##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936196_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679287 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.500367296886296 31.0 31.0 34.0 30.0 34.0 2 31.29704675637838 31.0 31.0 34.0 28.0 34.0 3 31.56742731717227 31.0 31.0 34.0 30.0 34.0 4 35.5022604583924 37.0 35.0 37.0 33.0 37.0 5 35.15502725652044 37.0 35.0 37.0 33.0 37.0 6 35.38869873853025 37.0 35.0 37.0 33.0 37.0 7 35.23160460895026 37.0 35.0 37.0 32.0 37.0 8 35.317304762199186 37.0 35.0 37.0 32.0 37.0 9 36.94868737367269 39.0 37.0 39.0 33.0 39.0 10-11 36.82908623306496 39.0 37.0 39.0 32.0 39.0 12-13 36.7570894187582 39.0 37.0 39.0 32.0 39.0 14-15 37.737091244201636 40.0 37.0 41.0 32.0 41.0 16-17 37.69786776428815 39.5 37.0 41.0 32.0 41.0 18-19 37.975337375807285 40.0 37.0 41.0 32.5 41.0 20-21 38.05788495878767 40.0 37.0 41.0 33.0 41.0 22-23 37.89155761850293 40.0 37.0 41.0 32.5 41.0 24-25 37.78520124777892 40.0 37.0 41.0 32.0 41.0 26-27 37.52696577440758 40.0 36.0 41.0 32.0 41.0 28-29 37.299324880352486 39.5 36.0 41.0 32.0 41.0 30-31 36.86333022713522 39.0 35.5 41.0 31.0 41.0 32-33 36.30187093231579 38.0 35.0 40.5 30.5 41.0 34-35 35.85756535897198 38.0 35.0 40.0 30.0 41.0 36-37 35.44714605166888 38.0 35.0 40.0 28.5 41.0 38-39 35.25721823029147 38.0 35.0 40.0 27.0 41.0 40-41 35.06569020163789 38.0 35.0 41.0 25.5 41.0 42-43 34.738488297288185 38.0 35.0 41.0 24.5 41.0 44-45 34.19063665284335 38.0 34.0 41.0 20.5 41.0 46-47 33.34144183533617 38.0 33.0 41.0 7.5 41.0 48-49 32.63148418856831 38.0 33.0 40.5 2.0 41.0 50-51 32.34105245647274 38.0 32.5 40.0 2.0 41.0 52-53 32.196267557011986 38.0 32.0 40.0 2.0 41.0 54-55 32.01161511997138 37.0 32.0 40.0 2.0 41.0 56-57 31.811776171191262 37.0 31.5 40.0 2.0 41.0 58-59 31.59023505528591 37.0 31.0 40.0 2.0 41.0 60-61 31.35450626907331 36.0 31.0 40.0 2.0 41.0 62-63 31.13869174590416 36.0 31.0 40.0 2.0 41.0 64-65 30.88966224879911 35.0 31.0 39.0 2.0 41.0 66-67 30.61277928180578 35.0 30.5 39.0 2.0 41.0 68-69 30.319983306025286 35.0 30.0 39.0 2.0 41.0 70-71 29.991986450498832 35.0 30.0 38.0 2.0 40.0 72-73 29.730241414895325 35.0 29.5 37.0 2.0 40.0 74-75 29.531642737164116 35.0 30.0 37.0 2.0 39.5 76-77 29.243965363682804 35.0 29.0 36.5 2.0 39.0 78-79 28.930201078483762 35.0 29.0 36.0 2.0 39.0 80-81 28.649743039392774 35.0 29.0 36.0 2.0 37.5 82-83 28.388545636822137 35.0 29.0 35.0 2.0 37.0 84-85 28.129607956578 34.0 29.0 35.0 2.0 37.0 86-87 27.928477948201568 34.0 29.0 35.0 2.0 36.0 88-89 27.741231614913872 34.0 28.5 35.0 2.0 36.0 90-91 27.576800380398858 34.0 27.5 35.0 2.0 36.0 92-93 27.423261449137108 34.0 27.0 35.0 2.0 35.5 94-95 27.275147323590765 34.0 27.0 35.0 2.0 35.0 96-97 27.183454121748245 34.0 27.0 35.0 2.0 35.0 98-99 27.06970544114638 34.0 26.5 35.0 2.0 35.0 100-101 26.947078333605678 34.0 26.0 35.0 2.0 35.0 102-103 25.983565120486627 32.5 24.5 34.5 2.0 35.0 104-105 26.630084191218145 34.0 25.0 35.0 2.0 35.0 106-107 26.744707318114436 34.0 25.0 35.0 2.0 35.0 108-109 26.6825598016744 34.0 25.0 35.0 2.0 35.0 110-111 26.598043242399754 34.0 25.0 35.0 2.0 35.0 112-113 26.480977112766766 34.0 25.0 35.0 2.0 35.0 114-115 26.418256937053116 34.0 25.0 35.0 2.0 35.0 116-117 26.294975466923407 34.0 24.0 35.0 2.0 35.0 118-119 26.13589911186288 34.0 23.5 35.0 2.0 35.0 120-121 25.907563371594037 34.0 21.5 35.0 2.0 35.0 122-123 25.657655747865043 34.0 17.0 35.0 2.0 35.0 124-125 23.810684585455043 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04189032454721442 1101 2 0.5799180025247104 1101 3 0.3549289579640664 1101 4 0.1764972626067376 1101 5 0.45936414587116303 1101 6 0.21533652712884788 1101 7 0.4784649079933345 1101 8 0.37276497155464483 1101 9 0.18515898240382 1101 10-11 0.22507551478702226 1101 12-13 0.19021302803452045 1101 14-15 0.29134772385294383 1101 16-17 0.3867315419035364 1101 18-19 