FastQCFastQC Report
Thu 26 May 2016
SRR936197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157903
Sequences flagged as poor quality0
Sequence length125
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60183.8112005471713646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57483.640209495703058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49303.122169939773152No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16891.0696440219628507No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.8302565499072215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.8207570470478712No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9040.5725033723235151No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.552871066414191No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.4756084431581414No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4500.28498508578051085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3660.2317878697681488No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3290.20835576271508457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2870.18175715470890358No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG2470.15642514708396926No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2240.14185924269963204No Hit
GTTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.1380594415558919No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2080.13172643964965833No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA1970.1247601375528014No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.11969373602781454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1850.11716053526532111No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG1650.10449453145285398No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT1630.10322793107160726No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1620.1025946308809839No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA1620.1025946308809839No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT1620.1025946308809839No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1590.10069473030911381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC401.974433E-674.371448
GGTATCA16100.072.870091
AGGTAAG250.002048785871.4418262
ACTATAC508.84429E-871.4418263
GTGTATC359.087321E-568.061391
TGTATCA401.762168E-459.5348552
ACGCAAC150.004234514759.49714780-81
GGGTATG603.13672E-759.4971477
ATCGTCG150.004234514759.49714756-57
ACGCTGC150.004234514759.49714784-85
CGCAACG150.004234514759.49714780-81
GTGCCCG150.004234514759.49714768-69
ATGGGAG1500.055.5658655
GTATGGT551.29854925E-554.139751
ATGGGTA901.6079866E-952.919875
ACCTATT453.1632942E-452.8863568
GGTAAGG350.007765870551.0298733
TAACGCA350.007765870551.0298734
CCACGCA350.007765870551.0298734
GTTAGAG350.00778535250.9975558
Produced by FastQC (version 0.11.5)