 106-107 1.1026161979476825 1104 108-109 1.1077140482135341 1104 110-111 1.2141830014156767 1104 112-113 0.9906542744919093 1104 114-115 1.0647326866595925 1104 116-117 1.5275124352774618 1104 118-119 1.4608676095283144 1104 120-121 1.465408766321577 1104 122-123 1.1072304480518795 1104 124-125 1.2361467580001637 1105 1 -0.1788301329675015 1105 2 0.17045383079349108 1105 3 0.03996496769490676 1105 4 -0.08666332483671368 1105 5 -0.07470676010315103 1105 6 -0.3467982927609832 1105 7 -0.5241570173653258 1105 8 -0.23896504773900418 1105 9 -0.44859035117367085 1105 10-11 -0.10158819843033484 1105 12-13 -0.14952407768556242 1105 14-15 0.013378745463587194 1105 16-17 -0.24832397756737734 1105 18-19 -0.24084267935272052 1105 20-21 -0.5183031006844487 1105 22-23 -0.4125870830193321 1105 24-25 -0.08041527704227391 1105 26-27 -0.13976647060563252 1105 28-29 0.31275047370512965 1105 30-31 -0.014333948099249483 1105 32-33 0.06355211666642191 1105 34-35 -0.09388590577258782 1105 36-37 0.11632461568730434 1105 38-39 0.13641776438873876 1105 40-41 0.16284136671000837 1105 42-43 0.22842735319684593 1105 44-45 0.2314556282186544 1105 46-47 0.41452296484983364 1105 48-49 0.3377709556749373 1105 50-51 0.12224828437447854 1105 52-53 0.37883247878031057 1105 54-55 0.31767084787904665 1105 56-57 0.28314059884956677 1105 58-59 0.19739731188199272 1105 60-61 0.10327707369297556 1105 62-63 0.3258931452330103 1105 64-65 0.32475515795350773 1105 66-67 0.6984665469329769 1105 68-69 0.21967546983044173 1105 70-71 0.4060460358921212 1105 72-73 0.3214661628199593 1105 74-75 0.2830510935807311 1105 76-77 0.3046465683378763 1105 78-79 0.21509485052976274 1105 80-81 0.4004096567678239 1105 82-83 0.3351669254660159 1105 84-85 0.37278543119128926 1105 86-87 0.1804361863596391 1105 88-89 -0.02140291597062216 1105 90-91 0.13048077579187733 1105 92-93 0.25650708892796814 1105 94-95 -0.027061872382152075 1105 96-97 -0.07035268916720483 1105 98-99 -0.1615534455698402 1105 100-101 -0.36511310174028466 1105 102-103 -0.27805692996397724 1105 104-105 -0.4118134727982863 1105 106-107 -0.30537659051385546 1105 108-109 -0.26211768255569723 1105 110-111 -0.5657448831997058 1105 112-113 -0.5426089466619359 1105 114-115 -0.6223466402634834 1105 116-117 -0.6209851608763834 1105 118-119 -0.20472734239476154 1105 120-121 -0.046790752909192435 1105 122-123 0.07396722689803426 1105 124-125 0.07148329646170382 1106 1 0.6293193768364205 1106 2 0.6487626543229013 1106 3 0.6257186441654952 1106 4 0.9855333663397552 1106 5 0.5851124800929277 1106 6 0.5619792807684298 1106 7 0.3897676149876155 1106 8 0.0455631385355062 1106 9 0.2336553596106441 1106 10-11 0.31316363980495865 1106 12-13 0.39304638800071245 1106 14-15 0.48559075526750917 1106 16-17 0.8526334366483113 1106 18-19 0.2025932887845343 1106 20-21 0.0512970831390831 1106 22-23 0.3367006008041997 1106 24-25 -2.806839909395215 1106 26-27 0.06289897057083849 1106 28-29 0.5771270055678741 1106 30-31 0.39112714258702397 1106 32-33 -3.967039182353183 1106 34-35 -0.08434240087062506 1106 36-37 0.374880069118678 1106 38-39 0.6782529359573672 1106 40-41 0.5106354843570671 1106 42-43 0.26601926496155315 1106 44-45 0.4704108978264969 1106 46-47 0.5854022540655173 1106 48-49 0.37468118851807475 1106 50-51 0.8305433211391815 1106 52-53 1.2110705302509004 1106 54-55 0.5885654557221862 1106 56-57 0.5573133929672132 1106 58-59 0.7683148976663077 1106 60-61 0.9927148800655239 1106 62-63 0.9229289908212444 1106 64-65 