0.31938940561794027 1101 20-21 0.13693073743129247 1101 22-23 -0.09551777308588072 1101 24-25 -0.07583542426434065 1101 26-27 0.15933220724253516 1101 28-29 0.12567233426786117 1101 30-31 -0.1323853310891394 1101 32-33 -0.1294426776364901 1101 34-35 -0.29509426306258746 1101 36-37 -0.36421042993961095 1101 38-39 -0.10624988846036132 1101 40-41 -0.16501264974761654 1101 42-43 -0.1530453301672452 1101 44-45 0.07441290990033878 1101 46-47 0.09683848718211152 1101 48-49 0.22319693773531668 1101 50-51 0.05942939856029028 1101 52-53 0.08602189796449267 1101 54-55 0.12448526279298733 1101 56-57 0.1446713777752855 1101 58-59 0.2393950738347641 1101 60-61 0.25508196537463945 1101 62-63 0.08725939181698195 1101 64-65 0.09106332841562548 1101 66-67 0.1722208426491072 1101 68-69 0.302448202193375 1101 70-71 0.20437110147170756 1101 72-73 0.06991007721158837 1101 74-75 0.06091872941137311 1101 76-77 0.17480128319152044 1101 78-79 0.16060053110797767 1101 80-81 0.019881045435518274 1101 82-83 0.0836853112674909 1101 84-85 0.18424401282358005 1101 86-87 0.29994735129473327 1101 88-89 0.25118205095965607 1101 90-91 0.24143320155458525 1101 92-93 0.08553373429182187 1101 94-95 0.08302402891115435 1101 96-97 0.13697064742822107 1101 98-99 0.11041980151684783 1101 100-101 0.20791327430002937 1101 102-103 0.22049999456783453 1101 104-105 0.05553657535836365 1101 106-107 0.26867700524356053 1101 108-109 0.2220470233267413 1101 110-111 0.19213804071512186 1101 112-113 0.3075427992500721 1101 114-115 0.2196947953939734 1101 116-117 0.48766548338349125 1101 118-119 0.5376917226296918 1101 120-121 0.5941753452683898 1101 122-123 0.42431391305369104 1101 124-125 0.22061257183017702 1102 1 0.4107662004373154 1102 2 0.7539714016179033 1102 3 0.5416054791346667 1102 4 0.2597032691164003 1102 5 0.4107371435828284 1102 6 0.33589134043810986 1102 7 0.4758407909900484 1102 8 0.5211990292887734 1102 9 0.537480002344978 1102 10-11 0.558987741004735 1102 12-13 0.5047339712242547 1102 14-15 0.4880969812118465 1102 16-17 0.7331203162969828 1102 18-19 0.6131314995105157 1102 20-21 0.5699251445265361 1102 22-23 0.5319126887018584 1102 24-25 0.4149401915237121 1102 26-27 0.6146502637259061 1102 28-29 0.7509921318715058 1102 30-31 0.5386148892158786 1102 32-33 0.743410236785671 1102 34-35 0.8277195649857916 1102 36-37 0.6878668331614293 1102 38-39 0.6753558863737013 1102 40-41 0.6807933823458541 1102 42-43 0.5720664213286994 1102 44-45 0.7880554756720457 1102 46-47 0.8239329838206828 1102 48-49 0.776069253212107 1102 50-51 0.6796786274927342 1102 52-53 0.7977812111813805 1102 54-55 0.6676383960988375 1102 56-57 0.37152780286447395 1102 58-59 0.48211257173831257 1102 60-61 0.4781212507335937 1102 62-63 0.6034580287685323 1102 64-65 0.6645412694875823 1102 66-67 0.7171213145282387 1102 68-69 0.7967327083548099 1102 70-71 0.6564636437915929 1102 72-73 0.8179095655354232 1102 74-75 0.9106316222294311 1102 76-77 0.8752015631920926 1102 78-79 0.7869490110033688 1102 80-81 0.7814612719943561 1102 82-83 0.9547748972361916 1102 84-85 1.0320745414134898 1102 86-87 0.8972662037187966 1102 88-89 0.772958491114867 1102 90-91 0.6837603687163458 1102 92-93 0.848955462051677 1102 94-95 0.766035314213255 1102 96-97 0.8330199033657983 1102 98-99 0.8997069371252451 1102 100-101 0.9314438043112574 1102 102-103 0.9792926520148555 1102 104-105 0.9238554704221116 1102 106-107 0.7824060940335862 1102 108-109 0.7323276279574138 1102 110-111 0.8251036660260347 1102 112-113 0.7684777453108751 1102 114-115 0.8353282789137353 1102 116-117 0.8620951776569825 1102 118-119 0.8524187576084437 1102 120-121 0.8426269918707163 1102 122-123 0.7304259559207829 1102 124-125 0.8157416989959181 1103 1 0.02674899834874722 1103 2 0.5201226411431392 1103 3 0.21403915019059028 1103 4 0.18576072812114575 1103 5 0.3468021748160268 1103 6 