##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 269389 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.141434876702462 31.0 26.0 31.0 12.0 34.0 2 27.41330937788848 31.0 26.0 33.0 12.0 34.0 3 27.557617423131607 31.0 26.0 34.0 12.0 34.0 4 30.15966873183389 35.0 30.0 37.0 7.0 37.0 5 31.31183158926311 35.0 31.0 37.0 12.0 37.0 6 30.543039990497014 35.0 31.0 37.0 8.0 37.0 7 30.650642750817592 35.0 31.0 37.0 8.0 37.0 8 30.19012283352327 35.0 30.0 37.0 8.0 37.0 9 31.035075671241216 38.0 30.0 39.0 8.0 39.0 10-11 31.326537089487694 37.0 30.0 39.0 8.0 39.0 12-13 31.29107164732042 37.0 30.5 39.0 7.5 39.0 14-15 32.23686379176581 38.0 30.5 41.0 8.0 41.0 16-17 32.425403412908466 38.0 31.0 41.0 8.0 41.0 18-19 32.61563018534535 38.5 30.5 41.0 8.0 41.0 20-21 32.934791324070396 39.0 30.5 41.0 11.0 41.0 22-23 33.06325796524728 38.5 30.0 41.0 13.0 41.0 24-25 33.25362950974242 38.0 30.0 41.0 16.5 41.0 26-27 33.49194473419479 38.0 30.0 41.0 18.0 41.0 28-29 33.42121244742732 38.0 30.0 40.0 18.0 41.0 30-31 33.30890088310956 38.0 30.0 40.0 17.0 41.0 32-33 33.128535315101956 38.0 30.0 40.0 16.0 41.0 34-35 33.017259427816285 38.0 30.0 40.0 15.5 41.0 36-37 32.82898151000968 38.0 30.0 40.0 9.0 41.0 38-39 32.49853928705329 38.0 30.0 40.0 8.0 41.0 40-41 32.529687552201466 38.0 30.0 40.0 4.0 41.0 42-43 32.345730523518036 38.0 30.0 40.0 2.0 41.0 44-45 32.22112632661319 38.0 30.0 40.0 2.0 41.0 46-47 32.177115620905084 38.0 30.0 40.0 2.0 41.0 48-49 32.041308293954096 38.0 30.0 40.0 2.0 41.0 50-51 31.905469414118617 37.0 30.0 40.0 2.0 41.0 52-53 31.72532100419839 37.0 30.0 40.0 2.0 41.0 54-55 31.485090334052245 37.0 30.0 40.0 2.0 41.0 56-57 31.25792070203312 36.5 30.0 40.0 2.0 41.0 58-59 30.9454320703518 36.0 29.5 40.0 2.0 41.0 60-61 30.67226761300573 36.0 29.0 40.0 2.0 41.0 62-63 30.364749488657665 35.0 29.0 39.5 2.0 41.0 64-65 30.033238179732656 35.0 29.0 39.0 2.0 41.0 66-67 29.72688751211074 35.0 28.0 39.0 2.0 41.0 68-69 29.420774419148515 35.0 28.0 39.0 2.0 40.5 70-71 29.057745490721597 35.0 28.0 38.0 2.0 40.0 72-73 28.736626217106117 34.0 27.5 37.5 2.0 40.0 74-75 28.363478093017903 34.0 27.0 37.0 2.0 39.5 76-77 28.029370167304528 34.0 26.5 36.5 2.0 39.0 78-79 27.67363552335099 34.0 26.0 36.0 2.0 39.0 80-81 27.355046048650834 34.0 26.0 36.0 2.0 37.5 82-83 27.026428324838804 34.0 25.5 35.0 2.0 37.0 84-85 26.977116734536303 34.0 26.0 35.0 2.0 37.0 86-87 26.813453778736324 34.0 25.5 35.0 2.0 36.5 88-89 26.609005935654388 34.0 25.0 35.0 2.0 36.0 90-91 26.40879174725026 34.0 25.0 35.0 2.0 36.0 92-93 26.249889564904283 34.0 24.5 35.0 2.0 36.0 94-95 26.086870659158315 34.0 24.0 35.0 2.0 35.0 96-97 25.94103879519951 34.0 23.0 35.0 2.0 35.0 98-99 25.80913845776925 34.0 22.5 35.0 2.0 35.0 100-101 25.676130057277767 34.0 20.0 35.0 2.0 35.0 102-103 24.771013293044632 31.5 19.0 34.0 2.0 35.0 104-105 25.290435021474522 33.0 19.0 35.0 2.0 35.0 106-107 25.367967882875693 33.0 19.0 35.0 2.0 35.0 108-109 25.308448006414515 33.0 18.0 35.0 2.0 35.0 110-111 25.170368871780212 33.0 17.0 35.0 2.0 35.0 112-113 25.10246335225269 33.0 15.5 35.0 2.0 35.0 114-115 24.9447824521417 33.0 8.5 35.0 2.0 35.0 116-117 24.802265497106415 33.0 2.0 35.0 2.0 35.0 118-119 24.59375104402927 33.0 2.0 35.0 2.0 35.0 120-121 24.353947265849754 33.0 2.0 35.0 2.0 35.0 122-123 24.023293452962072 33.0 2.0 35.0 2.0 35.0 124-125 22.102749184265132 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.058140504385651326 1101 2 0.15467673307171736 1101 3 0.4143998562127891 1101 4 0.21394386362828754 1101 5 0.31008400243775114 