FastQCFastQC Report
Thu 26 May 2016
SRR936196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679287
Sequences flagged as poor quality0
Sequence length125
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109891.6177256446833224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90371.3303655156068053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86111.2676527005521967No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26010.3829014834672237No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.3263716220095482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.27867455140463454No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG17230.2536483106551428No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16800.2473181438773302No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13360.19667680965482925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8890.1308725178017539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8580.12630890919449364No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT7080.10422693206258915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44650.091.673241
TATAACG701.3340468E-859.5370522
GTATCAA77100.053.2049642
GTACATG82600.044.2889751
TACATGG84150.043.0166662
ACATGGG83400.042.33273
ATGGGCG1700.042.0261575
ATGGGAT22700.041.4398885
CATGGGA65950.040.5339474
CGGGAGT6100.040.016714
ATGGGAG20000.039.8898245
ATCAACG112950.037.5302164
TCAACGC113050.037.4443555
AACGCGG801.1925765E-437.210665
CAACGCA114550.037.137316
GTGGTAT10800.036.962251
GTCGGGA6800.036.7728842
CAACGCG650.001932426836.6381844
AACGCAG116050.036.6060267
TGGTATC10950.036.429062
Produced by FastQC (version 0.11.5)