0.07136426297655163 1103 7 0.14559270608036456 1103 8 0.06901648282568829 1103 9 0.21050362374074894 1103 10-11 0.2784578109174447 1103 12-13 0.19638509473791288 1103 14-15 0.16037552050919857 1103 16-17 0.37630198323044084 1103 18-19 0.2926099510347626 1103 20-21 0.1996337306421978 1103 22-23 0.25695266323327104 1103 24-25 0.3120693996412811 1103 26-27 0.29411567518928194 1103 28-29 6.562378898991028E-4 1103 30-31 0.04893991947781018 1103 32-33 0.2403192927032478 1103 34-35 0.04262876119643977 1103 36-37 0.1317887258947863 1103 38-39 0.09216524428110517 1103 40-41 -0.07498471358942282 1103 42-43 0.023879349514835724 1103 44-45 -0.13507966072699418 1103 46-47 -0.1424641283004604 1103 48-49 -0.26701780583149315 1103 50-51 -0.28129247033650273 1103 52-53 -0.3855071864019841 1103 54-55 -0.45162824886437036 1103 56-57 -0.5384584556093195 1103 58-59 -0.32247672030329255 1103 60-61 -0.5050381851443824 1103 62-63 -0.5397155443340509 1103 64-65 -0.4555406041083607 1103 66-67 -0.3559965550315809 1103 68-69 -0.4210866697152227 1103 70-71 -0.5017689513244115 1103 72-73 -0.2982337600218372 1103 74-75 -0.2582295218065873 1103 76-77 -0.1734693398456102 1103 78-79 -0.19861339333204953 1103 80-81 -0.09599677947880991 1103 82-83 -0.13159122450581506 1103 84-85 -0.11561943623674509 1103 86-87 -0.2764840638272794 1103 88-89 -0.22827986501009434 1103 90-91 -0.13364325624071327 1103 92-93 -0.25896318235755444 1103 94-95 0.006055620735622824 1103 96-97 -0.013685637670828754 1103 98-99 -0.09480454622215007 1103 100-101 -0.2115382147937801 1103 102-103 -0.04988785660147599 1103 104-105 4.07822219575138E-4 1103 106-107 -0.11947239418269362 1103 108-109 -0.058495406725551646 1103 110-111 -0.08841869722869689 1103 112-113 -0.2970085410416665 1103 114-115 -0.22196030572704828 1103 116-117 -0.2634594745870764 1103 118-119 -0.30709805867418893 1103 120-121 -0.2962141334235824 1103 122-123 -0.2447687007119299 1103 124-125 -0.2695704261023302 1104 1 0.2416373556764384 1104 2 0.4115549219260153 1104 3 0.23950488375986367 1104 4 0.12226179074952626 1104 5 0.31024953691887447 1104 6 0.15501223509033935 1104 7 0.2698446423760146 1104 8 0.1736591462437076 1104 9 0.3395929645554574 1104 10-11 0.3832258555923005 1104 12-13 0.28716334775906915 1104 14-15 0.30428163573545675 1104 16-17 0.40590974441916927 1104 18-19 0.1887546996942504 1104 20-21 0.2835586975184228 1104 22-23 0.21025659273521313 1104 24-25 0.07160326591905886 1104 26-27 -0.06891686169147704 1104 28-29 0.10456173065603025 1104 30-31 0.23311164733823375 1104 32-33 0.1892691135236504 1104 34-35 0.37318888639985204 1104 36-37 0.4674739211249701 1104 38-39 0.45268570988128687 1104 40-41 0.3355828811356716 1104 42-43 0.2369843585405107 1104 44-45 0.4626990458008393 1104 46-47 0.42322542415070075 1104 48-49 0.2830671155162179 1104 50-51 0.4484726843448428 1104 52-53 0.5191608200360278 1104 54-55 0.5804142207017762 1104 56-57 0.5698918471730785 1104 58-59 0.6451940338516309 1104 60-61 0.5518337748047593 1104 62-63 0.45208354331722944 1104 64-65 0.6123346726640939 1104 66-67 0.5684781330714266 1104 68-69 0.5651089428300153 1104 70-71 0.6989375409348533 1104 72-73 0.5709535738035356 1104 74-75 0.6113895325875376 1104 76-77 0.6500129689479692 1104 78-79 0.5300615668749664 1104 80-81 0.409832559735289 1104 82-83 0.3046079449545118 1104 84-85 0.44451999483468185 1104 86-87 0.39577600001934954 1104 88-89 0.4062853465818925 1104 90-91 0.5129452175759504 1104 92-93 0.6288045866563792 1104 94-95 0.7123455804535581 1104 96-97 0.6104097943609723 1104 98-99 0.4617601851863 1104 100-101 0.5999737408870587 1104 102-103 0.5552638902884439 1104 104-105 0.5497595744448667 1104 106-107 0.48407385015958937 1104 108-109 0.4371153600564206 1104 110-111 0.4480467962488106 1104 112-113 0.7499080581918172 1104 114-115 0.5095637434650833 1104 116-117 0.44640696325138407 