1101 6 -0.018310745821469965 1101 7 -0.09341581309435298 1101 8 -0.00631337217652117 1101 9 -0.18799395758315995 1101 10-11 -0.13896355763970192 1101 12-13 -0.12861577994425843 1101 14-15 -0.13838205291364147 1101 16-17 -0.20567627323642768 1101 18-19 -0.11157082126969442 1101 20-21 0.025563607688582124 1101 22-23 0.3076095160927821 1101 24-25 0.3188349774509547 1101 26-27 0.07955616505336138 1101 28-29 0.26617529686081554 1101 30-31 0.3878353233015801 1101 32-33 0.274055922610664 1101 34-35 0.12295210092070619 1101 36-37 0.29706637930429025 1101 38-39 0.21445154249770582 1101 40-41 0.1740815528582047 1101 42-43 0.13897257576889643 1101 44-45 0.30780751609197665 1101 46-47 0.2961470055786677 1101 48-49 0.20497092670943573 1101 50-51 0.045854132707226825 1101 52-53 -0.2333568430622499 1101 54-55 0.09795026876120261 1101 56-57 0.010087574565407209 1101 58-59 -0.13427165032976163 1101 60-61 -0.19601941940316792 1101 62-63 0.032612945693198725 1101 64-65 -0.07370900932275148 1101 66-67 0.04323065705433393 1101 68-69 0.06033655344855049 1101 70-71 0.02940237722526362 1101 72-73 -0.11031516221927973 1101 74-75 -0.14593256075714933 1101 76-77 -0.09305051080859172 1101 78-79 -0.13377474057563887 1101 80-81 -0.27953828306301887 1101 82-83 -0.40491293778106296 1101 84-85 -0.595228538458926 1101 86-87 -0.21478482135310983 1101 88-89 -0.4216049690056032 1101 90-91 -0.465042450390758 1101 92-93 -0.32428117883096874 1101 94-95 -0.2567412703133414 1101 96-97 -0.4296561293590493 1101 98-99 -0.3891412996135486 1101 100-101 -0.26874794034456073 1101 102-103 -0.21988776204701566 1101 104-105 -0.34483903207467037 1101 106-107 -0.26997939439451457 1101 108-109 -0.3829948916805037 1101 110-111 -0.5567049432553688 1101 112-113 -0.5070800212577495 1101 114-115 -0.42069834171303455 1101 116-117 -0.3355958867300224 1101 118-119 -0.47322626662956324 1101 120-121 -0.1791349554167887 1101 122-123 -0.335312306429973 1101 124-125 -0.650539503551844 1102 1 0.21469174740949626 1102 2 0.27225664174751785 1102 3 0.23802999319908835 1102 4 0.19935735931575493 1102 5 0.0773658390689036 1102 6 0.19541325214910543 1102 7 0.2982635374891096 1102 8 0.4996797785084084 1102 9 0.12621837118396328 1102 10-11 0.08960062297927252 1102 12-13 0.2501418304058163 1102 14-15 0.1560909303435949 1102 16-17 0.42319232138559215 1102 18-19 0.35870124874551834 1102 20-21 -0.035477741880164615 1102 22-23 0.15663919645807312 1102 24-25 0.4923033944981441 1102 26-27 0.28855540401836066 1102 28-29 0.31727562664265463 1102 30-31 0.4103492756100522 1102 32-33 0.6564443041428873 1102 34-35 0.5331975347106592 1102 36-37 0.5513947659100751 1102 38-39 0.5208949722288025 1102 40-41 0.7612517710215698 1102 42-43 1.0024777761747785 1102 44-45 0.8349986627180499 1102 46-47 0.6266581674254539 1102 48-49 0.5603425654714869 1102 50-51 0.5491836608655234 1102 52-53 0.6686281899159319 1102 54-55 0.8172868999129008 1102 56-57 0.6698256516836203 1102 58-59 0.7115236312531934 1102 60-61 0.748329895665325 1102 62-63 0.8423541937906123 1102 64-65 0.6145837354768418 1102 66-67 0.5753368214378938 1102 68-69 0.5463366802877161 1102 70-71 0.26955648681430233 1102 72-73 0.42607182408208644 1102 74-75 0.5399660947476725 1102 76-77 0.6849492355130735 1102 78-79 0.5978335800737788 1102 80-81 0.7900488554453489 1102 82-83 0.7448048450702842 1102 84-85 0.7784555051737492 1102 86-87 0.9477437174596766 1102 88-89 0.8363858985743029 1102 90-91 1.0476573212987397 1102 92-93 0.9558431235536062 1102 94-95 0.912493889504006 1102 96-97 