##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936195_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 269389 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.354465104365804 31.0 31.0 34.0 30.0 34.0 2 31.24146494474533 31.0 31.0 34.0 28.0 34.0 3 31.524408940231414 31.0 31.0 34.0 28.0 34.0 4 35.42080782808504 37.0 35.0 37.0 33.0 37.0 5 35.134701119941795 37.0 35.0 37.0 33.0 37.0 6 35.40311594014604 37.0 35.0 37.0 33.0 37.0 7 35.205984654161824 37.0 35.0 37.0 32.0 37.0 8 35.31877322385101 37.0 35.0 37.0 32.0 37.0 9 36.89146178945688 39.0 37.0 39.0 33.0 39.0 10-11 36.781436881238655 39.0 37.0 39.0 32.0 39.0 12-13 36.74864415399293 39.0 37.0 39.0 32.0 39.0 14-15 37.61027176313806 39.0 36.0 41.0 32.0 41.0 16-17 37.593277750761914 39.0 36.5 41.0 32.0 41.0 18-19 37.923140885485296 40.0 37.0 41.0 32.5 41.0 20-21 38.021017933174704 40.0 37.0 41.0 33.0 41.0 22-23 37.85182951048484 40.0 37.0 41.0 32.0 41.0 24-25 37.73734636529331 40.0 36.0 41.0 32.0 41.0 26-27 37.48163065307047 40.0 36.0 41.0 32.0 41.0 28-29 37.26232325744555 39.5 36.0 41.0 32.0 41.0 30-31 36.76161795767459 39.0 35.0 40.5 31.0 41.0 32-33 36.129016032577425 38.0 35.0 40.0 30.0 41.0 34-35 35.61597912312678 38.0 35.0 40.0 30.0 41.0 36-37 35.116717831834265 38.0 35.0 40.0 27.0 41.0 38-39 34.8717319563902 38.0 35.0 40.0 25.5 41.0 40-41 34.573622159776384 38.0 35.0 40.5 23.5 41.0 42-43 34.14633671011066 38.0 34.0 41.0 19.5 41.0 44-45 33.463723463096116 38.0 33.5 41.0 7.5 41.0 46-47 32.415237815946455 38.0 33.0 40.5 2.0 41.0 48-49 31.548652691832256 38.0 31.0 40.0 2.0 41.0 50-51 31.184192747291092 38.0 30.5 40.0 2.0 41.0 52-53 30.993535370783512 38.0 30.0 40.0 2.0 41.0 54-55 30.78937521576605 37.0 30.0 40.0 2.0 41.0 56-57 30.614555531220653 37.0 30.0 40.0 2.0 41.0 58-59 30.389421988277174 37.0 29.0 40.0 2.0 41.0 60-61 30.18787515451633 36.0 29.0 40.0 2.0 41.0 62-63 29.972623232574456 36.0 28.0 40.0 2.0 41.0 64-65 29.72237916173266 35.5 28.0 39.5 2.0 41.0 66-67 29.463400510043098 35.0 28.0 39.0 2.0 41.0 68-69 29.195518376771137 35.0 28.0 39.0 2.0 41.0 70-71 28.891764325937586 35.0 27.0 38.5 2.0 40.5 72-73 28.627321457075084 35.0 26.5 38.0 2.0 40.0 74-75 28.443414541796436 35.0 27.0 37.0 2.0 40.0 76-77 28.167430741418542 35.0 26.5 37.0 2.0 39.0 78-79 27.864376422199868 35.0 26.0 36.0 2.0 39.0 80-81 27.598350340956756 34.5 26.0 36.0 2.0 38.5 82-83 27.341597095649785 34.0 26.0 35.5 2.0 37.0 84-85 27.063673720901747 34.0 25.5 35.0 2.0 37.0 86-87 26.87530485654574 34.0 25.0 35.0 2.0 36.5 88-89 26.71745691175215 34.0 25.0 35.0 2.0 36.0 90-91 26.574923252248606 34.0 25.0 35.0 2.0 36.0 92-93 26.417917955076113 34.0 24.0 35.0 2.0 36.0 94-95 26.296452342152058 34.0 24.0 35.0 2.0 35.0 96-97 26.228871631729582 34.0 23.5 35.0 2.0 35.0 98-99 26.15223153135429 34.0 23.0 35.0 2.0 35.0 100-101 26.05351369209582 34.0 22.5 35.0 2.0 35.0 102-103 25.14364914677288 32.5 19.5 34.5 2.0 35.0 104-105 25.791079071528532 34.0 20.0 35.0 2.0 35.0 106-107 25.923925995493505 34.0 20.0 35.0 2.0 35.0 108-109 25.88177319786628 34.0 20.0 35.0 2.0 35.0 110-111 25.828836737951438 34.0 19.0 35.0 2.0 35.0 112-113 25.747725408238644 34.0 19.0 35.0 2.0 35.0 114-115 25.69461076732903 34.0 18.5 35.0 2.0 35.0 116-117 25.5987326876747 34.0 17.5 35.0 2.0 35.0 118-119 25.46654280612794 34.0 10.0 35.0 2.0 35.0 120-121 25.25557650832068 34.0 2.0 35.0 2.0 35.0 122-123 25.020188277917804 34.0 2.0 35.0 2.0 35.0 124-125 23.153191110253204 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3842796058978202 1101 2 0.7131527445677897 1101 3 0.6434511062908754 1101 4 0.3769719462272647 1101 5 0.7411812778696003 1101 6 0.5431448799818597 1101 7 0.5378309212664547 1101 8 0.6069788719523928 1101 9 0.5928437703097345 1101 10-11 0.5777632732037361 1101 12-13 0.6266326012768815 1101 14-15 0.7092896333025536 1101 16-17 0.9221349368594645 1101 18-19 0.908930131698682 1101 20-21 0.7130266651311103 1101 22-23 0.7145093198761572 1101 24-25 0.6640003267253576 1101 26-27 0.6950357968970735 1101 28-29 0.7471747546115424 1101 30-31 0.7222843754556152 1101 32-33 0.5154274563373704 1101 34-35 0.3733352059253505 1101 36-37 0.6285930405449278 1101 38-39 0.567397460009559 1101 40-41 0.4002244174217253 1101 42-43 0.0012443267367885369 1101 44-45 -0.10590427951842685 1101 46-47 