1104 118-119 0.4292980749591102 1104 120-121 0.3905661470391699 1104 122-123 0.2207003230038005 1104 124-125 0.1307559208569522 1105 1 0.10140717222195761 1105 2 0.5422056661281616 1105 3 0.10522831747395145 1105 4 0.08043070732965418 1105 5 0.182999623454009 1105 6 0.15009703952093645 1105 7 0.23285087290561535 1105 8 0.07574464295537098 1105 9 0.11384876240938979 1105 10-11 0.08596036580344446 1105 12-13 0.08339041594874885 1105 14-15 0.0557657132709366 1105 16-17 0.11656654608064088 1105 18-19 0.0927742566343639 1105 20-21 0.13280584185903166 1105 22-23 0.1161885761204644 1105 24-25 -0.05575048970572283 1105 26-27 0.007692691787234196 1105 28-29 0.08594802349091424 1105 30-31 -0.05903528931804658 1105 32-33 -0.20130288370722127 1105 34-35 -0.20404372003836357 1105 36-37 -0.2068900456455438 1105 38-39 -0.11366077679229392 1105 40-41 0.06896813554896397 1105 42-43 0.0939158227149619 1105 44-45 0.29469973808190275 1105 46-47 0.18862002435838576 1105 48-49 0.027823086440413647 1105 50-51 -0.0732216735644684 1105 52-53 -0.04633329557490384 1105 54-55 -0.09086909532453546 1105 56-57 -0.10953009813671954 1105 58-59 -0.043123723157712845 1105 60-61 -0.20427127884702756 1105 62-63 0.04015900195343747 1105 64-65 0.01963823791158603 1105 66-67 0.09227096796239209 1105 68-69 0.16398398609758402 1105 70-71 0.2752009538805069 1105 72-73 0.28424104351623924 1105 74-75 0.2633971476792638 1105 76-77 0.2671815393875683 1105 78-79 0.2626333910355747 1105 80-81 0.20243813259786947 1105 82-83 0.2185920224899931 1105 84-85 0.2617015455442733 1105 86-87 0.41670452199927155 1105 88-89 0.5422882887764224 1105 90-91 0.30737235499374194 1105 92-93 0.32994685041546745 1105 94-95 0.3504599798997319 1105 96-97 0.2549148133987025 1105 98-99 0.2957511855324384 1105 100-101 0.27582611194971207 1105 102-103 0.09552436103957263 1105 104-105 0.2577121531918358 1105 106-107 0.13528101526861747 1105 108-109 0.31247448085946417 1105 110-111 0.28845870348315117 1105 112-113 0.28629175497270865 1105 114-115 0.35789794214975146 1105 116-117 0.3197108746394086 1105 118-119 0.09406529545063336 1105 120-121 0.31210906846526854 1105 122-123 0.25072368748978135 1105 124-125 0.44154685197498367 1106 1 0.15285230894746604 1106 2 0.6330502490288197 1106 3 0.27969179964771484 1106 4 0.1400188000953193 1106 5 0.3766046771057887 1106 6 0.326974851912766 1106 7 0.42872314704891323 1106 8 0.2231918565240818 1106 9 0.25174249726573805 1106 10-11 0.3369641733493154 1106 12-13 0.28949979635719814 1106 14-15 0.21082369181022642 1106 16-17 0.21619011825094248 1106 18-19 0.3139883445540619 1106 20-21 0.21066150125674454 1106 22-23 0.2214715460062351 1106 24-25 0.20664999489102343 1106 26-27 0.4142607084019829 1106 28-29 0.43773930074948453 1106 30-31 0.4190929557494485 1106 32-33 0.343007431280661 1106 34-35 0.4390767368671433 1106 36-37 0.5216795286016023 1106 38-39 0.37978851361960864 1106 40-41 0.3421249372104356 1106 42-43 0.4276385641479834 1106 44-45 0.5024186719690604 1106 46-47 0.47509458302920393 1106 48-49 0.41063720897415834 1106 50-51 0.30641660957848416 1106 52-53 0.3986000723724814 1106 54-55 0.5168628188326139 1106 56-57 0.5801722210048545 1106 58-59 0.5437921646927464 1106 60-61 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-0.5019411947316925 2216 14-15 -0.5707868276420669 2216 16-17 -0.5866440482345965 2216 18-19 -0.9003889647265311 2216 20-21 -0.45240087648722493 2216 22-23 -0.631647341192199 2216 24-25 -0.5002767601622224 2216 26-27 -0.9902013650603081 2216 28-29 -1.5922302710827267 2216 30-31 -1.3822458836332885 2216 32-33 -1.08878565717837 2216 34-35 -1.3051419112528393 2216 36-37 -0.9982530947411519 2216 38-39 -0.9636650834245089 2216 40-41 -0.6876468515311416 2216 42-43 -0.5216268129439499 2216 44-45 -0.930528513425422 2216 46-47 -0.3595065906286905 2216 48-49 -0.3674740711823219 2216 