0.7313186296465339 1102 98-99 0.47420535183241697 1102 100-101 0.5059064229228163 1102 102-103 0.5550202795562313 1102 104-105 0.5640270257639877 1102 106-107 0.6358900881016787 1102 108-109 0.5824414350571914 1102 110-111 0.44584769499932264 1102 112-113 0.2478191416037454 1102 114-115 0.24918686883998475 1102 116-117 -0.029104892310943598 1102 118-119 0.1716240631522723 1102 120-121 0.1818810263183721 1102 122-123 0.34332480671056587 1102 124-125 0.4248505268895535 1103 1 -0.2972578986044958 1103 2 -0.30021623328913805 1103 3 -0.13414754439883225 1103 4 -0.019066669840963613 1103 5 0.06783544654240714 1103 6 -0.29642831292986216 1103 7 0.10315231466643837 1103 8 0.07644656666139937 1103 9 0.09277821367066252 1103 10-11 0.11349991500725665 1103 12-13 -0.43420319074956026 1103 14-15 -0.12618149226123876 1103 16-17 -0.2688346150644918 1103 18-19 -0.02865160618304685 1103 20-21 -0.22250384049355176 1103 22-23 -0.199844612347583 1103 24-25 0.08791627204831087 1103 26-27 -0.21434036909545995 1103 28-29 0.05039845350369632 1103 30-31 0.236246808180951 1103 32-33 0.15476863073163116 1103 34-35 -0.015522669721498517 1103 36-37 -0.2332916737028512 1103 38-39 -0.2985878730646476 1103 40-41 0.0566206286312152 1103 42-43 0.11904180784500085 1103 44-45 0.004152657104548041 1103 46-47 -0.0780672315942823 1103 48-49 -0.25542237944074486 1103 50-51 -0.1136132475170335 1103 52-53 0.08020499859592078 1103 54-55 0.08964666699429813 1103 56-57 -0.036601603144411854 1103 58-59 0.14624567892609974 1103 60-61 0.171526452156467 1103 62-63 0.1865375124618005 1103 64-65 0.39643200324945127 1103 66-67 0.13889197203904047 1103 68-69 0.14041003289129605 1103 70-71 -0.09339961054232049 1103 72-73 -0.006435192800321232 1103 74-75 0.15670293023843485 1103 76-77 -0.19585802015279796 1103 78-79 -0.0348535717003422 1103 80-81 0.10154606963830304 1103 82-83 0.12578490455668856 1103 84-85 0.19708585161922798 1103 86-87 0.15553245693435613 1103 88-89 0.13917484750815845 1103 90-91 0.10844761077131793 1103 92-93 0.34344732949386625 1103 94-95 0.47471897433497645 1103 96-97 0.46523216489851293 1103 98-99 0.5101642559420085 1103 100-101 0.307396724962949 1103 102-103 0.47531058997472897 1103 104-105 0.37317829718118745 1103 106-107 0.38372218562587435 1103 108-109 0.14393341277073546 1103 110-111 0.10479055453600239 1103 112-113 0.3780818034109572 1103 114-115 0.22939297666956548 1103 116-117 0.14069318904299877 1103 118-119 0.2518416912365602 1103 120-121 -0.26304168324553245 1103 122-123 -0.24434433157438562 1103 124-125 -0.29729663912779003 1104 1 -0.08439501058137111 1104 2 -0.15240089249905608 1104 3 -0.10215265520273675 1104 4 0.023558272557764326 1104 5 -0.3906706906114614 1104 6 -0.30686985287372437 1104 7 -0.06931637118669087 1104 8 0.013378408645394302 1104 9 0.2677708825994891 1104 10-11 -0.04396102085634013 1104 12-13 0.1588176294925745 1104 14-15 0.20403613582304558 1104 16-17 0.08186812047235037 1104 18-19 -0.0865042307065309 1104 20-21 0.05356335401024381 1104 22-23 -0.13673979897571087 1104 24-25 -0.4186555096114475 1104 26-27 -0.1612162854793624 1104 28-29 -0.22496181627972334 1104 30-31 -0.45152287050867557 1104 32-33 -0.2659301250808568 1104 34-35 -0.15287552465007082 1104 36-37 0.1404358618004835 1104 38-39 -0.015634708136488484 1104 40-41 -0.1211683203026368 1104 42-43 0.1428887350788841 1104 44-45 0.08385711020663678 1104 46-47 0.05017414916061114 1104 48-49 0.021529780083362482 1104 50-51 -0.06953780032169021 1104 52-53 -0.15785582834890732 1104 54-55 0.022766351967696608 