0.1778588936140153 1101 48-49 0.11829902269922599 1101 50-51 -0.03283707889086429 1101 52-53 0.0450646909118646 1101 54-55 0.14116734299728506 1101 56-57 0.16817675446253588 1101 58-59 0.18517006484722032 1101 60-61 0.13102745475915256 1101 62-63 -0.04586498591759636 1101 64-65 -0.07780955919432841 1101 66-67 0.1178123857936555 1101 68-69 0.19646487472772378 1101 70-71 0.11678009600574768 1101 72-73 -0.08331202135071436 1101 74-75 -0.07319255525793622 1101 76-77 0.15593387040750173 1101 78-79 0.08694592445952054 1101 80-81 0.07353127790607061 1101 82-83 -0.11479109679051547 1101 84-85 -0.20390229062498832 1101 86-87 -0.17852496886455071 1101 88-89 -0.2538971819361002 1101 90-91 -0.06314091242822073 1101 92-93 -0.09029597675376522 1101 94-95 0.002789176156106521 1101 96-97 -0.14894534348997723 1101 98-99 -0.29738389664066034 1101 100-101 -0.3001493204246586 1101 102-103 0.11028770837535617 1101 104-105 0.03505805752674007 1101 106-107 -0.07422339135234068 1101 108-109 -0.3580834963156079 1101 110-111 -0.2148493394277331 1101 112-113 -0.12457461029621797 1101 114-115 -0.10164052296363124 1101 116-117 -0.13431986358060755 1101 118-119 0.14330945213167823 1101 120-121 -0.004022754490055291 1101 122-123 -0.06542188764274215 1101 124-125 0.2574793551125403 1102 1 0.31946479108300707 1102 2 0.8002912529391786 1102 3 0.5246028059357286 1102 4 0.28843820700858913 1102 5 0.7115643321567688 1102 6 0.3135976958113815 1102 7 0.696728688897096 1102 8 0.6585389937179897 1102 9 0.8195053634096823 1102 10-11 0.7042536334269727 1102 12-13 0.7084597194909747 1102 14-15 0.8456100290407491 1102 16-17 1.0380025167681453 1102 18-19 1.04298327731005 1102 20-21 0.6676023881143749 1102 22-23 0.8406350174915787 1102 24-25 0.8686077595005983 1102 26-27 0.751954873507934 1102 28-29 0.9857116648094149 1102 30-31 1.072106800620503 1102 32-33 0.8277054877934873 1102 34-35 0.6451356879141912 1102 36-37 0.5507772110167721 1102 38-39 0.3784705193702962 1102 40-41 0.3306055691213672 1102 42-43 0.3170421451031018 1102 44-45 0.1255454921710708 1102 46-47 0.41092586469468273 1102 48-49 0.26395795217664997 1102 50-51 -0.004139717145559274 1102 52-53 0.05816083500116065 1102 54-55 0.10051538967409712 1102 56-57 0.10480473518298439 1102 58-59 0.2907636721531546 1102 60-61 0.19359087434311917 1102 62-63 -0.11256653335544797 1102 64-65 -0.01779877786505324 1102 66-67 -0.024643854693408684 1102 68-69 0.24402956270337484 1102 70-71 0.1604444795673885 1102 72-73 0.01333942461580051 1102 74-75 0.02455287853201682 1102 76-77 -0.07768802203288061 1102 78-79 0.0010123881835184534 1102 80-81 0.13661790905776883 1102 82-83 -0.06901801206194946 1102 84-85 -0.18062730330890275 1102 86-87 0.004202707441891107 1102 88-89 -0.03240428492950187 1102 90-91 0.04820802212276831 1102 92-93 -0.046425479544229376 1102 94-95 -0.16043697808031965 1102 96-97 -0.12844496297247332 1102 98-99 -0.1268486353519762 1102 100-101 -0.1073220753713855 1102 102-103 0.0050270538852501545 1102 104-105 0.0660543791908701 1102 106-107 0.08399261575065253 1102 108-109 0.09925097857023957 1102 110-111 0.18521725113543397 1102 112-113 0.01981592750185257 1102 114-115 0.10962596612819908 1102 116-117 0.1323594870028515 1102 118-119 0.028995652030207708 1102 120-121 0.11174969604266849 1102 122-123 0.03587504284939769 1102 124-125 -0.04620532271597355 1103 1 0.09316509689340435 1103 2 0.6354259351896054 1103 3 0.2221123363282551 1103 4 0.14948298938050897 1103 5 0.32532161086314915 1103 6 0.03237780556799663 1103 7 0.4141263680894198 1103 8 0.26383412169754905 1103 9 0.02446116752346228 1103 10-11 0.20414366735935374 1103 12-13 0.08548623702271385 1103 14-15 0.16526567815474635 1103 16-17 0.3846731631591638 1103 18-19 0.35918285001332606 1103 20-21 0.32327768449910366 1103 22-23 0.3242081815818949 1103 24-25 0.14889031992957058 1103 26-27 0.20830881762422138 1103 28-29 0.05792271587556286 1103 30-31 0.08844985285962537 1103 32-33 0.14964424193031078 1103 34-35 0.08785907612582378 1103 36-37 -0.04584630705954851 1103 38-39 0.10333018172210018 1103 40-41 0.28096898079243715 1103 42-43 0.1347602968353243 1103 44-45 -0.01588134373861294 1103 46-47 -0.05175374654908893 1103 48-49 0.3744895595663671 1103 50-51 0.031107025764246288 1103 52-53 -0.22827458873475592 1103 54-55 -0.08101240555861366 1103 56-57 -0.060427136125298375 1103 58-59 0.06361043181969706 1103 60-61 0.256261059923947 1103 62-63 0.07498321319894785 1103 64-65 0.10037808946350424 1103 66-67 0.20933251831149136 1103 68-69 0.28642410000805185 1103 70-71 0.37882138040941626 1103 72-73 0.62323741799689 1103 74-75 0.39058802578181684 1103 76-77 0.13402807699941377 1103 78-79 0.069531721265502 1103 80-81 0.10698990855506807 1103 82-83 -0.07343236420471655 1103 84-85 -0.15352852236131298 1103 86-87 0.21946390303487107 1103 88-89 0.38373873379614665 1103 90-91 0.23926097340256547 1103 92-93 -0.09264264681832657 1103 94-95 0.05364077316596294 1103 96-97 0.14383625691776913 1103 98-99 0.264457761531272 1103 100-101 0.12065852870887994 1103 102-103 0.17090824864039433 1103 104-105 0.06537363686755171 1103 106-107 0.16782341462795003 1103 108-109 0.07052888900549448 1103 110-111 0.33443073192802686 1103 112-113 0.472891835507383 1103 114-115 0.44581318074007115 1103 116-117 0.2916060623453163 1103 118-119 0.5266130538985827 1103 120-121 0.4802407521358347 1103 122-123 0.13238859485811005 1103 124-125 0.27427769014482095 1104 1 0.04320908332044837 1104 2 0.218099472117256 1104 3 0.1273425319631265 1104 4 -0.11567138203250238 1104 5 0.2709250627503721 1104 6 -0.044849792773092645 1104 7 0.3086008350158167 1104 8 0.2978084001106893 1104 9 -0.0024124448094937634 1104 10-11 0.2636143640205759 1104 12-13 0.28722684277865085 1104 14-15 0.0077104856617538076 1104 16-17 0.20352763092338932 1104 18-19 -0.06660576378584437 1104 20-21 0.061438004552726966 1104 22-23 0.006160131646829825 1104 24-25 0.08892739420379314 1104 26-27 0.0944206269325818 1104 28-29 0.16607696161306507 1104 30-31 0.2125177595246157 1104 32-33 0.2809018348254497 1104 34-35 -0.24299216596708106 1104 36-37 -0.3225104602161011 1104 38-39 -0.3486984304013987 1104 40-41 -0.5894857550795507 1104 42-43 -0.33935054896083017 1104 44-45 -0.21007094618509115 1104 46-47 -0.08907413530531727 1104 48-49 -0.09791419394207423 1104 50-51 -0.15025843860674826 1104 52-53 0.10268138294636486 1104 54-55 0.3379583120485279 1104 56-57 0.09909697262590456 1104 58-59 0.1229554529750736 1104 60-61 0.15249498393216143 1104 62-63 0.09291291869934781 1104 64-65 -0.03948827558195234 1104 66-67 0.07010500375407958 1104 68-69 -0.20688367930576135 1104 70-71 0.1341887718048369 1104 72-73 0.12407001822310448 1104 74-75 0.06222411140873163 1104 76-77 0.06386448938264166 1104 78-79 0.22361512790954308 1104 80-81 0.004197817733569309 1104 82-83 -0.08499974722176518 1104 84-85 0.0979115094764822 1104 86-87 0.12520727365193807 1104 88-89 0.021248683107028654 1104 90-91 0.26396144678030353 1104 92-93 0.10083479442837273 1104 94-95 0.16833014520734935 1104 96-97 0.36013951191337057 1104 98-99 0.1631057025475684 1104 100-101 0.18377381503957224 1104 102-103 0.3041138118761211 1104 104-105 0.11285803215890766 1104 106-107 0.2609332550200598 1104 108-109 0.03760714295379941 1104 110-111 0.042523187208491464 1104 112-113 0.07004423800413662 1104 114-115 0.16213738165331293 1104 116-117 -0.017183891992580413 1104 118-119 -0.061186996371613844 1104 120-121 0.10490038097417553 1104 122-123 0.20939102458455494 1104 124-125 0.5953471886995523 1105 1 0.40005087166908737 1105 2 0.7101612915763376 1105 3 0.34884386168363335 1105 4 0.24660971586503422 1105 5 0.5454446071329286 1105 6 0.5887645381015147 1105 7 0.48755110848664174 1105 8 0.3987065886801062 1105 9 0.21386534133130652 1105 10-11 0.3545998625403257 1105 12-13 0.5644810953753705 1105 14-15 0.5865026980156145 1105 16-17 0.7253400650646 1105 18-19 0.6037841203026701 1105 20-21 0.3737943696488202 1105 22-23 0.5178390919559277 1105 24-25 0.3263502453252798 1105 26-27 0.7343774737387534 1105 28-29 0.5876585763453619 1105 30-31 0.4142664674377059 1105 32-33 0.360991151901068 1105 34-35 0.3111073874475352 1105 36-37 0.5218470087989004 1105 38-39 0.8859872035993064 1105 40-41 0.6805027039500118 1105 42-43 0.6352542982467568 1105 44-45 0.5774748413606972 1105 46-47 0.6393668301219506 1105 48-49 0.5367488598990704 1105 50-51 0.6856967021429128 1105 52-53 0.5922920006391763 1105 54-55 0.7603879372178781 1105 56-57 0.9248032298390143 1105 58-59 0.8304997351768897 1105 60-61 0.5946772309089283 1105 62-63 0.7455447617421527 1105 