50-51 -0.26707610939565285 2216 52-53 -0.3602501775731852 2216 54-55 -0.42911414269748605 2216 56-57 -0.8907015020988176 2216 58-59 -1.0621934905995758 2216 60-61 -0.3242562056342777 2216 62-63 -0.45599948211202346 2216 64-65 -0.3219035364208871 2216 66-67 -0.29212825684381016 2216 68-69 -0.22336312845883555 2216 70-71 -0.128567349306401 2216 72-73 -0.11841830187566416 2216 74-75 -0.3757415258780128 2216 76-77 -0.13601398171493173 2216 78-79 -0.4168325865699387 2216 80-81 -0.2450446408732745 2216 82-83 -0.14953028586487704 2216 84-85 -0.27545392387864 2216 86-87 -0.30347850986980873 2216 88-89 -0.3391178266413206 2216 90-91 -0.31264487153511666 2216 92-93 -0.45852860178091603 2216 94-95 -0.31502020959208465 2216 96-97 -0.4272341960758901 2216 98-99 -0.502560614639826 2216 100-101 -0.5556446029512365 2216 102-103 -0.5454424778923865 2216 104-105 -0.5311293627426998 2216 106-107 -0.4197383645763395 2216 108-109 -0.36200656995879754 2216 110-111 -0.47490908290789235 2216 112-113 -0.3018154801694095 2216 114-115 -0.50685442649797 2216 116-117 -0.4156078161443979 2216 118-119 -0.2802607166079376 2216 120-121 -0.3251239203374894 2216 122-123 -0.29445073111486764 2216 124-125 -0.5582421230465506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 55.0 4 133.0 5 369.0 6 906.0 7 1742.0 8 2758.0 9 4561.0 10 8137.0 11 10379.0 12 10800.0 13 14297.0 14 20153.0 15 14708.0 16 5760.0 17 4636.0 18 4638.0 19 4199.0 20 4211.0 21 4123.0 22 4379.0 23 4900.0 24 5753.0 25 6825.0 26 8124.0 27 9371.0 28 11150.0 29 13562.0 30 16760.0 31 20492.0 32 25556.0 33 32121.0 34 43103.0 35 61410.0 36 97790.0 37 143458.0 38 57687.0 39 243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.90342206443439 17.06936935609799 12.704064346007101 18.32314423346051 2 19.537896464665053 23.003599372870003 36.74024893822181 20.718255224243137 3 17.7327370209308 30.40265808510701 33.780175066172426 18.084429827789766 4 10.558570972210568 17.58108133969883 35.350595550923245 36.509752137167354 5 9.279615574987627 43.65100643458175 33.40723477973931 13.662143210691305 6 26.753787427111074 37.58499721031022 18.463035506347097 17.198179856231608 7 23.74504443629865 37.13040290775475 22.30662444592639 16.81792821002021 8 29.22431902862855 31.330498007469597 20.511065278740798 18.93411768516106 9 28.2744995855949 10.293881672989473 20.76912998482232 40.66248875659331 10-11 24.74381226197469 26.47710025364831 27.88247088491315 20.89661659946385 12-13 27.20735123740039 26.786174326904533 29.341059081065886 16.665415354629193 14-15 23.25938815257763 26.09405155700021 31.180487776153527 19.466072514268635 16-17 18.734202185526883 28.021808160615468 31.81136986281204 21.432619791045614 18-19 17.947789373269323 22.891723233331422 39.523647589310556 19.636839804088698 20-21 19.82409497016725 21.793880936923568 40.66138465773672 17.720639435172465 22-23 19.38746067567906 22.57742309215398 40.118683266424945 17.916432965742022 24-25 18.266947549415786 22.913216357739806 40.78445487695186 18.035381215892546 26-27 18.466053376555124 22.600756381323357 40.62980743043809 18.303382811683427 28-29 18.365533810501024 22.376730861522244 40.82507220817152 18.432663119805206 30-31 18.356085130438238 22.34313331478447 40.93468592803926 18.366095626738037 32-33 17.92887247952633 22.746791856755685 40.33059664030078 18.9937390234172 34-35 18.552006889597152 22.690549622029046 40.16215579616803 18.595287692205776 36-37 19.140731384525246 22.731040046401592 38.778160041337465 19.3500685277357 38-39 19.19939583710567 22.943542273000954 38.60231389677706 19.25474799311631 40-41 19.042775029387453 23.240414758721098 38.19492953267877 19.521880679212675 42-43 19.237051404259702 23.42822652175673 37.501959817304545 19.832762256679022 44-45 19.172221383851507 23.770901502074047 36.810052596515895 20.24682451755855 46-47 20.020288022190208 