FastQCFastQC Report
Thu 26 May 2016
SRR936196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679287
Sequences flagged as poor quality0
Sequence length125
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33160.48815890779596843No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG17620.259389624709438No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12920.1901994296961373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18100.060.4774741
GTACATG96700.052.231871
CAGCGTA350.007797061550.9964039
TACATGG97850.048.6388782
ATGGGAG25500.046.430065
ATGGGCG1301.8189894E-1145.7660035
CGCAGAA2900.045.1314242
ACATGGG99900.044.1305243
GTCGGGA5700.043.835792
GTACATA6600.042.3651051
TACATGA7350.042.089172
CGGGAGT5850.041.697914
GTATCAA28250.041.6966481
CATGGGA80300.041.4173774
TCGGGAG6250.039.981183
ATGGGAT28350.039.663875
CATGGGG19350.039.6638684
TGGGCGT758.1885155E-539.6638686
GAGAACA13350.036.541551
GTACTTG4150.035.8382071
Produced by FastQC (version 0.11.5)