64-65 0.722940847806079 1105 66-67 0.9091661708974357 1105 68-69 0.7959764742393247 1105 70-71 0.8520822938079462 1105 72-73 0.662719724681029 1105 74-75 0.5997698989545164 1105 76-77 0.5374331581567908 1105 78-79 0.6132483257619228 1105 80-81 0.6620055351303265 1105 82-83 0.5608931726437554 1105 84-85 0.6931571314344325 1105 86-87 0.70033899124941 1105 88-89 0.5594066438677281 1105 90-91 0.706356950819643 1105 92-93 0.7443016015938113 1105 94-95 0.9266572062741361 1105 96-97 0.7840293207346853 1105 98-99 0.7568183825616188 1105 100-101 0.8967523889770526 1105 102-103 1.0077617620994097 1105 104-105 1.1452254364441181 1105 106-107 1.2489883506093982 1105 108-109 1.2018752948931848 1105 110-111 0.989645471317079 1105 112-113 1.0042130372414277 1105 114-115 0.9460879060147995 1105 116-117 0.9594632052024608 1105 118-119 0.9978525941748195 1105 120-121 0.8411390281717281 1105 122-123 0.8343822434113903 1105 124-125 1.0483879835211702 1106 1 0.3906835869714058 1106 2 0.6770538703631885 1106 3 0.4587146076777273 1106 4 0.21944216975475683 1106 5 0.562257309848718 1106 6 0.22526064111744404 1106 7 0.2599817615340356 1106 8 0.22182223954906277 1106 9 0.12592420757519562 1106 10-11 0.11148614806096901 1106 12-13 0.5673396905247188 1106 14-15 0.19261684818263092 1106 16-17 0.2589557885481639 1106 18-19 0.25574955574828095 1106 20-21 0.18853755597790922 1106 22-23 0.15076013566992685 1106 24-25 0.18881575338549794 1106 26-27 0.13794043060567418 1106 28-29 0.15669812710297748 1106 30-31 0.08861656667767193 1106 32-33 0.24307470711237755 1106 34-35 0.3054458683498922 1106 36-37 0.14003454813180838 1106 38-39 0.031971615663010766 1106 40-41 0.17891486447670957 1106 42-43 0.33422075029690745 1106 44-45 0.2682697292921432 1106 46-47 -0.4209541264545109 1106 48-49 0.005071946504088487 1106 50-51 -0.02493407676980297 1106 52-53 -0.09913705488679625 1106 54-55 -0.19206401611513257 1106 56-57 -0.22486758392871664 1106 58-59 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122-123 1.0260132975424483 2202 124-125 1.2088682440014296 2203 1 0.4262921003199551 2203 2 0.7867136548756868 2203 3 0.6361998791601309 2203 4 0.29874793908759045 2203 5 0.5534414295875862 2203 6 0.2918229254972502 2203 7 0.4064442635243708 2203 8 0.30902037128304727 2203 9 0.5704653678108542 2203 10-11 0.19087683857633664 2203 12-13 0.4160764879346175 2203 14-15 0.23959418484204065 2203 16-17 0.6710305191887898 2203 18-19 0.5114764279949853 2203 20-21 0.5056889810614962 2203 22-23 0.553612956645452 2203 24-25 0.5260457305629345 2203 26-27 0.2842649356744076 2203 28-29 0.4244254239555829 2203 30-31 0.5207671956251829 2203 32-33 0.7805992546417002 2203 34-35 0.8549310336808205 2203 36-37 1.1177161998488145 2203 38-39 1.0259315965557505 2203 40-41 1.1278878489255106 2203 42-43 1.422557356634158 2203 44-45 1.2578005397586836 2203 46-47 1.304599188125394 2203 48-49 1.2626458544707546 2203 50-51 1.4506245453840165 2203 52-53 1.33602296846653 2203 54-55 1.342249449199869 2203 56-57 1.2355936442438846 2203 58-59 1.2541459684616783 2203 60-61 1.2935386104931972 2203 62-63 1.3570104045330922 2203 64-65 1.4100055902925064 2203 66-67 1.396217673611595 2203 68-69 1.1532252495603892 2203 70-71 1.3639186502226153 2203 72-73 1.3236491566948025 2203 74-75 1.3451558383163587 2203 76-77 1.5356722684440953 2203 78-79 1.6384965714920945 2203 80-81 1.6093774403335601 2203 82-83 1.5637926827721849 2203 84-85 1.4431637513205722 2203 86-87 1.3326837549194757 2203 88-89 1.2749236569751012 2203 90-91 1.3769778961393833 2203 92-93 1.298796504281487 2203 94-95 1.3163963827603027 2203 96-97 1.5714780166171174 2203 98-99 1.6424532296422498 2203 100-101 1.4682074399501772 2203 102-103 1.4694326213606317 2203 104-105 1.3831309044700681 2203 106-107 1.603456773752523 2203 108-109 1.6781363832024638 2203 110-111 1.4193290407596812 2203 112-113 1.4203523196457724 2203 114-115 1.2075601686918809 2203 116-117 1.4467265442730266 2203 118-119 1.4990044543628294 2203 120-121 1.4987717568887895 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56-57 2.0896383946741715 2204 58-59 2.051695488576037 2204 60-61 1.7512240738964486 2204 62-63 1.6589737056795677 2204 64-65 1.800757945962161 2204 66-67 1.616206588107346 2204 68-69 1.5062577184188726 2204 70-71 