24.17241285389017 35.00927904643351 20.79802007748611 48-49 20.31738950883245 24.758310347695954 33.29821996574054 21.62608017773106 50-51 20.475578444016513 25.3673980766626 32.632914038714084 21.5241094406068 52-53 20.586035610283226 25.46735920334726 32.28429631854331 21.662308867826194 54-55 20.34573910124617 25.68044749981781 32.35172225448865 21.622091144447364 56-57 20.606939032999165 25.53918360766999 32.00953412210183 21.844343237229012 58-59 20.77687769365815 24.77153362311053 32.476343654652325 21.975245028579 60-61 21.037301699466486 25.12837272845046 32.68396165306387 21.150363919019185 62-63 21.153725833698307 24.89748298743886 32.928736782831756 21.020054396031078 64-65 20.822703095809786 24.973611493464336 33.33799518014112 20.865690230584757 66-67 20.949889476694413 25.478216640400664 32.71298462978925 20.85890925311567 68-69 21.56471903644823 25.174431400375546 32.39194233293119 20.868907230245032 70-71 21.087921115082704 25.326527968342393 32.40826890122897 21.17728201534593 72-73 21.252099689812695 25.408418191962806 31.993899306163847 21.34558281206065 74-75 20.6735528947258 26.082306447208325 31.899287258466153 21.34485339959972 76-77 20.313110710523855 25.938989857650853 32.219532284527524 21.528367147297768 78-79 20.58256972236187 25.799390092649094 31.88602402384277 21.732016161146266 80-81 20.166469522666162 26.119212375981455 31.564214352328595 22.150103749023785 82-83 20.189552389315523 25.80376852408674 31.43160192820534 22.575077158392396 84-85 20.211119199687317 25.453988719012433 31.537669415392227 22.797222665908027 86-87 20.11819815894112 25.628935215514005 31.19303736439746 23.059829261147417 88-89 20.26969931176622 25.637628353759524 31.317065989474074 22.775606345000185 90-91 20.087696570161672 25.79334701460677 31.148402559202914 22.97055385602864 92-93 20.12641251670877 25.78582743005871 30.980341065003742 23.10741898822878 94-95 19.94765010662095 25.575852507561365 31.19823988941159 23.278257496406095 96-97 20.420625550219793 25.54860041750457 30.901226898129476 23.12954713414616 98-99 20.300335136973345 25.516488547707883 30.469419947311433 23.71375636800734 100-101 20.160508539575446 25.847424878013953 30.62330104662521 23.368765535785396 102-103 20.511952326132594 25.457836362512182 30.549609734984884 23.480601576370344 104-105 20.21876023598429 25.487628490355636 30.734193800075815 23.55941747358426 106-107 20.146257264098516 25.661635622881494 30.509176962456706 23.682930150563276 108-109 20.30389597197615 25.84889702824394 29.673616933260803 24.173590066519107 110-111 20.146743394854266 26.00668942602546 29.855980073047828 23.99058710607245 112-113 20.12024325444471 25.61930723572286 29.807429699215188 24.453019810617242 114-115 19.577992160881564 26.08700773604964 29.722170687822274 24.612829415246527 116-117 19.657316767022078 25.87476322184668 29.850501237172754 24.617418773958487 118-119 19.645558550049728 25.92926041228811 29.396820863076854 25.028360174585305 120-121 19.589499502387948 26.045426974695395 29.222635753867536 25.14243776904912 122-123 19.55173198142326 26.25911593384631 28.92078090620866 25.268371178521765 124-125 19.36724095414411 26.232632435158028 28.66267450377072 25.73745210692715 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 255.0 1 223.5 2 767.5 3 1424.5 4 1086.0 5 921.0 6 1422.0 7 3626.0 8 5718.5 9 6173.5 10 5103.5 11 3311.5 12 2777.0 13 2671.5 14 2700.0 15 2792.0 16 2919.5 17 3104.0 18 3389.0 19 3495.5 20 3569.0 21 3845.0 22 4262.5 23 4879.5 24 5567.5 25 6123.5 26 6745.0 27 7466.5 28 8230.5 29 9095.5 30 10223.0 31 11353.5 32 12823.0 33 14176.5 34 15408.5 35 15955.5 36 16656.5 37 18312.5 38 18823.5 39 18522.5 40 17853.0 41 18120.0 42 18539.5 43 18811.0 44 20091.5 45 20901.5 46 20706.0 47 20389.0 48 20355.0 49 19979.5 50 19661.5 51 18541.0 52 17185.5 53 16705.5 54 16144.0 