1.1210097535417418 1106 66-67 0.773573777325133 1106 68-69 0.8703648080657373 1106 70-71 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122-123 -2.2923098522852356 2216 124-125 -2.0127423011478562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13416.0 3 2893.0 4 3899.0 5 4224.0 6 4249.0 7 2282.0 8 1381.0 9 1238.0 10 1324.0 11 1347.0 12 1300.0 13 1296.0 14 1314.0 15 1153.0 16 992.0 17 865.0 18 755.0 19 707.0 20 698.0 21 663.0 22 904.0 23 1629.0 24 2511.0 25 2248.0 26 3459.0 27 2345.0 28 2520.0 29 2697.0 30 3021.0 31 3733.0 32 4835.0 33 5988.0 34 8083.0 35 11480.0 36 17882.0 37 28117.0 38 10442.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.01112075134735 25.090086952116174 9.071391930488971 17.82740036604751 2 15.348030119757066 33.630140022672144 32.01395793620134 19.00787192136945 3 13.488027459896266 44.56723431473753 24.560647992755047 17.38409023261116 4 8.222136374863048 27.563757496691004 30.884783696319897 33.32932243212605 5 7.664198906923871 54.788066091207895 28.07736395128655 9.470371050581686 6 20.71588253548064 50.201072810522916 15.800206455862142 13.282838198134298 7 21.880026852098137 47.95949386328229 17.799647882864058 12.360831401755519 8 22.42607709894174 45.71092013350137 16.716803566791853 15.146199200765038 9 23.20567950804618 29.362701946156132 15.015737709070873 32.415880836726814 10-11 17.88425062459825 42.23297435426516 23.117259810704773 16.76551521043182 12-13 19.79373151531656 42.0614815612314 22.13222208852382 16.012564834928213 14-15 19.06934768841039 42.42178594046865 20.18999366687777 18.31887270424319 16-17 17.522854727215726 42.931421568782675 22.300577265919145 17.24514643808245 18-19 17.103917365159486 41.766756702839466 24.001975940392466 17.127349991608586 20-21 19.42139862442843 40.484046663035635 23.94932171401791 16.14523299851803 22-23 19.39562407487821 40.35385215167084 23.395953711422223 16.854570062028724 24-25 18.53756467660116 40.55895782800615 24.041646348028195 16.861831147364487 26-27 17.806987267378712 40.86788282568563 24.02067111458726 17.304458792348395 28-29 17.732573788089407 39.33591511163605 25.373096519032433 17.558414581242104 30-31 17.862344998796722 41.00138060315892 24.24035161049258 16.895922787551772 32-33 17.241499420522985 41.43545639356305 24.376033084021 16.947011101892958 34-35 17.4092158427379 40.9117680586693 24.796709351369834 16.882306747222962 36-37 17.323031617612134 40.94742008517915 24.355219201722583 17.37432909548614 38-39 16.980415129589463 41.74981396747993 24.419182723516094 16.850588179414512 40-41 16.646400932223784 41.59352003141189 25.230522729287785 16.52955630707654 42-43 16.776914722152732 41.81657552334841 23.92694179599307 17.479567958505775 44-45 16.70498157084774 42.51339438385834 23.6621448746691 17.119479170624817 46-47 16.708834304930605 41.896055452291456 24.19863015867487 17.196480084103065 48-49 16.615897101385027 42.66416724191434 23.911515297366105 16.808420359334526 50-51 16.761662296867677 41.94405390685362 23.894884168661576 17.39939962761713 52-53 16.537208322910168 42.0714812715522 23.845878601533236 17.545431804004394 54-55 16.876397236242962 42.59662700046232 23.443486741692578 17.083489021602144 56-57 17.012504631051637 42.32575371354927 23.126759403805536 17.534982251593554 58-59 16.76034261648802 41.88850714839853 23.83052833235699 17.520621902756446 60-61 17.518199637115544 42.62055775277626 23.12580944449546 16.73543316561274 62-63 16.817499572516954 