1104 56-57 -0.01053964512003347 1104 58-59 0.03572454449520279 1104 60-61 0.05540752123610204 1104 62-63 0.16769665954403834 1104 64-65 0.2355883016868887 1104 66-67 0.155245497236983 1104 68-69 0.14679330129228418 1104 70-71 0.2684149343028892 1104 72-73 0.3530124633514973 1104 74-75 0.22603915410840258 1104 76-77 0.2557254912208329 1104 78-79 0.4288838083842812 1104 80-81 0.30717214311658125 1104 82-83 0.24594639758169734 1104 84-85 0.2898710302879053 1104 86-87 0.16692179965145826 1104 88-89 0.42200233693047196 1104 90-91 0.3410363167325272 1104 92-93 0.3108659546038304 1104 94-95 0.22071306758619613 1104 96-97 0.29296246526297054 1104 98-99 0.37603183585068223 1104 100-101 0.3951758293155123 1104 102-103 0.3267097772731269 1104 104-105 0.3870863864945804 1104 106-107 0.31853848992816225 1104 108-109 0.21029531633573129 1104 110-111 0.4195919872367675 1104 112-113 0.3802392329279449 1104 114-115 0.23891289623724532 1104 116-117 0.31792707115937446 1104 118-119 0.38166972525273124 1104 120-121 0.059734907596912024 1104 122-123 -0.12471172296980271 1104 124-125 0.0036176501772260394 1105 1 0.2460882048333488 1105 2 0.6592554876504728 1105 3 0.5067991236120548 1105 4 0.8949165946010176 1105 5 0.7780683329220821 1105 6 0.5817096041988812 1105 7 0.5391035194249802 1105 8 0.48100347764032847 1105 9 0.6612276541384539 1105 10-11 0.6625219938458535 1105 12-13 0.7280157829373088 1105 14-15 0.7574131241315385 1105 16-17 0.9009985334383828 1105 18-19 0.5400851616362843 1105 20-21 0.39610186572684114 1105 22-23 0.2448347908180608 1105 24-25 0.4695752094411887 1105 26-27 0.05719658019377505 1105 28-29 0.21847739291290935 1105 30-31 0.27952233998859555 1105 32-33 0.42338945944420203 1105 34-35 0.3613449580635617 1105 36-37 0.3987528872408035 1105 38-39 0.5291442571904241 1105 40-41 0.5136979549745959 1105 42-43 0.38932106986738546 1105 44-45 0.2841531611876178 1105 46-47 0.36009185702352653 1105 48-49 0.48061703985555226 1105 50-51 0.3487997981528963 1105 52-53 0.2454333482279445 1105 54-55 0.2527477860587197 1105 56-57 0.32920896243679465 1105 58-59 0.19816119460308101 1105 60-61 0.2766454157616671 1105 62-63 0.2307203938006488 1105 64-65 0.3678152072014633 1105 66-67 0.33240190247557777 1105 68-69 0.4009831753451749 1105 70-71 0.11046922704210971 1105 72-73 0.41040370224958167 1105 74-75 0.5067205731459872 1105 76-77 0.3838980036399242 1105 78-79 0.27363266683176946 1105 80-81 0.20618363390889982 1105 82-83 0.25873429461616837 1105 84-85 0.3642927273123391 1105 86-87 0.46188642406813685 1105 88-89 0.3349884375878034 1105 90-91 0.3750445459462348 1105 92-93 0.5312031516533615 1105 94-95 0.3902825392104674 1105 96-97 0.6277283842754677 1105 98-99 0.5351642559420071 1105 100-101 0.6876139847673635 1105 102-103 0.533380959971705 1105 104-105 0.5987201864845453 1105 106-107 0.3843170036518835 1105 108-109 0.4420254583398453 1105 110-111 0.7578427775423506 1105 112-113 0.7329764500487208 1105 114-115 0.5115081092434153 1105 116-117 0.6972210648369277 1105 118-119 0.9935218001185078 1105 120-121 1.1420646782828463 1105 122-123 1.192559590191923 1105 124-125 0.8276579933956505 1106 1 -0.22874357132001322 1106 2 -0.7039869967373846 1106 3 0.1040107225857696 1106 4 -0.353807808748936 1106 5 -0.20183678398743865 1106 6 -0.9465616839842994 1106 7 -0.9119832792389104 1106 8 -0.8940703472132334 1106 9 -0.8239153966433648 1106 10-11 -0.2856432770066455 1106 12-13 -0.33466998944009774 1106 14-15 -0.6323094880580342 1106 16-17 -0.6631734179582409 1106 18-19 -0.49132791867547354 1106 20-21 -0.7197353807271512 1106 22-23 -0.6541195587970918 1106 24-25 -3.920687171349826 1106 26-27 -1.370914955847276 1106 28-29 -0.9275094396377952 1106 30-31 -1.057118992247453 1106 32-33 -5.621430698371547 1106 34-35 -1.8259741105330392 1106 36-37 -1.1380627200593914 1106 38-39 -0.5168667802303091 1106 40-41 -0.7888508178384868 1106 42-43 -0.3875560178026518 1106 44-45 -0.4381492060486707 1106 46-47 -0.7057599123524767 1106 48-49 -0.24386860665652677 1106 50-51 -0.4082268756138667 1106 52-53 -0.3888165428501438 1106 54-55 -0.46132734585782487 1106 56-57 -0.5845394037344818 1106 58-59 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0.3695028733214478 2216 80-81 0.20445262155841704 2216 82-83 0.4647506970927502 2216 84-85 0.16895738418938677 2216 86-87 0.20879556212771533 2216 88-89 0.27087290710059264 2216 90-91 0.3256102044764937 2216 92-93 0.09455690704251296 2216 94-95 0.3208767237869523 2216 96-97 0.15925939014275414 2216 98-99 0.13516425594200854 2216 100-101 0.15122706784947582 2216 102-103 0.17583290330860635 2216 104-105 0.2295218332153972 2216 106-107 0.09964327030558096 2216 108-109 0.23176256489111324 2216 110-111 0.2697556434375201 2216 112-113 0.08359721571373413 2216 114-115 0.09375388706441257 2216 116-117 0.33470688285371963 2216 118-119 0.32651152425867025 2216 120-121 0.3829963130197882 2216 122-123 0.4374978377850418 2216 124-125 0.47830145023380055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15466.0 3 2019.0 4 1488.0 5 1412.0 6 2287.0 7 2671.0 8 2425.0 9 2391.0 10 2936.0 11 3445.0 12 3194.0 13 2783.0 14 2664.0 15 2515.0 16 2242.0 17 1979.0 18 1746.0 19 1464.0 20 1307.0 21 1231.0 22 1381.0 23 2158.0 24 3514.0 25 5060.0 26 5618.0 27 3962.0 28 4140.0 29 5056.0 30 5840.0 31 7112.0 32 9210.0 33 12135.0 34 16741.0 35 24107.0 36 36681.0 37 50170.0 38 18802.0 39 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.986814557441306 18.37015754228102 9.242430991729401 18.400596908548263 2 18.069104785662315 24.644379111170505 37.61637489420464 19.670141208962537 3 17.28429891346714 37.91951415982093 27.102442935680372 17.693743991031557 4 9.508218628892156 18.6274073084176 34.158908340386354 37.705465722303884 5 9.709044203899209 51.30443820808648 28.29376215718592 10.692755430828397 6 24.792584645881206 38.30325888034686 21.796894430688933 15.107262043083 7 27.06160979104566 41.8112840539146 18.03377272271696 13.093333432322776 8 27.660577533437024 38.01259897470925 18.428134987954135 15.898688503899594 9 30.06043417574912 20.10884091111573 16.016170225403144 33.81455468773201 10-11 22.516885715399365 35.12154559746385 24.613888326908192 17.747680360228596 12-13 23.875070299405866 32.99812349308699 24.73573919709262 18.39106701041452 14-15 22.526644418128967 32.7430293896006 23.420731231972557 21.30959496029787 16-17 21.531028543215744 33.9411465545083 24.38182351390781 20.14600138836815 18-19 21.08897200077956 31.463068313643237 27.134836153053744 20.313123532523456 20-21 24.269298374238765 29.3373895386283 26.447436071148168 19.945876015984766 22-23 24.357157055992033 29.947257246787412 25.253913906140564 20.44167179107999 24-25 22.60096627682736 30.259721698909924 26.45178174690823 20.687530277354487 26-27 22.1322231523226 30.25719647122804 26.430338678213932 21.180241698235427 28-29 22.56066551095636 29.106824011552153 27.265792335933064 21.066718141558425 30-31 22.672586279219114 29.51645773552547 27.207511869540845 20.603444115714566 32-33 21.368739710416072 30.043450581228086 26.848041754133927 