1.9646917182817774 2204 72-73 1.827368635092654 2204 74-75 1.8251377514410123 2204 76-77 1.6858779337202066 2204 78-79 1.8543546770377652 2204 80-81 1.669977224457952 2204 82-83 1.7207925952550447 2204 84-85 1.8839934137961407 2204 86-87 1.7830732061870158 2204 88-89 1.6237202963169715 2204 90-91 1.7255411564445247 2204 92-93 1.915115533424693 2204 94-95 1.2638335892132346 2204 96-97 1.5644912317050697 2204 98-99 1.6307055123809526 2204 100-101 1.6354001818621313 2204 102-103 1.7133866456903952 2204 104-105 1.8040445833988592 2204 106-107 1.6266750706690019 2204 108-109 1.7377851477521915 2204 110-111 1.9060731114735319 2204 112-113 1.945393890286688 2204 114-115 2.0702078538635575 2204 116-117 1.9565576361951926 2204 118-119 1.7929541028356546 2204 120-121 2.08820552100093 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0.8473806524164367 2205 58-59 0.6312656359427891 2205 60-61 0.8689030051347011 2205 62-63 0.882707598904986 2205 64-65 0.8012292260944562 2205 66-67 0.7342410959723651 2205 68-69 0.883722061984912 2205 70-71 0.7710633127889643 2205 72-73 0.8195628815241882 2205 74-75 0.7889140880987071 2205 76-77 0.883587004310634 2205 78-79 0.8738210863346829 2205 80-81 1.1392283123531044 2205 82-83 1.123497235247818 2205 84-85 1.153097191374492 2205 86-87 0.9945114854219028 2205 88-89 1.1411915256526122 2205 90-91 0.8613686058312986 2205 92-93 0.8424367997290076 2205 94-95 0.985298564915496 2205 96-97 0.9157642524696179 2205 98-99 0.9011074268506647 2205 100-101 0.9778712700959318 2205 102-103 0.9618077161453655 2205 104-105 0.9592114504301357 2205 106-107 0.9598108614319116 2205 108-109 0.9381723311902199 2205 110-111 0.9177506994223066 2205 112-113 1.0289882620166537 2205 114-115 1.2357315956584856 2205 116-117 1.0984242441634997 2205 118-119 0.9271899235121523 2205 120-121 1.1897903768230726 2205 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0.7268266032909558 2206 58-59 0.996678001355157 2206 60-61 1.0467965401710941 2206 62-63 0.8564640012328155 2206 64-65 1.0144287250082407 2206 66-67 0.9208877826190509 2206 68-69 1.1545147613490414 2206 70-71 1.1495181912438426 2206 72-73 1.1604521509848844 2206 74-75 1.0015665150368491 2206 76-77 1.1423520487899665 2206 78-79 1.0195800892365448 2206 80-81 1.0450685039075829 2206 82-83 1.142176968213267 2206 84-85 1.0297522823153002 2206 86-87 1.0636566259956162 2206 88-89 0.8867396283435696 2206 90-91 0.9462836907463839 2206 92-93 0.9812979385901492 2206 94-95 0.9790032729059206 2206 96-97 0.9252468333807684 2206 98-99 0.8092865493155017 2206 100-101 0.9417724482828262 2206 102-103 1.0787542616633417 2206 104-105 0.8346376115705816 2206 106-107 1.0464068051707116 2206 108-109 1.2342492701243017 2206 110-111 1.0625741299600229 2206 112-113 0.862646667103629 2206 114-115 0.845756491397669 2206 116-117 0.8213679671072214 2206 118-119 1.0370641762435469 2206 120-121 0.8884092182990599 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-0.8964880292169859 2216 74-75 -0.7688968325440726 2216 76-77 -0.6890247497170456 2216 78-79 -0.5984441115942332 2216 80-81 -0.5748319636540735 2216 82-83 -0.8419399824017866 2216 84-85 -0.7946512254004716 2216 86-87 -0.4802600557213168 2216 88-89 -0.8179540485372137 2216 90-91 -0.5910108730968524 2216 92-93 -0.7970417158556984 2216 94-95 -0.9060829149615586 2216 96-97 -0.8345709580425833 2216 98-99 -0.7140915334810423 2216 100-101 -0.970073115901549 2216 102-103 -1.0184303348891461 2216 104-105 -0.7044228503723069 2216 106-107 -0.964720359559486 2216 108-109 -1.0795989830412722 2216 110-111 -0.8005487002930245 2216 112-113 -0.725658271390941 2216 114-115 -1.127564400380873 2216 116-117 -1.0966682739378726 2216 118-119 -1.2805065098479993 2216 120-121 -1.3107459599964493 2216 122-123 -1.3742857847256644 2216 124-125 -1.1010301499677624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 29.0 4 67.0 5 144.0 6 353.0 7 750.0 8 1257.0 9 2333.0 10 4296.0 11 5355.0 12 5861.0 13 7510.0 14 10796.0 15 7120.0 16 2476.0 17 1906.0 18 1696.0 19 1465.0 20 1429.0 21 1355.0 22 1450.0 23 1615.0 24 1825.0 25 2126.0 26 2720.0 27 3189.0 28 3826.0 29 4853.0 30 6117.0 31 7505.0 32 9279.0 33 12125.0 34 16255.0 35 23196.0 36 36887.0 37 55167.0 38 24953.0 39 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.610502932752844 