42.86451637418382 23.686360441035838 16.63162361226338 64-65 17.208829555134056 41.848557486787655 23.916175590478876 17.02643736759942 66-67 17.41037219052203 42.35290019822296 23.230400942350684 17.00632666890433 68-69 17.119370752930596 42.81425938709207 23.521719030037428 16.5446508299399 70-71 17.730822086977447 42.01693444709727 23.860217981925615 16.39202548399967 72-73 17.581363241990335 42.63820193409878 22.77379150491124 17.00664331899964 74-75 17.22961954370577 42.50945995155238 23.37581735564668 16.885103149095173 76-77 17.187133873327294 41.75126501713077 24.42955485329601 16.63204625624592 78-79 16.909431739738952 42.25283876810447 23.81842016934447 17.019309322812106 80-81 17.21594903200066 42.48082683672888 23.283281508267734 17.01994262300273 82-83 18.083253643059347 41.044502004395106 23.46345541250008 17.408788940045472 84-85 17.117470852358725 41.00840389352957 23.625263611204346 18.248861642907354 86-87 17.84038301995529 40.990354838096806 23.764906303236796 17.40435583871111 88-89 18.200155159038015 40.11684425515745 24.080682699767262 17.60231788603727 90-91 18.205480579849652 40.23451106058783 24.08028979816723 17.479718561395288 92-93 17.948044052361258 40.837729492156576 23.644262616923047 17.569963838559115 94-95 17.25901344496305 40.0790358637898 24.430504803581947 18.231445887665213 96-97 17.845132771384964 40.77788262414267 23.886816589931794 17.490168014540572 98-99 17.315693812023838 41.359885499325536 23.725325041322836 17.59909564732779 100-101 16.560483334705484 41.788313078282236 23.610697706820012 18.04050588019227 102-103 16.652417322136515 42.366784461248116 23.528517688186344 17.452280528429025 104-105 15.926233193796191 43.4548425299076 23.315263167894212 17.303661108401993 106-107 15.79229020347935 43.606517925561896 23.6429960165418 16.958195854416953 108-109 16.504857443224278 43.96461096122912 22.391103342579573 17.13942825296703 110-111 15.819888855464606 44.80771362074698 22.031000142492992 17.34139738129542 112-113 15.716294180604548 44.399726414317655 23.110073906132243 16.773905498945556 114-115 16.076692895932617 44.239958202055064 22.57943984420766 17.10390905780466 116-117 15.72421043298734 44.427908272800394 22.634782113069416 17.213099181142855 118-119 15.972881914098618 43.246127344808805 22.80560094235665 17.975389798735925 120-121 16.027561224295926 43.8044242351317 22.704761784133296 17.46325275643908 122-123 16.13870540775033 43.58815222003381 22.54453683590559 17.728605536310265 124-125 16.406591388384008 43.63849958518837 22.113576056186393 17.841332970241226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3958.0 1 4362.5 2 4622.5 3 3537.0 4 2038.0 5 1232.0 6 890.5 7 787.0 8 767.0 9 739.0 10 609.5 11 451.5 12 375.5 13 349.5 14 326.5 15 307.0 16 317.5 17 344.5 18 377.5 19 430.5 20 500.0 21 557.0 22 644.0 23 750.0 24 832.5 25 975.0 26 1194.5 27 1322.5 28 1482.0 29 1838.0 30 2533.0 31 3112.5 32 3887.5 33 4843.5 34 5466.5 35 6160.0 36 7072.0 37 7748.5 38 7118.0 39 6503.5 40 6272.5 41 5697.0 42 4873.5 43 4517.0 44 4694.0 45 4639.0 46 3972.5 47 3115.0 48 3042.5 49 3023.0 50 3196.5 51 2989.5 52 2305.5 53 1951.0 54 1694.0 55 1486.5 56 1323.5 57 1202.5 58 1103.5 59 1011.0 60 880.5 61 793.0 62 765.5 63 685.0 64 549.5 65 488.0 66 411.5 67 336.5 68 277.5 69 192.5 70 167.5 71 150.0 72 127.5 73 137.5 74 121.5 75 78.5 76 55.5 77 48.0 78 42.5 79 26.5 80 14.0 81 8.0 82 6.5 83 9.5 84 10.0 85 6.5 86 3.5 87 3.5 88 1.0 89 0.0 90 2.5 91 3.0 92 1.5 93 1.