55 15352.0 56 14424.0 57 13354.5 58 12903.0 59 12493.5 60 11534.5 61 10612.0 62 9317.5 63 6852.0 64 4706.0 65 3912.5 66 3002.5 67 2326.0 68 1843.0 69 1251.5 70 960.5 71 839.5 72 830.0 73 930.5 74 742.0 75 484.5 76 325.0 77 247.0 78 168.5 79 52.5 80 22.0 81 8.0 82 6.5 83 3.5 84 0.5 85 2.5 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06727642366186899 2 2.9442636175872647E-4 3 1.4721318087936323E-4 4 0.0 5 0.06698199730011026 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.4721318087936323E-4 30-31 0.0 32-33 0.0 34-35 2.9442636175872647E-4 36-37 0.0 38-39 0.0 40-41 7.360659043968162E-5 42-43 0.002134591122750767 44-45 0.008759184262322112 46-47 0.01008410289023638 48-49 0.008611971081442748 50-51 0.007507872224847523 52-53 0.006624593139571345 54-55 0.0068454129108903895 56-57 0.006256560187372937 58-59 0.004342788835941215 60-61 0.0022081977131904484 62-63 0.0013985252183539504 64-65 0.002060984532311085 66-67 0.0027234438462682194 68-69 0.0012513120374745873 70-71 0.002797050436707901 72-73 0.0029442636175872642 74-75 0.0012513120374745873 76-77 9.568856757158609E-4 78-79 5.152461330777713E-4 80-81 5.152461330777713E-4 82-83 3.6803295219840803E-4 84-85 3.6803295219840803E-4 86-87 5.888527235174529E-4 88-89 0.0017665581705523586 90-91 0.0019137713514317217 92-93 4.4163954263808965E-4 94-95 0.001692951580112677 96-97 0.0019137713514317217 98-99 0.00184016476099204 100-101 0.0012513120374745873 102-103 7.360659043968161E-4 104-105 6.624593139571345E-4 106-107 6.624593139571345E-4 108-109 0.0012513120374745873 110-111 8.832790852761793E-4 112-113 0.011850661060788739 114-115 0.018990500333437854 116-117 0.017297548753325176 118-119 0.01140902151815065 120-121 0.006330166777812618 122-123 0.007434265634407843 124-125 0.005814920644734847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 679287.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.32807556205836 #Duplication Level Percentage of deduplicated Percentage of total 1 54.30371775421043 16.469272553503842 2 13.98411770162255 8.482227566470533 3 9.354899842083912 8.511483278586262 4 6.375755084725506 7.7345752789893165 5 4.342802351834222 6.585441893875653 6 2.931372020672088 5.334172328606807 7 1.98593647902017 4.216074211802029 8 1.368787211807639 3.3210145590465006 9 0.9813849710557602 2.6787165801882797 >10 4.071995734167187 20.73647800300992 >50 0.19399082597329084 4.053935013291697 >100 0.09602545861664243 5.07514067791212 >500 0.0048497706372041625 0.9053033146659645 >1k 0.0029098623823224975 1.6841142084362781 >5k 9.699541274408325E-4 2.595742144147025 >10k+ 4.8497706372041625E-4 1.6163083874677957 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10989 1.6177256446833224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9037 1.3303655156068053 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8611 1.2676527005521967 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2601 0.3829014834672237 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2217 0.3263716220095482 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 0.27867455140463454 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1723 0.2536483106551428 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1680 0.2473181438773302 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1336 0.19667680965482925 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 889 0.1308725178017539 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 858 0.12630890919449364 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 708 0.10422693206258915 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.472131808793632E-4 0.0 0.0 0.0 3 0.0 1.472131808793632E-4 0.0 0.0 0.0 4 0.0 1.472131808793632E-4 0.0 0.0 0.0 5 0.0 7.360659043968161E-4 0.0 0.0 0.0 6 0.0 7.360659043968161E-4 0.0 0.0 0.0 7 0.0 7.360659043968161E-4 0.0 0.0 