17.28113937169624 12.8639938187451 17.244363876805807 2 19.44971360904572 23.86269567573788 36.972459695530965 19.715131019685433 3 18.072749815322823 32.48759229218713 33.934941664284736 15.504716228205309 4 10.222763364502633 17.506282736117658 36.62213379165445 35.64882010772526 5 8.660686094240448 45.083486562285245 33.248639512639066 13.007187830835237 6 25.266436268741487 37.69790154757618 19.23018386051398 17.805478323168355 7 23.3261194777812 37.96405940851334 22.674274005248915 16.035547108456544 8 29.552431613762998 31.286355419115107 21.031296749310478 18.129916217811417 9 29.471136534899344 9.741674678624591 21.145629554287666 39.64155923218839 10-11 24.42898559332415 27.08054152173994 28.442512500510414 20.047960384425494 12-13 27.16016615377762 27.497596412622638 29.98285007925342 15.359387354346318 14-15 23.319252085274456 26.354453968053633 32.40555479251194 17.92073915415997 16-17 17.78565157755435 28.24229690576992 33.304873816068614 20.667177700607116 18-19 17.263139920338247 23.09875310424702 40.96028419868666 18.67782277672808 20-21 19.163180382272476 21.820118861571927 42.5026634346614 16.514037321494197 22-23 18.91187093756612 22.583698666241013 41.537887590064926 16.96654280612794 24-25 17.45839659377332 23.076480479900813 42.27436903511279 17.190753891213078 26-27 17.20077657216887 23.05662072319211 42.254323673201206 17.48827903143781 28-29 17.47361622040989 22.102053164754317 42.75805619383122 17.66627442100457 30-31 18.048992349353536 22.266128164104696 42.39111470772749 17.29376477881428 32-33 17.02575086584827 22.877511702408043 41.909283601037906 18.18745383070578 34-35 17.614741562356155 22.804282299137306 42.076855687706946 17.50412045079959 36-37 18.145321449650876 22.839277030613722 40.049333862926844 18.966067656808555 38-39 18.382524898937966 23.358971598691856 39.69631276703949 18.56219073533069 40-41 18.456766980091984 23.3931229560227 39.40287094127823 18.747239122607084 42-43 18.277272710399025 23.750190250982808 38.09826973691538 19.87426730170279 44-45 18.684996500971746 24.211048019290132 36.642158000152214 20.461797479585908 46-47 19.508011701985474 24.484696832444794 34.612928614918545 21.394362850651184 48-49 19.459799601281542 25.61097849370552 32.46975271288187 22.459469192131067 50-51 20.14649158954141 25.738119368742275 31.718250565214 22.397138476502317 52-53 20.070052604419956 26.4425379312393 30.811637567797334 22.675771896543402 54-55 19.835801447085597 26.644305766439345 31.14408116746916 22.3758116190059 56-57 20.59854253056194 26.160202842919244 30.204996083468526 23.03625854305029 58-59 20.651169179254353 25.291691569807373 31.09935146653203 22.957787784406243 60-61 21.417991617820114 25.904016987092632 30.73286534685074 21.945126048236514 62-63 21.887093630803783 25.72011916141546 31.216764266424136 21.17602294135662 64-65 21.70919896727157 25.627961623484737 31.842521906501325 20.820317502742373 66-67 21.658583305027644 25.893856459543287 31.006513092076077 21.44104714335299 68-69 22.310509249721125 25.896620654709867 30.770658514810506 21.0222115807585 70-71 21.624350595716486 25.43864370790125 31.361656238457353 21.575349457924915 72-73 21.398794277271683 26.08043596082886 30.613775233683022 21.906994528216437 74-75 20.67464029578155 26.61458565340698 30.908392356006946 21.80238169480452 76-77 19.616537515660525 26.903809568001485 31.96733330239896 21.51231961393903 78-79 19.871635441684703 26.277056598450564 31.38528299225284 22.46602496761189 80-81 19.254455485973708 26.870079105524763 31.046412781611586 22.82905262688994 82-83 19.75284811183827 25.93739907717093 30.503658278548862 23.806094532441932 84-85 19.56093233205513 25.854433551481314 30.48509775826036 24.099536358203196 86-87 19.51081892727617 26.438161914554787 30.224508053409753 23.826511104759287 88-89 19.772705759197287 25.649949050898986 30.924712262863952 23.652632927039775 90-91 19.886964753048613 25.942610019117616 30.347458099003283 23.822967128830484 92-93 19.287200455847227 26.272279625893457 30.360984229096637 24.07953568916268 94-95 19.31800464760604 25.851194939603396 30.702226544809307 24.128573867981263 96-97 20.144847644555313 26.068863881449143 30.197504329281532 23.588784144714005 98-99 19.85941974916801 25.712784933004436 