FastQCFastQC Report
Thu 26 May 2016
SRR936195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269389
Sequences flagged as poor quality0
Sequence length125
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14880.5523610837859007No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5210.19340062140621925No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5040.18709004450812766No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG4650.17261283868309396No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4330.16073410569845092No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.15219626636573877No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3650.13549179810608453No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT3580.13289332526569386No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3130.11618885700603958No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA2990.11099191132525826No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2780.1031964928040863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT454.0563464E-1092.545451
GTACATA3050.091.678511
GTTCGAC150.004221609359.54877516-17
CATGGGT2350.055.696054
TACATAA3350.051.501842
GTCGGGA4000.050.569482
GTACATG47700.050.139181
CATTCCG505.328157E-447.5948039
GGGATAG1004.130925E-947.5948037
TACATGG47950.046.8999862
CGGGAGT4200.046.7448964
GTCACTA1301.8189894E-1145.764231
ATGGGAG10800.044.069265
CATATAG951.483113E-743.837323
ACGCAAA1501.8189894E-1243.628571
ACATGGG48750.043.567553
TCGGGAG4550.043.149133
CATGGGG8150.043.068924
ACTACCT1404.0017767E-1142.4953584
TGGGAGT2400.042.1412356
Produced by FastQC (version 0.11.5)