21.739767954221907 34-35 21.491120614132775 29.93655990615766 27.950762469003816 20.62155701070575 36-37 21.95662019095134 30.18026044218748 26.380165411970836 21.482953954890345 38-39 22.24045287921674 30.469119762424018 26.72711243097768 20.56331492738156 40-41 22.07990348475709 30.562850911790633 26.97081341933089 20.386432184121386 42-43 21.295756662348218 30.834635672842413 26.61876779503094 21.250839869778424 44-45 21.469524270889593 30.79051845582462 26.698999393065353 21.040957880220425 46-47 21.55193374575192 30.82726862704701 26.49074842280515 21.130049204395913 48-49 20.87598394140804 31.02489527206989 26.448604895903127 21.650515890618937 50-51 21.300768040031627 31.015787695768545 26.65700275068953 21.026441513510303 52-53 21.17024732030996 31.55306018282214 26.283884738527213 20.992807758340682 54-55 20.810171221753052 31.464340401837504 26.746415479560113 20.979072896849335 56-57 21.41877407080878 31.693192891281146 25.891141942369263 20.99689109554081 58-59 21.077590687038768 30.80835078028717 26.446612321261526 21.667446211412535 60-61 21.022280131260487 31.8343801505635 26.07465811394717 21.068681604228846 62-63 21.075363416336288 31.4438653540618 26.242631446092624 21.23813978350929 64-65 20.444117778074748 31.872800570181298 26.307964720032075 21.37511693171188 66-67 20.38579969078116 31.954593458889295 25.76316360943396 21.896443240895582 68-69 21.342556674548703 31.29489325844782 25.879675859073682 21.482874207929797 70-71 20.88244137659667 31.047852733407822 26.259980919785143 21.809724970210365 72-73 20.85237333372929 31.8040083299615 25.689801736522277 21.653816599786925 74-75 20.70630203905876 31.92372368582236 25.862043364799604 21.507930910319278 76-77 20.35439457438871 32.09243881524487 26.50980552286842 21.043361087498006 78-79 21.183493015676216 31.28487057749203 25.921437029722817 21.610199377108938 80-81 20.397864051360767 32.06502875957957 25.97382590570867 21.563281283350996 82-83 20.44459870299084 31.415907850728868 25.599226397514375 22.540267048765912 84-85 20.528306649492 30.91013367286712 26.42758241799034 22.133977259650543 86-87 20.3802315243598 31.72685545225576 26.215669933942987 21.677243089441458 88-89 21.09072955972508 30.377688728360713 26.605070372343288 21.92651133957092 90-91 20.705598048914485 30.982391601720195 26.11934065485349 22.192669694511828 92-93 20.143175853505525 31.371919417645117 26.149545824068543 22.33535890478082 94-95 20.03129309528803 30.9736681409934 26.621403660512605 22.373635103205963 96-97 20.39715948743077 31.442194901034938 26.334877574353722 21.825768037180573 98-99 20.066966605787933 31.593463702911784 25.593567642211234 22.746002049089046 100-101 19.663645002746968 32.51258407946902 25.82316213045867 22.000608787325344 102-103 19.949700709943855 32.81759547120783 25.563918147649762 21.668785671198552 104-105 19.14027191313628 33.373486149134614 26.035172381792027 21.45106955593708 106-107 18.812085170831665 34.10322657282433 25.852673467266545 21.232014789077464 108-109 19.103786717349262 34.37761007316557 24.68604879931994 21.832554410165226 110-111 18.947983852257437 34.91049139251079 24.60915966776484 21.53236508746694 112-113 18.987668344543927 34.86272588237041 25.00816665924243 21.14143911384323 114-115 18.535683594511273 35.23702756427984 24.79374583547553 21.433543005733355 116-117 18.912788873978467 34.77884006808062 24.921627475764378 21.386743582176535 118-119 18.902394886157992 34.870344282285856 24.759963843770937 21.467296987785208 120-121 18.489639402196108 