29.694469037635933 24.733326280191623 100-101 19.9476960655117 26.03206539291057 29.906045948765158 24.114192592812568 102-103 20.671446628196826 25.62266021006836 29.580141691274868 24.12575147045995 104-105 20.053008944331328 25.849470188965007 30.03728815446836 24.0602327122353 106-107 20.288394434792938 25.90223024039675 29.575556446463835 24.233818878346476 108-109 20.46724687391536 26.105614052671534 28.708565573998694 24.718573499414408 110-111 20.2187922253404 26.20031330274548 28.73903069179028 24.841863780123834 112-113 20.132204956664914 25.809667185809037 28.88869500973624 25.169432847789807 114-115 19.53057413052438 26.38427350935102 28.78542600314114 25.299726356983467 116-117 19.586013570586545 25.859671595518552 29.252875163598898 25.301439670296013 118-119 19.422653543862513 25.996554707020152 28.92746693001377 25.653324819103556 120-121 19.341683712676662 26.213855343413684 28.476179515831447 25.96828142807821 122-123 19.573489449534474 26.340154136287364 28.116322409159082 25.970034005019084 124-125 19.319050836383617 26.293925917126376 27.752864047755168 26.634159198734846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12.0 1 11.5 2 52.0 3 115.0 4 121.0 5 124.0 6 197.5 7 452.5 8 712.0 9 929.5 10 1008.0 11 937.5 12 955.0 13 980.5 14 1015.0 15 1111.0 16 1212.5 17 1296.0 18 1357.5 19 1401.0 20 1493.5 21 1621.0 22 1786.0 23 2009.0 24 2274.5 25 2523.5 26 2770.5 27 3043.0 28 3295.0 29 3743.5 30 4443.0 31 4920.5 32 5571.0 33 6352.5 34 7155.0 35 8162.5 36 9395.0 37 10517.0 38 10128.0 39 9531.0 40 9282.0 41 8488.5 42 7977.0 43 8015.5 44 8507.5 45 8964.5 46 8738.0 47 8272.5 48 8000.0 49 7688.5 50 7809.5 51 6748.0 52 5082.5 53 4591.0 54 4356.5 55 4444.0 56 4565.0 57 4231.0 58 4108.0 59 4228.0 60 4127.5 61 4207.0 62 3691.0 63 2259.0 64 1283.0 65 1035.5 66 820.0 67 586.0 68 459.5 69 327.5 70 286.0 71 298.5 72 243.0 73 283.5 74 255.5 75 160.0 76 105.0 77 57.5 78 38.0 79 19.0 80 6.0 81 4.0 82 4.0 83 2.0 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06978755628477777 2 7.42420811540189E-4 3 0.0 4 0.0 5 0.0683027146616974 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.8560520288504726E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.712104057700945E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002969683246160756 44-45 0.010579496564447694 46-47 0.01224994339041312 48-49 0.009651470550022459 50-51 0.008166628926942079 52-53 0.007424208115401891 54-55 0.007424208115401891 56-57 0.006681787303861702 58-59 0.0044545248692411344 60-61 0.002969683246160756 62-63 0.0024128676375056147 64-65 0.0020416572317355198 66-67 0.003155288449045803 68-69 9.280260144252364E-4 70-71 0.002784078043275709 72-73 0.002598472840390662 74-75 3.712104057700945E-4 76-77 5.568156086551418E-4 78-79 0.0 80-81 7.42420811540189E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.280260144252364E-4 90-91 0.001484841623080378 92-93 1.8560520288504726E-4 94-95 0.0011136312173102836 96-97 9.280260144252364E-4 98-99 0.0020416572317355198 100-101 7.42420811540189E-4 102-103 1.8560520288504726E-4 104-105 1.8560520288504726E-4 106-107 3.712104057700945E-4 108-109 9.280260144252364E-4 110-111 3.712104057700945E-4 112-113 0.012806758999068264 114-115 0.022272624346205673 116-117 0.02097338792601034 118-119 0.013363574607723404 120-121 0.007424208115401891 122-123 0.006310576898091606 124-125 0.005568156086551418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 269389.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.038097323944186 #Duplication Level Percentage of deduplicated Percentage of total 1 58.53529735620948 19.338948509404617 2 13.757148796080942 9.090200416498075 3 8.702149414051528 8.625073778068147 4 5.672969966629588 7.496965354932829 5 3.7628790687745077 6.215918244620234 6 2.369636296221391 4.697296474614776 7 1.5741396164088044 3.640460449387317 8 1.0550443253446589 2.78853256814495 9 0.7752721879529444 2.305216619832287 >10 3.4583881079987866 19.934741210665617 >50 0.22246941045606228 5.047719097661746 >100 0.10561679082257502 6.320599579047399 >500 0.004494331524364895 0.7910493746960715 >1k 0.004494331524364895 3.7072783224259345 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3613 1.3411831960473515 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2485 