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 6.333001906233573E-4 8 0.0012666003812467146 9 0.0012666003812467146 10-11 9.499502859350361E-4 12-13 0.0012666003812467146 14-15 0.0018999005718700723 16-17 0.0022165506671817507 18-19 0.0022165506671817507 20-21 0.003166500953116787 22-23 0.09721157926068535 24-25 0.0012666003812467146 26-27 9.499502859350361E-4 28-29 9.499502859350361E-4 30-31 6.333001906233573E-4 32-33 0.0012666003812467146 34-35 6.333001906233573E-4 36-37 3.1665009531167866E-4 38-39 3.1665009531167866E-4 40-41 6.333001906233573E-4 42-43 9.499502859350361E-4 44-45 6.333001906233573E-4 46-47 9.499502859350361E-4 48-49 0.0 50-51 6.333001906233573E-4 52-53 9.499502859350361E-4 54-55 0.0012666003812467146 56-57 9.499502859350361E-4 58-59 3.1665009531167866E-4 60-61 9.499502859350361E-4 62-63 0.0012666003812467146 64-65 9.499502859350361E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.1665009531167866E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 3.1665009531167866E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 6.333001906233573E-4 104-105 0.0 106-107 0.0 108-109 6.333001906233573E-4 110-111 3.1665009531167866E-4 112-113 0.0 114-115 3.1665009531167866E-4 116-117 0.0 118-119 6.333001906233573E-4 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 157903.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.72381145386725 #Duplication Level Percentage of deduplicated Percentage of total 1 72.06835897082155 26.46624826634073 2 8.82251500310409 6.479927550458192 3 5.297647789197765 5.836494556784862 4 3.597295992274264 5.284256790561294 5 2.455680485617714 4.509097357238304 6 1.695178312754363 3.735204524296562 7 1.0829826860729805 2.783987637980279 8 0.8036145409395047 2.3609431106438765 9 0.596675174173967 1.9720967936011347 >10 3.228254121542388 21.66393292084381 >50 0.24487825067255298 6.048016820453063 >100 0.09484720976753812 5.874492568222263 >500 0.005173484169138443 1.2412683736217804 >1k 0.006897978892184589 5.744032728953852 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4865 3.0810054273826335 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1698 1.0753437236784609 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1360 0.861288259247766 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1147 0.726395318644991 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 727 0.46040923858318084 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 711 0.4502764355332071 No Hit ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 522 0.33058269950539254 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 425 0.2691525810149269 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 367 0.23242116995877216 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 366 0.2317878697681488 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 358 0.22672146824316194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 289 0.1830237550901503 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 259 0.16402474937144956 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 253 0.1602249482277094 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 244 0.1545252465120992 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 234 0.14819224460586564 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 230 0.1456590438433722 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 0.14312584308087878 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 213 