0.0 8 0.0 7.360659043968161E-4 0.0 0.0 0.0 9 0.0 7.360659043968161E-4 0.0 0.0 0.0 10-11 0.0 7.360659043968161E-4 0.0 0.0 0.0 12-13 0.0 7.360659043968161E-4 0.0 0.0 0.0 14-15 0.0 7.360659043968161E-4 0.0 0.0 0.0 16-17 0.0 7.360659043968161E-4 0.0 0.0 0.0 18-19 0.0 7.360659043968161E-4 0.0 0.0 0.0 20-21 0.0 7.360659043968161E-4 0.0 0.0 0.0 22-23 0.0 7.360659043968161E-4 0.0 2.2081977131904482E-4 0.0 24-25 0.0 7.360659043968161E-4 0.0 4.4163954263808965E-4 0.0 26-27 0.0 7.360659043968161E-4 0.0 0.0010304922661555425 0.0 28-29 0.0 7.360659043968161E-4 0.0 0.0025026240749491746 0.0 30-31 0.0 7.360659043968161E-4 0.0 0.007949511767485614 0.0 32-33 0.0 7.360659043968161E-4 0.0 0.017739188295963267 0.0 34-35 0.0 7.360659043968161E-4 0.0 0.03150362070818373 0.0 36-37 0.0 7.360659043968161E-4 0.0 0.05572018896283898 0.0 38-39 0.0 0.0010304922661555425 0.0 0.09274430395399882 0.0 40-41 0.0 0.0010304922661555425 0.0 0.1372762911700062 0.0 42-43 0.0 0.0010304922661555425 0.0 0.18725516607855 0.0 44-45 0.0 0.0010304922661555425 0.0 0.24628765161117466 0.0 46-47 0.0 0.0010304922661555425 0.0 0.31952620909865787 0.0 48-49 0.0 0.0010304922661555425 0.0 0.3934272259000982 0.0 50-51 0.0 0.0010304922661555425 0.0 0.4692420140529702 0.0 52-53 0.0 0.0010304922661555425 0.0 0.5570546764475104 0.0 54-55 0.0 0.0010304922661555425 0.0 0.6601775096535043 0.0 56-57 0.0 0.0010304922661555425 0.0 0.7956136360625186 0.0 58-59 0.0 0.0010304922661555425 0.0 0.9546038714122308 0.0 60-61 0.0 0.0010304922661555425 0.0 1.1080736124789667 0.0 62-63 0.0 0.0010304922661555425 0.0 1.261322533774384 0.0 64-65 0.0 0.0010304922661555425 0.0 1.4256860502261932 0.0 66-67 0.0 0.0010304922661555425 0.0 1.6423838524806156 0.0 68-69 0.0 0.0010304922661555425 0.0 1.9004485585621393 0.0 70-71 0.0 0.0010304922661555425 0.0 2.1589549041863014 0.0 72-73 0.0 0.0010304922661555425 0.0 2.4111310830326502 0.0 74-75 0.0 0.0010304922661555425 0.0 2.6889959619424486 0.0 76-77 0.0 0.0010304922661555425 0.0 3.027954310917182 0.0 78-79 0.0 0.0010304922661555425 0.0 3.4537684366107406 0.0 80-81 0.0 0.0010304922661555425 0.0 3.8715594439463734 0.0 82-83 0.0 0.0010304922661555425 0.0 4.279707987934407 0.0 84-85 0.0 0.0010304922661555425 0.0 4.697351782089161 0.0 86-87 0.0 0.0010304922661555425 0.0 5.164827237971579 0.0 88-89 0.0 0.0010304922661555425 0.0 5.729831426186575 0.0 90-91 0.0 0.0010304922661555425 0.0 6.318316116751829 0.0 92-93 0.0 0.0010304922661555425 0.0 6.889429651973319 0.0 94-95 0.0 0.0010304922661555425 0.0 7.516852228881165 0.0 96-97 0.0 0.0010304922661555425 0.0 8.174527114459721 0.0 98-99 0.0 0.0010304922661555425 0.0 8.88188644858506 0.0 100-101 7.36065904396816E-5 0.0010304922661555425 0.0 9.65850958431414 0.0 102-103 1.472131808793632E-4 0.0010304922661555425 0.0 10.409664839751091 0.0 104-105 1.472131808793632E-4 0.0010304922661555425 0.0 11.15912714360793 0.0 106-107 1.472131808793632E-4 0.0010304922661555425 0.0 11.955329632393966 0.0 108-109 1.472131808793632E-4 0.0011777054470349056 0.0 12.818587725070552 0.0 110-111 1.472131808793632E-4 0.0011777054470349056 0.0 13.758249458623528 0.0 112-113 1.472131808793632E-4 0.001324918627914269 0.0 14.632033293732988 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4465 0.0 91.67324 1 TATAACG 70 1.3340468E-8 59.537052 2 GTATCAA 7710 0.0 53.204964 2 GTACATG 8260 0.0 44.288975 1 TACATGG 8415 0.0 43.016666 2 ACATGGG 8340 0.0 42.3327 3 ATGGGCG 170 0.0 42.026157 5 ATGGGAT 2270 0.0 41.439888 5 CATGGGA 6595 0.0 40.533947 4 CGGGAGT 610 0.0 40.01671 4 ATGGGAG 2000 0.0 39.889824 5 ATCAACG 11295 0.0 37.530216 4 TCAACGC 11305 0.0 37.444355 5 AACGCGG 80 1.1925765E-4 37.21066 5 CAACGCA 11455 0.0 37.13731 6 GTGGTAT 1080 0.0 36.96225 1 GTCGGGA 680 0.0 36.772884 2 CAACGCG 65 0.0019324268 36.638184 4 AACGCAG 11605 0.0 36.606026 7 TGGTATC 1095 0.0 36.42906 2 >>END_MODULE