0.922457858338685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2459 0.9128063877886625 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1430 0.5308308802512351 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 549 0.2037945127677819 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 542 0.2011960399273912 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 531 0.19711272546392022 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.1889460965369781 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 489 0.18152188842157624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 471 0.17484010111771453 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 445 0.1651886305676921 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 420 0.15590837042343972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 401 0.14885537271380792 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 391 0.14514326865610697 No Hit GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT 349 0.12955243161376298 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 330 0.1224994339041312 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 325 0.12064338187528073 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 306 0.11359038416564893 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 303 0.11247675294833864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 296 0.10987828010794798 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 295 0.10950706970217788 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 275 0.10208286158677601 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8560520288504729E-4 0.0 22-23 0.0 0.0 0.0 9.280260144252364E-4 0.0 24-25 0.0 0.0 0.0 0.0018560520288504728 0.0 26-27 0.0 0.0 0.0 0.0018560520288504728 0.0 28-29 0.0 0.0 0.0 0.002784078043275709 0.0 30-31 0.0 0.0 0.0 0.007052997709631796 0.0 32-33 0.0 0.0 0.0 0.021901413940435578 0.0 34-35 0.0 0.0 0.0 0.04695811632991696 0.0 36-37 0.0 0.0 0.0 0.08649202454443203 0.0 38-39 0.0 0.0 0.0 0.14941218832246306 0.0 40-41 0.0 0.0 0.0 0.21158993128895387 0.0 42-43 0.0 0.0 0.0 0.28174869797950175 0.0 44-45 0.0 0.0 0.0 0.37993385030569177 0.0 46-47 0.0 0.0 0.0 0.4872136575732491 0.0 48-49 0.0 0.0 0.0 0.6021032781590934 0.0 50-51 0.0 0.0 0.0 0.7082694542093404 0.0 52-53 0.0 0.0 0.0 0.8328105453452072 0.0 54-55 0.0 0.0 0.0 0.9753553411609235 0.0 56-57 0.0 0.0 0.0 1.169869593784453 0.0 58-59 0.0 0.0 0.0 1.3925958372465097 0.0 60-61 0.0 0.0 0.0 1.6162501067229917 0.0 62-63 0.0 0.0 0.0 1.829510484837911 0.0 64-65 0.0 0.0 0.0 2.055948832357669 0.0 66-67 0.0 0.0 0.0 2.3579284974516406 0.0 68-69 0.0 0.0 0.0 2.7206010638890232 0.0 70-71 0.0 0.0 0.0 3.0764062378196586 0.0 72-73 0.0 0.0 0.0 3.4095675769983185 0.0 74-75 0.0 0.0 0.0 3.7900582429126652 0.0 76-77 0.0 0.0 0.0 4.230684994561768 0.0 78-79 0.0 0.0 0.0 4.784716525173634 0.0 80-81 0.0 0.0 0.0 5.319073904279685 0.0 82-83 0.0 0.0 0.0 5.825776108155864 0.0 84-85 0.0 0.0 0.0 6.365144827739812 0.0 86-87 0.0 0.0 0.0 6.985066205375869 0.0 88-89 0.0 0.0 0.0 7.677188006934211 0.0 90-91 0.0 0.0 0.0 8.397707404533964 0.0 92-93 0.0 0.0 0.0 9.112473040844282 0.0 94-95 0.0 0.0 0.0 9.875310424701826 0.0 96-97 0.0 0.0 0.0 10.694757395439309 0.0 98-99 0.0 0.0 0.0 11.547613302696101 0.0 100-101 0.0 0.0 0.0 12.593313015750457 0.0 102-103 0.0 0.0 0.0 13.51168755962567 0.0 104-105 0.0 0.0 0.0 14.476649009425032 0.0 106-107 0.0 0.0 0.0 15.504530623002424 0.0 108-109 0.0 0.0 0.0 16.570646908374137 0.0 110-111 0.0 0.0 0.0 17.752580840346116 0.0 112-113 0.0 0.0 0.0 18.845053064527505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2410 0.0 74.423706 1 GTATTAC 30 0.0042082113 59.588417 1 CCACGCA 40 1.7663001E-4 59.52201 4 TACCTGG 190 0.0 56.38927 2 GGGATTC 185 0.0 51.449825 7 GGATACG 35 0.007795578 50.990456 8 GTATCAA 3825 0.0 46.99515 2 GCTTACT 130 1.8189894E-11 45.760662 8 GGCCTAA 55 8.4547023E-4 43.33703 1 GATTATC 55 8.524615E-4 43.26463 9 CTTACTA 110 9.600626E-9 43.26463 9 GTCGGGA 430 0.0 42.911213 2 GTACATG 4445 0.0 42.496124 1 CGGGAGT 475 0.0 41.352135 4 TACATGG 4605 0.0 40.71538 2 ATGGGAT 1260 0.0 40.626133 5 CATGGGG 645 0.0 40.604164 4 ACATGGG 4525 0.0 40.514427 3 TCAGCCG 30 0.0013079032 39.666607 74-75 GGGTATG 60 0.0013079332 39.659245 7 >>END_MODULE