0.13489294060277512 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 212 0.13425964041215177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 206 0.13045983926841162 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 204 0.1291932388871649 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 187 0.11842713564656783 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 178 0.1127274339309576 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 177 0.11209413374033425 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 177 0.11209413374033425 No Hit AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 171 0.10829433259659411 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.333001906233574E-4 0.0 16-17 0.0 0.0 0.0 6.333001906233574E-4 0.0 18-19 0.0 0.0 0.0 6.333001906233574E-4 0.0 20-21 0.0 0.0 0.0 0.0012666003812467149 0.0 22-23 0.0 0.0 0.0 0.0012666003812467149 0.0 24-25 0.0 0.0 0.0 0.0018999005718700723 0.0 26-27 0.0 0.0 0.0 0.0028498508578051083 0.0 28-29 0.0 0.0 0.0 0.006333001906233574 0.0 30-31 0.0 0.0 0.0 0.024382057338999258 0.0 32-33 0.0 0.0 0.0 0.05383051620298537 0.0 34-35 0.0 0.0 0.0 0.08327897506697149 0.0 36-37 0.0 0.0 0.0 0.11209413374033425 0.0 38-39 0.0 0.0 0.0 0.15800839756052765 0.0 40-41 0.0 0.0 0.0 0.2080391126197729 0.0 42-43 0.0 0.0 0.0 0.25616992710714803 0.0 44-45 0.0 0.0 0.0 0.3207665465507305 0.0 46-47 0.0 0.0 0.0 0.4018289709505203 0.0 48-49 0.0 0.0 0.0 0.47624174334876473 0.0 50-51 0.0 0.0 0.0 0.5465380645079574 0.0 52-53 0.0 0.0 0.0 0.6181009860483968 0.0 54-55 0.0 0.0 0.0 0.7045464620684851 0.0 56-57 0.0 0.0 0.0 0.7985915403760537 0.0 58-59 0.0 0.0 0.0 0.9227183777382317 0.0 60-61 0.0 0.0 0.0 1.0351291615738776 0.0 62-63 0.0 0.0 0.0 1.1541895974110687 0.0 64-65 0.0 0.0 0.0 1.2786330848685585 0.0 66-67 0.0 0.0 0.0 1.4255587290931775 0.0 68-69 0.0 0.0 0.0 1.5978163809427306 0.0 70-71 0.0 0.0 0.0 1.7716572832688424 0.0 72-73 0.0 0.0 0.0 1.9416983844512137 0.0 74-75 0.0 0.0 0.0 2.134538292496026 0.0 76-77 0.0 0.0 0.0 2.3634763114063695 0.0 78-79 0.0 0.0 0.0 2.639278544422842 0.0 80-81 0.0 0.0 0.0 2.894181871148743 0.0 82-83 0.0 0.0 0.0 3.153518299209008 0.0 84-85 0.0 0.0 0.0 3.4046218247911693 0.0 86-87 0.0 0.0 0.0 3.703539514765394 0.0 88-89 0.0 0.0 0.0 4.061037472372279 0.0 90-91 0.0 0.0 0.0 4.4270849825525795 0.0 92-93 0.0 0.0 0.0 4.771600286251687 0.0 94-95 0.0 0.0 0.0 5.179128958917817 0.0 96-97 0.0 0.0 0.0 5.670569906841542 0.0 98-99 0.0 0.0 0.0 6.119579741993502 0.0 100-101 0.0 0.0 0.0 6.615453791251591 0.0 102-103 0.0 0.0 0.0 7.147425951375212 0.0 104-105 0.0 0.0 0.0 7.623351044628665 0.0 106-107 0.0 0.0 0.0 8.126824696174234 0.0 108-109 0.0 0.0 0.0 8.660696756869722 0.0 110-111 0.0 0.0 0.0 9.26359853834316 0.0 112-113 0.0 0.0 0.0 9.888032526297792 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGA 20 8.472335E-4 89.238365 1 TGACCGT 20 8.472335E-4 89.238365 5 TACATAG 35 8.929401E-7 84.988914 2 TGGGATA 40 1.9754007E-6 74.3653 6 TACAAGG 125 0.0 71.39069 2 AACATAG 35 9.133941E-5 67.99113 4 GGGTATG 65 7.4232958E-9 64.068565 7 GAACAAA 545 0.0 63.312847 1 CTTACTA 115 0.0 62.07886 9 ATATAGG 120 0.0 59.49224 2 AGGGCGA 30 0.004231981 59.49224 6 AGGCTTA 120 0.0 59.49224 6 GCTTACT 135 0.0 52.881996 8 TTAAGAC 35 0.007787892 50.993347 4 GTACTAG 35 0.007787892 50.993347 1 ACATAGG 35 0.007787892 50.993347 3 TTTTCGA 35 0.007787892 50.993347 7 GAGAACA 285 0.0 50.09873 1 TACAAAG 110 1.7644197E-10 48.67547 2 TCCTAAG 50 5.321973E-4 47.593796 2 >>END_MODULE