34.710229930285905 24.69950183008768 22.100628837430307 122-123 19.298851278738912 34.32725841866612 24.615932869687235 21.757957432907737 124-125 19.383900946031474 34.41386696165575 24.115171954259367 22.087060138053406 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 151.0 1 226.5 2 345.0 3 368.0 4 325.5 5 289.0 6 265.5 7 238.5 8 238.0 9 256.5 10 257.5 11 243.5 12 236.0 13 252.0 14 276.0 15 296.0 16 321.5 17 381.0 18 473.5 19 547.0 20 655.5 21 844.5 22 1058.5 23 1369.0 24 1697.0 25 2003.5 26 2406.0 27 2816.0 28 3201.0 29 3651.0 30 4364.5 31 5064.5 32 5948.0 33 7024.5 34 7858.0 35 8472.0 36 10009.5 37 11572.5 38 11045.5 39 10056.0 40 9508.0 41 8866.0 42 8228.0 43 8188.5 44 8692.5 45 8813.0 46 8282.0 47 7704.5 48 7457.5 49 7111.0 50 7017.0 51 6507.5 52 5539.0 53 5237.5 54 5151.5 55 5204.0 56 5305.0 57 5118.0 58 5038.5 59 5191.0 60 5105.5 61 4968.0 62 4244.5 63 2596.5 64 1493.5 65 1188.5 66 948.5 67 695.0 68 494.0 69 355.5 70 346.0 71 345.0 72 314.0 73 354.0 74 290.5 75 152.0 76 92.0 77 76.5 78 54.0 79 23.0 80 12.0 81 11.5 82 10.0 83 6.0 84 4.5 85 2.5 86 3.5 87 3.5 88 2.0 89 1.5 90 1.5 91 1.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.712104057700945E-4 2 3.712104057700945E-4 3 0.0 4 3.712104057700945E-4 5 3.712104057700945E-4 6 7.42420811540189E-4 7 0.0 8 7.42420811540189E-4 9 0.0018560520288504728 10-11 0.0016704468259654255 12-13 0.0020416572317355198 14-15 0.002969683246160756 16-17 0.002969683246160756 18-19 0.0024128676375056147 20-21 0.002784078043275709 22-23 0.09373062745694888 24-25 0.0016704468259654255 26-27 0.001299236420195331 28-29 7.42420811540189E-4 30-31 7.42420811540189E-4 32-33 9.280260144252364E-4 34-35 3.712104057700945E-4 36-37 3.712104057700945E-4 38-39 5.568156086551418E-4 40-41 5.568156086551418E-4 42-43 7.42420811540189E-4 44-45 9.280260144252364E-4 46-47 9.280260144252364E-4 48-49 1.8560520288504726E-4 50-51 7.42420811540189E-4 52-53 5.568156086551418E-4 54-55 5.568156086551418E-4 56-57 5.568156086551418E-4 58-59 3.712104057700945E-4 60-61 3.712104057700945E-4 62-63 3.712104057700945E-4 64-65 3.712104057700945E-4 66-67 1.8560520288504726E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8560520288504726E-4 82-83 0.0 84-85 0.0 86-87 1.8560520288504726E-4 88-89 1.8560520288504726E-4 90-91 1.8560520288504726E-4 92-93 0.0 94-95 1.8560520288504726E-4 96-97 3.712104057700945E-4 98-99 3.712104057700945E-4 100-101 3.712104057700945E-4 102-103 5.568156086551418E-4 104-105 5.568156086551418E-4 106-107 3.712104057700945E-4 108-109 0.0 110-111 5.568156086551418E-4 112-113 3.712104057700945E-4 114-115 1.8560520288504726E-4 116-117 9.280260144252364E-4 118-119 9.280260144252364E-4 120-121 0.0011136312173102836 122-123 9.280260144252364E-4 124-125 1.8560520288504726E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 269389.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.36633899587323 #Duplication Level Percentage of deduplicated Percentage of total 1 64.13946093036404 25.249357619972773 2 12.540769104752677 9.87368335693335 3 7.388246021472266 8.725445923985669 4 4.84706531765145 7.6324486571922785 5 3.2030853046862013 6.304687096848845 6 1.963172525821882 4.636974909533334 7 1.338942835481974 3.6896494290375377 8 0.8919605479331717 2.809057704070566 9 0.6331321577557962 2.243168563626303 >10 2.820224957345556 19.06026713189882 >50 0.16923340614871776 4.5897947609051855 >100 0.061720418713061775 4.200649764527211 >500 0.0019909812488084445 0.4016854192219788 >1k 9.954906244042223E-4 0.5831296622461507 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1488 0.5523610837859007 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 521 0.19340062140621925 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 504 0.18709004450812766 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 465 0.17261283868309396 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 433 0.16073410569845092 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 410 0.15219626636573877 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 365 0.13549179810608453 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 358 0.13289332526569386 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 313 0.11618885700603958 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 299 0.11099191132525826 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 278 0.1031964928040863 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8560520288504729E-4 0.0 22-23 0.0 0.0 0.0 9.280260144252364E-4 0.0 24-25 0.0 0.0 0.0 0.0018560520288504728 0.0 26-27 0.0 0.0 0.0 0.0018560520288504728 0.0 28-29 0.0 0.0 0.0 0.002784078043275709 0.0 30-31 0.0 0.0 0.0 0.007052997709631796 0.0 32-33 0.0 0.0 0.0 0.022087019143320626 0.0 34-35 0.0 0.0 0.0 0.04621569551837677 0.0 36-37 0.0 0.0 0.0 0.08463597251558155 0.0 38-39 0.0 0.0 0.0 0.1457000842647621 0.0 40-41 0.0 0.0 0.0 0.20713540641971273 0.0 42-43 0.0 0.0 0.0 0.2774797783131457 0.0 44-45 0.0 0.0 0.0 0.37510811503068053 0.0 46-47 0.0 0.0 0.0 0.4831303431097781 0.0 48-49 0.0 0.0 0.0 0.5974631480869672 0.0 50-51 0.0 0.0 0.0 0.7038149293400993 0.0 52-53 0.0 0.0 0.0 0.8272423892586558 0.0 54-55 0.0 0.0 0.0 0.9692303694657168 0.0 56-57 0.0 0.0 0.0 1.1617029648575108 0.0 58-59 0.0 0.0 0.0 1.3809027094647517 0.0 60-61 0.0 0.0 0.0 1.602886532115268 0.0 62-63 0.0 0.0 0.0 1.8146620686071073 0.0 64-65 0.0 0.0 0.0 2.038316338083589 0.0 66-67 0.0 0.0 0.0 2.3386255563515954 0.0 68-69 0.0 0.0 0.0 2.695915571905312 0.0 70-71 0.0 0.0 0.0 3.045966984546511 0.0 72-73 0.0 0.0 0.0 3.372260931218424 0.0 74-75 0.0 0.0 0.0 3.745327389017369 0.0 76-77 0.0 0.0 0.0 4.174632223290484 0.0 78-79 0.0 0.0 0.0 4.720497124975408 0.0 80-81 0.0 0.0 0.0 5.2452030335314355 0.0 82-83 0.0 0.0 0.0 5.747079502132603 0.0 84-85 0.0 0.0 0.0 6.281065670832884 0.0 86-87 0.0 0.0 0.0 6.89059315710738 0.0 88-89 0.0 0.0 0.0 7.566752911217607 0.0 90-91 0.0 0.0 0.0 8.279848100701958 0.0 92-93 0.0 0.0 0.0 8.977166847941081 0.0 94-95 0.0 0.0 0.0 9.720515685495695 0.0 96-97 0.0 0.0 0.0 10.514534743437927 0.0 98-99 0.0 0.0 0.0 11.34753089398602 0.0 100-101 0.0 0.0 0.0 12.364647405796081 0.0 102-103 0.0 0.0 0.0 13.263162192962593 0.0 104-105 0.0 0.0 0.0 14.198241205097462 0.0 106-107 0.0 0.0 0.0 15.199024459053636 0.0 108-109 0.0 0.0 0.0 16.24286812007914 0.0 110-111 0.0 0.0 0.0 17.395105219589517 0.0 112-113 0.0 0.0 0.0 18.457509400903525 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 45 4.0563464E-10 92.54545 1 GTACATA 305 0.0 91.67851 1 GTTCGAC 15 0.0042216093 59.548775 16-17 CATGGGT 235 0.0 55.69605 4 TACATAA 335 0.0 51.50184 2 GTCGGGA 400 0.0 50.56948 2 GTACATG 4770 0.0 50.13918 1 CATTCCG 50 5.328157E-4 47.594803 9 GGGATAG 100 4.130925E-9 47.594803 7 TACATGG 4795 0.0 46.899986 2 CGGGAGT 420 0.0 46.744896 4 GTCACTA 130 1.8189894E-11 45.76423 1 ATGGGAG 1080 0.0 44.06926 5 CATATAG 95 1.483113E-7 43.83732 3 ACGCAAA 150 1.8189894E-12 43.62857 1 ACATGGG 4875 0.0 43.56755 3 TCGGGAG 455 0.0 43.14913 3 CATGGGG 815 0.0 43.06892 4 ACTACCT 140 4.0017767E-11 42.495358 4 TGGGAGT 240 0.0 42.141235 6 >>END_MODULE